Papel da interleucina-4 e da prostaglandina E2 durante a infecção de camundongos BALB

Submitted by Repositório Arca ([email protected]) on 2019-07-08T18:54:13Z No. of bitstreams: 1 license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2019-07-15T13:36:30Z (GMT) No. of bitstreams: 2 Elisalva Teixeira Guimarães Papel...da 2004.pdf: 31726177 bytes, checksum: 6dfed656e594f39d1e235f8cf9ec60e0 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5)Made available in DSpace on 2019-07-15T13:36:30Z (GMT). No. of bitstreams: 2 Elisalva Teixeira Guimarães Papel...da 2004.pdf: 31726177 bytes, checksum: 6dfed656e594f39d1e235f8cf9ec60e0 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2004CAPES/FIOCRUZUniversidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.O papel de citocinas durante a infecção experimental por L. amazonensis não é tão bem estudado quanto no modelo de infecção por L. major. Neste trabalho foi investigado o papel dos mediadores solúveis IL-4 e PGE2 no curso da infecção de camundongos BALB/c por L. amazonensis. Camundongos BALB/c IL-4 +/+ e IL-4 -/- foram infectados por diferentes inóculos de L. amazonensis e submetidos ao tratamento com indometacina, um inibidor da síntese de PGE2. A produção de IFN-y (no sobrenadante da cultura de células do linfonodo) e os níveis de anticorpos no soro foram determinados por ELlSA. Os níveis de NO sé ricos foram determinados através de seus produtos, o nitrito e o nitrato, pela reação de Griess. Apesar das lesões e da carga parasitária em camundongos BALB/c IL-4 +/+ e IL-4 -/- progredirem de forma similar quando infectados por 5 x 106 L. amazonensis, houve um aumento de esposta Th1 em camundongos IL-4 -/- como indicado pela produção elevada de IFNy e de anticorpos anti-Ieishmania IgG2a neste grupo de animais. A produção de NO foi semelhante nos dois grupos de estudo. No entanto, camundongos IL-4 -/desenvolvem lesões e carga parasitária menores que IL-4 +/+ após a infecção por 05, 104 ou 103 parasitos. Camundongos IL-4 -/- produzem cerca de 100 vezes mais NO que IL-4 +/+ após a infecção por 104 L. amazonensis. Após o tratamento com indometacina, há uma diminuição das lesões e da carga parasitária em camundongos BALB/c IL-4 +/+. Em contrapartida, apesar haver uma redução significativa no tamanho das lesões de camundongos BALB/c IL-4 -/- tratados com esta droga, nenhuma diferença na carga parasitária foi observada. As lesões dos grupos tratados com indometacina apresentavam macrófagos não vacuolados e pequenas ou ausentes áreas de necrose. A PGE2 e a IL-4 são fatores associados à susceptibilidade durante a infecção por L. amazonensis.The role of cytokines in Leishmania amazonensis experimental infection has not been well studied as in the L. major infection model. Here we investigated the role of interleukin (IL)-4 and PGE2 in L. amazonensis infection of susceptible BALB/c mice. [METHODS] Interleukin (IL)-4 deficient (IL-4-/-) or wild- type (IL-4+/+) BALB/c mice were infected with L. amazonensis promastigotes and submitted to treatment with indomethacin, an inhibitor of PGE2 synthesis. Cytokine (in lymph node cell culture supernatants) and serum anti-leishmania antibody levels were determined by ELISA. Nitric oxide production was estimated by the assessment of the NO2 and NO3 levels in sera using the Griess reaction. [RESULTS] Lesions and parasitic load were similar in IL-4 +/+ and IL-4-/- mice upon infection with 5 x 10 parasites, despite the higher production of interferon (IFN)-y and lgG2a in IL-4 -/- mice compared to IL-4 +/+ mice. The NO levels were similar in both groups. However, IL-4 -/- mice developed smaller lesions and parasitic loads upon infection with 10,10 or 10^ parasites, compared to IL-4 +/+ mice. The NO production was 100 times higher in sera from IL-4 -/- than in IL-4 +/+ mice upon infection with 10^ L amazonensis. IL-4 +/+ mice treated with indomethacin during the first three weeks of infection developed smaller lesions and lower parasitic load when compared to the control group. However, although indomethacin treatment caused a significant reduction in lesion size of IL-4 -/- mice, no difference in the parasitic load was observed. The lesions of indomethacin tTeated groups contained most macrophages without vacuoles and small or absent necrotic areas. [CONCLUSION] These results indicate that IL-4 and PGE2 are susceptibility factors to L. amazonensis infection

Role of interleukin-4 and prostaglandin E2 in Leishmania amazonensis infection of BALB/c mice.

