172 research outputs found

    MULTIPLE DEVICE AND SENSOR BASED USER RECOGNITION

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    This document discloses a method for recognizing a user using the sum of personal information gathered from several sensors equipped in multiple devices that are accessible in a given proximity of time, space or relationship. In some instances, algorithms may be required to reconcile the discrepancies among information gathered from many sensors, and sometimes users may be informed about the discrepancies. The advantages of the method include improvement in user recognition accuracy by checking against multiple signals

    Input And Output Method Of Personalized Answers With Chatbot

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    A system and method for enabling a user to search for personal information through information systems such as search boxes or chatbots are disclosed. The system allows the user to store identified personal information, and retrieve this information at any time and from anywhere through a search box or similar application. A pre-defined input format is used to instruct the search engine so that personal information inputs are distinguishable from other common search queries. The user can retrieve the information through the usual searching techniques or by asking a question to the system and the answer is returned as a search result. The system and method are capable of instructing the information system to learn explicitly personal information of the user, and functions as a true personal assistant running on any system or device

    Ultrathin Oxide Wrapping of Plasmonic Nanoparticles via Colloidal Electrostatic Self-Assembly and their Enhanced Performances

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    Ultrathin and uniform oxide layer-wrapped plasmonic nanoparticles (NPs) have been expected in the fields of light energy conversion and optical sensing fields. In this chapter, we proposed a universal strategy to prepare such core-shell plasmonic NPs based on colloidal electrostatic attraction and self-assembly procedures. Based on the self-assembly strategy, laser ablation of metal targets in liquid medium was conducted at room temperature to one-pot fabricate the oxide-wrapped plasmonic NPs. It demonstrates that a series of core-shell nanostructured NPs such as Au@Fe2O3, Au@Al2O3, Au@CuO, Au@ZnO, Pt@TiO2, and Pd@TiO2, have been readily obtained free of contaminations. Technical analyses illustrate that those composite NPs possess uniform and symmetrical oxides layers with several nanometers in thickness. Furthermore, both the thickness and crystallinity of the oxides layer could be precisely tailored simply by controlling hydrolysis of precursors and irradiation durations. Finally, due to ultrathin wrapping of oxides, the as-obtained core-shell plasmonic NPs show excellent surface-enhanced Raman scattering (SERS) and gas-sensing performances compared with bare metal or oxides NPs

    Expression, Purification, and Characterization of Ras Protein (BmRas1) from Bombyx mori

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    The Ras subfamily is the member of small G proteins superfamily involved in cellular signal transduction. Activation of Ras signaling causes cell growth, differentiation, and survival. Bombyx mori Ras-like protein (BmRas1) may belong to the Ras subfamily. It contained an H-N-K-Ras-like domain. The BmRas1 mRNA consisted of 1459ā€‰bp. The open reading frame contained 579ā€‰bp, encoding 192 amino acids. The protein had such secondary structures as Ī±-helices, extended strand, and random coil. BmRas1 was expressed successfully in E. coli BL21. The recombinant protein was purified with metal-chelating affinity chromatography. The GTPase activity of purified protein was determined by FeSO4-(NH4)2MoO4 assay. The results showed that purified recombinant protein had intrinsic activity of GTPase. High titer polyclonal antibodies were generated by New Zealand rabbit immunized with purified protein. The gene expression features of BmRas1 at different stages and in different organs of the fifth instar larvae were analyzed by Western blot. The results showed that BmRas1 was expressed highly in three development stages including egg, pupae, and adult, but low expression in larva. BmRas1 was expressed in these tissues including head, malpighian tubule, genital gland, and silk gland. The purified recombinant protein would be utilized to further function studies of BmRas1

    Production of Exopolysaccharides from Submerged Culture of Antrodia Camphorata S-29

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    Antrodia camphorata is a unique mushroom of Taiwan, which has been used as a natural therapeutic ingredient in Traditional Chinese Medicine (TCM) for protection of diverse health related conditions. Polysaccharides produced from A. camphorata have attracted much attention of research due to cytotoxic activity and miscellaneous activities. In this paper, we report on the fermentation conditions species-speciļ¬c exopolysaccharides (EPS) from A. camphorata in submerged culture. A favorable medium for EPS production was obtained only by single-factor experiment, where Glucose and Yeast-Extracts were identified to be the most suitable carbon and nitrogen sources, with the concentration of 40 g/L and 5.0 g/L respectively. Zinc sulphate was identified to be the best salt source with the concentration of 0.4g/l. Initial pH and inoculum size for mycelial growth and EPS yield were 6.0 and 15% respectively. The maximum EPS production was 0.474 g/L in shake-flask culture, which is higher than the baseline media that was 0.351 g/L. This study provides the baseline information about production conditions for this specific specie which is crucial data to know before any further studies as it determines the properties and quantity of the desired produced specie. Keywords: A. camphorata; Exopolysaccharide; SubĀ­merged culture
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