Оценка внутрисортового полиморфизма генов устойчивости к грибным болезням томата у сортов селекции Мичуринского ГАУ

Relevance. The studies are aimed at assessing the intravarietal allelic diversity of genes for resistance to fusariosis and cladosporiosis of tomato cultivars bred at the Michurinsky State Agrarian University using molecular markers.Methods. The biological objects of the study are tomato varieties bred at the Michurinsky State Agrarian University. A total of 10 varieties were analyzed. The assessment of intravarietal polymorphism was determined on a sample of 10 plants of each variety. DNA extraction was performed using a Quick-DNA Plant/Seed Miniprep Kit (Zymo Research, USA) for DNA extraction according to the manufacturer's protocol. The P7 DNA marker was used to identify the cladosporosis resistance gene Cf-19. The presence of the fusarium wilt resistance gene I-2 was determined using the marker I-2/5. Amplification results were visualized by electrophoresis in 2% agarose gel.Results. 10 plants of 10 varieties of Michurinsky GAU selection tomato were analyzed using molecular markers of genes for resistance to cladosporiosis and fusariosis. An assessment of intravarietal polymorphism was carried out. In most of the varieties studied, the genes analyzed are identified in a heterozygous state. Three varieties (Vivat, Carotinka, Krasavets) containing only alleles of susceptibility to the causative agent of the disease in the genotype I-2 identified by the gene. Individual samples were homozygous for the fusariosis resistance allele. Analysis of the gene for Cf-19 resistance to cladosporiosis showed that most of the varieties studied are heterozygous forms. In some varieties, all ten plants were heterozygous. These include Krasavets, Vivat, Orlik, Bui Tour. The remaining samples had different allelic compositions. The pathogen resistance allele has been identified in plants of the Nepryadva and Chernysh varieties. The dominant allele in the homozygous state was detected in the Japanese variety. At the same time, all the analyzed plants of this variety were monotonous.Conclusion. It has been established, but a significant part of the varieties of tomato selection of Michurinsky GAU is polymorphic in the genes for resistance to cladosporiosis and fusariosis. In this case, a significant part of the varieties can be used in selection during preliminary molecular analysis. According to the gene for resistance to fusariosis, the following varieties are identified sources of the resistance allele: Sokol, Nepryadva, Yaponchik, Orlik, Zolotnichok, Chernysh. In them, the gene is I-2 represented in a heterozygous state. Intraortic polymorphism was also noted for the Cf-19 gene. Most samples have two alleles. The exception is the Japanese variety, which is the dominant homozygote for this gene. Цель. Исследования направлены на оценку внутрисортового аллельного разнообразия генов устойчивости к фузариозу и кладоспориозу томата сортов селекции Мичуринского ГАУ с использованием молекулярных маркеров.Методология. Биологическими объектами исследования являются сорта томата селекции Мичуринского ГАУ. Всего проанализировано 10 сортов. Оценка внутрисортового полиморфизма определялась на выборке из 10 растений каждого сорта. Экстрагирование ДНК было проведено с использованием набора для экстрагирования ДНК Quick-DNA Plant/Seed Miniprep Kit (Zymo Research, USA) согласно протоколу производителя. Для идентификации гена устойчивости к кладоспоризу Cf19 использовали ДНК-маркер Р7. Наличие гена устойчивости к фузариозному увяданию I-2 определяли с помощью маркера I-2/5. Визуализацию результатов амплификации осуществляли с помощью электрофореза в 2% агарозном геле.Результаты. Было проанализировано по 10 растений 10 сортов томата селекции Мичуринского ГАУ с использованием молекулярных маркеров генов устойчивости к кладоспориозу и фузариозу. Проведена оценка внутрисортового полиморфизма. У большинства исследуемых сортов анализируемые гены идентифицированы в гетерозиготном состоянии. По гену I-2 выявлены три сорта (Виват, Каротинка, Красавец), содержащих в генотипе только аллели восприимчивости к возбудителю заболевания. Отмечены отдельные образцы гомозиготные по аллелю устойчивости к фузариозу. Анализ гена Cf-19 устойчивости к кладоспориозу показал, что большинство исследуемых сортов являются гетерозиготными формами. У части сортов все десять растений были гетерозиготны. К ним относятся Красавец, Виват, Орлик, Буй-Тур. Остальные образцы имели различный аллельный состав. Аллель устойчивости к возбудителю был идентифицирован у растений сорта Непрядва и Черныш. Доминантный аллель в гомозиготном состоянии выявлен у сорта Япончик. При этом все анализируемые растения этого сорта были однообразны.Заключение. Установлено, что значительная часть сортов томата селекции Мичуринского ГАУ является полиморфной по генам устойчивости к кладоспориозу и фузариозу. При этом значительная часть сортов может быть использована в селекции при проведении предварительного молекулярного анализа. По гену устойчивости к фузариозу выделены следующие сорта источники аллеля устойчивости: Сокол, Непрядва, Япончик, Орлик, Золотничок, Черныш. У них ген I-2 представлен в гетерозиготном состоянии. Внутрисортовой полиморфизм отмечен и для гена Cf-19. Большинство образцов имеют два аллеля. Исключение составляет сорт Япончик, который является доминантной гомозиготой по данному гену.

