Expression of VSTM1-v2 in PBMCs from RA patients correlates with DAS28, CRP and ESR.

<p>The correlations between VSTM1-v2 mRNA expression level and RA disease characteristics were determined by using Spearman test. The correlations between VSTM1-v2 expression level and 28-joints disease activity score (DAS28) (Fig 3A), C-reactive protein (CRP) (Fig 3B), and erythrocyte sedimentation rate (ESR) (Fig 3C) were presented.</p

Correlation of VSTM1-v2 expression level and IL-17A mRNA expression level.

<p>(A) The expression IL-17A mRNA was detected with real time-PCR. Relative IL-17A mRNA was normalized to GAPDH in PBMCs from patients with rheumatoid arthritis (RA; n = 40) and healthy controls (HC; n = 40). Data are shown as scatter plot. <i>P</i> value was determined by Mann-Whitney test. (B) The correlation between VSTM1-v2 mRNA expression level and IL-17A mRNA expression level was determined by using Spearman test. The relation between VSTM1-v2 and IL-17A was presented.</p

Correlations of VSTM1-v2 mRNA expression on PBMCs in RA patients with RF, Anti-CCP and ANA.

<p>The correlations between VSTM1-v2 mRNA expression level and RA disease characteristics were determined by using Spearman test. The correlations between VSTM1-v2 expression level and rheumatoid factor (RF) (Fig 4A), anti-cyclic citrullinated protein antibodies (anti-CCP) (Fig 4B) and antinuclear antibodies (ANA) (Fig 4C) were presented.</p

Increased VSTM1-v2 expression in PBMCs from RA patients.

<p>The expression of VSTM1-v2 mRNA was detected by real time-PCR. Relative VSTM1-v2 mRNA expression was normalized to GAPDH in PBMCs from patients with rheumatoid arthritis (RA; n = 40) and healthy controls (HC; n = 40). Data are shown as scatter plot. <i>P</i> value was determined by Mann-Whitney test.</p

Additional file 1: Table S1. of MicroRNA-488 and -920 regulate the production of proinflammatory cytokines in acute gouty arthritis

Primer sequences used for the amplification of IL-1β 3′UTR. (DOCX 15 kb

Additional file 2: of MicroRNA-488 and -920 regulate the production of proinflammatory cytokines in acute gouty arthritis

IL-1β gene expression in the peripheral white blood cells of patients with acute gouty arthritis (GA, n = 14) and of healthy control subjects (HC, n = 10). IL-1β mRNA expression was detected by quantitative real-time PCR. Each bar shows the mean ± SEM. (DOCX 48 kb

Additional file 2: of MicroRNA-488 and -920 regulate the production of proinflammatory cytokines in acute gouty arthritis

IL-1β gene expression in the peripheral white blood cells of patients with acute gouty arthritis (GA, n = 14) and of healthy control subjects (HC, n = 10). IL-1β mRNA expression was detected by quantitative real-time PCR. Each bar shows the mean ± SEM. (DOCX 48 kb

Additional file 3: of MicroRNA-488 and -920 regulate the production of proinflammatory cytokines in acute gouty arthritis

THP-1 cells were transfected with 50 nM of miRNA mimics or negative control (scrambled) by using Lipofectamine RNAiMAX reagent. Expression of miRNAs was detected by quantitative real-time PCR. Each bar shows the mean ± SEM. (DOCX 31 kb

Correlation between PCT and ASDAS, CRP, ESR and WBC.

<p>Correlation of the serum levels of PCT with ASDAS, CRP, ESR and WBC was evaluated by Spearman correlation test in AS patients (n = 41). PCT was significantly positively correlated with CRP (P = 0.001). No correlation between PCT and ASDAS, ESR and WBC were detected.</p

PCT is highly expressed in GA in comparison to RA, AS and Healthy control.

<p>(A) The comparison of serum PCT in GA (n = 51), RA (n = 37), AS (n = 41) and healthy control (n = 33) were determined by Mann–Whitney U-test. In GA patients PCT was significantly higher level observed than in RA (p = 0.002), AS (p = 0.0007) and healthy control (p<0.0001), whilst no significant differences in between RA and AS were observed. (B) The GA patients were divided into GA (tophi) (n = 29) and GA (none tophi) (n = 22) which indicates GA patients with tophi or not respectively. GA Serum PCT level was compared among these groups.</p