Determining the relationship between individual polymorphic alleles and haplotypes of genes of cytokines IL-1β and its receptor antagonist (IL-1RN) to predict the nature of chronic periodontal inflammation

Found that carriers of the T allele of the polymorphic locus of С-511T of IL-1β and genotype С / T met significantly more likely in the group of patients with severe periodontitis In the study of nucleotide substitution of genes IL-1β С +3953 T allele T is also more common in the study group in patients with periodontitis In the study of gene polymorphism IL-1 RN significant differences between treatment groups were found. There is a tendency to increase the number of carriers of allele 2R, genotype 2R/4R and 2R/2R among persons with severe periodontal inflammation.Установлено, что носители Т аллеля полиморфного локуса С-511Т ИЛ-1β и генотипа СЯ встречались значимо чаще в группе пациентов, больных тяжелыми формами пародонтита. При исследовании нуклеотидной замены генов IL-1βС+3953Т аллель Т также чаще встречался в основной группе у больных пародонтитом. При исследовании полиморфизма генов IL-1RN достоверных различий между исследуемыми группами обнаружено не было. Имеется тенденция к увеличению количества носителей аллеля 2R, генотипа 2R/4R и 2R/2R среди лиц с тяжелым течением воспаления пародонта

Histological and immunohistochemical criteria of chronic inflammatory periodontal in remission

The article analyzes the changes in clinical indicators of periodontal patients on the background of the basic course of treatment. In gingival biopsies of patients estimated to update the cell population of multilayer flat epithelium with joint use of markers Ki-67 and p53, the degree of acanthosis and inflammatory cells own plate of mucous membrane on the number of CD68 + cells. 'Stationary' phase of inflammation is characterized by a decrease in inflammatory infiltration and is accompanied by a normalization process of cell renewal in the epithelium associated with the suppression of proliferation of epithelial cells and enhance their elimination through apoptosis. The number of antigen cells (CD68 +) in the mucosa of the gums was not significantly changed at the end of the course of traditional therapy and is the morphological substrate of recurrence of inflammation.В статье проводится анализ изменения клинических показателей состояния пародонта пациентов на фоне проведения базового курса лечения. В биоптатах десны пациентов оценивается обновление клеточной популяции многослойного плоского эпителия при совместном применении маркеров Ki-67 и р53,степени акантоза и клеток воспалительного инфильтрата собственной пластинки слизистой оболочки по количеству CD68+ клеток. «Стационарная» стадия воспаления характеризуется уменьшением воспалительной инфильтрации и сопровождается нормализацией процессов клеточного обновления в эпителии, связанного с подавлением пролиферации эпителиоцитов и усилением их элиминации путем апоптоза. Количество антигенпрезентирующих клеток (CD68+) в слизистой оболочке десны значимо не изменяется по окончании курса традиционной терапии и является морфологическим субстратом рецидива воспаления

The clinical course of chronic generalized periodontitis and polymorphisms of genes key inflammatory cytokines IL-1β, IL-1 RN, TNFα, IL-4.

On the basis of analysis of medical history, clinical, radiological (speed and nature of the resorption of supporting bone structures) survey data of 154 patients with chronic generalized periodontitis isolated group 'aggressive' ‘moderately progressive' and 'progressing slowly* over the disease. In analyzing the distribution of polymorphic alleles of genes of inflammatory cytokines -511T + 3953T gene IL-1β, 2R (VNTR in intron 2) gene IL-1 RN, gene -308A TNFα, 3R (VNTR in intron 3) IL-4 gene revealed the association ‘ aggressive flow with carriage alleles 2R (VNTR in intron 2) gene and the IL-1 RN gene -308A TNFα; 'Moderately progressive' course of joint carriage of -511 alleles T, + 3953T gene IL-1β.На основании анализа анамнестических, клинических, рентгенологических (скорость и характер резорбции опорных костных структур) данных обследования 154 больных хроническим генерализованным пародонтитом выделены группы с «агрессивным» «умеренно прогрессирующим» и «медленно прогрессирующим» течением болезни. При анализе распределения полиморфных аллелей генов воспалительных цитокинов -511Т, +3953Т гена IL-1β, 2R (VNTR во 2 интроне) гена IL-1RN, -308А гена TNFα, 3R (VNTR в 3 интроне) гена IL-4 выявлены ассоциации «агрессивного» течения с носительством аллелей 2R (VNTR во 2 интроне) гена IL-1RN и -308А гена TNFα; «умеренно прогрессирующего» течения с совместным носительством аллелей -511Т, +3953Т гена IL-1β

A protocol for co-creating research project lay summaries with stakeholders:Guideline development for Canada's AGE-WELL Network

Background Funding bodies increasingly require researchers to write lay summaries to communicate projects’ real-world relevance to the public in an accessible way. However, research proposals and findings are generally not easily readable or understandable by non-specialist readers. Many researchers find writing lay summaries difficult because they typically write for fellow subject specialists or academics rather than the general public or a non-specialist audience. The primary objective of our project is to develop guidelines for researchers in Canada’s AGE-WELL Network of Centres of Excellence, and ultimately various other disciplines, sectors, and institutions, to co-create lay summaries of research projects with stakeholders. To begin, we produced a protocol for co-creating a lay summary based on workshops we organized and facilitated for an AGE-WELL researcher. This paper presents the lay summary co-creation protocol that AGE-WELL researchers will be invited to use. