Chromium vI induced cytoskeletal damage and cell death in isolated hepatocytes

Cr(VI) is a known human carcinogen. Although it has been investigated widely, the mechanism(s) of its action is/are not fully understood. The aim of this study was to evaluate Cr(VI)-induced damage to the cell cytoskeleton and the mode of cell death in primary cultures of hepatocytes. Exposure of the cultured cells (10(5)/cm(2)) to 1 and 5 microM Cr(VI) for 24 h resulted in loss of the cell cytoskeleton, and this was accompanied by membrane blebbing and shrinking of the cell. Staining of the cells with annexin V and propidium iodide showed that Cr(VI) induces apoptosis at low concentrations (5 microM), whereas at higher concentrations (25 microM) it induces necrosis. This study shows that Cr(VI) causes damage to the cell cytoskeleton, and induces apoptosis at low concentrations. However, the importance of necrosis and apoptosis in vivo, and the effects of longer exposure times, which simulate environmental and occupational exposure to Cr(VI), remain to be investigated

Talking about breastfeeding - emotion, context and 'good' mothering

The benefits of breastfeeding are now recognised and promoted by governments and healthcare services internationally (WHO 2007),with feeding regarded as a significant part of the maternal role: in the words of the World Health Organization: ‘no gift is more precious than breastfeeding’. The idea that breastfeeding can be a ‘gift’ signifies the increasing, heavy cultural and emotional load of feeding for mothers. Feeding practices can be used to differentiate ‘good’ and ‘bad’ mothers, ‘high’ or ‘low’ social status and can also be associated with feelings of intimacy, estrangement, guilt, joy, failure or success. In this article we discuss the findings from the Open University's ‘Becoming aMother’ study (www.open.ac.uk/socialsciences/ identities/findings/Hollway.pdf) in the light of these wider issues and current policy initiatives

Thermocouple Embedding for the Production of a Substrate for Rapid Manufacturing

Mechanical Engineerin

Drug policy in Australia: the supervised injecting facilities debate

Many theories have been developed to explain the influence of different groups in policy making. Some focus on public opinion, claiming that especially in issues of high salience, public opinion will play a decisive role in determining policy directions. Other theories claim interest groups (including professionals) and politicians are more critical in shaping the agenda and the solutions which are seen as viable in addressing a particular problem. The supervised injecting facilities debate in Australia, which reached its peak between 1999 and 2001 when the ACT, Victoria and NSW attempted to establish such facilities, was one characterised by a high degree of conflict between groups with differing moral and political beliefs about drug use. Case studies of each jurisdiction were assembled to delineate the impact of these different groups on the eventual failure to establish trials in ACT and Victoria, compared to success in NSW. The results indicate public opinion was sharply divided on the issue, therefore cannot be considered a major determinant of public policy in this area. Politicians however, because of their ability to block the legislation and funding needed to establish the trials, undoubtedly had the greatest influence in policy making

Go home? the politics of immigration controversies

"The 2013 Go Home vans marked a turning point in government-sponsored communication designed to demonstrate control and toughness on immigration. In this study, the authors explore the effects of this toughness: on policy, public debate, pro-migrant and anti-racist activism, and on the everyday lives of people in Britain. Bringing together an authorial team of eight respected social researchers, alongside the voices of community organisations, policy makers, migrants and citizens, and with an afterword by journalist Kiri Kankhwende, this is an important intervention in one of the most heated social issues of our time.

Parametric programming technique for global optimization of wastewater treatment systems

This paper presents a parametric programming technique for the optimal design of industrial wastewater treatment networks (WTN) featuring multiple contaminants. Inspired in scientific notation and powers of ten, the proposed approach avoids the non-convex bilinear terms through a piecewise decomposition scheme that combines the generation of artificial flowrate variables with a multi-parameterization of the outlet concentration variables. The general non-linear problem (NLP) formulation is replaced by a mixed-integer linear programming (MILP) model that is able to generate near optimal solutions, fast. The performance of the new approach is compared to that of global optimization solver BARON through the solution a few test cases

Optimization of anti-proliferative activity using a screening approach with a series of bis-heterocyclic G-quadruplex ligands

Using a phenotypic screening and SAR optimization approach, a phenyl-bis-oxazole derivative has been identified with anti-proliferative activity, optimized with the use of a panel of cancer cell lines. The lead compound was synthesized by means of a short and effective two-step synthesis using Pd-catalyzed direct arylation. The compound stabilizes several quadruplex DNA sequences including a human telomeric DNA and one from the promoter of the HSP90 gene, although the structure–activity relationships of the series are not obviously related to the quadruplex binding

Learning To Be Affected: Social suffering and total pain at life’s borders.