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-05-28T16:49:50Z No. of bitstreams: 1 Guimaraes ET Role of interleukin....pdf: 2518206 bytes, checksum: 5aa9ae54662af73cda95cec5f944510a (MD5)Made available in DSpace on 2014-05-28T16:49:50Z (GMT). No. of bitstreams: 1 Guimaraes ET Role of interleukin....pdf: 2518206 bytes, checksum: 5aa9ae54662af73cda95cec5f944510a (MD5) Previous issue date: 2006Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Instituto de Ciências Biológicas. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilThe role of cytokines in Leishmania amazonensis experimental infection has not been as well studied as in Leishmania major infection model. Here we investigated the role of interleukin (IL)-4 and PGE(2) in L. amazonensis infection of susceptible BALB/c mice. IL-4 deficient (-/-) or wild-type (+/+) BALB/c mice were infected with different inocula of L. amazonensis. Two weeks after infection with 5x10(6) promastigotes/footpad, the production of interferon (IFN)-gamma upon L. amazonensis antigen stimulation was significantly higher in lymph node cell cultures of IL-4-/- mice than in IL-4+/+ mice. The levels of anti-leishmania IgG2a antibodies were also significantly higher in serum from IL-4-/- mice. In contrast, the levels of IgG1 antibodies were increased in IL-4+/+ mice and almost undetectable in IL-4-/- mice. Despite the increased Th1 response, lesions of IL-4-/- BALB/c mice progressed similarly to those of IL-4+/+ mice upon infection with the 5x10(6) inoculum. However, IL-4-/- mice developed smaller lesions upon infection with 10(5), 10(4) or 10(3) parasites than IL-4+/+ mice. The resistance of IL-4-/- correlated with higher Th1 response, compared to IL-4+/+ upon infection with 10(4)L. amazonensis. IL-4+/+ mice treated with indomethacin, an inhibitor of PGE(2) synthesis, during the first 3weeks of infection developed smaller lesions and lower parasitic load when compared to the control group. The lesions of indomethacin-treated groups contained mostly macrophages without vacuoles and small or absent necrotic areas. These results indicate that IL-4 and PGE(2) are susceptibility factors to L. amazonensis infection

Antiparasitic evaluation of betulinic acid derivatives reveals effective and selective anti-Trypanosoma cruzi inhibitors

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2016-12-12T12:19:04Z No. of bitstreams: 1 Meira CS Antiparasitic evaluation....pdf: 1441373 bytes, checksum: 57e6aa961c1ae67aa08d5af7ce78c3fc (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2016-12-12T12:43:14Z (GMT) No. of bitstreams: 1 Meira CS Antiparasitic evaluation....pdf: 1441373 bytes, checksum: 57e6aa961c1ae67aa08d5af7ce78c3fc (MD5)Made available in DSpace on 2016-12-12T12:43:14Z (GMT). No. of bitstreams: 1 Meira CS Antiparasitic evaluation....pdf: 1441373 bytes, checksum: 57e6aa961c1ae67aa08d5af7ce78c3fc (MD5) Previous issue date: 2016-07CNPq (grant number 562655/2010-7), PRONEX (grant number 0002/2014), FAPESB (grant number 0042/2013) and FINEP (grant number 01.04.0320-00).Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Paraíba. Laborat orio de Tecnologia Farmacêutica. João Pessoa, PB, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade do Estado da Bahia. Departamento de Ciências da Vida. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilBetulinic acid is a pentacyclic triterpenoid with several biological properties already described, including antiparasitic activity. Here, the anti-Trypanosoma cruzi activity of betulinic acid and its semi-synthetic amide derivatives (BA1-BA8) was investigated. The anti-Trypanosoma cruzi activity and selectivity were enhanced in semi-synthetic derivatives, specially on derivatives BA5, BA6 and BA8. To understand the mechanism of action underlying betulinic acid anti-T. cruzi activity, we investigated ultrastructural changes by electron microscopy. Ultrastructural studies showed that trypomastigotes incubated with BA5 had membrane blebling, flagella retraction, atypical cytoplasmic vacuoles and Golgi cisternae dilatation. Flow cytometry analysis showed that parasite death is mainly caused by necrosis. Treatment with derivatives BA5, BA6 or BA8 reduced the invasion process, as well as intracellular parasite development in host cells, with a potency and selectivity similar to that observed in benznidazole-treated cells. More importantly, the combination of BA5 and benznidazole revealed synergistic effects on trypomastigote and amastigote forms of T. cruzi. In conclusion, we demonstrated that BA5 compound is an effective and selective anti-T. cruzi agent

