4 research outputs found
Antioxidant and Cytotoxic Activities and Phytochemical Analysis of Euphorbia wallichii Root Extract and its Fractions
Abstract Euphorbia wallichii a perennial herb growing mainly in Himalayas has been widely used in folk medicines for its medicinal properties. In the present study, the crude methanolic root extract (CME) and its fractions; n-Hexane Fraction (NHF), n-Butanol Fraction (NBF), Chloroform Fraction (CHF), Ethyl acetate Fraction (EAF) and Aqueous Fraction (AQF) of this plant specie were investigated for antioxidant and cytotoxic activities and phytochemical analysis. Antioxidant activity was determined by using 2,2-diphenyl-1-picryl-hydrazyl free radical (DPPH) and DNA protection assay performed on pBR322 plasmid DNA. In both these assays, promising results were obtained for CME as well as other fractions. The IC 50 values for DPPH assay were in a range of 7.89 to 63.35 ”g/ml in which EAF showed the best antioxidant potential and almost all the tested samples showed certain level of DNA protection. The cytotoxic activity was assessed by using Sulforhodamine B (SRB) assay on human cell lines; H157 (Lung Carcinoma) and HT144 (Malignant Melanoma). The IC 50 values of the tested samples ranged from 0.18 to 1.4 mg/mL against H157 cell line whereas against HT144 cell line the IC 50 values ranged from 0.46 to 17.88 mg/mL with NBF fraction showing maximum potential for both. Furthermore, the phytochemical analysis of CME and its fractions showed the presences of flavonoids, saponins, tannins, terpenoides and cardiac glycosides with varying concentrations
Novel Strategy of Lactide Polymerization Leading to Stereocomplex Polylactide Nanoparticles Using Supercritical Fluid Technology
The enantiomeric crystallization
of polylactides has removed the
limitations of innate poor thermal and mechanical properties of the
homopolymers. The supercritical fluid technology is an emerging panoramic
version of biomedical polymer synthesis and has proven to be a domineering
substitute to toxic organic solvents. Herein, we report an intriguing,
efficient and a novel polymerization process using supercritical dimethyl
ether (sc-DME) for preparation of polylactides leading to the stereocomplex
polylactide (s-PLA) nanoparticles. The process has generated high
molecular weight homopolymers (Mn â„ 200âŻ000 g mol<sup>â1</sup>) starting from monomers which ultimately crystallized
to a dry powder of s-PLA nanoparticles. The optimum processing parameters
are d/l-lactide polymerization using sc-DME at 130
°C, 400 bar for 5 h with a 30% monomer concentration, keeping
the ratio [monomer]:[tinÂ(II)Â2-ethylhexanoate]:[1-dodecanol] as 3000:1:1
while the stereocomplexation as sc-DME at 70 °C, 350 bar for
2 h. We have investigated the effects of monomer concentration, molecular
weights of homopolymers, times, temperatures, and pressures on the
degree of stereocomplexation. The degree of s-PLA was analyzed by
DSC and XRD. The s-PLA has improved melting point and thermal degradation
than homopolymers. The Youngâs modulus of s-PLA increased to
1.4 GPa with tensile strength (âŒ43 MPa) higher than homopolymers
(âŒ13 MPa) with 3.2% elongation at break. The dry s-PLA powder
shows a diversity of particle size ranging from 30 to 600 nm analyzed
by SEM. The s-PLA finds potential applications in polymer nanofabrication,
biomedical stents and encapsulation, melt-blending, solution casting,
and molding