Separation and characterization of biosurfactant from P.aeruginosa sp1 isolated from oil mill area MIDC, Parbhani (M.S.)

Biosurfactant are an amphiphilic compound that reduces surface and interfacial tensions by accumulating at the interface of immiscible fluids and increase the surface areas of insoluble compounds leading to increased mobility. The extraction of the biosurfactant from the cell free supernatant using the solvent extraction procedure and the quantitative analysis has been discussed. The application of the biosurfactant includes biomedical, cosmetics and bioremediation rhamnolipid biosurfactant produced by Pseudomonas aeruginosa sp showed significant applications in the bioremediation of hydrocarbon in gasoline spilled oil and petroleum oily sludge in this review we discuss the potential roles and applications of biosurfactant, mainly focusing on areas such as food and food related industries, biomedicine and therapeutics

Screening and optimization of biosurfactant producing bacteria from oil mill area MIDC, Parbhani (M.S.)

Biosurfactant are amphiphilic compounds produced on living surfaces, mostly on microbial cell surfaces. They are biodegradable non- toxic and ecofreindly materials. Pseudomonas aeruginosa isolated from oil mill area produced biosurfactant at 370C at pH 7 on YPG medium and mineral salt medium. The biosurfactant production depends on the fermentation conditions, environmental factors and nutrient availability. The extraction of the biosurfactant from the cell free supernatant using the solvent extraction procedure & the qualitative and quantitative analysis has been discussed with appropriates equipment details

Enhance Crawler For Efficiently Harvesting Deep Web Interfaces

Scenario in web is varying quickly and size of web resources is rising, efficiency has become a challenging problem for crawling such data. The hidden web content is the data that cannot be indexed by search engines as they always stay behind searchable web interfaces. The proposed system purposes to develop a framework for focused crawler for efficient gathering hidden web interfaces. Firstly Crawler performs site-based searching for getting center pages with the help of web search tools to avoid from visiting additional number of pages. To get more specific results for a focused crawler, projected crawler ranks websites by giving high priority to more related ones for a given search. Crawler accomplishes fast in-site searching via watching for more relevant links with an adaptive link ranking. Here we have incorporated spell checker for giving correct input and apply reverse searching with incremental site prioritizing for wide-ranging coverage of hidden web sites

Processing of Beach Sand Minerals of Maharastra Coast

In the present paper, an attempt has been to bene-ficiate beach sand minerals of Maharastra coast. For this purpose, beach sand mineral samples were collected from two different coastal places namely; Pirawadi and Kother-wadi area. The samples obtained were subjected for chara-cterization & beneficiation studies. The characterization studies were mainly carried out by mineralogical analyses. The mineralogical analyses indicated 12-15% Ilmenite, 5-7% Magnetite and 65-70% Quartz in Pirawadi sample. Similarly Kotherwadi sample showed the presence of 10-12% Ilmanite along with 40-45% quartz. The studies also indicated the increased heavy mineral concentration in finer size (-75 11) fractions. Further, the beneficiation studies were carried out using Perm roll magnetic separator using different variables such as particle size, magnetic inte-nsity, roll speed, splitter position etc. From the studies it was possible to recover 80-85% magnetic minerals under different conditions

Extraction, purification and analysis of thermal stability of xylose isomerase

Thermostable enzymes are the enzymes which active even at high temperatures, such enzymes are industrially as well as biochemically very important. Xylose isomerase (EC 5.3.1.5) is one such enzyme with suitable commercial applications. It is heat stable and does not require expensive cofactors such as NAD or ATP for activity. The microorganisms producing this enzyme were isolated from hot water spring near ‘Surat’. The organisms were isolated and purified by using different screening methods. The isolated organisms were then subjected to optimum growth conditions for enzyme production. This enzyme was then assayed for its thermal stability at elevated temperatures by using DNSA

Analysis of protease activity of enzyme isolated from compost soil

Bacteria are very good source of enzymes as compared to animal or plant source and even synthetic enzymes. In the present investigation the protease activity has been analyzed. The source of enzyme i.e. protease producers were isolated from compost soil sample viz. collected from the Wanker farm field, Solapur. The protease producers were isolated, screened and grown on a suitable growth medium to obtain maximum production of enzyme. After production the crude enzyme is purified. The purified enzyme is analysed for its keratinolytic activity by using feathers

A comparative clinical study on Tagara-Devadaru Lepa with and without Prachhanna in the management of Indralupta with special reference to Alopecia Areata

Hair disorders causes negative impact towards individual and his/her quality of life. Indralupta is a disease in which hair is lost from some or all areas of the body, usually from scalp. It shows one or more round spot on the scalp . It arises from the vitiation of Tridosha and Rakta. Treatment advised is Siravedhana and topical application of herbal and mineral drugs. To avoid complications of Siravedhana, inthis study Prachhanna was preffered and Tagara-Devadaru chosen as drug for topical application at the site of Indralupta. In this study we had taken 40 Diagnosed patients of Indraluptaand were subjected to clinical trials. They were randomly assigned into two Groups namely Group A and Group B. Group A treated with Tagara-Devadaru Lepa, while subject under Group B treated by Prachhanna along with Tagara-Devadaru Lepa. The treatment modalities of TagaraDevadaru Lepa and Prachhanna with Tagara-Devadaru Lepa are equally efficacious in treating.On comparing the results of Group A and Group B, the conclusion were drawn.Both the methods of treatment are cost effective, easy to prepare and had no adverse effects

A clinical comparative study of Chedana Paschat Arka Pratisaraeeiya Kshara & Agnikarma in the management of Kadara (Corn)

Sushruta Samhita is the earliest known authentic treatise on Ayurveda. In Vedic period also there is a description of Agnikarma and Ksharakarma. In Shalya Tantra common procedure are Agnikarma, Ksharakarma and Jalokavacharna etc. Kadara is one among Kshudra Roga is claimed to be effectively dealt with Agnikarma and Ksharakarma. This is intended to offer instant relief to the patient to evolve a simple and economic management and to evaluate whether the relapse can be prevented with Agnikarma and Ksharakarma.The studies conducted with 40 patients were selected, made into 2 groups of 20 each patients. Group A patients were treated with surgical excision followed by Arka Pratisaraneeya Ksharakarma and Group B patients were treated with surgical excision followed by Agnikarma. Arka Kshara is prepared and applied after excision of lesion, keeping for 100 Shatamatrakala (100 sec), treated with Nimbuka Swarasa. With all aseptic measures the lesion is excised and then Agnikarma using Lohashalaka, Madhu and Sarpi is applied and bandaged. Both procedures are in single sitting, dressing for alternate day and every 15 days follow up till 45 days. After the completion of clinical trial, it was found that Agnikarma procedure there was highly significant results in reducing pain, discomfort, bleeding infections and healing period. Where as in KsharaKarma there is also significant result in reducing pain, discomfort but statically considering average mean Kshara Karma shows comparatively lesser effective than Agnikarma. By the statistical results it can be concluded that Agnikarma has better result when compared to Kshara Karma in the present study

Intussusception due to caecal carcinoma in a young man: unusual cause of presentation a case report

A young 26 year male patient admitted with colicky pain in right iliac fossa with well palpable tender lump. After radiological investigation lump was diagnosed as ileocaecal intussusception. Patient underwent laparoscopy which diagnosed as intussusception due to caecal carcinoma. Laparoscopy again proved to be useful diagnostic tool over imaging techniques in this case. Laparoscopic assisted surgery of right radical hemicolectomy done successfully