The practice of Live Sociology in situations of pain and suffering is the author’s focus. An outline of the challenges of understanding pain is followed by a discussion of Bourdieu’s ‘social suffering’ (1999) and the palliative care philosophy of ‘total pain’. Using examples from qualitative research on disadvantaged dying migrants in the UK, attention is given to the methods that are improvised by dying people and care practitioners in attempts to bridge intersubjective divides, where the causes and routes of pain can be ontologically and temporally indeterminate and/or withdrawn. The paper contends that these latter phenomena are the incitement for the inventive bridging and performative work of care and Live Sociological methods, both of which are concerned with opposing suffering. Drawing from the ontology of total pain, I highlight the importance of (i) an engagement with a range of materials out of which attempts at intersubjective bridging can be produced, and which exceed the social, the material, and the temporally linear; and (ii) an empirical sensibility that is hospitable to the inaccessible and non-relational

Can the quality of social research on ethnicity be improved through the introduction of guidance? Findings from a research commissioning pilot exercise

As the volume of UK social research addressing ethnicity grows, so too do concerns regarding the ethical and scientific rigour of this research domain and its potential to do more harm than good. The establishment of standards and principles and the introduction of guidance documents at critical points within the research cycle might be one way to enhance the quality of such research. This article reports the findings from the piloting of a guidance document within the research commissioning process of a major funder of UK social research. The guidance document was positively received by researchers, the majority of whom reported it to be comprehensible, relevant and potentially useful in improving the quality of research proposals. However, a review of the submitted proposals suggested the guidance had had little impact on practice. While guidance may have a role to play, it will need to be strongly promoted by commissioners and other gatekeepers. Findings also suggest the possibility that guidance may discourage some researchers from engaging with ethnicity if it raises problems without solutions; highlighting the need for complementary investments in research capacity development in this area

Lung involvement at presentation predicts disease activity and permanent organ damage at 6, 12 and 24 months follow - up in ANCA - associated vasculitis.

BACKGROUND: Antineutrophilic cytoplasmic antibody (ANCA)-associated vasculitis (AAV) may present with pulmonary involvement ranging from mild to life-threatening disease such as diffuse alveolar hemorrhage. There is a paucity of information regarding morbidity outcomes for AAV subjects presenting with lung involvement. This study determines the relationship between disease activity and damage in these subjects using the Birmingham Vasculitis Activity Score v 3 (BVAS 3) and Vasculitis Damage Index (VDI) respectively. RESULTS: 151 patients with AAV were included with 59 presenting initially with pulmonary involvement. The initial BVAS scores recorded at time of diagnosis were positively correlated with the final VDI scores at 24 months (p \u3c 0.0001, rs = 0.5871). No differences between BVAS and VDI scores were seen for both groups, however in the lung-involvement group only, BVAS scores were significantly higher at 6, 12 and 24 months whilst the VDI scores were significantly higher at 12 and 24 months. Subjects presenting with pulmonary involvement had an increased likelihood for cardiovascular (OR 1.31, 95% CI 0.89, 1.54; p = 0.032) and renal (OR 1.32, 95% CI 1.22, 1.39; p = 0.005) involvement. Subjects presenting with lung involvement with granulomatosis with polyangiitis and microscopic polyangiitis had 24-month VDI scores that were significantly higher (p = 0.027, p = 0.045), and more likely to develop pulmonary fibrosis (OR 1.79, 95% CI 1.48, 2.12; p \u3c 0.001). CONCLUSION: AAV subjects with lung involvement at presentation had a higher disease activity and damage scores at 6, 12 and 24 months follow-up representing a considerable burden of disease despite improvement in overall survival due to the introduction of immunosuppressive therapy