Computing axes of rotation for 4-axis CNC milling machine by calculating global visibility map from slice geometry

This thesis presents a new method to compute a global visibility map (GVM) in order to determine feasible axes of rotation for 4-axis CNC machining. The choice of the 4th-axis is very important because it directly determines the critical manufacturing components; visibility, accessibility and machinability of the part. As opposed to the considerable work in GVM computation, this thesis proposes an innovative approximation approach to compute GVM by utilizing slice geometry. One advantage of the method is that it is feature-free, thus avoiding feature extraction and identification. In addition, the method is computationally efficient, and can be easily parallelized in order to vastly increase speed. In this thesis, we further present a full implementation of the approach as a critical function in an automated process planning system for rapid prototyping

Statistical Investigation of the Widths of Supra-arcade Downflows Observed During a Solar Flare

Supra-arcade downflows (SADs) are dark voids descending towards the post-reconnection flare loops and exhibit obvious variation in properties like width. However, due to the lack of further statistical studies, the mechanism behind such variations hitherto remains elusive. Here we statistically investigated widths of 81 SADs observed in one flare by the Solar Dynamics Observatory (SDO). For each of SADs, six moments were selected with equal time intervals to measure their widths at different stages of their evolution. It is found that most SADs show a roughly monotonous width decrease during their descents, while some SADs with small initial widths can have complex evolutions. 3D reconstruction results based on SDO and Solar Terrestrial Relations Observatory Ahead (STEREO-A) images and thermal properties analysis reveal that differences in magnetic and plasma environments may result in that SADs in the north are overall wider than those in the south. Additionally, correlation analysis between the width and other parameters of SADs was further conducted and revealed that: (1) SADs with different initial widths show no significant differences in their temperature and density evolution characteristics; (2) SADs with small initial widths usually appear in lower heights, where more frequent collisions between SADs could lead to their intermittent acceleration, width increment, and curved trajectories. These results indicate that SADs with different initial widths are produced the same way while different environments (magnetic field or plasma) could affect their subsequent width evolutions.Comment: 15 pages, 12 figures, 1 table. Accepted for publication in MNRA

Computing tool accessibility of polyhedral models for toolpath planning in multi-axis machining

This dissertation focuses on three new methods for calculating visibility and accessibility, which contribute directly to the precise planning of setup and toolpaths in a Computer Numerical Control (CNC) machining process. They include 1) an approximate visibility determination method; 2) an approximate accessibility determination method and 3) a hybrid visibility determination method with an innovative computation time reduction strategy. All three methods are intended for polyhedral models. First, visibility defines the directions of rays from which a surface of a 3D model is visible. Such can be used to guide machine tools that reach part surfaces in material removal processes. In this work, we present a new method that calculates visibility based on 2D slices of a polyhedron. Then we show how visibility results determine a set of feasible axes of rotation for a part. This method effectively reduces a 3D problem to a 2D one and is embarrassingly parallelizable in nature. It is an approximate method with controllable accuracy and resolution. The method’s time complexity is linear to both the number of polyhedron’s facets and number of slices. Lastly, due to representing visibility as geodesics, this method enables a quick visible region identification technique which can be used to locate the rough boundary of true visibility. Second, tool accessibility defines the directions of rays from which a surface of a 3D model is accessible by a machine tool (a tool’s body is included for collision avoidance). In this work, we present a method that computes a ball-end tool’s accessibility as visibility on the offset surface. The results contain all feasible orientations for a surface instead of a Boolean answer. Such visibility-to-accessibility conversion is also compatible with various kinds of facet-based visibility methods. Third, we introduce a hybrid method for near-exact visibility. It incorporates an exact visibility method and an approximate visibility method aiming to balance computation time and accuracy. The approximate method is used to divide the visibility space into three subspaces; the visibility of two of them are fully determined. The exact method is then used to determine the exact visibility boundary in the subspace whose visibility is undetermined. Since the exact method can be used alone to determine visibility, this method can be viewed as an efficiency improvement for it. Essentially, this method reduces the processing time for exact computation at the cost of introducing approximate computation overhead. It also provides control over the ratio of exact-approximate computation

The Prognosis of Anti-Angiogenesis Treatments Combined with Standard Therapy for Newly Diagnosed Glioblastoma: A Meta-Analysis of Randomized Controlled Trials

