Nonparametric Change Point Diagnosis Method of Concrete Dam Crack Behavior Abnormality

The study on diagnosis method of concrete crack behavior abnormality has always been a hot spot and difficulty in the safety monitoring field of hydraulic structure. Based on the performance of concrete dam crack behavior abnormality in parametric statistical model and nonparametric statistical model, the internal relation between concrete dam crack behavior abnormality and statistical change point theory is deeply analyzed from the model structure instability of parametric statistical model and change of sequence distribution law of nonparametric statistical model. On this basis, through the reduction of change point problem, the establishment of basic nonparametric change point model, and asymptotic analysis on test method of basic change point problem, the nonparametric change point diagnosis method of concrete dam crack behavior abnormality is created in consideration of the situation that in practice concrete dam crack behavior may have more abnormality points. And the nonparametric change point diagnosis method of concrete dam crack behavior abnormality is used in the actual project, demonstrating the effectiveness and scientific reasonableness of the method established. Meanwhile, the nonparametric change point diagnosis method of concrete dam crack behavior abnormality has a complete theoretical basis and strong practicality with a broad application prospect in actual project

Transcription-associated mutation promotes RNA complexity in highly expressed genes - a major new source of selectable variation

Alternatively spliced transcript isoforms are thought to play a critical role for functional diversity. However, the mechanism generating the enormous diversity of spliced transcript isoforms remains unknown, and its biological significance remains unclear. We analyzed transcriptomes in saker falcons, chickens, and mice to show that alternative splicing occurs more frequently, yielding more isoforms, in highly expressed genes. We focused on hemoglobin in the falcon, the most abundantly expressed genes in blood, finding that alternative splicing produces 10-fold more isoforms than expected from the number of splice junctions in the genome. These isoforms were produced mainly by alternative use of de novo splice sites generated by transcription-associated mutation (TAM), not by the RNA editing mechanism normally invoked. We found that high expression of globin genes increases mutation frequencies during transcription, especially on nontranscribed DNA strands. After DNA replication, transcribed strands inherit these somatic mutations, creating de novo splice sites, and generating multiple distinct isoforms in the cell clone. Bisulfate sequencing revealed that DNA methylation may counteract this process by suppressing TAM, suggesting DNA methylation can spatially regulate RNA complexity. RNA profiling showed that falcons living on the high Qinghai–Tibetan Plateau possess greater global gene expression levels and higher diversity of mean to high abundance isoforms (reads per kilobases per million mapped reads ≥18) than their low-altitude counterparts, and we speculate that this may enhance their oxygen transport capacity under low-oxygen environments. Thus, TAM-induced RNA diversity may be physiologically significant, providing an alternative strategy in lifestyle evolution

Population transcriptomes reveal synergistic responses of DNA polymorphism and RNA expression to extreme environments on the Qinghai-Tibetan Plateau in a predatory bird

Low oxygen and temperature pose key physiological challenges for endotherms living on the Qinghai–Tibetan Plateau (QTP). Molecular adaptations to high‐altitude living have been detected in the genomes of Tibetans, their domesticated animals and a few wild species, but the contribution of transcriptional variation to altitudinal adaptation remains to be determined. Here we studied a top QTP predator, the saker falcon, and analysed how the transcriptome has become modified to cope with the stresses of hypoxia and hypothermia. Using a hierarchical design to study saker populations inhabiting grassland, steppe/desert and highland across Eurasia, we found that the QTP population is already distinct despite having colonized the Plateau <2000 years ago. Selection signals are limited at the cDNA level, but of only seventeen genes identified, three function in hypoxia and four in immune response. Our results show a significant role for RNA transcription: 50% of upregulated transcription factors were related to hypoxia responses, differentiated modules were significantly enriched for oxygen transport, and importantly, divergent EPAS1 functional variants with a refined co‐expression network were identified. Conservative gene expression and relaxed immune gene variation may further reflect adaptation to hypothermia. Our results exemplify synergistic responses between DNA polymorphism and RNA expression diversity in coping with common stresses, underpinning the successful rapid colonization of a top predator onto the QTP. Importantly, molecular mechanisms underpinning highland adaptation involve relatively few genes, but are nonetheless more complex than previously thought and involve fine‐tuned transcriptional responses and genomic adaptation

Genomic analysis of the domestication and post-Spanish conquest evolution of the llama and alpaca

