Indagine fitosanitaria nel comprensorio di Sarroch, Villa S. Pietro e Pula (prov. di Cagliari) in relazione all'attività di uno stabilimento petrolifero

A five-year investigation (1988-92) of the phytosanitary situation in the Sarroch, Villa S. Pietro and Pula areas, belonging to the Cagliari district, where the SARAS-oil company has been operating for a long time, was carried out. Various degrees of damage caused by fungi, bacteria, viruses, insects, acari and by abiotic agents (edaphic and climatic) were detected on trees and herbaceous plants. Investigations on possible S02 damages by means of phytosanitary observations and chemical analysis, clearly showed no noxious effects of this gas on both cultivated and spontaneous plants

Prognostic Role of CSF β-amyloid 1-42/1-40 Ratio in Patients Affected by Amyotrophic Lateral Sclerosis

The involvement of β-amyloid (Aβ) in the pathogenesis of amyotrophic lateral sclerosis (ALS) has been widely discussed and its role in the disease is still a matter of debate. Aβ accumulates in the cortex and the anterior horn neurons of ALS patients and seems to affect their survival. To clarify the role of cerebrospinal fluid (CSF) Aβ 1-42 and Aβ 42/40 ratios as a potential prognostic biomarker for ALS, we performed a retrospective observational study on a cohort of ALS patients who underwent a lumbar puncture at the time of the diagnosis. CSF Aβ 1-40 and Aβ 1-42 ratios were detected by chemiluminescence immunoassay and their values were correlated with clinical features. We found a significant correlation of the Aβ 42/40 ratio with age at onset and Mini Mental State Examination (MMSE) scores. No significant correlation of Aβ 1-42 or Aβ 42/40 ratios to the rate of progression of the disease were found. Furthermore, when we stratified patients according to Aβ 1-42 concentration and the Aβ 42/40 ratio, we found that patients with a lower Aβ 42/40 ratio showed a shorter survival. Our results support the hypothesis that Aβ 1-42 could be involved in some pathogenic mechanism of ALS and we suggest the Aβ 42/40 ratio as a potential prognostic biomarker

Design, synthesis and docking study of novel coumarin ligands as potential selective acetylcholinesterase inhibitors

<p>New coumaryl-thiazole derivatives with the acetamide moiety as a linker between the alkyl chains and/or the heterocycle nucleus were synthesized and <i>in vitro</i> tested as acetylcholinesterase (AChE) inhibitors. 2-(diethylamino)-N-(4-(2-oxo-2H-chromen-3-yl)thiazol-2-yl)acetamide (<b>6c</b>, IC₅₀ value of 43 nM) was the best AChE inhibitor with a selectivity index of 4151.16 over BuChE. Kinetic study of AChE inhibition revealed that <b>6c</b> was a mixed-type inhibitor. Moreover, the result of H4IIE hepatoma cell toxicity assay for <b>6c</b> showed negligible cell death. Molecular docking studies were also carried out to clarify the inhibition mode of the more active compounds. Best pose of compound <b>6c</b> is positioned into the active site with the coumarin ring wedged between the residues of the CAS and catalytic triad of AChE. In addition, the coumarin ring is anchored into the gorge of the enzyme by H-bond with Tyr130.</p