11 research outputs found

    data_Derroire_et_al_2016_Oikos

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    The provided data give the values of diversity and similarity indices for trees and shrubs, the successional age of the plot and values for environmental factors for 829 plots of 13 chronosequences in successional tropical dry forests used in the meta-analyses presented in the paper

    Additional file 11: Figure S3. of Habitat properties are key drivers of Borrelia burgdorferi (s.l.) prevalence in Ixodes ricinus populations of deciduous forest fragments

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    Response profiles of infection prevalence for each significant driver. Shown are the prediction line, confidence band (alpha = 0.05) and the partial residuals. The driver groups are macroclimate, landscape, macrohabitat, microhabitat and ontogeny. η2 values represent the relative contribution each variable has in explaining variation in the response. Adult infection prevalence. (FA) = variable is a correlation factor, ‘abund.’ = abundance, ‘disp.’ = dispersules, ‘SLA’ = specific leaf area, ‘tree2’ = lower tree layer. (PDF 254 kb

    DataSheet_1_Metabarcoding of soil environmental DNA to estimate plant diversity globally.pdf

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    IntroductionTraditional approaches to collecting large-scale biodiversity data pose huge logistical and technical challenges. We aimed to assess how a comparatively simple method based on sequencing environmental DNA (eDNA) characterises global variation in plant diversity and community composition compared with data derived from traditional plant inventory methods.MethodsWe sequenced a short fragment (P6 loop) of the chloroplast trnL intron from from 325 globally distributed soil samples and compared estimates of diversity and composition with those derived from traditional sources based on empirical (GBIF) or extrapolated plant distribution and diversity data.ResultsLarge-scale plant diversity and community composition patterns revealed by sequencing eDNA were broadly in accordance with those derived from traditional sources. The success of the eDNA taxonomy assignment, and the overlap of taxon lists between eDNA and GBIF, was greatest at moderate to high latitudes of the northern hemisphere. On average, around half (mean: 51.5% SD 17.6) of local GBIF records were represented in eDNA databases at the species level, depending on the geographic region.DiscussioneDNA trnL gene sequencing data accurately represent global patterns in plant diversity and composition and thus can provide a basis for large-scale vegetation studies. Important experimental considerations for plant eDNA studies include using a sampling volume and design to maximise the number of taxa detected and optimising the sequencing depth. However, increasing the coverage of reference sequence databases would yield the most significant improvements in the accuracy of taxonomic assignments made using the P6 loop of the trnL region.</p
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