<i>Vibrio superstes</i> sp. nov., isolated from the gut of Australian abalones <i>Haliotis laevigata</i> and <i>Haliotis rubra</i>

Five alginolytic, facultatively anaerobic, non-motile bacteria were isolated from the gut of abalones Haliotis laevigata and Haliotis rubra. Phylogenetic analyses based on 16S rDNA data indicated that these strains are related closely to Vibrio halioticoli (98 % 16S rDNA sequence similarity). DNA–DNA hybridization and fluorescent amplified fragment length polymorphism fingerprinting demonstrated that the five strains constituted a single species that was different from all currently known vibrios. The name Vibrio superstes sp. nov. (type strain, LMG 21323T=IAM 15009T=G3-29T; DNA G+C content, 48·0–48·9 mol%) is proposed to encompass this novel taxon. Several phenotypic features were disclosed that discriminate V. superstes from other Vibrio species: V. superstes sp. nov. and V. halioticoli can be differentiated on the basis of 17 traits (indole production, β-galactosidase test and assimilation of 15 carbon compounds)

Aquatic Animal Health Subprogram: Development of improved molecular diagnostic tests for Perkinsus olseni in Australian molluscs

Perkinsus is the widespread cause of disease and lost production in mollusc fisheries world - wide. Found mostly in temperate waters, two species are listed internationally as notifiable by the OIE and also appear on Australia's National List of Reportable Diseases of Aquatic Animals. Although Perkinsus marinus is exotic to Australia, Perkinsus olseni is enzootic and well - known as the cause of serious infections in various wild abalone populations in south - eastern Australia. The rapid identification and reliable differentiation of species is a major issue in the diagnosis and management of Perkinsosis in Australia. Traditional methods of Perkinsus diagnosis, such as histology and Ray’s thioglycollate culture, are straightforward and practical, however they lack sensitivity and fail to differentiate specific species. The molecular methods currently recommended by the OIE are based on conventional 1 - step PCR which is generally more labour intensive, slower and less sensitive than real - time PCR. The primary aim of this project was to develop and validate a species - specific real - time PCR (qPCR) assay for Perkinsus olsen

Vibrio superstes sp nov., isolated from the gut of Australian abalones Haliotis laevigata and Haliotis rubra

Five alginolytic, facultatively anaerobic, non-motile bacteria were isolated from the gut of abalones Haliotis laevigata and Haliotis rubra. Phylogenetic analyses based on 16S rDNA data indicated that these strains are related closely to Vibrio halioticoli (98 % 16S rDNA sequence similarity). DNA–DNA hybridization and fluorescent amplified fragment length polymorphism fingerprinting demonstrated that the five strains constituted a single species that was different from all currently known vibrios. The name Vibrio superstes sp. nov. (type strain, LMG 21323T=IAM 15009T=G3-29T; DNA G+C content, 48·0–48·9 mol%) is proposed to encompass this novel taxon. Several phenotypic features were disclosed that discriminate V. superstes from other Vibrio species: V. superstes sp. nov. and V. halioticoli can be differentiated on the basis of 17 traits (indole production, β-galactosidase test and assimilation of 15 carbon compounds)