The GATA factor HANABA TARANU promotes runner formation by regulating axillary bud initiation and outgrowth in cultivated strawberry

A runner, as an elongated branch, develops from the axillary bud (AXB) in the leaf axil and is crucial for the clonal propagation of cultivated strawberry (Fragaria x ananassa Duch.). Runner formation occurs in at least two steps: AXB initiation and AXB outgrowth. HANABA TARANU (HAN ) encodes a GATA transcription factor that affects AXB initiation in Arabidopsis and promotes branching in grass species, but the underlying mechanism is largely unknown. Here, the function of a strawberry HAN homolog FaHAN in runner formation was characterized. FaHAN transcripts can be detected in the leaf axils. Overexpression (OE) of FaHAN increased the number of runners, mainly by enhancing AXB outgrowth, in strawberry. The expression of the strawberry homolog of BRANCHED1 , a key inhibitor of AXB outgrowth in many plant species, was significantly downregulated in the AXBs of FaHAN -OE lines, whereas the expression of the strawberry homolog of SHOOT MERISTEMLESS, a marker gene for AXB initiation in Arabidopsis, was upregulated. Moreover, several genes of gibberellin biosynthesis and cytokinin signaling pathways were activated, whereas the auxin response pathway genes were repressed. Further assays indicated that FaHAN could be directly activated by FaNAC2, the overexpression of which in strawberry also increased the number of runners. The silencing of FaNAC2 or FaHAN inhibited AXB initiation and led to a higher proportion of dormant AXBs, confirming their roles in the control of runner formation. Taken together, our results revealed a FaNAC2-FaHAN pathway in the control of runner formation and have provided a means to enhance the vegetative propagation of cultivated strawberry.Peer reviewe

Primary medullary hemorrhage in a patient with coagulopathy due to alcoholic cirrhosis: a case report

Mild-to-moderate alcoholic cirrhosis of the liver is related to spontaneous intracerebral hemorrhage (ICH). In terms of spontaneous brainstem hemorrhage, pontine is considered as the most common site in contrast to medulla oblongata where the hemorrhage is rarely seen. This rare primary medullary hemorrhage has been attributed so far to vascular malformation (VM), anticoagulants, hypertension, hemorrhagic transformation, and other undetermined factors.Herein, we describe a 53-year-old patient with 35-year history of alcohol abuse was admitted for acute-onset isolated hemianesthesia on the right side. He was normotensive on admission. A neurological examination revealed isolated hemihypoaesthesia on the right side. He had no history of hypertension, and viral hepatitis, and nil use of anticoagulants.Brain computed tomography (CT) image demonstrated hemorrhagic lesion in dorsal and medial medulla oblongata which was ruptured into the fourth ventricle. Brain magnetic resonance imaging (MRI), and magnetic resonance angiography (MRA) demonstrated no evidence of VM. The laboratory tests implied liver dysfunction, thrombocytopenia, and coagulation disorders. Abdominal ultrasound, and CT image showed a small, and nodular liver with splenomegaly, suggestive of moderate alcoholic cirrhosis.Liver protection therapy and the management of coagulation disorders.After 14 days, he was discharged with mild hemianesthesia but with more improved parameters in laboratory tests. At the 6-month follow-up, brain MRI, MRA, and non-contrast MRI showed no significant findings except for a malacic lesion.We conclude that the patient had alcoholic cirrhosis with coagulopathy, and this may have resulted in primary medullary hemorrhage. This is a first case to report alcoholic cirrhosis as etiology of primary medullary hemorrhage

Why Do Drivers’ Collision Avoidance Maneuvers Tend to Cause SUVs to Sideslip or Rollover on Horizontal Curve and Grade Combinations?—An Analysis of the Causes Based on a Modified Multibody Dynamics Model

The extent to which drivers’ collision avoidance maneuvers affect the safety margins of sideslip and rollover is not captured by road geometric design theory. To quantify the effects of drivers’ collision avoidance maneuvers on the safety margins of sport utility vehicles (SUVs) on horizontal curve and grade combinations, a modified 8-degree-of-freedom multibody model based on SUVs was developed. The model was then used to calculate the design safety margins of sideslip and rollover for steady states and the actual safety margins for collision avoidance maneuvers. Subsequently, the design safety margin reduction rate (the difference between the design and actual safety margins divided by the design safety margin) was calculated and used to assess the safety margins. The results showed that the safety margins of SUVs were significantly reduced by braking, lane changing, and lane changing with braking. The marginal effects indicated that the greater the deceleration and the shorter the lane change duration, the greater the effect on the safety margins, particularly the sideslip safety margin. Furthermore, when the SUV was driven at 80 km·h−1 on grades with a horizontal curve radius of 270 m and 400 m, the sideslip safety margin with emergency braking (deceleration over −4.5 m·s−2) was reduced by 71% and 21%, and the rollover safety margin was reduced by 11% and 5%, respectively. Under these conditions, an emergency lane change (lane change duration less than 2 s) caused the SUV to sideslip and reduced the rollover safety margin by 47% (curve radius 270 m) and 45% (curve radius 400 m). Therefore, drivers’ collision avoidance maneuvers are a factor that cannot be neglected in alignment design

