44 research outputs found
Fetal Echocardiogram Normal and Abnormal
In this chapter, the normal anatomy of the heart as well as pathologic cases is consistent with cardiac malposition and isomerism, septal defects, pulmonary stenosis/atresia/absent pulmonary valve syndrome, aortic malformation, hypoplastic left heart, conotruncal anomalies/common arterial trunk, tricuspid dysplasia, Ebstein anomaly, univentricular heart, and systemic venous abnormalities among other congenital cardio vascular defects by ultrasound images. Anatomical details of most CHD in fetus were provided by two-dimensional (2D) ultrasound with higher quality imaging, which enhances the diagnostic accuracy in a variety of CHD
Clinical and immunological responses of zidovudine lamivudine-nevirapine versus tenofovir lamivudine-efavirenz antiretroviral treatment among HIV-1 infected adults: Gandhi Hospital, Telangana, India
Background: HAART (Highly active antiretroviral therapy) is the cornerstone of management of patients with HIV infection. Antiretroviral therapy was started in the year 1986 with the first drug Zidovudine (ZDV). Later on, other antiretroviral drugs (NRTIs, NNRTIs and Pls) were introduced. Dual and mono therapies were used initially but the problem of resistance emerged. Currently, 3 or more ARV drugs are recommended globally for the treatment of people with HIV infection.Methods: A cross-sectional descriptive study conducted at a tertiary care Hospital over 200 patients, two commonly used medications are ZLN (Zidovudine+Lamivudine+Nevirapine) and TLE (Tenofovir+Lamivudine+Efavirenz ). The factors considered to affect the clinical and immunologic outcomes in both groups were assessed using baseline CD4 count, WHO clinical staging, presence of chronic diarrhea, anemia, and baseline weight, occurrence of TB, and switching of ART regimen.Results: A total of 200 patients were included in the study. ART documents of 100 patients are on Zidovudine+Lamivudine+Nevirapine) and 100 patients are on TLE (Tenofovir+Lamivudine+Efavirenz) regimen. Out of 200 patients, 97 were males and 103 were females. Maximum number of subjects were in the age of 15-45 years (82.5%) followed by 45 and above (17.5%). Mean age was 34.5±2.5 (years) with range 15 to 65 years. The baseline CD4 count of the patients, 94 were <350 and 6 were ≥350 on ZLN, in case of TLE 82 were <350 and 18 were ≥350. CD4 count after 6 months in 200 patients as follows, 60 were <350 and 40 were ≥350 in case of TLE 53 were <350 and 47 were ≥350.Conclusions: This research finding concluded that there is no critical difference between the two medications in regards to serious adverse events but did find that TDF is superior to AZT in terms of immunologic response and adherence and more frequent emergence of resistance
BIOGENIC SYNTHESIS OF SILVER NANOPARTICLES AND THEIR SYNERGISTIC EFFECT WITH ANTIBIOTICS: A STUDY AGAINST SALMONELLA SP
Objective: In recent era, medical science has turned to a variety of novel products and technologies to halt the spread of infections. The most promising and effective antimicrobial agents being embraced by modern medical science today is silver. With increasing drug-resistance and growing concern regarding the over-prescribing of antibiotics, there has been a resurgent interest in the use of antimicrobial silver. Unlike antibiotics, silver appears to be immune to resistance. Thus, the conjugation of antibiotic with silver nanoparticles would prevent development of resistance of microbes and increase the antimicrobial property of the antibiotic.Methods: In this manuscript, extracellular biological synthesis of silver nanoparticles was made from Trametes sp. The characterization of the nanoparticles was carried out as well as its antibiotic efficacy was evaluated in addition to the antibiotic combination.Results: Initially the fungal cell filtrate was challenged with 1Mm AgNO3 solution, in a 250 ml Erlenmeyer flask and agitated at 30 °C in dark for 72 h under static conditions at 200 rpm and the silver nanoparticle production was monitored by the change in color and later it was subjected to optical measurements by UV-Vis spectrophotometer. The characters of these silver nanoparticles were further studied by Fourier Transform Infrared (FTIR) Spectroscopy, SEM and TEM studies. The nanoparticles produced during the study period were found to have wider antibacterial property and also it showed the enhanced efficacy in combination with ceftriaxone and ofloxacin against Salmonella sp.Conclusion: The synergistic mode of antibiosis in between nanoparticles synthesized from Trametes sp. and ceftriaxone, ofloxacin was found to be more effective against Salmonella sp. in the current study.Â
Biological Agents for the Synthesis of Silver Nanoparticles and their Applications
