Cloning and characterization of Salmonella typhimurium dehydroquinate synthase

SIGLEAvailable from British Library Document Supply Centre-DSC:DXN024244 / BLDSC - British Library Document Supply CentreGBUnited Kingdo

Hereditary haemochromatosis gene (HFE) H63D mutation shows an association with abnormal sperm motility

The aim of this study was to screen infertile men for HFE H63D mutation in correlation with clinical characteristics of infertile men (sperm concentration, sperm motility, morphology, testicular volume, Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and total Testosterone levels) and find out if the HFE H63D mutation has an effect on male infertility. After excluding hormonal treatment, any scrotal pathology, having any systemic diseases such as diabetes mellitus, sickle cell anemia and microdeletions of the Y chromosome, a total of 148 infertile men with age range between 17 and 52-years-old (average age 29.6 ± 7.2) were enrolled into the study. Our analysis indicates that the mean FSH levels are significantly higher (6.3 ± 4.6 mIU/ml, P = 0.03), whereas sperm motility is significantly lower (36.6 ± 28.1%, P = 0.01) in the infertile men with the HFE H63D mutation compared with subjects lacking this mutation. Comparison of allele frequencies of the infertile men with Ts 50% revealed a significant difference as expected (P = 0.001, OR = 0.14, %95 CI = 0.04-0.44). Comparison of allele frequencies of infertile men with abnormal sperm motility versus infertile men with normal sperm motility revealed a highly significant difference (P = 0.005, OR = 3.11, %95 CI = 1.41-6.86). Thus, the HFE H63D mutation seems to be an important risk factor for impaired sperm motility and is clinically associated with male infertility. © 2008 Springer Science+Business Media B.V.KÜ AF 03/08.01.03Acknowledgments This work was supported by Kirikkale University Research Found (KÜ AF 03/08.01.03). We thank the infertility patients who made this analysis possible

pTARGET vector carrying IL-18 or CD40L genes for attenuated Salmonella typhimurium mediated gene therapy.

45th Annual Meeting of the American-Society-of-Hematology -- DEC 06-09, 2003 -- SAN DIEGO, CALIFORNIAEsendagli, Gunes/0000-0003-4865-2377;WOS: 000186537101986…Amer Soc Hemato

Coexistence of different tissue tumourigenesis in an N-methyl-N-nitrosourea-induced mammary carcinoma model: a histopathological report in Sprague-Dawley rats

Esendagli, Gunes/0000-0003-4865-2377;WOS: 000262991000009PubMed: 18987061N-methyl-N-nitrosourea (MNU), a highly potent carginogen, is widely used to generate mammary tumours in murine species. In a model of MNU-induced mammary carcinogenesis using immature female Sprague-Dawley rats, large mammary tumours (largest dimension >= 0.5 cm) were obtained within a very short period of time. In addition, in the rats bearing MNU-induced mammary carcinomas, there were a number of tumours whose origins were not from mammary tissue but from several different tissues and from mammary non-epithelial tissue. The tumours were of mesenchymal or epithelial origin and they were located in the inguinal region. These tumours were diagnosed as fibroadenoma, combined tubular adenoma and fibroadenoma, hyperkeratotic papilloma, keratinous cyst and malignant peripheral nerve sheath tumour (MPNST) with smooth muscle differentiation. The occurrence of these other tumours in addition to the development of the mammary carcinomas may be attributed to a direct local effect of the intraperitoneal administration of MNU during the sexual development of the immature rats. In the MNU-induced mammary tumour model, coexistence of tumourigenesis in various non-mammary tissues should be considered an important factor that may interfere with experimental procedures and results and also the quality of life of the tumour-bearing animals.Eczacibasi Scientific Research and Award Fund; Hacettepe University Scientific Research UnitHacettepe University [05DO3104001]This study was supported by grants from the Eczacibasi Scientific Research and Award Fund and the Hacettepe University Scientific Research Unit (project no. 05DO3104001). We Wish to thank Dr Sibel Percinel for her helpful supervision in the histopathological evaluations

Salmonella typhimurium aroB-encoding murine IL-18 or CD40L: evaluation for gene therapy

31st Congress of the Federation-of-European-Biochemical-Societies (FEBS) -- JUN 24-29, 2006 -- Istanbul, TURKEYEsendagli, Gunes/0000-0003-4865-2377;WOS: 000238914000163…Federat European Biochem So

Aromatic Compound-Dependent Brucella suis Is Attenuated in Both Cultured Cells and Mouse Models

The aroC gene of the facultative intracellular pathogen Brucella suis was cloned and sequenced. The cloned aroC gene complements Escherichia coli and Salmonella enterica serovar Typhimurium aroC mutants. A B. suis aroC mutant was found to be unable to grow in a defined medium without aromatic compounds. The mutant was highly attenuated in tissue culture (THP1 macrophages and HeLa cells) and murine virulence models