IDENTIFYING AND MAPPING SALT-AFFECTED LANDS IN HAMBANTOTA DISTRICT, SRI LANKA: AN INTEGRATED GIS AND REMOTE SENSING APPROACH

Since available lands in Sri Lanka is decreasing every year mainly due to degradation oflands, it is very important to restore these lands for useful agricultural production. In thisregard, identification of salt-affected lands and differentiates into salinity levels wouldhelp to take necessary actions to restore these lands.An extensive soil survey was conducted throughout the Hambantota district in order to10 0 to to KA•••••.•.identify the salt-affected lands in the district The sampling was done at different depths(i.e. O-lOcm, IO-20cm, 20-30cm, 30-4Ocm and 40-50cm) by using a manual soil auger.The distance between sampling sites was approximately 3km. The exact position ofsampling points in latitude and longitl!c!~ ~ identified by GP~ (Global PositioningSystea), A total number of 100 points and 400 samples were collected. Topographicmaps of survey of Sri Lanka used in the study were Timbolketiya (82), Kataragama(83),Yala(l4), Hambantota(88) and Tissamaharama(89) of scale 1:50,000. Digital thematicmaps of same map sheets and Scale of I :25000 digital maps were also been used.The samples were taken to the laboratory of the Department of Crop Science, Faculty ofAgriallture, University of Ruhuna, where EC and pH measurements were taken using theEC meter and the pH meter. AIl the locational data taken using GPS system, entered intoa database. EC and pH data also been entered to the database. Using ArcView software, apoint map was generated using all the locational data including EC and pH values asattribstes. By applying interpolating techniques, salinity maps were produced.

Comparative study of thermal efficiency in five different fuel wood cook stoves for conserving biomass energy sources in Sri Lanka

Minimum energy requirements for cooking and heating may be estimated as 6 - 10 GJ, or 0.5 - 1 m³ of fuel wood per person per year. Inefficient use of biomass for anthropogenic activities causes reduction of biomass availability. Therefore, there is a scientific need to identify most efficient cook stoves to improve the efficiency of using bio mass for cooking for the sake of saving forest cover and for the solution of the green energy requirement.The present research study formulated to evaluate the efficiency of existing cook stoves such as “Batapola Lipa” (BS), “Anagi Lipa” (AS), and traditional three stone cook stove (TS) with newly developed cook stoves such as traditional three stone cook stove with grater (TSG) and a prototype metal stove (PMS) developed by the Department of Agricultural Engineering, Faculty of Agriculture, University of Ruhuna. The standard boiling water laboratory test was done for studying the thermal efficiency. Cinnamon sticks were used as the source of biomass. Initial and boiling water temperatures, amount of water boiled and evaporated, amount of biomass utilized and unburned remaining biomass in weight basis after the boiling test, were measured and percent heat utilized and power output of stoves were calculated.The results which were analyzed using ANOVA procedure with Duncan’s Multiple Range Test, revealed that energy output of the BS, AS, TS, TSG and PMS, were observed as 15.1 kW, 5.8 kW, 3.9 kW, 4.7 kW and 14.3 kW respectively. Comparatively higher percent heat utilized value were observed in TSG, AS and BS than TS and PMS. The BS, TS, TSG, PMS consumed 190, 274, 172, 298 g, within 3.95, 22, 11.25, 6.52 minutes respectively. Lowest biomass consumption rate was observed in TSG. It revealed that 102 g of biomass and 10.75 minutes can be saved by TSG than TS for boiling one liter of water. Therefore the significant amount of fuel wood can be saved successfully by introducing a grater to the traditional three stone cook stove which may save natural forests resources in Sri Lanka

Lack of evidence of disease contamination in ovarian tissue harvested for cryopreservation from patients with Hodgkin lymphoma and analysis of factors predictive of oocyte yield

Ovarian cryopreservation is a promising technique to preserve fertility in women with Hodgkin lymphoma (HL) treated with chemotherapy. Thus, the aim of this study was to examine harvested ovarian tissue for subclinical involvement by HL by morphology/immunohistochemistry, and to define patient and treatment factors predictive of oocyte yield. This was a retrospective analysis of 26 ovarian tissue samples harvested for cryopreservation from women with HL. Histology, immunohistochemistry and follicle density (number mm−3) was examined. Disease status and preharvest chemotherapy details were obtained on 24 patients. The median age was 22 years (range 13–29). Seven of 24 patients had infradiaphragmatic disease at time of harvest. Nine of 20 patients had received chemotherapy preharvest (ABVD (Adriamycin®, Bleomycin, Vinblastine and Dacarbazine)=7, other regimens=2). The seven receiving ABVD showed no difference in follicle density compared to patients not receiving treatment (n=14); (median=1555 vs 1620 mm3 P=0.97). Follicle density measurement showed no correlation with patient age (R2=0.0001, P=0.99). There was no evidence of HL involvement in the 26 samples examined (95% CI=0–11%). In conclusion, subclinical involvement of HL has not been identified in ovarian tissue, even when patients have infradiaphragmatic disease. Furthermore, the quality of tissue harvested does not appear to be adversely affected by patient's age or prior ABVD chemotherapy

