Biochemistry

www.elsevier.com/locate/yabio SigniWcant enhancement of Xuorescence on hybridization of a molecular beacon to a target DNA in the presence of a site-speciWc DNA nickas

Practical Implementation of the Algorithm for Processing Signals Obtained by Magnetic Flaw Detection of Rolled Steel

Various mathematical methods are used to process the signals obtained by using magnetic flaw detection of rolled steel. In the article [1], a method for increasing the resolution of magnetic flaw detection is proposed. © 2022 American Institute of Physics Inc.. All rights reserved.Minobrnauki of Russia, (AAAA-A18-118020690196-3)The research was carried out within the state assignment of Minobrnauki of Russia (theme “Diagnostics” No. AAAA-A18-118020690196-3)

Biological diversity of the coastal zone of the Crimean peninsula: problems, preservation and restoration pathways

The results of complex hydrochemical, hydrobiological and ichthyological investigations by IBSS, NAS of Ukraine, realized in 6 regions of the coastal zone of the Crimean peninsula in the Black Sea and the Sea of Azov are given. The main negative factors causing changes in structural and functional characteristics of hydrobiocenoses in the regions studied are analyzed and “hot ecological spots” are isolated. Variants of different methods of management of the coastal ecosystems, including construction of artificial reefs and usage of biological filters for water cleaning, protection and recreation of biological diversity are taken into consideration

The Insignificance of Global Reheating in the Abell 1068 Cluster: Multiwavelength Analysis

We present a detailed, multiwavelength study of the Abell 1068 galaxy cluster, and we use this data to test cooling and energy feedback models of galaxy clusters. Near ultraviolet and infrared images of the cluster show that the cD galaxy is experiencing star formation at a rate of ~20-70 M_o/yr over the past ~100 Myr. The dusty starburst is concentrated toward the nucleus of the cD galaxy and in filamentary structures projecting 60 kpc into its halo. The Chandra X-ray image presented in WMM reveals a steep temperature gradient that drops from roughly 4.8 keV beyond 120 kpc to roughly 2.3 keV in the inner 10 kpc of the galaxy where the starburst peaks. Over 95% of the ultraviolet and Halpha photons associated with the starburst are emerging from regions cloaked in keV gas with very short cooling times ~100 Myr, as would be expected from star formation fueled by cooling condensations in the intracluster medium. The local cooling rate in the vicinity of the central starburst is < 40 M_o/yr, which is consistent with the star formation rate determined with U-band and infrared data. We find that energy feedback from both the radio source and thermal conduction are inconsequential in Abell 1068. Although supernova explosions associated with the starburst may be able to retard by ~18% or so, they are incapable of maintaining the cooling gas at keV temperatures.Comment: 27 pages, 5 postscript figures, accepted to Ap

Theoretical Study on Superconductivity in Boron-Doped Diamond

We consider superconductivity in boron (B) doped diamond using a simplified model for the valence band of diamond. We treat the effects of substitutional disorder of B ions by the coherent potential approximation (CPA) and those of the attractive force between holes by the ladder approximation under the assumption of instantaneous interaction with the Debye cutoff. We thereby calculate the quasiparticle life time, the evolution of the single-particle spectra due to doping, and the effect of disorder on the superconducting critical temperature $T_c$. We in particular compare our results with those for supercell calculations to see the role of disorder, which turns out to be of crucial importance to $T_c$.Comment: 9 pages, 13 figures, submitted to J. Phys. Soc. Jpn., Errors in embedded eps figure files have been correcte

Physics and chemistry of hydrogen in the vacancies of semiconductors

Hydrogen is well known to cause electrical passivation of lattice vacancies in semiconductors. This effect follows from the chemical passivation of the dangling bonds. Recently it was found that H in the carbon vacancy of SiC forms a three-center bond with two silicon neighbors in the vacancy, and gives rise to a new electrically active state. In this paper we examine hydrogen in the anion vacancies of BN, AlN, and GaN. We find that three-center bonding of H is quite common and follows clear trends in terms of the second-neighbor distance in the lattice, the typical (two-center) hydrogen-host-atom bond length, the electronegativity difference between host atoms and hydrogen, as well as the charge state of the vacancy. Three-center bonding limits the number of H atoms a nitrogen vacancy can capture to two, and prevents electric passivation in GaAs as well

Research the Possibilities of Magnetic Flaw Detection in Terms of Detecting Defects in the Surface of a Pipe Billet

Non-destructive instrumental inspection of pipe billet surface defects is currently absent at most metallurgical plants. Only visual inspection is carried out. At the same time, this problem is very urgent and has not yet received a practical solution. The article describes the solution method

Recommendations for Increasing the Detection of Longitudinal Defects of the Inner Surface of Pipes with the Magnetic Control Method

The task of increasing the detection of longitudinal internal defects during magnetic flaw detection of oil grade pipes is very relevant and has not yet received a practical solution. This is especially true of the problem of detecting defects whose depth is no more than 5% of the nominal wall

Characteristics of neutrophilic granulocytes of peripheral blood in patients with mechanical jaundice caused by cholangiocarcinoma

Obstructive jaundice caused by cholangiocarcinoma takes a special place among malignant disorders and surgical pathology in Russia. Involvement of nonspecific immunity and the role of neutrophils in carcinogenesis are ambiguously evaluated. The aim of this study was to study functional activity of peripheral blood neutrophilic granulocytes and their phenotype in obstructive jaundice caused by cholangiocarcinoma. The study included 56 patients with obstructive jaundice associated with cholangiocarcinoma at the T2- 3N0- 1M0 stage (clinical stages II-III), and 90 apparently healthy volunteers of similar age group. Neutrophilic granulocytes were isolated from peripheral blood by means of double-density Ficoll-Urografin gradient.Venous blood was collected in patients into vacutainers with heparin upon admission to the hospital, before the surgery was performed. Spontaneous cytokine production (IL-8, IFNá) was determined by enzyme-linked immunosorbent assay using Vector-Best diagnostic kits. To assess phagocytic activity of neutrophilic granulocytes, the phagocytic index (according to Hamburger), number of phagocytes (according to Wright) and the index of completed phagocytosis (according to Rudik, 2006) were calculated. Immunophenotyping of neutrophilic granulocytes was carried out using an FC500 flow cytometer (Beckman Coulter, USA) with monoclonal antibodies to CD11b+, CD16+, CD95+. The results were statistically analyzed using the Statistica v.12.0 software (StatSoft Inc., USA). To assess intergroup differences, the nonparametric Kruskal-Wallis tests (for three or more comparison groups) and Mann-Whitney tests (for pairwise comparison) were used. Comparison of groups for a qualitative binary trait was carried out using the two-sided Fisher’s exact test. Data are presented as Median (25 quartile-75 quartile).The study of the functional activity of peripheral blood neutrophiles in obstructive jaundice patients caused by cholangiocarcinoma revealed an increase in their relative and absolute number, increased phagocytic index and decreased phagocytic number of neutrophilic granulocytes, increased expression of CD11b+, CD16+, CD95+ immunological markers. The changes in neutrophil secretory activity were characterized by a decrease in cytokine production (IL-2, IFNá). An increase in functional activity of neutrophilic granulocytes, along with a decrease in their cytokine production suggests that, in obstructive jaundice observed in cholangiocarcinoma at clinical stage T2-3N0-1M0, an equilibrium stage is revealed between the cells of immune system and malignant tumor