Anti-Trypanosoma cruzi activity of nicotinamide

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-08-11T19:30:51Z No. of bitstreams: 1 Soares MB Anti-Trypanosoma....pdf: 1207763 bytes, checksum: 69f837370e5c46f7e1843460c2bd4154 (MD5)Made available in DSpace on 2014-08-11T19:30:51Z (GMT). No. of bitstreams: 1 Soares MB Anti-Trypanosoma....pdf: 1207763 bytes, checksum: 69f837370e5c46f7e1843460c2bd4154 (MD5) Previous issue date: 2012Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilLaboratory of Molecular Parasitology, Microbiology. Dpt, Hellenic Pasteur Institute. Athens, GreecePontifícia Universidade Católica do Rio Grande do Sul – PUCRS. Faculdade de Biociências. Instituto Nacional de Ciência e Tecnologia em Tuberculose-CNPq. Laboratório de Bioquímica Estrutural. Porto Alegre, RS, Brasil / Pontifícia Universidade Católica do Rio Grande do Sul. Porto Alegre, RS, BrasilInhibition of Trypanosoma brucei and Leishmania spp. sirtuins has shown promising antiparasitic activity, indicating that these enzymes may be used as targets for drug discovery against trypanosomatid infections. In the present work we carried out a virtual screening focused on the C pocket of Sir2 from Trypanosoma cruzi. Using this approach, the best ligand found was nicotinamide. In vitro tests confirmed the anti-T. cruzi activity of nicotinamide on epimastigote and trypomastigote forms. Moreover, treatment of T. cruzi-infected macrophages with nicotinamide caused a significant reduction in the number of amastigotes. In addition, alterations in the mitochondria and an increase in the vacuolization in the cytoplasm were observed in epimastigotes treated with nicotinamide. Analysis of the complex of Sir2 and nicotinamide revealed the details of the possible ligand–target interaction. Our data reveal a potential use of TcSir2 as a target for anti-T. cruzi drug discovery

Coleta e cultura de células-tronco obtidas da polpa de dentes decíduos: técnica e relato de caso clínico

INTRODUÇÃO: as células-tronco (CT) possuem capacidade de induzir a regeneração tecidual e, portanto, apresentam um potencial terapêutico. Assim como a medula óssea e o cordão umbilical, a polpa dentária é uma das fontes disponíveis de CT. O seu fácil acesso e o fato de os dentes decíduos não serem órgãos vitais, que normalmente são descartados após a esfoliação, provêm um atrativo para testes de segurança e viabilidade terapêutica dessas células. OBJETIVOS: descrever a coleta, o isolamento e o cultivo de CT obtidas da polpa de dentes decíduos, assim como a sua caracterização por meio de citometria de fluxo e da indução da diferenciação em linhagens osteogênica e adipogênica. MÉTODOS: as CT foram obtidas de forma relativamente simples e apresentaram boa capacidade proliferativa, mesmo a partir de pouca quantidade de tecido pulpar. RESULTADOS: a análise por citometria de fluxo confirmou as características de CT mesenquimais, com baixos níveis de expressão dos antígenos CD34 e CD45, que são marcadores de células hematopoiéticas, e altos níveis de expressão dos antígenos CD105, CD166, CD90 e CD73, que são marcadores de CT mesenquimais. A plasticidade das células foi confirmada pela identificação de depósitos de cálcio nas culturas que receberam meio osteogênico, e de acúmulo lipídico intracelular nas culturas que receberam meio adipogênico. CONCLUSÕES: as CT de dentes decíduos têm um potencial promissor de aplicação em regeneração tecidual. Sendo assim, é importante difundir entre os cirurgiões-dentistas o conhecimento sobre a existência e as características dessa fonte de CT, discutindo a técnica utilizada, suas limitações e possíveis indicações

