Leishmania mexicana amazonensis isolated from a patient with fatal mucosal leishmaniasis

Faculdade de Medicina (FMD

Leishmanioses tegumentares: aspectos clínicos e imunopatológicos Cutaneous leishmaniasis: clinical and immunopathological aspects

Foi visto nesta revisão que os parasitas do gênero Leishmania induzem uma variedade de respostas complexas nos hospedeiros vertebrados, efetuadas e/ou moduladas pelo seu sistema imunológico. Foi também salientado que o destino da Leishmania no interior de macrófagos depende de relações particulares parasito-hospedeiro, envolvendo não apenas as propriedades intrísecas do parasita, mas também as características, geneticamente determinadas, da cédula hospedeira ou das suas interações com outras células imunocompetentes. Atráves de uma revisão das evidências consubstanciando essesconceitos, os mesmos foram aplicados na descrição do espectro clínico e imunopatológico da doença humana, particularmente das leishmanioses cutâneas e mucocutâneas do Novo e do Velho Mundo. Finalmente, baseando-se nos resultados obtidos em modelos experimentais de leishmaniose cutânea, os quais reproduzem os achados das formas resolutiva e persistentes da doença humana, o autor apresenta uma análise esquemática da evolução das características histopatológicas das lesões leishmanióticas.<br>Parasites of the genus Leishmania induce a complex variety of responses in their mammalian hosts, effected and/or modulated by the immune system. The outcome of leishmanial infection of macrophages depends on factors particular to each host-parasite combination, involving not only intrinsic properties of the parasite, but also genetically determined characteristics of the host cell or of its interactions with immunocompetent cells. Following a review of evidence supporting these concepts, they are applied in describing the clinical and immunopathological spectrum of the disease as it occurs in man, with emphasis on the cutaneous and mucocutaneous leishmaniases of the Old and New World. Based on results obtained in experimental models of cutaneous leishmaniasis which reproduce features of healing and non-healing human disease, the author presents a schematic analysis of the evolution of the histopathological features of leishmanial lesions

Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania

Submitted by Sandra Infurna ([email protected]) on 2019-11-28T14:01:19Z No. of bitstreams: 1 YaraMariaT_Cseko_etal_IOC_1992.pdf: 448633 bytes, checksum: 8c35e52a3754ed36d283a72b96a8601e (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2019-11-28T14:15:23Z (GMT) No. of bitstreams: 1 YaraMariaT_Cseko_etal_IOC_1992.pdf: 448633 bytes, checksum: 8c35e52a3754ed36d283a72b96a8601e (MD5)Made available in DSpace on 2019-11-28T14:15:23Z (GMT). No. of bitstreams: 1 YaraMariaT_Cseko_etal_IOC_1992.pdf: 448633 bytes, checksum: 8c35e52a3754ed36d283a72b96a8601e (MD5) Previous issue date: 1992Universidade do Estado do Rio de Janeiro. Instituto de Biologia. Departamento de Biologia Celular e Genética. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica Biologia Celular. Rio de Janeiro, RJ, Brasil.The molecular karyotypes for 20 reference strains of species complexes of Leishmania were determined by contour-clamped homogeneous electric field (CHEF) electrophoresis. Determination of number/position of chromosome-sized bands and chromosomal DNA locations of housekeeping genes were the two criteria used for differentiating and classifying the Leishmania species. We have established two gel running conditions for optimal separation of chromosomes, which resolved DNA molecules as large as 2,500 kilobase pairs (kb). Chromosomes were polymorphic in number (22-30) and size (200-2,500 kb) of bands among members of five complexes of Leishmania. Although each stock had a distinct karyotype, in general the differences found between strains and/or species within each complex were not clear enough for parasite identification. However, each group showed a specific number of size-concordant DNA molecules, which allowed distinction among the Leishmania complex parasites. Clear differences between the Old and New world groups of parasites or among some New World Leishmania species were also apparent in relation to the chromosome locations of beta-tubulin genes. Based on these results as well as data from other published studies the potential of using DNA karyotype for identifying and classifying leishmanial field isolates is discussed