Background and Purpose: Although bevacizumab (BV) has been approved as second-line therapy for recurrent glioblastoma (GB), the efficacy and safety of BV for patients with newly diagnosed GB remain unclear. Methodology/Principal Findings: We systematically searched electronic databases (PubMed, EMBASE, OVID, etc.) to identify related studies published from January 1966 and August 2016. Eight randomized controlled trials including a total of 2,185 patients with GB were included. We found that the median progression-free survival (PFS) was higher in the BV group than in the standard therapy (ST) group (pooled hazard ratio, 0.73;95% CI, 0.62-0.86;P = 0.0001). Compared with ST, BV improved the PFS rate at 6 months (OR 3.33, 95% CI 2.73-4.06, p<0.00001) and 12 months (OR 2.10, 95% CI 1.74-2.54, p< 0.00001). There were no significant differences in median overall survival between the BV and ST groups (OR, 1.01;95% CI, 0.83-1.23;P = 0.95). The BV group had higher survival rates at 6 months (OR, 1.41;95% CI, 1.09-1.84;P = 0.01) and 12 months (OR, 1.23;95% CI, 1.02-1.48;P = 0.03), but a low survival rate at the 36-month follow-up (OR, 0.57;95% CI, 0.32-0.98;P = 0.04). For the incidence of adverse events, three adverse outcomes were found to be significantly different between BV and ST groups, including hypertension (8.37% vs. 1.62%, p<0.000001), proteinuria (7.65% vs. 0%, p<0.001), and fatigue (14.54% vs. 9.01%, p = 0.05). Conclusions/Significance: Our study indicates that combination of BV with ST for newly diagnosed GB did not improve the median overall survival but result in longer median PFS, maintaining the quality of life and functional status. However, the long-term use of BV is associated with a higher incidence of adverse events and mortality

Development of a recombinase-aided amplification combined with a lateral flow dipstick assay for rapid detection of H7 subtype avian influenza virus

Avian influenza viruses (AIV) pose a significant persistent threat to the public health and safety. It is estimated that there have been over 100 outbreaks caused by various H7 subtypes of avian influenza viruses (AIV-H7) worldwide, resulting in over 33 million deaths of poultry. In this study, we developed a recombinase-aided amplification combined with a lateral flow dipstick assay for the detection of hemagglutinin (HA) genes to provide technical support for rapid clinical detection of AIV-H7. The results showed that the assay can complete the reaction within 30 min at a temperature of 39°C. Specificity tests demonstrated that there was no cross-reactivity with other common poultry pathogens, including Newcastle disease virus (NDV) and infections bronchitis virus (IBV). The detection limit of this assay was 1 × 101 copies/μL, while RT-qPCR method was 1 × 101 copies/μL, and RT-PCR was 1 × 102 copies/μL. The κ value of the RT-RAA-LFD and RT-PCR assay in 132 avian clinical samples was 0.9169 (p &lt; 0.001). These results indicated that the developed RT-RAA-LFD assay had good specificity, sensitivity, stability and repeatability and may be used for rapid detection of AIV-H7 in clinical diagnosis

Arabidopsis Hormone Database: a comprehensive genetic and phenotypic information database for plant hormone research in Arabidopsis

Plant hormones are small organic molecules that influence almost every aspect of plant growth and development. Genetic and molecular studies have revealed a large number of genes that are involved in responses to numerous plant hormones, including auxin, gibberellin, cytokinin, abscisic acid, ethylene, jasmonic acid, salicylic acid, and brassinosteroid. Here, we develop an Arabidopsis hormone database, which aims to provide a systematic and comprehensive view of genes participating in plant hormonal regulation, as well as morphological phenotypes controlled by plant hormones. Based on data from mutant studies, transgenic analysis and gene ontology (GO) annotation, we have identified a total of 1026 genes in the Arabidopsis genome that participate in plant hormone functions. Meanwhile, a phenotype ontology is developed to precisely describe myriad hormone-regulated morphological processes with standardized vocabularies. A web interface (http://ahd.cbi.pku.edu.cn) would allow users to quickly get access to information about these hormone-related genes, including sequences, functional category, mutant information, phenotypic description, microarray data and linked publications. Several applications of this database in studying plant hormonal regulation and hormone cross-talk will be presented and discussed

Knockout of CAFFEOYL-COA 3-O-METHYLTRANSFERASE 6/6L enhances the S/G ratio of lignin monomers and disease resistance in Nicotiana tabacum

