Recuperação e sobrevivência de Bradyrhizobium em sementes de soja tratadas com fungicidas e inseticidas.

O processo de fixação biológica do nitrogênio (FBN) representa um componente essencial para a viabilidade econômica da cultura da soja. No entanto, para um processo eficiente, um número mínimo de células viáveis de Bradyrhizobium deve estar presente para o estabelecimento da simbiose e o uso concomitante de produtos químicos, como inseticidas e fungicidas, pode comprometer a viabilidade das células. Neste estudo, foram avaliadas novas combinações de inoculantes e polímeros na presença de tratamento de sementes com fungicidas e inseticidas, em tratamento manual ou industrial. Foi constatado que novas formulações e aplicações de produtos nas sementes podem permitir a pré-inoculação por até 4 dias, considerando a sobrevivência de pelo menos 10 5 células/semente

Propensity to consent to data linkage: experimental evidence on the role of three survey design features in a UK longitudinal panel

When performing data linkage, survey respondents need to provide their informed consent. Since not all respondents agree to this request, the linked data-set will have fewer observations than the survey data-set alone and bias may be introduced. By focusing on the role that survey design features play in gaining respondents’ consent, this paper provides an innovative contribution to the studies in this field. Analysing experimental data collected in a nationally representative household panel survey of the British population, we find that interview features such as question format (dependent/independent questions) and placement of the consent question within the questionnaire have an impact on consent rates

Desenvolvimento de formulações líquidas de inoculantes contendo Rhizobium spp. para a cultura do feijoeiro.

No Brasil, a cultura do feijoeiro (Phaseolus vulgaris L.) apresenta grande importância social e econômica, além de representar a principal fonte proteica da dieta da população. Contudo, o cultivo do feijoeiro ainda apresenta um baixo rendimento médio nacional, em geral devido a práticas agrícolas inadequadas, principalmente quanto ao suprimento de nitrogênio. Contudo, melhorias consideráveis de produtividade com baixo custo podem ser obtidas com a prática de inoculação com estirpes elite de rizóbios eficientes no processo de fixação biológica do nitrogênio. A simbiose do feijoeiro com bactérias do gênero Rhizobium selecionadas pela pesquisa brasileira, como Rhizobium tropici estirpe SEMIA 4077 (=CIAT 899) e Rhizobium freirei estirpe SEMIA 4080 (=PRF 81) é capaz de suprir, de forma econômica e ecológica, o nitrogênio necessário para rendimentos duas ou mais vezes superiores à média nacional. A principal limitação ao uso de inoculantes na cultura do feijoeiro consiste na preferência dos agricultores por formulações líquidas pela facilidade de aplicação, mas nesse caso a sobrevivência dessas bactérias cai drasticamente, não mantendo as concentrações estipuladas pela legislação brasileira após 1 ou 2 meses de armazenamento. O objetivo deste estudo é desenvolver formulações líquidas de inoculantes para essas duas estirpes de Rhizobium, visando o aumento da viabilidade de células e tempo de prateleira para pelo menos 180 dias. O desenvolvimento dos meios de cultivo baseia-se em um planejamento fatorial 33, com três réplicas no ponto central para o estudo da produção de biomassa com variação nas fontes de carbono, nitrogênio e protetores celulares. O controle de qualidade segue as metodologias estabelecidas na legislação brasileira pelo MAPA (Instrução Normativa nº 30 de 2010) para contagem de Unidades Formadoras de Colônia (UFC). Será acompanhada a viabilidade do inoculante por no mínimo 6 meses, visando a manutenção de pelo menos 109 células de Rhizobium por mililitro e ausência de contaminantes na diluição de 105. Até o presente momento já foram identificadas três formulações, com variações nas fontes de carbono e nitrogênio, sendo constatadas diferenças entre as espécies, com maior sobrevivência de R. freirei SEMIA 4080 na FORM 1, enquanto que R. tropici SEMIA 4077 apresentou maior sobrevivência nas FORM 1 e FORM 2