Increased Terpenoid Accumulation in Cotton (Gossypium hirsutum) Foliage is a General Wound Response

The subepidermal pigment glands of cotton accumulate a variety of terpenoid products, including monoterpenes, sesquiterpenes, and terpenoid aldehydes that can act as feeding deterrents against a number of insect herbivore species. We compared the effect of herbivory by Spodoptera littoralis caterpillars, mechanical damage by a fabric pattern wheel, and the application of jasmonic acid on levels of the major representatives of the three structural classes of terpenoids in the leaf foliage of 4-week-old Gossypium hirsutum plants. Terpenoid levels increased successively from control to mechanical damage, herbivory, and jasmonic acid treatments, with E-β-ocimene and heliocide H1 and H4 showing the highest increases, up to 15-fold. Herbivory or mechanical damage to older leaves led to terpenoid increases in younger leaves. Leaf-by-leaf analysis of terpenes and gland density revealed that higher levels of terpenoids were achieved by two mechanisms: (1) increased filling of existing glands with terpenoids and (2) the production of additional glands, which were found to be dependent on damage intensity. As the relative response of individual terpenoids did not differ substantially among herbivore, mechanical damage, and jasmonic acid treatments, the induction of terpenoids in cotton foliage appears to represent a non-specific wound response mediated by jasmonic acid

Immature Cryopreserved Ovary Restores Puberty and Fertility in Mice without Alteration of Epigenetic Marks

BACKGROUND: Progress in oncology could improve survival rate in children, but would probably lead to impaired fertility and puberty. In pre-pubertal girls, the only therapeutic option is the cryopreservation of one ovary. Three births have been reported after reimplantation of cryopreserved mature ovary. Conversely, reimplantation of ovary preserved before puberty (defined as immature ovary) has never been performed in humans. METHODOLOGY/PRINCIPAL FINDINGS: In order to analyze ovarian function, we performed transplantation using fresh or cryopreserved immature grafts in pre-pubertal or adult mice. Puberty as well as cyclic hormonal activity was restored. All follicle populations were present although a significant reduction in follicle density was observed with or without cryopreservation. Although fertility was restored, the graft is of limited life span. Because ex vivo ovary manipulation and cryopreservation procedure, the status of genomic imprinting was investigated. Methylation status of the H19 and Lit1 Imprinting Control Regions in kidney, muscle and tongue of offsprings from grafted mice does not show significant alteration when compared to those of unoperated mice. CONCLUSIONS/SIGNIFICANCE: These results demonstrate that immature ovarian grafting can restore spontaneous puberty and fertility. However, these data suggest that follicle depletion leads to premature ovarian failure. This study addresses the very important epigenetics issue, and provides valuable information to the study of ovarian transplantation suggesting that these procedures do not perturb normal epigenetics marks. These results are highly relevant to the reimplantation question of immature cortex in women

Survival and development of Campoletis chlorideae on various insect and crop hosts: implications for Bt-transgenic crops

The parasitic wasp, Campoletis chlorideae is an important larval parasitoid of Helicoverpa armigera a serious pest of cotton, grain legumes and cereals. Large-scale deployment of Bt-transgenic crops with resistance to H. armigera may have potential consequences for the development and survival of C. chlorideae. Therefore, we studied the tritrophic interactions of C. chlorideae involving eight insect host species and six host crops under laboratory conditions. The recovery of H. armigera larvae following release was greater on pigeonpea and chickpea when compared with cotton, groundnut and pearl millet. The parasitism by C. chlorideae females was least with reduction in cocoon formation and adult emergence on H. armigera larvae released on chickpea. Host insects also had significant effect on the development and survival of C. chlorideae. The larval period of C. chlorideae was prolonged by 2-3 days on Spodoptera exigua, Mythimna separata and Achaea janata when compared with H. armigera, Helicoverpa assulta and Spodoptera litura. Maximum cocoon formation and adult emergence were recorded on H. armigera (82.4% and 70.5%, respectively) than on other insect hosts. These studies have important implications on development and survival of C. chlorideae on alternate insect hosts on non-transgenic crop plants, when there is paucity of H. armigera larvae on transgenic crops expressing Bt-toxins