An in-vivo pilot study into the effects of FDG-mNP in cancer in mice

Purpose Previously, fluorodeoxy glucose conjugated magnetite nanoparticles (FDG-mNPs) injected into cancer cells in conjunction with the application of magnetic hyperthermia have shown promise in new FDG-mNPs applications. The aim of this study was to determine potential toxic or unwanted effects involving both tumour cells and normal tissue in other organs when FDG-mNPs are administered intravenously or intratumourally in mice. Materials and methods FDG-mNPs were synthesized. A group of six prostate-tumour bearing mice were injected with 23.42 mg/ml FDG-mNPs (intravenous injection, n = 3; intratumoural injection into the prostate tumour, n = 3). Mice were euthanized and histological sampling of tissue was conducted for the prostate tumour, as well as for lungs, lymph nodes, liver, kidneys, spleen, and brain, at 1 hour (n = 2) and 7 days (n = 4) post-injection. A second group of two normal (non-cancerous) mice received the same injection intravenously into the tail vein and were euthanised at 3 and 6 months post-injection, respectively, to investigate if FDG-mNPs remained in organs at those time points. Results In prostate-tumour bearing mice, FDG-mNPs concentrated in the prostate tumour, while relatively small amounts were found in the organs of other tissues, particularly the spleen and the liver; FDG-mNP concentrations decreased over time in all tissues. In normal mice, no detrimental effects were found in either mouse at 3 or 6 months. Conclusion Intravenous or intratumoural FDG-mNPs can be safely administered for effective cancer cell destruction. Further research on the clinical utility of FDG-mNPs will be conducted by applying hyperthermia in conjunction with FDG-mNPs in mice

A novel theranostic nanobioconjugate: I-125/131 labeled phenylalanine conjugated boron nitride nanotubes

WOS: 000398027800022Here we report the synthesis of boron nitride nanotubes (BNNTs) via a chemical vapor deposition method, as potential agents for boron neutron capture therapy. BNNTs were functionalized with PAMAM[G-2] dendrimer and then, conjugated with L-Phe using EDC/NHS. After that, BNNTs were radiolabeled with I-125/131, which are commonly used for both therapy and diagnosis in clinical and pre-clinical studies. BNNTs were radiolabeled with a maximum yield with I-125/131 in compared with 4-borono-L-phenyalanine which is currently used as a commercial drug. Radiolabeling parameters were optimized with thin layer radiochromatography and high performance liquid radiochromatography. BNNTs are promising nanobioconjugates as new theranostic agents.Ege University Scientific Research FundEge University [2015 NBE 002]The authors thank to the Ege University Scientific Research Fund for the financial support through the project number 2015 NBE 002. Associate Professor Arzu Turkler Ege and Associate Professor Mehmet Ayvacikli from Celal Bayar University are acknowledged for their valuable helps during the synthesis step of BNNTs. Dr Hasan Demiroglu from Celal Bayar University is acknowledged for his helps during FTIR measurements

Zr-89 Labeled Fe3O4@TiO2 Nanoparticles: in Vitro Afffinities with Breast and Prostate Cancer Cells

