795 research outputs found
Renormalization of the one-loop theory of fluctuations in polymer blends and diblock copolymer melts
Attempts to use coarse-grained molecular theories to calculate corrections to
the random-phase approximation (RPA) for correlations in polymer mixtures have
been plagued by an unwanted sensitivity to the value of an arbitrary cutoff
length, {\it i.e.}, by an ultraviolet (UV) divergence. We analyze the UV
divergence of the inverse structure factor predicted by a
`one-loop' approximation similar to that used in several previous studies. We
consider both miscible homopolymer blends and disordered diblock copolymer
melts. We show, in both cases, that all UV divergent contributions can be
absorbed into a renormalization of the values of the phenomenological
parameters of a generalized self-consistent field theory (SCFT). This
observation allows the construction of a UV convergent theory of corrections to
SCFT phenomenology. The UV-divergent one-loop contribution to are
shown to be the sum of: (i) a -independent contribution that arises from a
renormalization of the effective parameter, (ii) a -dependent
contribution that arises from a renormalization of monomer statistical segment
lengths, (iii) a contribution proportional to that arises from a
square-gradient contribution to the one-loop fluctuation free energy, and (iv)
a -dependent contribution that is inversely proportional to the degree of
polymerization, which arises from local perturbations in fluid structure near
chain ends and near junctions between blocks in block copolymers.Comment: 35 pages, 2 figure
Co-immobilization of Palm and DNase I for the development of an effective anti-infective coating for catheter surfaces
Biomaterial-associated infections, in particular, catheter-associated infections (CAI) are a major problem in clinical practice due to their ability to resist antimicrobial treatment and the host immune system. This study aimed to co-immobilize the antimicrobial lipopeptide Palm and the enzyme DNase I to introduce both antimicrobial and anti-adhesive functionalities to polydimethylsiloxane (PDMS) material, using dopamine chemistry. Surface characterization confirmed the immobilization of both compounds and no leaching of Palm from the surfaces for up to 5 days. Co-immobilization of both agents resulted in a bifunctional coating with excellent surface antimicrobial and anti-biofilm properties against both Staphylococcus aureus and Pseudomonas aeruginosa. The modified surfaces demonstrated superior biocompatibility. To better discriminate co-adhesion of both species on modified surfaces, PNA FISH (Fluorescence in situ hybridization using peptide nucleic acid probes) was employed, and results showed that P. aeruginosa was the dominant organism, with S. aureus adhering afterwards on P. aeruginosa agglomerates. Furthermore, Palm immobilization exhibited no propensity to develop bacterial resistance, as opposite to the immobilization of an antibiotic. The overall results highlighted that co-immobilization of Palm and DNase I holds great potential to be applied in the development of catheters.This study was supported by the Portuguese Foundation for
Science and Technology (FCT) under the scope of the strategic
funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-
01-0145-FEDER-006684). The authors also acknowledge the
support by FCT and the European Community fund FEDER, through
Program COMPETE, under the scope of the Projects AntiPep
PTDC/SAU-SAP/113196/2009 (FCOMP-01-0124-FEDER-016012) and
RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462) and
the PhD Grant of Diana Alves (SFRH/BD/78063/2011). This study
was also supported by the statutory fund from the Medical
University of Gdansk (Project No. 02-0087/07/508)
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The efficacy and sustainability of the CIMBAA transgenic Cry1B/Cry1C Bt cabbage and cauliflower plants for control of lepidopteran pests
In 2003 the Collaboration on Insect Management for Brassicas in Asia and Africa (CIMBAA) public/private partnership selected the Cry1B/Cry1C Bt protein combination as having the potential to provide effective and sustainable control of diamondback moth (DBM), Plutella xylostella. Following transformations and extensive plant selection, insect efficacy trials were undertaken in 2008 to 2010 in north India (Murthal near New Delhi) and south India (near Bengaluru) in large scale screen-house experiments using artificial infestations on the best performing (Elite Event) plant lines and on hybrids produced from them. Plant damage was scored on a scale of 0 (no visible damage) to 4 (plant effectively destroyed). For DBM, cabbage cluster caterpillar (Crocidolomia binotalis), cabbage webworm (Hellula undalis) and semi-looper (Trichoplusia ni) there was zero insect survival and a zero damage score on the Elite Event lines and on their hybrids, while control plants had 50 to 100% insect survival (depending on species, life stage and trials) and damage scores of 3.3 to 4. Cabbage white (Pieris brassicae) and common army worm (Spodoptera litura) showed some larval survival and damage scores up to 1.4 (especially in early trials) but no survival to pupation. Screening of DBM populations worldwide (inc. 18 populations for Cry1B and 13 for Cry1C from India) showed mean LC50s close to that of international susceptible strains. To date F2 screening has not identified the presence of resistance genes in DBM in the field. Cry1B resistance was slowly developed artificially in the laboratory but 1C resistance and resistance to the Cry1B/1C combination was harder to develop and had higher fitness costs. The ‘resistant’ lines showed some extended survival of stunted DBM larvae on dual gene Bt plants but no survival to pupation. There was no cross-resistance between Cry1B and Cry1C. Resistance to both genes was autosomal and recessive. Beneficial insects were demonstrated to have the potential to provide additional mortality on rare surviving insects in Bt fields. Aphids were well controlled for the first 40 days post-transplanting using imidacloprid pelleted onto seed and, if necessary, by 1-2 Verticillium lecanii sprays thereafter. Surviving S. litura and Helicoverpa armigera in Bt sprayed fields were well controlled by one or two application
Confocal laser scanning microscopy as a valuable tool in Diptera larval morphology studies