Bartonella seropositivity in children with Henoch-Schonlein purpura

BACKGROUND: An association between Henoch-Schonlein purpura (HSP) and seropositivity for Bartonella henselae (BH) has been described. The objective of this study was to see if such an association exists in northern Alberta. METHODS: Immunofluorescent antibody testing utilizing an antigen prepared from B. henselae was undertaken on sera from six children with current HSP, 22 children with remote HSP, and 28 controls that were matched for age. Blood from the six children with current HSP was analysed by polymerase chain reaction (PCR) assay with primers derived from the citrate synthase (gltA) gene for the detection of Bartonella DNA. RESULTS: The seropositivity rate for BH was 61% in cases versus 21% in controls (p < 0.03). The PCR assay was negative in all six current cases. CONCLUSION: There is an increased seropositivity rate for BH in children with HSP. However, it is not clear if infection with B. henselae or a related Bartonella species can result in HSP, or if the increased seropositivity is from non-specific or cross-reacting antibodies

Analysis of four studies in a comparative framework reveals: health linkage consent rates on British cohort studies higher than on UK household panel surveys

Background: A number of cohort studies and longitudinal household panel studies in Great Britain have asked for consent to link survey data to administrative health data. We explore commonalities and differences in the process of collecting consent, achieved consent rates and biases in consent with respect to socio-demographic, socio-economic and health characteristics. We hypothesise that British cohort studies which are rooted within the health sciences achieve higher consent rates than the UK household longitudinal studies which are rooted within the social sciences. By contrast, the lack of a specific health focus in household panel studies means there may be less selectivity in consent, in particular, with respect to health characteristics. Methods: Survey designs and protocols for collecting informed consent to health record linkage on two British cohort studies and two UK household panel studies are systematically compared. Multivariate statistical analysis is then performed on information from one cohort and two household panel studies that share a great deal of the data linkage protocol but vary according to study branding, survey design and study population. Results: We find that consent is higher in the British cohort studies than in the UK household panel studies, and is higher the more health-focused the study is. There are no systematic patterns of consent bias across the studies and where effects exist within a study or study type they tend to be small. Minority ethnic groups will be underrepresented in record linkage studies on the basis of all three studies. Conclusions: Systematic analysis of three studies in a comparative framework suggests that the factors associated with consent are idiosyncratic to the study. Analysis of linked health data is needed to establish whether selectivity in consent means the resulting research databases suffer from any biases that ought to be considered

Evidence of Transfer by Conjugation of Type IV Secretion System Genes between Bartonella Species and Rhizobium radiobacter in Amoeba

Background: Bartonella species cospeciate with mammals and live within erythrocytes. Even in these specific niches, it has been recently suggested by bioinformatic analysis of full genome sequences that Lateral Gene Transfer (LGT) may occur but this has never been demonstrated biologically. Here we describe the sequence of the B. rattaustraliani (AUST/NH4 T) circular plasmid (pNH4) that encodes the tra cluster of the Type IV secretion system (T4SS) and we eventually provide evidence that Bartonella species may conjugate and exchange this plasmid inside amoeba. Principal Findings: The T4SS of pNH4 is critical for intracellular viability of bacterial pathogens, exhibits bioinformatic evidence of LGT among bacteria living in phagocytic protists. For instance, 3 out of 4 T4SS encoding genes from pNH4 appear to be closely related to Rhizobiales, suggesting that gene exchange occurs between intracellular bacteria from mammals (bartonellae) and plants (Rhizobiales). We show that B. rattaustraliani and Rhizobium radiobacter both survived within the amoeba Acanthamoeba polyphaga and can conjugate together. Our findings further support the hypothesis that tra genes might also move into and out of bacterial communities by conjugation, which might be the primary means of genomic evolution for intracellular adaptation by cross-talk of interchangeable genes between Bartonella species and plant pathogens. Conclusions: Based on this, we speculate that amoeba favor the transfer of genes as phagocytic protists, which allows fo