UNAK, PERIHAN/0000-0002-5464-2987; tekin, volkan/0000-0002-0264-3814WOS: 000517085900001In this study, Fe3O4@TiO2 nanoparticles were synthesized as a new Positron Emission Tomography/Magnetic Resonance Imaging (PET/MRI) hybrid imaging agent and radiolabeled with Zr-89. in addition, Fe3O4 nanoparticles were synthesized and radiolabeled with Zr-89. Df-Bz-NCS was used as bifunctional ligand. the nanoconjugates were characterized with transmission electron microscopy, scanning electron microscopy, and dynamic light scattering. Radiolabeling yields were 100%. Breast and prostate cancer cell affinities and cytotoxicity were determined using in vitro cell culture assays. the results demonstrated that Fe3O4@TiO2 nanoparticles are promising for PET/MR imaging. Finally, unlike Fe3O4 nanoparticles, Fe3O4@TiO2 nanoparticles showed a fluorescence spectrum at an excitation wavelength of 250 nm and an emission wavelength of 314 nm. Therefore, in addition to bearing the magnetic properties of Fe3O4 nanoparticles, Fe3O4@TiO2 nanoparticles display fluorescence emission. This provides them with photodynamic therapy potential. Therefore multimodal treatment was performed with the combination of PDT and RT by using human prostate cancer cell line (PC3). the development of Zr-89-Df-Bz-NCS-Fe3O4@TiO2 nanoparticles as a new multifunctional PET/MRI agent with photodynamic therapy and hyperthermia therapeutic ability would be very useful.NIH/NCI Cancer CenterUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Cancer Institute (NCI) [P30 CA008748]; Lymph Node Imaging Agents; TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [2140025]NIH/NCI Cancer Center, Grant/Award Number: P30 CA008748; Lymph Node Imaging Agents; TUBITAK, Grant/Award Number: 214002

Examination of the Association Between 3,4-Divanillyltetrahydrofuran Lignan (Urtica dioica Origin) and Prostate Cancer Cells by I-131 Radiolabeling

WOS: 000586257000001PubMed: 32453606Background: Prostate cancer is the most common type of cancer for men in many countries. One of the various prostate cancer therapy methods is hormone therapy, and explaining the association between androgen hormones and prostate cancer is a critical role for successful prostate cancer treatment. Materials and Methods: in the current study, the behavior of 3,4- divanillyltetrahydrofuran (DTH) was examined against prostate cancer cells, which have androgen sensitivity differences [LNCaP (+), PC3 (-)]. For this aim, DTH was obtained by extraction of Urtica dioica roots. the molecular structure of isolated compound was confirmed as DTH by liquid chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy analyses. To evaluate the association of androgen sensitivity, DTH was radiolabeled with I-131, and cell uptake assay was performed by using I-131-radiolabeled DTH. Also, cytotoxicity (WST-1) assay of DTH was performed against LNCaP and PC3 cells to determinate the toxic effects of DTH on different androgen mechanisms. Results: the results of assays on cells have shown that DTH lignan behaves different like being more toxic to LNCaP cells than PC3 cells, depending on androgen sensitivity. Conclusion: the results may contribute both the research topics of phytolignan prostate cancer and androgen-sensitive prostate cancer

Isolation of resveratrol from peanut sprouts, radioiodination and investigation of its bioactivity on neuroblastoma cell lines

KARATAY, Kadriye Busra/0000-0002-2811-4689WOS: 000536433500003Recently, natural antioxidant substances have been purified in a significant increasing incline from different plants for diagnosis and treatment options. in the current study, Resveratrol (RES) was isolated and radioiodinated with iodine-131 ([I-131]iodo-RES). Cell culture studies were conducted on neuroblastoma cells (SY-SH5Y and SK-N-AS) to investigate the bioavailability of [I-131]iodo-RES. the radioiodination yield of RES was 98.81 +/- 0.37% (n = 6). Uptake values up to 25% were observed notably on SK-N-AS cells until 24 h. Briefly; the current study will contribute to the development of novel radiolabeled plant origin agents for the imaging of neuroblastoma cells.Ege University Scientific Research ProjectsEge University; Ege University/TURKEYEge University [FYL-2018-20081]Present study was supported by Ege University Scientific Research Projects, Ege University/TURKEY (Project Number: FYL-2018-20081). Authors thank to Mr. Resat YILDIZ for sending the seeds from "Osmaniye Oil Seeds Research Institute", Osmaniye/TURKEY. Also, authors thank to Dr. Volkan TEKIN and Msc. Cansu KAYAS for their technical help and thank to Mr. Ali Rza GULMEZ for support in growing peanut sprouts