4 research outputs found
Blood mRNA expression profiles of autophagy, apoptosis, and hypoxia markers on blood cardioplegia and custodiol cardioplegia groups
Introduction: Blood cardioplegia (BC) and Custodiol cardioplegia
(CC) have been used for a long time in open heart surgery and
are highly effective solutions. The most controversial issue among
these two is whether there is any difference between them
regarding myocardial damage after ischemia surgery. In this study,
autophagy, apoptosis, and hypoxia markers were investigated
and that way we evaluated the differences between BC and CC
patients
Overexpression of the PTEN Gene in Myocardial Tissues of Coronary Bypass Surgery Patients
Effects of hydroxyethyl starch 130/0.4 on the kidney tissue of rats with ureteral obstruction
Objective: This study was conducted since the effects of colloid
solutions on the renal system remain controversial and need to be
adequately studied in animals. We aimed to evaluate the effects of
hydroxyethyl starch (Voluven) on the kidney tissue of rats with late
renal failure due to ureteral obstruction.
Materials and methods: Rats were divided into four groups: Group C,
control; Group HES, hydroxyethyl starch solution (HES) 130/0.4 (Voluven
(R)); Group UUO, unilateral ureteral obstruction (UUO); and Group
UUO-HES, UUO-HES 130/0.4 (Voluven (R)). In the groups with ureteral
obstruction, the distal part of the right ureter was accessed and
sutured through a lower abdominal incision under ketamine anesthesia.
Any signs of late-stage renal failure were evaluated after three weeks.
Rats in the HES group and the renal failure-HES group were administered
with HES 130/0.4 as a single intravenous dose of 20 mL/kg. After a
follow-up of 24 hours, intra-abdominal blood sample was collected, and
the rats were sacrificed. Biochemical and histopathological parameters
were then evaluated.
Results: Ureteral obstruction significantly increased urea and
creatinine levels. In addition, when the UUO-HES and HES groups were
compared, the administration of HES increased urea and creatinine levels
in the UUO-HES group. Nitric oxide enzyme activity and malondialdehyde
levels have significantly increased in the UUO groups. In addition, HES
significantly increased nitric oxide activity and malondialdehyde levels
in the UUO-HES group, in comparison with the LIES group. The activity of
caspases 3 and 8 was significantly increased in the UUO groups. In
addition, HES significantly increased the activity of caspases 3 and 8
in the UUO-HES group, in comparison with the HES group. Light microscopy
revealed significant changes in the UUO groups, especially in the
obstructed kidneys.
Conclusion: If indicated, HES should be used with caution in cases of
UUO, but not in the cases of bilateral ureteral obstruction. Other
aspects of these findings, including the clinical significance and
practical applications, merit further experimental and clinical
investigation