Review of Research on Road Traffic Operation Risk Prevention and Control

Road traffic safety can be ensured by preventing and controlling the potential risks in road traffic operations. The relevant literature was systematically reviewed to identify the research context and status quo in the road traffic operation risk prevention and control field and identify the key study contents needing further research. As research material, the related English and Chinese literature published between 1996 and 2021 (as of 31st December 2021) was obtained through the Web of Science Core Collection and Chinese Science Citation Database. These research materials include 22,403 English and 7876 Chinese papers. Based on the bibliometrics, this study used CiteSpace software to conduct keyword co-occurrence analysis in the field. The results show that the relevant research topics mainly covered the risks of drivers, vehicles, roads, and the traffic environment. In the aspect of driver risks, the studies focused on driving behavior characteristics. In terms of vehicle risks, the related studies were mainly about the vehicle control system, driving assistance system, hazardous material transportation, automated driving technology, safe driving speed, and vehicle collision prediction. For the road risks, the safe driving guarantee of high-risk road sections, driving risks at intersections, and safe road alignment design were the three study hotspots. In terms of traffic environment risks, identifying traffic risk locations and driving safety guarantees under adverse weather conditions were the two main research highlights. Moreover, mathematical modeling was the main method for studying road traffic operation risk. Furthermore, the impact of environmental factors on drivers, the emergency rescue system for road traffic accidents, the connection between automated driving technology and safe driving theory, and the man–machine hybrid traffic flow characteristics are the subjects needing further research

The Requirement of c-Jun N-terminal Kinase 2 in Regulation of Hypoxia-inducing Factor-1alpha mRNA Stability

The mRNAof hif-1 alpha is considered as being constitutively and ubiquitously expressed, regardless of the level of oxygen tension. However many recent reports have showed that hif-1 alpha mRNA could be regulated by natural antisense transcripts, potential microRNAs, and low O-2. In this study, it was found that a deficiency of JNK2 expression reduced HIF-1 alpha protein induction in response to nickel treatment resulting from the impaired expression of hif-1 alpha mRNA. Both the promoter luciferase assay and mRNA degradation assay clearly showed that depletion of JNK2 affected stability of hif-1 alpha mRNA, rather than regulated its transcription. In addition, nucleolin, a classic histone chaperone, was demonstrated to physically bind to hif-1 alpha mRNA and maintain its stability. Further investigation indicated that JNK2 regulated nucleolin expression and might in turn stabilize hif-1 alpha mRNA. Collectively, we provided one more piece of evidence for the oncogenic role of JNK2 and nucleolin in regulating the cancer microenvironments by controlling HIF-1 alpha expression

Monoclonal Antibodies versus Histone Deacetylase Inhibitors in Combination with Bortezomib or Lenalidomide plus Dexamethasone for the Treatment of Relapsed or Refractory Multiple Myeloma: An Indirect-Comparison Meta-Analysis of Randomized Controlled Trials

During the past decades, agents with novel mechanisms of action, such as monoclonal antibodies (MAbs) and histone deacetylase inhibitors (HDACis) have been applied to treat relapsed or refractory multiple myeloma (RRMM). The treatment outcomes of MAbs versus HDACi in combination with bortezomib or lenalidomide plus dexamethasone remain unknown. We conducted this meta-analysis to compare indirectly the efficacy and safety of MAbs and HDACis in combination with bortezomib or lenalidomide plus dexamethasone. Six trials (eight articles) were included in the meta-analysis with 3270 RRMM patients enrolled. We synthesized hazard ratios (HRs) for progression-free survival (PFS) and overall survival (OS), risk ratios (RRs) for complete response (CR),very good partial response (VGPR), overall response (OR), progressive disease plus stable disease (PD + SD) and common at least grade 3 adverse events, and their corresponding 95%confidence intervals (95% CI). Treatment with MAbs in combination with bortezomib or lenalidomide plus dexamethasone resulted in longer PFS (HR 0.83, 95% CI: 0.66–0.98), fewer incidences of at least grade 3 thrombocytopenia (RR 0.35, 95% CI: 0.23–0.53), neutropenia (RR 0.70, 95% CI: 0.51–0.96), and sense of fatigue (RR 0.37, 95% CI: 0.17–0.82) than HDACis. The daratumumab plus bortezomib or lenalidomide and dexamethasone might significantly improve PFS in comparison with HDACis plus bortezomib or lenalidomide and dexamethasone (HR 0.55, 95% CI: 0.40–0.74). In conclusion, MAbs may be superior to HDACis in achieving longer PFS and may be better tolerated when in combination therapy with bortezomib or lenalidomide plus dexamethasone

Genetic and QTL analysis of flower color and pigments in small-flowered chrysanthemum based on high-density genetic map

Flower color is an important trait for ornamental plants and is determined by pigments. Variations in the flower color of chrysanthemum are abundant, which is an important feature related to its ornamental and commercial value. However, few reports have elucidated the inheritance of the flower color and pigmentation of chrysanthemum. In this study, an F1 hybrid population, including 319 hybrids of small-flowered chrysanthemum cultivars with different flower color, was constructed. The genetic variation rules for some color and pigment-related traits were discussed based on major gene and polygene mixed inheritance analyses. Both the total anthocyanin and carotenoid contents were controlled by two pairs of additive dominant major genes and the heritability of the major genes was 70.44% and 86.03%, respectively. Based on the high-density genetic map constructed for the hybrid population, QTL analysis for the above traits was carried out using the interval mapping method. A total of 25 related QTLs and 517 closely linked markers were detected, including three QTLs controlling the total anthocyanin content and two QTLs controlling the total carotenoid content, all of which were major QTLs. Furthermore, 17 unigenes related to pigments were identified via BLAST searches with Chrysanthemum nankingense genome. For such genes, the collinearity was observed in four chromosome level genomes, including three genomes of the genus Chrysanthemum and the first genome of cultivated chrysanthemum. The results lay a foundation for in-depth exploration of flower color and pigmentation in chrysanthemum and provide a reference for future research in other ornamental plants