In vitro assays for bioactivity-guided isolation of antisalmonella and antioxidant compounds in Thonningia sanguinea flowers

Bioguided fractionation of the aqueous extract of Thonningia sanguinea flowers, used traditionally in the treatment of microbial diseases, led to the isolation of two phenolic compounds. The structure of these compounds was elucidated by 1H, 13C 1D NMR and mass spectrometry experiments. The antibacterial activity against Salmonella strains and antioxidant activity of the crude extract, fractions and isolated compounds was evaluated using the DPPH method. The isolated compounds identified asbrevifolin carboxylic acid and gallic acid demonstrates moderate antibacterial activity against Salmonella enteritidis, Salmonella typhimurium, and Salmonella abony. The results indicated that thetwo isolated compounds, gallic acid (IC50 = 13.5 ìM) and brevifolin carboxylic acid (IC50 = 18.0 ìM) were mainly responsible for the good scavenging activity of the aqueous extract

Detection potential of the KM3NeT detector for high-energy neutrinos from the Fermi bubbles

A recent analysis of the Fermi Large Area Telescope data provided evidence for a high-intensity emission of high-energy gamma rays with a E 2 spectrum from two large areas, spanning 50 above and below the Galactic centre (the ‘‘Fermi bubbles’’). A hadronic mechanism was proposed for this gamma-ray emission making the Fermi bubbles promising source candidates of high-energy neutrino emission. In this work Monte Carlo simulations regarding the detectability of high-energy neutrinos from the Fermi bubbles with the future multi-km3 neutrino telescope KM3NeT in the Mediterranean Sea are presented. Under the hypothesis that the gamma-ray emission is completely due to hadronic processes, the results indicate that neutrinos from the bubbles could be discovered in about one year of operation, for a neutrino spectrum with a cutoff at 100 TeV and a detector with about 6 km3 of instrumented volume. The effect of a possible lower cutoff is also considered.Published7–141.8. Osservazioni di geofisica ambientaleJCR Journalrestricte

Antibacterial activity of Thonningia sanguinea against some multi-drug resistant strains of Salmonella enterica

Background : The emergence of strains of S.enterica with multiple drug resistance (MDR) is of great concern worldwide.The extracts of flowers of Thonningia sanguinea are used in traditional medicine in Ivory Coast to treat diarrhoeal diseases including salmonellosis. Previous studies had shown inhibition of the MDR strain Salmonella Enteritidis lysotype 6. Objectives: The present study focused to investigate the effect of the extract of the flowers of Thonningia sanguinea on some clinical MDR strains of Salmonella namely S.Tyhpi, S.Typhimurium,S. Hadar and a sensitive strain (S.Enteritidis). Methods: The antimicrobial parameters were determined by double dilution with agar slant method.This method led us to determine MIC, IC 50 and MBC. Results: The MDR strain of S.Typhimurium presented the highest MIC (2.5 mg/ml) whereas the other two MDR strains (S.Hadar,S.Typhi) and the sensitive one (S.Enteritidis) had the same MIC (1.25 mg/ml).The four strains presented the same MBC (2.5 mg/ml). The MDR strain of S.Typhi is the most susceptible strain to the aqueous extract of the flowers of Thonningia sanguinea according to The IC 50values. Conclusions: The aqueous extract of Thonningia sanguinea can provide an alternative therapy for the treatment of salmonellosis, mainly for typhoid fever caused by MDR strains of S. Typhi.The extract also inhibits S.Hadar a MDR emerging strain in Ivory Coast. Keywords: Thonningia sanguinea; Salmonella, MDR strains, Ivory Coast African Health Sciences Vol. 7 (3) 2007: pp. 155-15

Antibacterial activity of Thonningia sanguinea against some multi- drug resistant strains of Salmonella enterica

Background : The emergence of strains of S.enterica with multiple drug resistance (MDR) is of great concern worldwide.The extracts of flowers of Thonningia sanguinea are used in traditional medicine in Ivory Coast to treat diarrhoeal diseases including salmonellosis. Previous studies had shown inhibition of the MDR strain Salmonella Enteritidis lysotype 6. Objectives: The present study focused to investigate the effect of the extract of the flowers of Thonningia sanguinea on some clinical MDR strains of Salmonella namely S.Tyhpi, S.Typhimurium,S. Hadar and a sensitive strain (S.Enteritidis). Methods: The antimicrobial parameters were determined by double dilution with agar slant method.This method led us to determine MIC, IC 50 and MBC. Results: The MDR strain of S.Typhimurium presented the highest MIC (2.5 mg/ml) whereas the other two MDR strains (S.Hadar,S.Typhi) and the sensitive one (S.Enteritidis) had the same MIC (1.25 mg/ml).The four strains presented the same MBC (2.5 mg/ml). The MDR strain of S.Typhi is the most susceptible strain to the aqueous extract of the flowers of Thonningia sanguinea according to The IC 50values. Conclusions: The aqueous extract of Thonningia sanguinea can provide an alternative therapy for the treatment of salmonellosis, mainly for typhoid fever caused by MDR strains of S. Typhi.The extract also inhibits S.Hadar a MDR emerging strain in Ivory Coast. Keywords: Thonningia sanguinea; Salmonella, MDR strains, Ivory Coast African Health Sciences Vol. 7 (3) 2007: pp. 155-15

The Gaia astrophysical parameters inference system (Apsis): Pre-launch description

The Gaia satellite will survey the entire celestial sphere down to 20th magnitude, obtaining astrometry, photometry, and low resolution spectrophotometry on one billion astronomical sources, plus radial velocities for over one hundred million stars. Itsmain objective is to take a census of the stellar content of our Galaxy, with the goal of revealing its formation and evolution. Gaia's unique feature is the measurement of parallaxes and proper motions with hitherto unparalleled accuracy for many objects. As a survey, the physical properties of most of these objects are unknown. Here we describe the data analysis system put together by the Gaia consortium to classify these objects and to infer their astrophysical properties using the satellite's data. This system covers single stars, (unresolved) binary stars, quasars, and galaxies, all covering a wide parameter space. Multiple methods are used for many types of stars, producing multiple results for the end user according to different models and assumptions. Prior to its application to real Gaia data the accuracy of these methods cannot be assessed definitively. But as an example of the current performance, we can attain internal accuracies (rms residuals) on F, G, K, M dwarfs and giants at G = 15 (V = 15-17) for a wide range of metallicites and interstellar extinctions of around 100 K in effective temperature (Teff), 0.1 mag in extinction (A0), 0.2 dex in metallicity ([Fe/H]), and 0.25 dex in surface gravity (log g). The accuracy is a strong function of the parameters themselves, varying by a factor of more than two up or down over this parameter range. After its launch in December 2013, Gaia will nominally observe for five years, during which the system we describe will continue to evolve in light of experience with the real data. © ESO, 2013

KM3NeT: Technical design report.

KM3NeT is a deep‐sea multidisciplinary observatory in the Mediterranean Sea that will provide innovative science opportunities spanning Astroparticle Physics and Earth and Sea Science. This is possible through the synergy created by the use of a common infrastructure allowing for long term continuous operation of a neutrino telescope and marine instrumentation. The present KM3NeT Design Study concludes with this Technical Design Report which develops the ideas put forward in the Conceptual Design Report published in April 2008