Background Despite their regional economic importance and being increasingly reared globally, the origins and evolution of the llama and alpaca remain poorly understood. Here we report reference genomes for the llama, and for the guanaco and vicuña (their putative wild progenitors), compare these with the published alpaca genome, and resequence seven individuals of all four species to better understand domestication and introgression between the llama and alpaca. Results Phylogenomic analysis confirms that the llama was domesticated from the guanaco and the alpaca from the vicuña. Introgression was much higher in the alpaca genome (36%) than the llama (5%) and could be dated close to the time of the Spanish conquest, approximately 500 years ago. Introgression patterns are at their most variable on the X-chromosome of the alpaca, featuring 53 genes known to have deleterious X-linked phenotypes in humans. Strong genome-wide introgression signatures include olfactory receptor complexes into both species, hypertension resistance into alpaca, and fleece/fiber traits into llama. Genomic signatures of domestication in the llama include male reproductive traits, while in alpaca feature fleece characteristics, olfaction-related and hypoxia adaptation traits. Expression analysis of the introgressed region that is syntenic to human HSA4q21, a gene cluster previously associated with hypertension in humans under hypoxic conditions, shows a previously undocumented role for PRDM8 downregulation as a potential transcriptional regulation mechanism, analogous to that previously reported at high altitude for hypoxia-inducible factor 1α. Conclusions The unprecedented introgression signatures within both domestic camelid genomes may reflect post-conquest changes in agriculture and the breakdown of traditional management practices

Arctic introgression and chromatin regulation facilitated rapid Qinghai-Tibet Plateau colonization by an avian predator

The Qinghai-Tibet Plateau (QTP), possesses a climate as cold as that of the Arctic, and also presents uniquely low oxygen concentrations and intense ultraviolet (UV) radiation. QTP animals have adapted to these extreme conditions, but whether they obtained genetic variations from the Arctic during cold adaptation, and how genomic mutations in non-coding regions regulate gene expression under hypoxia and intense UV environment, remain largely unknown. Here, we assemble a high-quality saker falcon genome and resequence populations across Eurasia. We identify female-biased hybridization with Arctic gyrfalcons in the last glacial maximum, that endowed eastern sakers with alleles conveying larger body size and changes in fat metabolism, predisposing their QTP cold adaptation. We discover that QTP hypoxia and UV adaptations mainly involve independent changes in non-coding genomic variants. Our study highlights key roles of gene flow from Arctic relatives during QTP hypothermia adaptation, and cis-regulatory elements during hypoxic response and UV protection

Zoning Modulus Inversion Method for Concrete Dams Based on Chaos Genetic Optimization Algorithm

For dams and rock foundations of ages, the actual mechanical parameters sometimes differed from the design and the experimental values. Therefore, it is necessary to carry out the inversion analysis on main physical and mechanical parameters of dams and rock foundations. However, only the integrated deformation modulus can be inversed by utilizing the conventional inversion method, and it does not meet the actual situation. Therefore, a new method is developed in this paper to inverse the actual initial zoning deformation modulus and to determine the inversion objective function for the actual zoning deformation modulus, based on the dam displacement measured data and finite element calculation results. Furthermore, based on the chaos genetic optimization algorithm, the inversion method for zoning deformation modulus of dam, dam foundation and, reservoir basin is proposed. Combined with the project case, the feasibility and validity of the proposed method are verified

An Approach Using Adaptive Weighted Least Squares Support Vector Machines Coupled with Modified Ant Lion Optimizer for Dam Deformation Prediction

A dam deformation prediction model based on adaptive weighted least squares support vector machines (AWLSSVM) coupled with modified Ant Lion Optimization (ALO) is proposed, which can be utilized to evaluate the operational states of concrete dams. First, the Ant Lion Optimizer, a novel metaheuristic algorithm, is used to determine the punishment factor and kernel width in the least squares support vector machine (LSSVM) model, which simulates the hunting process of antlions in nature. Second, aiming to solve the premature convergence phenomenon, Levy flight is introduced into the ALO to improve the global optimization ability. Third, according to the statistical characteristics of the datum error, an improved normal distribution weighting rule is applied to update the weighted value of data samples based on the learning result of the LSSVM model. Moreover, taking a concrete arch dam in China as an example, the horizontal displacement recorded by a pendulum is used as a study object. The accuracy and validity of the proposed model are verified and evaluated based on the four evaluating criteria, and the results of the proposed model are compared with those of well-established models. The simulation results demonstrate that the proposed model outperforms other models and effectively overcomes the influence of outliers on the performance of the model. It also has high prediction accuracy, produces excellent generalization performance, and can be a promising alternative technique for the analysis and prediction of dam deformation and other fields, including flood interval prediction, the stock price market, and wind speed forecasting

Inversion Modeling of Dam-Zoning Elasticity Modulus for Heightened Concrete Dam Using ICS-IPSO Algorithm

A new approach was developed for the inversion modeling of dam-zoning elasticity modulus for heightened concrete dam, with old and new concrete zones. The proposed inversion modeling procedure takes advantage of the improved cuckoo search (ICS) algorithm and improved particle swarm optimization (IPSO) algorithm to adjust the mechanical parameters, which are used as input. An objective function is constructed based on the horizontal displacement increment by using the finite element method (FEM) and statistical analysis of the prototype monitoring data. One ideal arch dam model and one actual heightened concrete dam were taken as examples. The proposed method was used to implement the optimal selection of the dam-zoning elasticity modulus. The inversion analysis results indicate that the mechanical parameters identification method for heightened concrete gravity dams proposed in this article is accurate and has a fast convergence rate. Consequently, it can be applied as a reliable model to identify the dam-zoning elasticity modulus in practical engineering applications