Desmoglein 3, via an Interaction with E-cadherin, Is Associated with Activation of Src

Desmoglein 3 (Dsg3), a desmosomal adhesion protein, is expressed in basal and immediate suprabasal layers of skin and across the entire stratified squamous epithelium of oral mucosa. However, increasing evidence suggests that the role of Dsg3 may involve more than just cell-cell adhesion.To determine possible additional roles of Dsg3 during epithelial cell adhesion we used overexpression of full-length human Dsg3 cDNA, and RNAi-mediated knockdown of this molecule in various epithelial cell types. Overexpression of Dsg3 resulted in a reduced level of E-cadherin but a colocalisation with the E-cadherin-catenin complex of the adherens junctions. Concomitantly these transfected cells exhibited marked migratory capacity and the formation of filopodial protrusions. These latter events are consistent with Src activation and, indeed, Src-specific inhibition reversed these phenotypes. Moreover Dsg3 knockdown, which also reversed the decreased level of E-cadherin, partially blocked Src phosphorylation.Our data are consistent with the possibility that Dsg3, as an up-stream regulator of Src activity, helps regulate adherens junction formation

Plakophilin-3 Is Required for Late Embryonic Amphibian Development, Exhibiting Roles in Ectodermal and Neural Tissues

The p120-catenin family has undergone a significant expansion during the evolution of vertebrates, resulting in varied functions that have yet to be discerned or fully characterized. Likewise, members of the plakophilins, a related catenin subfamily, are found throughout the cell with little known about their functions outside the desmosomal plaque. While the plakophilin-3 (Pkp3) knockout mouse resulted in skin defects, we find larger, including lethal effects following its depletion in Xenopus. Pkp3, unlike some other characterized catenins in amphibians, does not have significant maternal deposits of mRNA. However, during embryogenesis, two Pkp3 protein products whose temporal expression is partially complimentary become expressed. Only the smaller of these products is found in adult Xenopus tissues, with an expression pattern exhibiting distinctions as well as overlaps with those observed in mammalian studies. We determined that Xenopus Pkp3 depletion causes a skin fragility phenotype in keeping with the mouse knockout, but more novel, Xenopus tailbud embryos are hyposensitive to touch even in embryos lacking outward discernable phenotypes, and we additionally resolved disruptions in certain peripheral neural structures, altered establishment and migration of neural crest, and defects in ectodermal multiciliated cells. The use of two distinct morpholinos, as well as rescue approaches, indicated the specificity of these effects. Our results point to the requirement of Pkp3 in amphibian embryogenesis, with functional roles in a number of tissue types

Immunoglobulin, glucocorticoid, or combination therapy for multisystem inflammatory syndrome in children: a propensity-weighted cohort study

Background: Multisystem inflammatory syndrome in children (MIS-C), a hyperinflammatory condition associated with SARS-CoV-2 infection, has emerged as a serious illness in children worldwide. Immunoglobulin or glucocorticoids, or both, are currently recommended treatments. Methods: The Best Available Treatment Study evaluated immunomodulatory treatments for MIS-C in an international observational cohort. Analysis of the first 614 patients was previously reported. In this propensity-weighted cohort study, clinical and outcome data from children with suspected or proven MIS-C were collected onto a web-based Research Electronic Data Capture database. After excluding neonates and incomplete or duplicate records, inverse probability weighting was used to compare primary treatments with intravenous immunoglobulin, intravenous immunoglobulin plus glucocorticoids, or glucocorticoids alone, using intravenous immunoglobulin as the reference treatment. Primary outcomes were a composite of inotropic or ventilator support from the second day after treatment initiation, or death, and time to improvement on an ordinal clinical severity scale. Secondary outcomes included treatment escalation, clinical deterioration, fever, and coronary artery aneurysm occurrence and resolution. This study is registered with the ISRCTN registry, ISRCTN69546370. Findings: We enrolled 2101 children (aged 0 months to 19 years) with clinically diagnosed MIS-C from 39 countries between June 14, 2020, and April 25, 2022, and, following exclusions, 2009 patients were included for analysis (median age 8·0 years [IQR 4·2–11·4], 1191 [59·3%] male and 818 [40·7%] female, and 825 [41·1%] White). 680 (33·8%) patients received primary treatment with intravenous immunoglobulin, 698 (34·7%) with intravenous immunoglobulin plus glucocorticoids, 487 (24·2%) with glucocorticoids alone; 59 (2·9%) patients received other combinations, including biologicals, and 85 (4·2%) patients received no immunomodulators. There were no significant differences between treatments for primary outcomes for the 1586 patients with complete baseline and outcome data that were considered for primary analysis. Adjusted odds ratios for ventilation, inotropic support, or death were 1·09 (95% CI 0·75–1·58; corrected p value=1·00) for intravenous immunoglobulin plus glucocorticoids and 0·93 (0·58–1·47; corrected p value=1·00) for glucocorticoids alone, versus intravenous immunoglobulin alone. Adjusted average hazard ratios for time to improvement were 1·04 (95% CI 0·91–1·20; corrected p value=1·00) for intravenous immunoglobulin plus glucocorticoids, and 0·84 (0·70–1·00; corrected p value=0·22) for glucocorticoids alone, versus intravenous immunoglobulin alone. Treatment escalation was less frequent for intravenous immunoglobulin plus glucocorticoids (OR 0·15 [95% CI 0·11–0·20]; p<0·0001) and glucocorticoids alone (0·68 [0·50–0·93]; p=0·014) versus intravenous immunoglobulin alone. Persistent fever (from day 2 onward) was less common with intravenous immunoglobulin plus glucocorticoids compared with either intravenous immunoglobulin alone (OR 0·50 [95% CI 0·38–0·67]; p<0·0001) or glucocorticoids alone (0·63 [0·45–0·88]; p=0·0058). Coronary artery aneurysm occurrence and resolution did not differ significantly between treatment groups. Interpretation: Recovery rates, including occurrence and resolution of coronary artery aneurysms, were similar for primary treatment with intravenous immunoglobulin when compared to glucocorticoids or intravenous immunoglobulin plus glucocorticoids. Initial treatment with glucocorticoids appears to be a safe alternative to immunoglobulin or combined therapy, and might be advantageous in view of the cost and limited availability of intravenous immunoglobulin in many countries. Funding: Imperial College London, the European Union's Horizon 2020, Wellcome Trust, the Medical Research Foundation, UK National Institute for Health and Care Research, and National Institutes of Health

