Effects of Some Hormone Applications on Germination and Morphological Characters of Endangered Plant Species Lilium artvinense L. Seeds

Lilies are economically important plants because of their large and attractive flowers. Thus, many wild species of lilies have been cultivated to produce Lilium bulbs or flowers. This work was conducted to analyse the effect of hormone applications on Lilium artvinense (Syn: Lilium ponticum K. Koch., Lilium ponticum var. artvinense (Miscz.) P. H. Davis and D. M. Hend., Lilium carniolicum var. artvinense (Miscz.) P. H. Davis and D. M. Hend and Lilium pyrenaicum var. artvinense (Miscz.) V.A. Matthews) seeds on germination percentage and seedlings morphological traits. In the research, 1000, 3000 and 5000 ppm doses of IAA, IBA, NAA and GA3 hormones were applied to L. artvinense seeds and approximately 180 days later, the number of roots, root length, offset stem height and diameter were assessed. As a result, while the control group except 5000 ppm NNA application achieved an increase in the percentage of germination (40%) of all the applications. Germination frequency up to 100% was obtained using 5000 ppm GA3. Effects of hormone applications on other key morphological characters (rooting percentage, root height, number of scions, scion height and width) are described in terms of growth rate between 1.27 and 2.44

Calcific mediopatellar plica: a case report

A 38-year-old male patient was admitted to our clinic with the complaints of pain and swelling in his right knee for two years. He had mechanical symptoms for nearly six months. Merchant and lateral knee X-ray revealed calcification in the medial patellar retinaculum. Magnetic resonance imaging demonstrated increased ossification and cortical irregularity in the patella's medial and inferior pole. Due to long-term complaints of the patient, arthroscopy was planned with the preliminary diagnoses of patellofemoral chondromalacia, cartilage degeneration and mediopatellar plica (MPP) syndrome. Arthroscopy revealed a calcific MPP, an interesting entity. It should be kept in mind that plica may become calcific in patients with suspected MPP and the complaints of the patient may not disappear within if left untreated

Assessment of clinical, biochemical, and radiological outcomes following intra-articular injection of Wharton jelly-derived mesenchymal stromal cells in patients with knee osteoarthritis: A prospective clinical study

The aim of the present study was to perform clinical, biochemical, and radiological evaluation of the efficacy of mesenchymal stem cells derived from Wharton jelly (WJ) present within the human umbilical cord in the treatment of knee osteoarthritis. Between 2018 and 2019, 10 patients with knee osteoarthritis for whom the conservative treatment was not beneficial were included in the study. Patients were clinically, radiologically, and biochemically evaluated before treatment initiation. Thereafter, the patients were intra-articularly injected using a solution containing 1 x 10(8) WJ-derived MSCs. Evaluations were performed on day 21 (V1) and 42 (V2) and month 3 (V3), 6 (V4), and 12 (V5) after the procedure. At 1-year post-injection, visual analogue scale, Western Ontario and McMaster Universities Osteoarthritis Index, and Lequesne scores of patients were lower than those observed during the initial evaluation, whereas the mean 36-Item Short Form Health Survey score was higher. Cartilage thicknesses were found to be increased in all regions except in the medial femur, medial posterior femur, lateral posterior femur, and lateral posterior tibia regions in magnetic resonance imaging. A significant increase was observed in tumor necrosis factor-alpha, interleukin-1 beta, adiponectin, resistin, and interleukin-6 levels compared with pre-injection values. The leptin levels at 6-month and 1-year controls were lower than the pre-injection levels, and the decrease observed at 6 months was significant. In patients with knee osteoarthritis, intra-articular WJ-derived MSC injection causes significant pain reduction, satisfactory functional improvement, and increased patient satisfaction following a 1-year follow-up. These clinical improvements were supported by magnetic resonance images, along with changes in adiponectin and leptin levels in synovial fluid. Level of evidence: IV