Microbial Prevalence, Diversity and Abundance in Amniotic Fluid During Preterm Labor: A Molecular and Culture-Based Investigation

BACKGROUND: Preterm delivery causes substantial neonatal mortality and morbidity. Unrecognized intra-amniotic infections caused by cultivation-resistant microbes may play a role. Molecular methods can detect, characterize and quantify microbes independently of traditional culture techniques. However, molecular studies that define the diversity and abundance of microbes invading the amniotic cavity, and evaluate their clinical significance within a causal framework, are lacking. METHODS AND FINDINGS: In parallel with culture, we used broad-range end-point and real-time PCR assays to amplify, identify and quantify ribosomal DNA (rDNA) of bacteria, fungi and archaea from amniotic fluid of 166 women in preterm labor with intact membranes. We sequenced up to 24 rRNA clones per positive specimen and assigned taxonomic designations to approximately the species level. Microbial prevalence, diversity and abundance were correlated with host inflammation and with gestational and neonatal outcomes. Study subjects who delivered at term served as controls. The combined use of molecular and culture methods revealed a greater prevalence (15% of subjects) and diversity (18 taxa) of microbes in amniotic fluid than did culture alone (9.6% of subjects; 11 taxa). The taxa detected only by PCR included a related group of fastidious bacteria, comprised of Sneathia sanguinegens, Leptotrichia amnionii and an unassigned, uncultivated, and previously-uncharacterized bacterium; one or more members of this group were detected in 25% of positive specimens. A positive PCR was associated with histologic chorioamnionitis (adjusted odds ratio [OR] 20; 95% CI, 2.4 to 172), and funisitis (adjusted OR 18; 95% CI, 3.1 to 99). The positive predictive value of PCR for preterm delivery was 100 percent. A temporal association between a positive PCR and delivery was supported by a shortened amniocentesis-to-delivery interval (adjusted hazard ratio 4.6; 95% CI, 2.2 to 9.5). A dose-response association was demonstrated between bacterial rDNA abundance and gestational age at delivery (r(2) = 0.42; P<0.002). CONCLUSIONS: The amniotic cavity of women in preterm labor harbors DNA from a greater diversity of microbes than previously suspected, including as-yet uncultivated, previously-uncharacterized taxa. The strength, temporality and gradient with which these microbial sequence types are associated with preterm delivery support a causal relationship

The Population Structure of Acinetobacter baumannii: Expanding Multiresistant Clones from an Ancestral Susceptible Genetic Pool

Outbreaks of hospital infections caused by multidrug resistant Acinetobacter baumannii strains are of increasing concern worldwide. Although it has been reported that particular outbreak strains are geographically widespread, little is known about the diversity and phylogenetic relatedness of A. baumannii clonal groups. Sequencing of internal portions of seven housekeeping genes (total 2,976 nt) was performed in 154 A. baumannii strains covering the breadth of known diversity and including representatives of previously recognized international clones, and in 19 representatives of other Acinetobacter species. Restricted amounts of diversity and a star-like phylogeny reveal that A. baumannii is a genetically compact species that suffered a severe bottleneck in the recent past, possibly linked to a restricted ecological niche. A. baumannii is neatly demarcated from its closest relative (genomic species 13TU) and other Acinetobacter species. Multilocus sequence typing analysis demonstrated that the previously recognized international clones I to III correspond to three clonal complexes, each made of a central, predominant genotype and few single locus variants, a hallmark of recent clonal expansion. Whereas antimicrobial resistance was almost universal among isolates of these and a novel international clone (ST15), isolates of the other genotypes were mostly susceptible. This dichotomy indicates that antimicrobial resistance is a major selective advantage that drives the ongoing rapid clonal expansion of these highly problematic agents of nosocomial infections