AN ETHNOBOTANICAL STUDY IN CATALCA (EUROPEAN PART OF ISTANBUL) II

In this study, sixty-eight flowering plant species used as medicinal treatment in Catalca (European part of Istanbul) are reported. Among them fifty-eight species are wild and the rest ten species are cultivated plants. The plants in this research are mostly used for treatment of stomach and kidney ailments, cough, diabetes, inflammation and rheumatism. Data presented are based on interviews of local people and materials collected in the area surveyed. Researchers interviewed local people and all the specimens collected together with them were identified and prepared voucher specimens were deposited in the Herbarium of the Faculty of Pharmacy, Istanbul University (ISTE)

EVALUATION OF ANTIMICROBIAL AND CYTOTOXIC EFFECTS OF FOUR TURKISH SPECIES OF ERYNGIUM L.

The antimicrobial and cytotoxic effects of various endemic and non-endemic Eryngium species from Turkey were investigated. Nine endemic and two non-endemic Eryngium species were analyzed using a microdilution assay for their antibacterial and antifungal activities. Cytotoxic activities of E. pseudothoriifolium, E. thorifolium, E. davisii, and E. falcatum, on prostate carcinoma and endometrial cancer cells were analyzed by cytotoxic activity assay. It was observed that 16 out of 22 extracts (aerial or root part) showed antibacterial activities. Fifteen out of 22 extracts showed antifungal activity with the lowest MIC (156 mg/l) value against Candida albicans ATCC 10231 and C. tropicalis ATCC 750. Aerial parts of E. pseudothoriifolium, E. thorifolium, E. davisii, and E. falcatum exhibited cytotoxic effects on endometrial cancer cells. This evaluation of tested endemic Eryngium species' antimicrobial and cytotoxic activities is the first of its kind

Mericarp morphology of the genus Origanum (Lamiaceae) and its taxonomic, significance in Turkey

Origanum is a well-known genus that is widely distributed and comprises many hybrids and endemic species in Turkey. Some morphological and molecular studies in the literature have aimed to clarify infrageneric or sectional classification of the genus. However, studies regarding the shape, size, and color of mericarps of Origanum taxa remain limited. The mericarp micromorphology of 30 taxa, including 7 hybrids of the genus Origanum, were evaluated herein using both stereo microscopy and scanning electron microscopy (SEM) to determine some useful features for taxonomical classification of the genus. The shapes of mericarps are usually ellipsoid, rarely ellipsoid-oblong, obovoid, broadly obovoid, while the color of mericarps vary from shades of brown to reddish. A significant variation was observed in the surface ornamentation of mericarps, which was mainly useful at the sectional and species level. Also determined were 2 main types: I and II, and 5 subtypes: pusticulate, reticulate-ruminate, reticulate-foveate, ruminate, and foveate. The micromorphological characters of mericarps, in addition to their morphological features, will contribute taxonomically to the classification of the sections of the genus Origanum and separation of the species within the sections. In addition, these characters will be useful to understand the effect of hybridization on variations in the micromorphological characters. This was the first detailed study on the mericarp micromorphology of the genus Origanum and its taxonomic implications

In vitro antioxidant activity, cyclooxygenase-2, thioredoxin reductase inhibition and DNA protection properties of Teucrium sandrasicum L.

© 2015 Elsevier B.V.The aerial parts of Teucrium sandrasicum L. (Lamiaceae), an endemic species of the Turkish flora, are widely used in the daily diet. The plant is also valued as a traditional medicine and is used for diuretic, diaphoretic, tonic, antipyretic, antidiabetic, antispasmodic, and cholagogic purposes. This study investigated a methanolic extract of aerial parts of T. sandrasicum for its antioxidant and anti-inflammatory effects, inhibition of thioredoxin reductase, and protection against DNA damage. The extract showed inhibitory activity against FeCl3/ascorbic acid induced phosphatidylcholine liposome oxidation (EC50=1.49±0.03mg/mL), quenched stable DPPH (EC50=1.05±0.03mg/mL), superoxide anion (EC50=1.20±0.06mg/mL), and ABTS cation radicals (EC50=1.73±0.01mg/mL). The extract also acted as a reductant, as determined by the FRAP value (2.66±0.21mM/L Fe2+), indicating its potential as an antioxidant. The T. sandrasicum species was determined to be a rich source of flavonoids (104.39±1.37mg/g), which are likely to serve as antioxidant, anti-inflammatory, and cancer chemoprevention agents. The extract was strongly inhibitory against both cyclooxygenase-2 (COX-2) (EC50=1.18±0.03mg/mL) and thioredoxin reductase (0.90±0.02mg/mL), and it significantly and dose-dependently decreased the amount of nicked DNA, as well as increased the natural form of DNA

