2 research outputs found
Application of microsatellite markers for breeding and genetic conservation of herds of Pantaneiro sheep
Background: The aim of the present study was to assess the genetic
diversity of Pantaneiro sheep, using microsatellite markers, in order
to assist maintenance and management plans, enhance mating systems and
reduce the inbreeding rate. A total of 127 animals were genotyped at
eight microsatellite loci. They belonged to populations from the
Experimental Farm of the Universidade Federal da Grande Dourados (UFGD)
(Dourados/MS/Brazil) and Embrapa Pantanal (Corumb\ue1/MS/Brazil).
Results: The population of Pantaneiro sheep fromthe UFGD exhibited a
highmean number of alleles (11.13) and allelic richness (10.66). The
polymorphic information content was highly informative in the locus
studied, resulting in a mean value of 0.71. Observed heterozygosity was
lower than expected for all molecular markers assessed. The analysis of
molecular variance showed a differentiation rate of 5.2% between
populations. Conclusions: The results of the statistical parameters
indicated that populations of Pantaneiro sheep require special
attention on herd management, and it's further necessary to implement
breeder exchange programs in order to preserve the genetic variability
of these populations. Furthermore, the maintenance of those populations
in their typical habitats is rather required to allow different
responses from the herds to the interactions between genotype and
environment
Genetic identification of interspecific hybrid of Neotropical catfish species (Pseudoplatystoma corruscans vs. Pseudoplatystoma reticulatum) in rivers of Mato Grosso do Sul State, Brazil
Hybrids of the species Pseudoplatystoma corruscans vs. Pseudoplatystoma reticulatum are extensively traded because they are more docile and precocious compared to their parents. This study aimed to identify the occurrence of hybrid 'Surubim'in rivers of the State of Mato Grosso do Sul, applying molecular techniques. Pseudoplatystoma corruscans, Pseudoplatystoma reticulatum and hybrid 'Surubim'were detected using multiplex PCR and PCR-RFLP in RAG2, GLOBIN, EF1 alpha, 18S rRNA nuclear genes, and mitochondrial 16S rRNA gene. The results revealed the presence of F-1 hybrids in rivers and the occurrence of genetic introgression, evidenced by the Post-F-1 hybrids. Furthermore, discrepancies between the morphological and genetic identification were observed, demonstrating the effectiveness of using different molecular markers for the correct identification of these hybrids. The necessity of implementation of management and conservation projects to maintain the genetic integrity of native species populations threatened by hybridization in the rivers of Mato Grosso do Sul State is evident