Reassortant between Human-Like H3N2 and Avian H5 Subtype Influenza A Viruses in Pigs: A Potential Public Health Risk

Human-like H3N2 influenza viruses have repeatedly been transmitted to domestic pigs in different regions of the world, but it is still uncertain whether any of these variants could become established in pig populations. The fact that different subtypes of influenza viruses have been detected in pigs makes them an ideal candidate for the genesis of a possible reassortant virus with both human and avian origins. However, the determination of whether pigs can act as a “mixing vessel” for a possible future pandemic virus is still pending an answer. This prompted us to gather the epidemiological information and investigate the genetic evolution of swine influenza viruses in Jilin, China.Nasopharyngeal swabs were collected from pigs with respiratory illness in Jilin province, China from July 2007 to October 2008. All samples were screened for influenza A viruses. Three H3N2 swine influenza virus isolates were analyzed genetically and phylogenetically.Influenza surveillance of pigs in Jilin province, China revealed that H3N2 influenza viruses were regularly detected from domestic pigs during 2007 to 2008. Phylogenetic analysis revealed that two distinguishable groups of H3N2 influenza viruses were present in pigs: the wholly contemporary human-like H3N2 viruses (represented by the Moscow/10/99-like sublineage) and double-reassortant viruses containing genes from contemporary human H3N2 viruses and avian H5 viruses, both co-circulating in pig populations.The present study reports for the first time the coexistence of wholly human-like H3N2 viruses and double-reassortant viruses that have emerged in pigs in Jilin, China. It provides updated information on the role of pigs in interspecies transmission and genetic reassortment of influenza viruses

Вихретоковый анизотропный термоэлектрический первичный преобразователь лучистого потока

Представлена оригинальная конструкция первичного преобразователя лучистого потока, который может служить основой для создания приемника неселективного излучения с повышенной чувствительностью

Prediction Model of Vibration Feature for Equipment Maintenance Based on Full Vector Spectrum

Establishing a prediction model is a key step for the implementation of prognostic and health management. The prediction model can be used to forecast the change trend of the characteristics of the vibration signal and analyze the potential failure in the future. Taking the vibration of power plant steam turbine as an example, the full vector fusion and fault prediction were studied. Due to the fact that the evaluation of the machine fault with only one transducer may result in a fault judgement with partiality, an information fusion method based on the theory of full vector spectrum was adopted to extract the vibration feature. An autoregressive prediction model was established. The collected vibration signals with pairing channels were fused. The time sequence of the fused vectors and spectrums were used to build the prediction model. The amplitude of main vector of rotating frequency and spectrum order structure were analyzed and predicted. The uncertainty of the spectrum structure can be eliminated by the information fusion. The reliability of the fault prediction was improved. The study on vibration prediction model system laid a technical foundation for the fault prognostic research

Development of new hepatitis E vaccines

Hepatitis E virus (HEV) infection is an emerging zoonotic disease posing a severe threat to public health in the world, especially to pregnant women. Currently, no specific treatments are available for HEV infection. Therefore, it is crucial to develop vaccine to prevent this infection. Although several potential candidate vaccines against HEV have been studied for their immunogenicity and efficacy, only Hecolin® which is developed by Xiamen Innovax Biotech Co., Ltd. and approved by China Food and Drug Administration (CFDA) in 2012, is the licensed HEV vaccine in the world so far. Extensive studies on safety, immunogenicity and efficacy in phase III clinical trials have shown that Hecolin® is a promising vaccine for HEV prevention and control. In this article, the advances on HEV vaccine development and research are briefly reviewed

Full-Vector Signal Acquisition and Information Fusion for the Fault Prediction

Fault prediction is the key technology of the predictive maintenance. Currently, researches on fault prediction are mainly focused on the evaluation of the intensities of the failure and the remaining life of the machine. There is lack of methods on the prediction of fault locations and fault characters. To satisfy the requirement of the prediction of the fault characters, the data acquisition and fusion strategies were studied. Firstly, the traditional vibration measurement mechanism and its disadvantages were presented. Then, the full-vector data acquisition and fusion model were proposed. After that, the sampling procedure and information fusion algorithm were analyzed. At last, the fault prediction method based on full-vector spectrum was proposed. The methodology is that of Dr. Bently and Dr. Muszynska. On the basis of this methodology, the application study has been carried out. The uncertainty of the spectrum structure can be eliminated by the designed data acquisition and fusion method. The reliability of the diagnosis on fault character was improved. The study on full-vector data acquisition system laid the technical foundation for the prediction and diagnosis research of the fault characters

Hypoxia Upregulates the Expression of Annexin A1 in Lung Adenocarcinoma A549 Cells

Background and objective The growth of tumor often faced up with lackness of blood and oxygen, and it has been reported that Annexin A1 may be involved in tumor. The aim of this investigation is to explore the characteristics of expression of Annexin A1 in lung adenocarcinoma A549 cells after hypoxia. Methods A549 cells were exposured to either normoxia (21%O2) or hypoxia (1%O2) condition for 4 h, 12 h, 24 h. The expressions of Annexin A1 mRNA levels were measured by RT-PCR. The expressions of Annexin 1 protein were investigaged by Western blot. The relative content of reactive oxygen species (ROS) were assayed by special kit. The expressions of nuclear translocation of NF-κB was assayed by Western blot; After been treated with ROS scavenger NAC and PDTC, the levels of Annexin 1 protein of A549 cells were measured by Western blot. Results Compared with normoxia group, the Annexin A1 mRNA in hypoxia group increased after 4 h, and then decreased gradually; Moreover, Annexin 1 protein levels of A549 cells were also increased when treated with hypoxia. An increaing of ROS production in cells exprosed to hypoxia was detected. NAC and PDTC inhibited hypoxia-induced Annexin A1 increase. Conclusion Hypoxia upregulates the expression of Annexin A1 in lung adenocarcinoma A549 cells, in which process ROS-NF-κB may paticipate in

Bacteria co-colonizing with Clostridioides difficile in two asymptomatic patients

Clostridium difficile infection (CDI) is the leading cause of nosocomial diarrhea. Co-colonization of key bacterial taxa may prevent the transition from asymptomatic C. difficile colonization to CDI. However, little is known about the composition of key bacterial taxa in asymptomatic patients