Analysis of cytotoxic T lymphocyte antigen-4 (CTLA-4) exon 1 polymorphism in patients with type 1 diabetes mellitus in a Turkish population

Recent studies have described linkage and association between cytotoxic T-lymphocyte antigen-4 (CTLA-4) gene polymorphism and type 1 diabetes mellitus (DM1) in some ethnic populations, but not others. This finding suggests that CTLA-4 gene association with DM1 may be influenced by the racial composition of the population. Thus, it is important to study the polymorphism of the CTLA-4 gene in different ethnic groups. In this case-control association study, the CTLA-4 gene exon 1 A/G polymorphism was analyzed in 48 children with DM1 and 80 healthy controls using polymerase chain reaction-restriction fragment length polymorphism analysis. The possible interaction of the CTLA-4 gene polymorphism with the presence of established genetic markers (HLA-DR genotyping) was also evaluated in 29 patients, The results of the present study do not suggest an association of the known polymorphism in exon 1 of the CTLA-4 gene with DM1 in this Turkish population, and G-allele containing CTLA-4 genotypes were not preferentially associated with age at clinical presentation or with presence of other genetic (HLA-DR3 or -DR4) markers of DM1

A composite neural network model for perseveration and distractibility in the Wisconsin card sorting test

A composite artificial neural network model is proposed to simulate the performance of the Wisconsin Card Sorting Test. The Wisconsin Card Sorting Test is a test of executive functions where prefrontal deficits are matched to some quantitative measures such as percentage of perseverative errors and number of failures to maintain set. In this work, the proposed model is used to simulate the performances of healthy subjects and patients with prefrontal involvement particularly on these measures. The model is designed in such a way that one of the subsystems, namely, the Hopfield network, serves as the working memory and the other, the Hamming block, as the hypothesis generator. The results show that the proposed relatively simple model is capable of simulating the wide range of the performances of both normal subjects and prefrontal patients on the Wisconsin Card Sorting Test. While lowering the Hamming distance in the Hamming block gave rise to progressively more perseverative responses, changing the threshold vector of the Hopfield network resulted in more set maintenance failures. The former manipulation disrupts the abstraction or mental flexibility and the latter sustained attention or perseverance both of which are the major functions of the prefrontal system. (c) 2005 Elsevier Ltd. All rights reserved

Biological evaluation of stachys iberica subsp. stenostachya (boiss.) rech.f. And scutellaria orientalis subsp. sosnowskyi (takht.) fed. growing in eastern anatolia

© 2021 International Journal of Secondary Metabolite. All rights reserved.Lamiaceae is one of the largest families in the plant kingdom, including the genus Stachys and Scutellaria, which are used in many folk medicines throughout the world for the prevention and also the treatment of several disorders. In vitro biological potential of Stachys iberica subsp. stenostachya and Scutellaria orientalis subsp. sosnowskyi were investigated in the current study. The aerial parts of the plants were extracted using different solvents such as n-hexane, chloroform, and methanol. In addition, infusions of each plant were prepared. The antioxidant potential of the samples was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ABTS assays, ferrous ion-chelating, and ferric reducing antioxidant power (FRAP) assays. Anticholinesterase activity of the extracts was also determined. Spectrophotometric analysis was used to assess the total phenolic content of the samples. The antimicrobial activities of samples were determined by minimal inhibitory concentration (MIC) against seven bacteria and three Candida spp. yeast. According to the findings, the infusion demonstrated significant antioxidant properties, whilst the extracts demonstrated high-to-moderate antioxidant effects. The n-hexane extracts showed higher antifungal activity against C.parapsilosis and C.tropicalis. These outcomes suggest that these two species from Turkey could be employed in the manufacture of phytopharmaceuticals