The field of nanotechnology is experiencing rapid growth owing to its distinctive functionality and diverse range of applications. Nanomedicine is a field of study that investigates the potential applications of nanotechnology in the areas of disease prevention, treatment, diagnosis, and control. The significance of silver nanoparticles lies in their distinct characteristics, capacity to generate varied nanostructures, extensive range of bactericidal and anticancer properties, wound healing and other therapeutic capabilities, and cost-effectiveness in manufacturing. These nanoparticles are particularly noteworthy due to their size, which can range from 1 to 100 nm. This paper provides an overview of diverse physical, chemical, and biological techniques employed for the synthesis of silver nanoparticles. The text delineates various methodologies utilizing silver nanoparticles as agents for combating microbial and biofilm infections, as well as for their potential as antitumorigenic agents. Additionally, the text explores the applications of silver nanoparticles in the field of dentistry and dental implants, their role in promoting bone regeneration, their use in cardiovascular implants, and their potential as promoters of wound healing. The present study investigates the mechanism of action, synthesis techniques, and morphological characterization of silver nanoparticles in order to evaluate their potential applications in medical therapies and disease control
Evaluation of Antibacterial Activity of Bryopsis pennata and Chaetomorpha antennina against Multidrug Resistant Morganella morganii and Salmonella species Isolated from Healthy Individuals
The problem of antibiotic resistance is fast becoming a pandemic which has necessitated the need
for new drugs discovery. This study was carried out to screen two green algal species- Bryopsis
pennata and Chaetomorpha anteninna for antibacterial activity against multidrug resistant
pathogenic enteric organisms (Morganella morganii and Salmonella species) obtained from healthy
individuals. Algal samples were obtained and processed. Crude extraction was carried out with dichloromethane/methanol (2:1) while the antibacterial screening was done by agar-well diffusion
method. Results revealed that M. morganii was 3.37% of the total isolates recovered while
Salmonella species was 6.74%. Result also showed that C. antennina was active against all the
strains of Salmonella species with inhibitory zones ranging from 10 mm to 17 mm and the
M. morganii with inhibitory zone of 11 mm while B. pennata showed inhibitory activities against only
S. pullorum and S. enterica subspecies diarizonae with inhibitory zones of 12 mm and 7 mm
respectively as well as the M. morganii strain with 14 mm. The antibacterial activities observed from
these green algae showed that Bryopsis pennata and Chaetomorpha antennina from the West
African coast are promising in the quest for new drugs with potentials against multidrug resistant
strains of bacteria and therefore should be intensely researched int
Biogenic Synthesis and Cytotoxic Effects of Silver Nanoparticles Mediated by White Rot Fungi
Silver nanoparticles (AgNPs) were successfully synthesized using silver nitrate via the biological route using the culture filtrate of Ganoderma enigmaticum as well as Trametes ljubarskyi white rot fungi materials at room temperature. The proposed synthetic technique was applied for the first time for AgNPs preparation via the biological route through a low-cost pathway, which considered as an adequate direction of preparation compared to the commercial methods. This study reports the in vitro cytotoxic effect of biologically synthesized AgNPs in disposing of the human lung cancer cell line (A549) and human breast cancer cell (MCF-7) by using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. In addition, the viability of the tested cell lines was tested after treatment for 24 h in the presence of the prepared nanoparticles. The obtained results indicated the reduced viability of cancer cell lines with improving concentrations of AgNPs (40-120 mug/mL) at 24 h. Furthermore, at 120 mug/mL concentration, the fungal nanoparticles showed substantial cytotoxic effects toward the treated cells. Consequently, the results designated that the biologically synthesized silver nanoparticles have effective behavior for treating A549 and MCF-7 cancer cells from the laboratory experiment approach; however, additional studies are required to validate these results in vivo models as anticancer agents depending on their cytotoxic activit
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Actionable Knowledge Discovery using Multi-Step Mining
Data mining at enterprise level operates on huge amount of
data such as government transactions, banks, insurance
companies and so on. Inevitably, these businesses produce
complex data that might be distributed in nature. When
mining is made on such data with a single-step, it produces
business intelligence as a particular aspect. However, this
is not sufficient in enterprise where different aspects and
standpoints are to be considered before taking business
decisions. It is required that the enterprises perform mining
based on multiple features, data sources and methods. This
is known as combined mining. The combined mining can
produce patterns that reflect all aspects of the enterprise.