BackgroundNicotiana tabacum is an important economic crop, which is widely planted in the world. Lignin is very important for maintaining the physiological and stress-resistant functions of tobacco. However, higher lignin content will produce lignin gas, which is not conducive to the formation of tobacco quality. To date, how to precisely fine-tune lignin content or composition remains unclear.ResultsHere, we annotated and screened 14 CCoAOMTs in Nicotiana tabacum and obtained homozygous double mutants of CCoAOMT6 and CCoAOMT6L through CRSIPR/Cas9 technology. The phenotype showed that the double mutants have better growth than the wild type whereas the S/G ratio increased and the total sugar decreased. Resistance against the pathogen test and the extract inhibition test showed that the transgenic tobacco has stronger resistance to tobacco bacterial wilt and brown spot disease, which are infected by Ralstonia solanacearum and Alternaria alternata, respectively. The combined analysis of metabolome and transcriptome in the leaves and roots suggested that the changes of phenylpropane and terpene metabolism are mainly responsible for these phenotypes. Furthermore, the molecular docking indicated that the upregulated metabolites, such as soyasaponin Bb, improve the disease resistance due to highly stable binding with tyrosyl-tRNA synthetase targets in Ralstonia solanacearum and Alternaria alternata.ConclusionsCAFFEOYL-COA 3-O-METHYLTRANSFERASE 6/6L can regulate the S/G ratio of lignin monomers and may affect tobacco bacterial wilt and brown spot disease resistance by disturbing phenylpropane and terpene metabolism in leaves and roots of Nicotiana tabacum, such as soyasaponin Bb

A Survey of Avian Influenza in Tree Sparrows in China in 2011

Tree sparrows (Passer montanus) are widely distributed in all seasons in many countries. In this study, a survey and relevant experiments on avian influenza (AI) in tree sparrows were conducted. The results suggested that the receptor for avian influenza viruses (AIVs), SAα2,3Gal, is abundant in the respiratory tract of tree sparrows, and most of the tree sparrows infected experimentally with two H5 subtype highly pathogenic avian influenza (HPAI) viruses died within five days after inoculation. Furthermore, no AIVs were isolated from the rectum eluate of 1300 tree sparrows, but 94 serological positives of AI were found in 800 tree sparrows. The serological positives were more prevalent for H5 subtype HPAI (94/800) than for H7 subtype AI (0/800), more prevalent for clade 2.3.2.1 H5 subtype HPAI (89/800) than for clade 2.3.4 (1/800) and clade 7.2 (4/800) H5 subtype HPAI, more prevalent for clade 2.3.2.1 H5 subtype HPAI in a city in southern China (82/800) than in a city in northern China (8/800). The serological data are all consistent with the distribution of the subtypes or clades of AI in poultry in China. Previously, sparrows or other passerine birds were often found to be pathogenically negative for AIVs, except when an AIV was circulating in the local poultry, or the tested passerine birds were from a region near waterfowl-rich bodies of water. Taken together, the data suggest that tree sparrows are susceptible to infection of AIVs, and surveys targeting sparrows can provide good serological data about the circulation of AIVs in relevant regions

Panorama Phylogenetic Diversity and Distribution of Type A Influenza Virus

Type A influenza virus is one of important pathogens of various animals, including humans, pigs, horses, marine mammals and birds. Currently, the viral type has been classified into 16 hemagglutinin and 9 neuraminidase subtypes, but the phylogenetic diversity and distribution within the viral type largely remain unclear from the whole view.The panorama phylogenetic trees of influenza A viruses were calculated with representative sequences selected from approximately 23000 candidates available in GenBank using web servers in NCBI and the software MEGA 4.0. Lineages and sublineages were classified according to genetic distances, topology of the phylogenetic trees and distributions of the viruses in hosts, regions and time.Here, two panorama phylogenetic trees of type A influenza virus covering all the 16 hemagglutinin subtypes and 9 neuraminidase subtypes, respectively, were generated. The trees provided us whole views and some novel information to recognize influenza A viruses including that some subtypes of avian influenza viruses are more complicated than Eurasian and North American lineages as we thought in the past. They also provide us a framework to generalize the history and explore the future of the viral circulation and evolution in different kinds of hosts. In addition, a simple and comprehensive nomenclature system for the dozens of lineages and sublineages identified within the viral type was proposed, which if universally accepted, will facilitate communications on the viral evolution, ecology and epidemiology