PREDICTION OF LEAF WATER STATUS USING SPECTRAL INDICES FOR YOUNG OLIVE TREES

WOS: 000326911800012It is important to determine the plant water stress before it can reduce the yield or becomes visible. The aim of this study was to investigate the relationship between remotely sensed hyperspectral reflectance indices and leaf water status (LWS) of olive seedlings (Olea europaea L. cv. 'Ayvalik', 'D9', 'D36', 'Erdek Yaglik', 'Frantoio' and 'Gemlik') at different irrigation regime. A pot experiment was conducted in field conditions with 2-years old olive seedlings for the seasons 2011 and 2012. Four levels of water treatment were applied to the pots to bring about different stress conditions; no stress (I-100), mild stress (I-66), severe stress (I-33) and full stress (I-0). Leaf water potential (LWP) and relative water content (RWC) were determined to assess the LWS of the plants. In addition, canopy spectral reflectance was measured with a handheld spectroradiometer and several spectral vegetation indices were calculated using canopy reflectance data. Analysis showed that the irrigation requirement of Frantoio cultivar was the highest when compared to that of other cultivars, while the lowest amount of water was required by Ayvalik cultivar. According to the stepwise multiple linear regression (SMLR) analysis between spectral indices and LWS of olive seedlings, the coefficient of determination (R-2) of model between RWC and Photochemical Reflectance Index (PRI) was 0.70, while it was 0.81 between LWP and PRI, Soil Adjusted Vegetation Index (SAVI), Normalized Difference Vegetation Index (NDVI) and Normalized Pigment Chlorophyll Index (NPCI). Accordingly, it appeared that LWP could be detected more accurately than RWC using spectral indices. Results of this study indicated that the olive plant was very susceptible to water stress and the remotely sensed spectral data could be used to determine RWC and LWP as an indicator of water stress.General Directorate of Agricultural Research and Policy (GDAR)Gida Tarim Ve Hayvancilik BakanligiThis research was funded by the General Directorate of Agricultural Research and Policy (GDAR)

Effects of love glove application on vital signs for COVID‐19 patients in the intensive care unit

Abstract Aim To evaluate the effects of love glove application on vital signs for COVID‐19 patients in the intensive care unit. Design A single‐group pretest‐posttest quasi‐experimental design was used. TREND Statement Checklist was followed during the present study. Methods The study was conducted on 30 intubated/extubated adult patients. The gloves were filled with warm water and air to prevent pressure injuries. Then they were tied together and applied to both hands of the patient for 30 min. The patient's vital signs were recorded before and after the application. A Wilcoxon signed‐rank test was performed. Results It was determined that respiratory rate, systolic blood pressure, diastolic blood pressure and oxygen saturation were significantly affected after the application of the love glove. The application of love gloves is a cheap and non‐pharmacological method with no side effects. Patient or Public Contribution Patients were involved in the design and conduct of this study

Comparative Studies on Essential Oil and Phenolic Content with In Vitro Antioxidant, Anticholinesterase, Antimicrobial Activities of Achillea biebersteinii Afan. and A. millefolium subsp. millefolium Afan. L. Growing in Eastern Turkey

The potential applications of Achillea species in various industries have encouraged the examination of their phytochemical components along with their biological potential. In the present study, phenolic contents and essential oil compositions together with the in vitro biological activities of the aerial parts from Achillea biebersteinii Afan. and Achillea millefolium subsp. millefolium Afan. collected from Turkey were evaluated. Different solvent extracts (n-hexane, chloroform, methanol, water) were prepared and their antimicrobial, anticholinesterase, and antioxidant activities were studied. The LC-MS/MS results revealed the presence of 16 different phenolic compounds, including chlorogenic acid, rutin, quercetin, and luteolin glycosides, in methanolic extracts. According to GC-FID and GC/MS results, the primary components of the oils were identified as 1,8-cineole (32.5%), piperitone (14.4%), and camphor (13.7%) in A. biebersteinii and 1,8-cineole (12.3%) and beta-eudesmol (8.9%) in A. millefolium subsp. millefolium. The infusion and methanolic extracts of both species were found to be rich in their total phenolic content as well as their antioxidant and anticholinesterase activity. In contrast, the n-hexane and chloroform extracts of both species showed strong antimicrobial activity with MIC values ranging from 15 to 2000 mu g/mL. Our findings suggest that the investigated Achillea species could be evaluated as potent natural agents, and further studies into the promising extracts are needed