3,481 research outputs found
Analysis of Oct4-dependent transcriptional networks regulating self-renewal and pluripotency in human embryonic stem cells
The POU domain transcription factor OCT4 is a key regulator of pluripotency in the early mammalian embryo and is highly expressed in the inner cell mass of the blastocyst. Consistent with its essential role in maintaining pluripotency, Oct4 expression is rapidly downregulated during formation of the trophoblast lineage. To enhance our understanding of the molecular basis of this differentiation event in humans, we used a functional genomics approach involving RNA interference-mediated suppression of OCT4 function in a human ESC line and analysis of the resulting transcriptional profiles to identify OCT4-dependent genes in human cells. We detected altered expression of >1,000 genes, including targets regulated directly by OCT4 either positively (NANOG, SOX2, REX1, LEFTB, LEFTA/EBAF DPPA4, THY1, and TDGF1) or negatively (CDX2, EOMES, BMP4, TBX18, Brachyury [T], DKK1, HLX1, GATA6, ID2, and DLX5), as well as targets for the OCT4-associated stem cell regulators SOX2 and NANOG. Our data set includes regulators of ACTIVIN, BMP, fibroblast growth factor, and WNT signaling. These pathways are implicated in regulating human ESC differentiation and therefore further validate the results of our analysis. In addition, we identified a number of differentially expressed genes that are involved in epigenetics, chromatin remodeling, apoptosis, and metabolism that may point to underlying molecular mechanisms that regulate pluripotency and trophoblast differentiation in humans. Significant concordance between this data set and previous comparisons between inner cell mass and trophectoderm in human embryos indicates that the study of human ESC differentiation in vitro represents a useful model of early embryonic differentiation in humans
An atom fiber for guiding cold neutral atoms
We present an omnidirectional matter wave guide on an atom chip. The
rotational symmetry of the guide is maintained by a combination of two current
carrying wires and a bias field pointing perpendicular to the chip surface. We
demonstrate guiding of thermal atoms around more than two complete turns along
a spiral shaped 25mm long curved path (curve radii down to 200m) at
various atom--surface distances (35-450m). An extension of the scheme for
the guiding of Bose-Einstein condensates is outlined
A Proper Motion Survey for White Dwarfs with the Wide Field Planetary Camera 2
We have performed a search for halo white dwarfs as high proper motion
objects in a second epoch WFPC2 image of the Groth-Westphal strip. We identify
24 high proper motion objects with mu > 0.014 ''/yr. Five of these high proper
motion objects are identified as strong white dwarf candidates on the basis of
their position in a reduced proper motion diagram. We create a model of the
Milky Way thin disk, thick disk and stellar halo and find that this sample of
white dwarfs is clearly an excess above the < 2 detections expected from these
known stellar populations. The origin of the excess signal is less clear.
Possibly, the excess cannot be explained without invoking a fourth galactic
component: a white dwarf dark halo. We present a statistical separation of our
sample into the four components and estimate the corresponding local white
dwarf densities using only the directly observable variables, V, V-I, and mu.
For all Galactic models explored, our sample separates into about 3 disk white
dwarfs and 2 halo white dwarfs. However, the further subdivision into the thin
and thick disk and the stellar and dark halo, and the subsequent calculation of
the local densities are sensitive to the input parameters of our model for each
Galactic component. Using the lowest mean mass model for the dark halo we find
a 7% white dwarf halo and six times the canonical value for the thin disk white
dwarf density (at marginal statistical significance), but possible systematic
errors due to uncertainty in the model parameters likely dominate these
statistical error bars. The white dwarf halo can be reduced to around 1.5% of
the halo dark matter by changing the initial mass function slightly. The local
thin disk white dwarf density in our solution can be made consistent with the
canonical value by assuming a larger thin disk scaleheight of 500 pc.Comment: revised version, accepted by ApJ, results unchanged, discussion
expande
A Morphological and Multicolor Survey for Faint QSOs in the Groth-Westphal Strip
Quasars representative of the populous faint end of the luminosity function
are frustratingly dim with m~24 at intermediate redshift; moreover groundbased
surveys for such faint QSOs suffer substantial morphological contamination by
compact galaxies having similar colors. In order to establish a more reliable
ultrafaint QSO sample, we used the APO 3.5-m telescope to take deep groundbased
U-band CCD images in fields previously imaged in V,I with WFPC2/HST. Our
approach hence combines multicolor photometry with the 0.1" spatial resolution
of HST, to establish a morphological and multicolor survey for QSOs extending
about 2 magnitudes fainter than most extant groundbased surveys. We present
results for the "Groth-Westphal Strip", in which we identify 10 high likelihood
UV-excess candidates having stellar or stellar-nucleus+galaxy morphology in
WFPC2. For m(606)<24.0 (roughly B<24.5) the surface density of such QSO
candidates is 420 (+180,-130) per square degree, or a surface density of 290
(+160,-110) per square degree with an additional V-I cut that may further
exclude compact emission line galaxies. Even pending confirming spectroscopy,
the observed surface density of QSO candidates is already low enough to yield
interesting comparisons: our measures agree extremely well with the predictions
of several recent luminosity function models.Comment: 29 pages including 6 tables and 7 figures. As accepted for
publication in The Astronomical Journal (minor revisions
Kritik der Strumaepidemiologie: III. Vergleich von Röntgen-Thoraxaufnahmen, Palpation und sonographischer Volumetrie
Disorder-Induced Multiple Transition involving Z2 Topological Insulator
Effects of disorder on two-dimensional Z2 topological insulator are studied
numerically by the transfer matrix method. Based on the scaling analysis, the
phase diagram is derived for a model of HgTe quantum well as a function of
disorder strength and magnitude of the energy gap. In the presence of sz
non-conserving spin-orbit coupling, a finite metallic region is found that
partitions the two topologically distinct insulating phases. As disorder
increases, a narrow-gap topologically trivial insulator undergoes a series of
transitions; first to metal, second to topological insulator, third to metal,
and finally back to trivial insulator. We show that this multiple transition is
a consequence of two disorder effects; renormalization of the band gap, and
Anderson localization. The metallic region found in the scaling analysis
corresponds roughly to the region of finite density of states at the Fermi
level evaluated in the self-consistent Born approximation.Comment: 5 pages, 5 figure
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