Larval morphology of flies is traditionally studied using light microscopy, yet in the case of fine structures compound light microscopy is limited due to problems of resolution, illumination and depth of field, not allowing for precise recognition of sclerites’ edges and interactions. Using larval instars of cyclorrhaphan Diptera, we show the usefulness of confocal laser scanning microscopy (CLSM) for studying the morphological characters of immature stages by taking advantage of the autofluorescent properties of cephaloskeleton structures. We compare data obtained from killed but unprepared larvae with those from larvae prepared by clearing according to two commonly used methods, either with potassium hydroxide or with Hoyer’s medium. We also evaluated the CLSM application for examining already slide-mounted larvae stored in museum collections and those freshly prepared. Our results indicate that CLSM and 3D reconstruction are excellent for visualizing small, compound structures of cylrorrhaphan larvae cephaloskeleton, if appropriate clearing techniques, i.e. the application of KOH, are used. Maximum intensity projection of confocal data sets obtained from material freshly prepared and that stored in museum collection does not differ. Because of this and the fact that KOH is commonly used as a clearing method to examine the cephaloskeleton of Diptera larvae, it is possible, and highly recommended, to use slides already prepared with this method for re-examination by CLSM. We conclude that CLSM application can be an invaluable source of data for studies of larval morphology of Cyclorrhapha by way of taxonomic diagnoses, character identification and improvement in characters homologization.This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited
On-Farm IPM Of Chickpea In Nepal Proceedings of the International Workshop on Planning and Implementation of On-farm Chickpea IPM in Nepal 6-7 September 2000
Chickpea is a traditional crop, and is an important component in the daily diet of
the Nepalese. It is one of the major grain legume crops grown as a sole or mixed
crop in the rice- and maize-based cropping systems in Nepal. Area under chickpea
has shown a decreasing trend for the last two decades, as a result of increasing
incidence of diseases (botrytis gray mold) and insects (pod borer). Additionally
abiotic constraints have also been identified, causing low and unstable yields at the
national level. Consequences of decreased chickpea cultivation in Nepal include
reduced opportunities for ameliorative effects of legumes on cropping system and
sustainability, and decreased local accessibility of chickpea as a nutritious dietary
component, particularly for poor sections of the community.
Scientists from Nepal Agricultural Research Council (NARC), International
Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Natural Resources
Institute (NRI), non-governmental organizations, and farmers (women and men)
participated in the meeting. The present status of various components of on-farm
integrated pest management (IPM) in Nepal were discussed and accounts of
current research on IPM in different institutions were presented. Good progress has
been made and prospects of continued collaborative research and development on
IPM are encouraging. Site specific work plans and role of partners with the funding
for the period of three years (2000-03) from the Crop Protection Programme (CPP)
of the Department for International Development (DFID), UK were finalized. High
priority was given to participatory on-farm validation and scale-up of the available
components of IPM of botrytis gray mold and pod borer and their integration with
other improved agronomical practices for sustainable chickpea production in
Nepal
Alternative pest control approaches NPV for pod borer control and its uptake in Nepal
Pod borer is the most serious pest of chickpea in Nepal and its control based upon chemical insecticides alone has met major problems of resistance. There is a clear need for alternative pod borer control techniques. One of the most promising alternative controls i nucleopolyhedrovirus or NPV This is effective, safe and has bee adopted in a number of countries as part of the national pod bore IPM strategy. The chickpea IPM project has conducted evaluation of NPV in Nepal and results show it to be as effective or better than existing chemical control. However, if promotion of NPV in Nepal for pod borer control were to be adopted, a policy for the supply of NPV would need to be developed. Importation is feasible but local production would probably be cheaper. Several models of local production exist including farmer production, village production, state or extension service production and commercial private sector production and these models need to be evaluated for adoption in Nepal. A national system of regulation for NPV would also need to be developed
Orbital dependent nucleonic pairing in the lightest known isotopes of tin
By studying the 109Xe-->105Te-->101Sn superallowed alpha-decay chain, we
observe low-lying states in 101Sn, the one-neutron system outside doubly magic
100Sn. We find that the spins of the ground state (J = 7=2) and first excited
state (J = 5=2) in 101Sn are reversed with respect to the traditional level
ordering postulated for 103Sn and the heavier tin isotopes. Through simple
arguments and state-of-the-art shell model calculations we explain this
unexpected switch in terms of a transition from the single-particle regime to
the collective mode in which orbital-dependent pairing correlations, dominate.Comment: 5 pages 3 figure
Development of the (d,n) proton-transfer reaction in inverse kinematics for structure studies
Transfer reactions have provided exciting opportunities to study the
structure of exotic nuclei and are often used to inform studies relating to
nucleosynthesis and applications. In order to benefit from these reactions and
their application to rare ion beams (RIBs) it is necessary to develop the tools
and techniques to perform and analyze the data from reactions performed in
inverse kinematics, that is with targets of light nuclei and heavier beams. We
are continuing to expand the transfer reaction toolbox in preparation for the
next generation of facilities, such as the Facility for Rare Ion Beams (FRIB),
which is scheduled for completion in 2022. An important step in this process is
to perform the (d,n) reaction in inverse kinematics, with analyses that include
Q-value spectra and differential cross sections. In this way, proton-transfer
reactions can be placed on the same level as the more commonly used
neutron-transfer reactions, such as (d,p), (9Be,8Be), and (13C,12C). Here we
present an overview of the techniques used in (d,p) and (d,n), and some recent
data from (d,n) reactions in inverse kinematics using stable beams of 12C and
16O.Comment: 9 pages, 4 figures, presented at the XXXV Mazurian Lakes Conference
on Physics, Piaski, Polan
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