Thus the derived intelligence can be used to take business
decisions that lead to profits. This kind of knowledge is
known as actionable knowledge.Data mining is a process of obtaining trends or patterns in historical data. Such trends form business intelligence that in turn leads to taking well informed decisions. However, data mining with a single technique does not yield actionable knowledge. This is because enterprises have huge databases and heterogeneous in nature. They also have complex data and mining such data needs multi-step mining instead of single step mining. When multiple approaches are involved, they provide business intelligence in all aspects. That kind of information can lead to actionable knowledge. Recently data mining has got tremendous usage in the real world. The drawback of existing approaches is that insufficient business intelligence in case of huge enterprises. This paper presents the combination of existing works and algorithms. We work on multiple data sources, multiple methods and multiple features. The combined patterns thus obtained from complex business data provide actionable knowledge. A prototype application has been built to test the efficiency of the proposed framework which combines multiple data sources, multiple methods and multiple features in mining process. The empirical results revealed that the proposed approach is effective and can be used in the real world
Seroprevalence of Chikungunya virus infection in a Tertiary Care Hospital, Warangal, Telangana, South India: Seroprevalence of Chikungunya virus infection
Introduction:
The Chikungunya virus, an Alpha virus belongs to the Togaviridae family which infects via the vector Aedes spp. mosquitoes, is the cause of the Chikungunya sickness. The most prevalent symptoms are an abruptly rising temperature, rash, and acute arthralgia. Similar to other arboviral diseases such as Dengue and Japanese B encephalitis infections, it is a serious public health issue.
Methods: A total of 3027 blood samples were received from suspected cases of Chikungunya in the Tertiary Care Hospital, Warangal, Telangana. Over a period of 17 months, this study was conducted. Nearly 5-10ml of blood was collected from the patients after 5 days of fever, and the serum was separated. Using an IgM antibody capture ELISA kit manufactured by NIV (NIV, Pune, India).
Results: In our study population of 3027 during 12 months, 313 Chikungunya cases (10.34%) were found to be seropositive for Chikungunya by IgM ELISA. Most cases were from 0-10 Years (24.60%), followed by 11-20 years (17.25%). Regarding the sex ratio of the total affected cases, 53.35% were males, and 46.64% were females. A maximum number of positives were seen in September, November, and October, which were found to be 35.46%, 18.84%, and 17.57%.
Conclusion: The study found a seroprevalence rate of 23.06% for Chikungunya, with a notable increase in frequency during the monsoon season. Additionally, the virus was found to disproportionately affect individuals in the productive age range of the population
Isolation, Screening and Identification of Laccase Producing Fungi from Eturnagaram Forest, Warangal District, Telangana, India
Lacasse is one of the extracellular enzymes excreted from white and brown rot fungi, which is involved in ligninolysis. Laccases are N-glycosilated multi copper oxidases belonging to the group of the blue copper proteins. In fungi, laccase is present in Ascomycetes, Deuteromycetes, Basidiomycetes and is particularly abundant in many white-rot fungi that degrade lignin. Laccases have been subject of intensive research in the last decades due to their broad substrate specificity. In the recent years, their uses span from the textile to the pulp and paper industries, and food applications to bioremediation processes. Laccases also have uses in organic synthesis, where typical substrates are phenols and amines, and the reaction products are dimers and oligomers derived from the coupling of reactive radical intermediates. In this present study thirty white rot fungi were collected and investigated for highest laccase producing organisms in submerged fermentation. Among 30 cultures eighteen showed brown colour zone. Out of these, five isolates (Pv5, Pv3, Pv8, Pv11, and Pv12) had shown the brown colour zone from day one and Pv5 showed highest brown zone. This study describes the isolation of white rot fungi, their molecular identification and screening for their ability to produce laccase.. Based on sequence comparison and phylogenetic analysis with reference taxa the strain Pv5 was identified as Trametes sp