P-cadherin expression in breast cancer: a review

P-cadherin is frequently over-expressed in high-grade invasive breast carcinomas and has been reported to be an enhancer of migration and invasion of breast cancer cells, being correlated with tumour aggressiveness. In addition, expression of P-cadherin is well established as an indicator of poor prognosis in human breast cancer, which has stimulated our interest in studying its role in this setting. This review describes the most important findings on P-cadherin expression and function in normal mammary tissue and breast cancer cells, emphasizing that further research is required to elucidate the role played by this protein in human mammary tumours

Basic principles of enteral feeding in premature infants

То optimize the feeding of preterm infants is now one of the priority areas in neonatology and pediatrics because neonatal malnutrition has a negative impact on the health and development of a preterm infant, including on those at a later age. Current recommendations for the enteral feeding of preterm infants are based on the knowledge of their anatomic and physiological features and the need to prevent a menacing disease, such as necrotizing enterocolitis. The feeding of preterm infants at any gestational age with their mother's native breast milk is most optimal. Recommendations for breast milk enrichment are warranted for preterm infants with higher nutrient requirements. The paper presents the differential algorithms and regimens for the feeding of preterm infants with both breast milk and milk formulas depending on many factors, such as gestational age, birth weight, and clinical status

OPEN BORDERS OF THE EUROPEAN UNION: NATIONAL AND EUROPEAN IDENTITY ON THE EXAMPLE OF CERTAIN EU MEMBER-STATES

During the acute phase of the migration crisis (2014–2015) in a large number of European Union states, policies restricting internal borders and the strengthening of external borders took place, which raised serious questions about the future of the European Union’s borders. Ever since the emergence of supranational structures, freedom of movement, capital, goods, services and labor has all been the principal focus points of the EU countries cooperation. The uncertain nature and complexlexity regarding migration issues, strengthening of Eurosceptic sentiments in certain EU countries, the commitment to population in certain member states, the EU’s future and its main achievements (including such issues as freedom of borders, which is still under threat of illegal migration and criminal-terrorist elements) remain a big question.The authors of the article aim to evaluate the current state and threats to open borders of the European Union using analysis of common European identity. One of its elements includes the population’s attitude to issues such as the mobility and freedom of borders and their relation with national identity on the example of different EU countries, which consistently support and/or criticize the existing supranational model.In the first part of the article, the author discuss’ the main causes for why the EU borders at present have grown so important. Also, their correlation to the task of strengthening solidarity and trust throughout the EU, by overcoming a common European identity crisis.In the second part of the article the process of forming a common European identity and the reflection of this concept in the basic documents of the EU are presented retrospectively. In the third part the place of migration and its relation to the problem of borders in national and European identities through the example of EU member-states is illustrated by the example of countries whose population is both European oriented and by Eurosceptic states. In the final section conclusions are drawn regarding the importance of «open borders» for the EU in medium and long term outlook

The interdisciplinary fusion of engineering education by forming at students of technical institutes of higher education the ability to innovation of engineering activity

In the offered work interdisciplinary integration is described as one of the main characteristics of innovative engineering action, its definition is stated more precisely and concept «ability to innovative engineering action» is formulated. The model of methodical system of forming the ability to innovate engineering action among students of technical faculties, developed on the basis of interdisciplinary integration, is offered.В статье описывается междисциплинарная интеграция как одна из основных характеристик инновационной инженерной деятельности, уточняется ее определение и формулируется понятие «способность к инновационной инженерной деятельности». Предлагается модель методической системы формирования у студентов технических вузов способности к инновационной инженерной деятельности, разработанная на основе междисциплинарной интеграции