Verteporfin mitigates sepsis-induced liver injury by blocking macrophage-derived inflammation

Purpose: To study the role of yes-associated protein (YAP) inhibition by verteporfin (VTF) in LPSinduced acute liver injury (ALI) after sepsis.Methods: In vitro, VTF was used to treat LPS-stimulated RAW 267.4 cells. In vivo, LPS was injected to induce sepsis in mice, followed by treatment with VTF. The inflammatory mediators were determined using quantitative real-time polymerase chain reaction (qRT-PCR), immunofluorescence spectroscopy (IF) and immunohistochemical staining (IHC), and the levels of YAP, P53 and ERK were measured by qRT-PCR, WB and IHC. Moreover, liver histology and liver function were examined using HE staining and ELISA respectively.Results: The results showed that VTF reduced YAP expression and inhibited LPS-induced cell activation and inflammatory cytokine production such as IL-6 and IL-1β, by attenuating the expressions of p53 and ERK pathway in macrophages. The levels of AST, AIL and TBiL remarkably decreased in ALI mice after VTF treatment (p < 0.05). Moreover, it was observed that inflammatory mediators, including inducible nitric oxide synthase (iNOS), IL-6 and IL-1β, decreased significantly in VTF treated mice (P < 0.05).Conclusion: VTF plays an antagonistic role in LPS-induced inflammatory response after ALI. Therefore, VTF is a potential medicinal agent for preventing infectious acute liver injury

Memetic Differential Evolution with an Improved Contraction Criterion

Memetic algorithms with an appropriate trade-off between the exploration and exploitation can obtain very good results in continuous optimization. In this paper, we present an improved memetic differential evolution algorithm for solving global optimization problems. The proposed approach, called memetic DE (MDE), hybridizes differential evolution (DE) with a local search (LS) operator and periodic reinitialization to balance the exploration and exploitation. A new contraction criterion, which is based on the improved maximum distance in objective space, is proposed to decide when the local search starts. The proposed algorithm is compared with six well-known evolutionary algorithms on twenty-one benchmark functions, and the experimental results are analyzed with two kinds of nonparametric statistical tests. Moreover, sensitivity analyses for parameters in MDE are also made. Experimental results have demonstrated the competitive performance of the proposed method with respect to the six compared algorithms

Compound microsatellites in complete Escherichia coli genomes

AbstractCompound microsatellites consisting of two or more repeats in close proximity have been found in eukaryotic genomes. So far such compound microsatellites have not been investigated in any prokaryotic genomes. We have therefore examined compound microsatellites in 22 complete genomes of Escherichia coli, which is one of the ideal model organisms to analyze the nature and evolution of prokaryotic compound microsatellites. Our results indicated that about 1.75–2.85% of all microsatellites could be accounted as compound microsatellites with very low complexity, and most compound microsatellites were composed of very different motifs. Compound microsatellites were significantly overrepresented in all surveyed genomes. These results were dramatically different from those in eukaryotes. We discussed the possible reasons for the observed divergence

Eag1 K +

Ether-à-go-go1 (Eag1, Kv10.1, KCNH1) K+ channel is a member of the voltage-gated K+ channel family mainly distributed in the central nervous system and cancer cells. Like other types of voltage-gated K+ channels, the EAG1 channels are regulated by a variety of endogenous signals including reactive oxygen species, rendering the EAG1 to be in the redox-regulated ion channel family. The role of EAG1 channels in tumor development and its therapeutic significance have been well established. Meanwhile, the importance of hEAG1 channels in the nervous system is now increasingly appreciated. The present review will focus on the recent progress on the channel regulation by endogenous signals and the potential functions of EAG1 channels in normal neuronal signaling as well as neurological diseases

Multiple Gravity Assist Spacecraft Trajectories Design Based on BFS and EP_DE Algorithm

The paper deals with the multiple gravity assist trajectories design. In order to improve the performance of the heuristic algorithms, such as differential evolution algorithm, in multiple gravity assist trajectories design optimization, a method combining BFS (breadth-first search) and EP DE (differential evolution algorithm based on search space exploring and principal component analysis) is proposed. In this method, firstly find the possible multiple gravity assist planet sequences with pruning based BFS and use standard differential evolution algorithm to judge the possibility of all the possible trajectories. Then select the better ones from all the possible solutions. Finally, use EP DE which will be introduced in this paper to find an optimal decision vector of spacecraft transfer time schedule (launch window and transfer duration) for each selected planet sequence. In this paper, several cases are presented to prove the efficiency of the method proposed

Reconfigurable Fabry-Pérot Cavity Antenna Basing on Phase Controllable Metasurfaces

Fabry-Pérot cavity (FPC) antenna is a kind of high-gain antenna. Compared with other high-gain antennas, such as array antenna and reflector antenna, the FPC antenna enjoys the advantages of simple structure and high efficiency. So it has attracts many attention since proposed. However, it also suffers the disadvantages of narrow band and fixed radiation patterns, due to its resonance structure. In order to overcome these disadvantages, we proposed novel strategies to realize reconfigurable FPC antennas using the phase controllable metasurfaces (MSs). Through adding PIN diodes into every unit cell of the MS, the reflection phase of the MS can be controlled by tuning the states of the diodes. Then the designed phase controllable MSs are used as the partially reflection surfaces (PRS) to realize frequency or radiation pattern reconfigurable FPC antennas. In this chapter, we analyze the basic theory of the FPC antenna and describe its radiation principle firstly. Then, reflection phase controllable MSs are designed and applied to the FPC antennas. Thus frequency and radiation pattern reconfigurable FPC antennas are formed. The design processes are described in details, and the proposed antennas are fabricated and measured. The measured results verify the correctness of the designs. Through this chapter, the readers can form a comprehensive understanding of reconfigurable FPC antenna design

Comparison of efficacy of anti-diabetics on non-diabetic NAFLD: a network meta-analysis

Objective: This study aimed to assess the efficacy of currently used anti-diabetic medications in the treatment of non-alcoholic fatty liver disease (NAFLD) without diabetes. DESIGN: The efficacy of various anti-diabetic medicines on non-alcoholic fatty liver disease in the absence of diabetes was evaluated by searching Pubmed, Embase, Cochrane Library, and Web of Science for randomized controlled trials (RCT) only. The methodological quality was evaluated using the Revised Cochrane risk-of-bias tool for randomized trials (RoB2), and the data were analyzed using Stata software (version 15.1). Results: All papers published between the time of the pooling and September 2022 were searched. There were a total of 18 randomized controlled studies with a total sample size of 1141 cases. The outcomes of interest included variations in alanine transaminase (ALT) and aspartate transaminase (AST). Rosiglitazone (SUCRA: 100%) and vildagliptin (SUCRA: 99.9%) were the best anti-diabetic medicines to improve ALT and AST, respectively, in patients with NAFLD without diabetes, according to the findings of this network meta-analysis. Conclusion: In accordance with the Network Ranking plot, Rosiglitazone was the best anti-diabetic medicine for improving ALT, and vildagliptin was the best for improving AST in patients with non-diabetic NAFLD