Bacillus velezensis LG37: Functional verification of GlnL and analysis of the ammonia assimilation metabolic pathway

High concentrations of ammonia have toxic effects on bred animals. In aquaculture, the possibility of using Bacillus preparations to remove ammonia nitrogen in aquaculture water through assimilation has been generally recognized. In this study, to analyze the metabolic pathways of ammonia assimilation in Bacillus, the characteristics and pathways of ammonia assimilation of Bacillus velezensis LG37 stored in the laboratory were analyzed. The results showed that the rate of ammonia assimilation by LG37 in a minimal medium could reach 94.2% at 20 h, and the highest assimilation rate was 95.4% within 60 h. In a minimal medium, the growth rate of LG37 in the glutamine group was significantly faster than that in the ammonia group, but the expression of glnL showed opposite results. The gene expression level of glnL in the ammonia group was significantly higher than in the glutamine group. glnL overexpression (OEglnL) and deletion (ΔglnL) strains were constructed by CRISPR/Cas9 technology and using the pHT1K vector, respectively. The expression levels of glnL in LG37, OEglnL, and ΔglnL strains were determined by RT-qPCR. The glnL gene expression levels were ordered as follows: OEglnL > LG37 > ΔglnL. In all three strains (LG37, ΔglnL, and OEglnL) and at different ammonia concentrations, the expression levels of glnL were consistent with glnA and glnB levels, showing a positive correlation. However, the expression levels of glnK and glnR in different strains hardly changed significantly

Effects of Aphid Population on the Major Economic Parameters of Soybean

During the growing period of soybeans, aphids are harmful to several economic parameters of soybean. The aphid population density has a significant positive correlation with the rate of soybean shriveling and significant negative correlation with the 100-kernel weight and individual kernel weight of soybean.Originating text in Chinese.Citation: Lin, Cunluan, Li, Lingtang, Wang, Yanpeng, Xun, Zhenshan, Zhang, Guangxin, Li, Suzhen. (1993). Effects of Aphid Population on the Major Economic Parameters of Soybean. Soybean Science, 12(3), 252-254

(2R*,6S*)-tert-Butyl 2,6-bis­(hydroxy­meth­yl)morpholine-4-carboxyl­ate

In the title compound, C11H21NO5, the H atoms of the hydr­oxy groups are disordered over two positions, each in a 1:1 ratio. In the crystal, inter­molecular O—H⋯O hydrogen bonds link pairs of mol­ecules into centrosymmetric dimers. Weak inter­molecular O—H⋯O inter­actions further link these dimers into chains extended in the [100] direction

Control Threshold of Soybean Aphids

Relationship between aphid density during flowering season and soybean yield was surveyed in 1998-1990. Based on the 3-year study, we proposed the control thresholds of soybean aphids for different levels of soybean yield.Originating text in Chinese.Citation: Lin, Cunluan, Xun, Zhenshan, Li, Lingtang, Wang, Yanpeng, Zhang, Guangxin. (1992). Control Threshold of Soybean Aphids. Soybean Science, 11(4), 318-321

The Role of Paragus quadri-fasciatus Meigen on Aphid Control and the Observations of its Biological Characteristics

Four-strip small syrphid fly, Paragus quadri-fasciatus Meigen is the important natural enemy of aphids in our region. A fly can eat about 800 aphids during its whole life. There are more than 10 kinds of aphids can be food of this fly, such as soybean aphid, Chinese sorghum aphid and radish aphid etc. The fly has 3~4 generations each year in Tonghua county, Jilin province. The adult of the first generation appears after the last ten-day period of April each year. It takes 30~35 days to complete one generation. The fly can oviposit 84~124 eggs in its whole life. Major natural enemies of the fly are ichneumon wasps, spiders, lacewings and etc.Originating text in Chinese.Citation: Gao, Junfeng, Jiang, Lianfeng, Zhang, Guangxin, Li, Chunshan, Zhao, Guangquan. (1996). The Role of Paragus quadri-fasciatus Meigen on Aphid Control and the Observations of its Biological Characteristics. Journal of Jilin Agricultural Sciences, 5(2), 60-61

Survey on the Overwintering of Syrphids in Changbai Mountains and Experiments on Artificial Protection of the Overwintering Syrphid Flies

Originating text in Chinese.Citation: Gao, Junfeng, Zhang, Guangxin, Qin, Yongchun, Yu, Kai, Li, Minghai, Qin, Yi. (1993). Survey on the Overwintering of Syrphids in Changbai Mountains and Experiments on Artificial Protection of the Overwintering Syrphid Flies. Chinese Journal of Biological Control, 9(3), 142-144

Studies on the Population Dynamics and Control Stage of Soybean Aphid in Jining, China

Soybean aphids were widespread and caused serious harm in the Jining area in recent years. About 800 aphids were found on a single soybean plant at peak time in general and even over 3,000 were found on typical individual plants. From 1988 to 1990 the aphid population was systematically investigated in soybean fields for scientific and efficient control. The relationship between population dynamics of soybean aphid and meteorological information was analyzed.Originating text in Chinese.Citation: Lin, Cunluan, Xin, Zhenshan, Li, Lintang, Zhang, Huikong, Zhang, Guangxin, Wang, Yanpeng. (1994). Studies on the Population Dynamics and Control Stage of Soybean Aphid in Jining, China. Shandong Agricultural Sciencese, 4, 44-44