Coleta e cultura de células-tronco obtidas da polpa de dentes decíduos: técnica e relato de caso clínico / Collection and culture of stem cells derived from dental pulp of deciduous teeth: technique and clinical case report

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2012-12-20T17:01:13Z No. of bitstreams: 1 Jesus, Alan Araujo Coleta e cultura....pdf: 447904 bytes, checksum: ee80c238abddd952602fa3780d66cdc7 (MD5)Made available in DSpace on 2012-12-20T17:01:13Z (GMT). No. of bitstreams: 1 Jesus, Alan Araujo Coleta e cultura....pdf: 447904 bytes, checksum: ee80c238abddd952602fa3780d66cdc7 (MD5) Previous issue date: 2011Universidade Estadual de Feira de Santana. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Odontologia. Salvador, BA, BrasilUniversidade Estadual de Feira de Santana. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Board Brasileiro de Ortodontia e Ortopedia Facial. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Monte Tabor. Centro Ítalo Brasileiro de Promoção Sanitária. Salvador, BA, BrasilINTRODUÇÃO: as células-tronco (CT) possuem capacidade de induzir a regeneração tecidual e, portanto, apresentam um potencial terapêutico. Assim como a medula óssea e o cordão umbilical, a polpa dentária é uma das fontes disponíveis de CT. O seu fácil acesso e o fato de os dentes decíduos não serem órgãos vitais, que normalmente são descartados após a esfoliação, provêm um atrativo para testes de segurança e viabilidade terapêutica dessas células. OBJETIVOS: descrever a coleta, o isolamento e o cultivo de CT obtidas da polpa de dentes decíduos, assim como a sua caracterização por meio de citometria de fluxo e da indução da diferenciação em linhagens osteogênica e adipogênica. MÉTODOS: as CT foram obtidas de forma relativamente simples e apresentaram boa capacidade proliferativa, mesmo a partir de pouca quantidade de tecido pulpar. RESULTADOS: a análise por citometria de fluxo confirmou as características de CT mesenquimais, com baixos níveis de expressão dos antígenos CD34 e CD45, que são marcadores de células hematopoiéticas, e altos níveis de expressão dos antígenos CD105, CD166, CD90 e CD73, que são marcadores de CT mesenquimais. A plasticidade das células foi confirmada pela identificação de depósitos de cálcio nas culturas que receberam meio osteogênico, e de acúmulo lipídico intracelular nas culturas que receberam meio adipogênico. CONCLUSÕES: as CT de dentes decíduos têm um potencial promissor de aplicação em regeneração tecidual. Sendo assim, é importante difundir entre os cirurgiões-dentistas o conhecimento sobre a existência e as características dessa fonte de CT, discutindo a técnica utilizada, suas limitações e possíveis indicações.Stem cells (SCs) are capable of inducing tissue regeneration and are, therefore, potentially therapeutic. Similarly to bone marrow and umbilical cords, dental pulp is one of the available sources of SCs. The fact that these cells are easily accessible and that deciduous teeth are not vital organs, and are normally discarded after exfoliation, make them particularly attractive for use in safety and viability tests. OBJECTIVE: To describe the collection, isolation and culture of SCs obtained from the pulp of deciduous teeth as well as their characterization by flow cytometry, and the induction of differentiation into osteogenic and adipogenic lineages. METHODS: SCs were obtained in a relatively straightforward manner and showed good proliferative capacity, even from a small amount of pulp tissue. RESULTS: Analysis by flow cytometry confirmed the characteristics of mesenchymal SCs with low expression of CD34 and CD45 antigens, which are markers for hematopoietic cells, and high levels of expression of CD105, CD166, CD90 and CD73 antigens, which are markers for mesenchymal SCs. Cell plasticity was confirmed by identifying calcium deposits in cultures that received osteogenic medium, and intracellular lipid accumulation in adipogenic cultures that received adipogenic medium. CONCLUSIONS: SCs collected from deciduous teeth show promising potential for application in tissue regeneration. Therefore, it is important that knowledge about the existence and characteristics of this source of stem cells be disseminated among dentists and that the technique, its limitations and possible indications are highlighted and discussed

Physalins B and F, seco-steroids isolated from Physalis angulata L., strongly inhibit proliferation, ultrastructure and infectivity of Trypanosoma cruzi.

