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Isorhodopsin: an undervalued visual pigment analog

Solid state NMR/Biophysical Organic Chemistr

Coupled HOOP signature correlates with quantum yield of isorhodopsin and analog pigments

Bio-organic Synthesi

Retinal-Based Proton Pumping in the Near Infrared

Solid state NMR/Biophysical Organic Chemistr

The retina of Spalax ehrenbergi: Novel histologic features supportive of a modified photosensory role

FWN – Publicaties zonder aanstelling Universiteit Leide

Optical switching between long-lived states of opsin transmembrane voltage sensors

Opsin-based transmembrane voltage sensors (OTVSs) are membrane proteins increasingly used in optogenetic applications to measure voltage changes across cellular membranes. In order to better understand the photophysical properties of OTVSs, we used a combination of UV-Vis absorption, fluorescence and FT-Raman spectroscopy to characterize QuasAr2 and NovArch, two closely related mutants derived from the proton pump archaerhodopsin-3 (AR3). We find both QuasAr2 and NovArch can be optically cycled repeatedly between O-like and M-like states using 5-min exposure to red (660 nm) and near-UV (405 nm) light. Longer red-light exposure resulted in the formation of a long-lived photoproduct similar to pink membrane, previously found to be a photoproduct of the BR O intermediate with a 9-cis retinylidene chromophore configuration. However, unlike QuasAr2 whose O-like state is stable in the dark, NovArch exhibits an O-like state which slowly partially decays in the dark to a stable M-like form with a deprotonated Schiff base and a 13-cis,15-anti retinylidene chromophore configuration. These results reveal a previously unknown complexity in the photochemistry of OTVSs including the ability to optically switch between different long-lived states. The possible molecular basis of these newly discovered properties along with potential optogenetic and biotechnological applications are discussed.Solid state NMR/Biophysical Organic Chemistr

Expression and Spectroscopic Characterization of Melanopsin and Squid Rhodopsin

FWN – Publicaties zonder aanstelling Universiteit Leide