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-07-07T19:07:08Z No. of bitstreams: 1 Meira CS Physalins B and F....pdf: 573771 bytes, checksum: 2e9ab6264149abbb0df24fafaa90c499 (MD5)Made available in DSpace on 2014-07-07T19:07:08Z (GMT). No. of bitstreams: 1 Meira CS Physalins B and F....pdf: 573771 bytes, checksum: 2e9ab6264149abbb0df24fafaa90c499 (MD5) Previous issue date: 2013Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade do Estado da Bahia. Departamento de Ciências da Vida. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos (Farmanguinhos). Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos (Farmanguinhos). Rio de Janeiro, RJ, BrasilHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilWe previously observed that physalins have immunomodulatory properties, as well as antileishmanial and antiplasmodial activities. Here, we investigated the anti-Trypanosoma cruzi activity of physalins B, D, F and G. We found that physalins B and F were the most potent compounds against trypomastigote and epimastigote forms of T. cruzi. Electron microscopy of trypomastigotes incubated with physalin B showed disruption of kinetoplast, alterations in Golgi apparatus and endoplasmic reticulum, followed by the formation of myelin-like figures, which were stained with MDC to confirm their autophagic vacuole identity. Physalin B-mediated alteration in Golgi apparatus was likely due to T. cruzi protease perturbation; however physalins did not inhibit activity of the trypanosomal protease cruzain. Flow cytometry examination showed that cell death is mainly caused by necrosis. Treatment with physalins reduced the invasion process, as well as intracellular parasite development in macrophage cell culture, with a potency similar to benznidazole. We observed that a combination of physalins and benznidazole has a greater anti-T. cruzi activity than when compounds were used alone. These results indicate that physalins, specifically B and F, are potent and selective trypanocidal agents. They cause structural alterations and induce autophagy, which ultimately lead to parasite cell death by a necrotic process

Activity of physalins purified from Physalis angulata in in vitro and in vivo models of cutaneous leishmaniasis

Submitted by Martha Silveira Berbert ([email protected]) on 2011-04-13T03:45:34Z No. of bitstreams: 1 Activity of physalins purified from Physalis angulata.pdf: 268630 bytes, checksum: 4a5b191f190a55e49bea0e060ae47bec (MD5)Made available in DSpace on 2011-04-13T03:45:34Z (GMT). No. of bitstreams: 1 Activity of physalins purified from Physalis angulata.pdf: 268630 bytes, checksum: 4a5b191f190a55e49bea0e060ae47bec (MD5) Previous issue date: 2009-05-19Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.We have previously demonstrated the immunomodulatory effects of physalins, secosteroids purified from Physalis angulata. Here we investigate the antileishmanial activity of physalins in vitro and in vivo in a model of cutaneous leishmaniasis.The antileishmanial activity of physalins B, D and F was tested in Leishmania-infected macrophage cultures. For the in vivo studies, BALB/c mice were infected with Leishmania amazonensis subcutaneously in the ear pinna and treated with physalin F by topical administration.Physalins B and F were able to reduce the percentage of Leishmania-infected macrophages and the intracellular parasite number in vitro at concentrations non-cytotoxic to macrophages. More importantly, topical treatment with physalin F significantly reduced the lesion size, the parasite load and histopathological alterations in BALB/c mice infected with L. amazonensis.Our results demonstrate the potent antileishmanial activity of physalins, especially physalin F, and suggest these molecules as the basis for the development of new therapeutic options for cutaneous leishmaniasis

A New Source of (R)-Limonene and Rotundifolone from Leaves of Lippia pedunculosa (Verbenaceae) and their Trypanocidal Properties

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2017-08-17T17:05:21Z No. of bitstreams: 1 Menezes L A new source....pdf: 240902 bytes, checksum: 6fac98c7e75cc4d38f9095f35e677c25 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2017-08-17T17:17:45Z (GMT) No. of bitstreams: 1 Menezes L A new source....pdf: 240902 bytes, checksum: 6fac98c7e75cc4d38f9095f35e677c25 (MD5)Made available in DSpace on 2017-08-17T17:17:45Z (GMT). No. of bitstreams: 1 Menezes L A new source....pdf: 240902 bytes, checksum: 6fac98c7e75cc4d38f9095f35e677c25 (MD5) Previous issue date: 2014FAPITEC/SE (Editais No 07/2009 and 10/2009), FAPESB, CNPq, FINEP, UFPR and CAPESUniversidade Federal de Sergipe. Departamento de Química. São Cristóvão, SE, BrasilUniversidade Federal de Sergipe. Departamento de Química. São Cristóvão, SE, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade do Estado da Bahia. Departamento de Ciências da Vida. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade do Estado da Bahia. Departamento de Ciências da Vida. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilUniversidade Federal do Paraná. Departamento de Química. Curitiba, PR, BrasilUniversidade Federal do Paraná. Departamento de Química. Curitiba, PR, BrasilUniversidade Federal de Sergipe. Departamento de Química. Itabaiana, SE, BrasilInvestigation by GC-FID and GC-MS of the essential oil (LPOE) from the leaves of Lippia pedunculosa revealed, as the major compounds, the monoterpenes rotundifolone (71.7%) and (R)-limonene (21.8%). These two compounds and the minor constituent piperitenone (1.2%) were also isolated from the leaves and identified by spectrometric analysis. LPOE and isolated compounds were evaluated for their trypanocidal activity against epimastigote and trypomastigote forms of Trypanosoma cruzi. Significant results with IC50 values lower than 34.0 g.mL-1 were observed against these forms of T. cruzi for LPOE and isolated compounds. Rotundifolone was the most active compound with an IC50 lower than 10.0 g.mL-1 for both forms of T. cruzi. The effects of LPOE and isolated compounds were also evaluated in cultures of macrophages infected with T. cruzi. Treatment with (R)-limonene and rotundifolone caused a moderate reduction in the percentage of macrophages infected by T. cruzi and in the number of intracellular parasites at concentrations non-toxic to macrophages

Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-02-18T13:02:23Z No. of bitstreams: 1 Guimarães. ET. et al. Mesenchymal and...Arch Oral Biol 2011. v. 56, p. 1247.pdf: 1457788 bytes, checksum: ba55b4a1d85752be8377b36354ea9945 (MD5)Made available in DSpace on 2014-02-18T13:02:23Z (GMT). No. of bitstreams: 1 Guimarães. ET. et al. Mesenchymal and...Arch Oral Biol 2011. v. 56, p. 1247.pdf: 1457788 bytes, checksum: ba55b4a1d85752be8377b36354ea9945 (MD5) Previous issue date: 2011Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Laboratório de Genética Humana e Mutagênese. Departamento de Biologia Geral. Salvador, BA, BrasilUniversidade de São Paulo. Faculdade de Medicina. Departamento de Urologia. Botucatu, SP, Brasil / Centro de Pesquisas Hospital A.C. Camargo. São Paulo, SP, BrasilUniversidade de São Paulo. Instituto de Biociências. Departamento de Biologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilObjective: Several studies have demonstrated that human dental pulp is a source of mesenchymal stem cells. To better understand the biological properties of these cells we isolated and characterized stem cells from the dental pulp of EGFP transgenic mice. Methods: The pulp tissue was gently separated from the roots of teeth extracted from C57BL/ 6 mice, and cultured under appropriate conditions. Flow cytometry, RT-PCR, light microscopy (staining for alkaline phosphatase) and immunofluorescence were used to investigate the expression of stem cell markers. The presence of chromosomal abnormalities was evaluated by G banding. Results: The mouse dental pulp stem cells (mDPSC) were highly proliferative, plasticadherent, and exhibited a polymorphic morphology predominantly with stellate or fusiform shapes. The presence of cell clusters was observed in cultures of mDPSC. Some cells were positive for alkaline phosphatase. The karyotype was normal until the 5th passage. The Pou5f1/Oct-4 and ZFP42/Rex-1, but not Nanog transcripts were detected in mDPSC. Flow cytometry and fluorescence analyses revealed the presence of a heterogeneous population positive for embryonic and mesenchymal cell markers. Adipogenic, chondrogenic and osteogenic differentiation was achieved after two weeks of cell culture under chemically defined in vitro conditions. In addition, some elongated cells spontaneously acquired a contraction capacity. Conclusions: Our results reinforce that the dental pulp is an important source of adult stem cells and encourage studies on therapeutic potential of mDPSC in experimental disease model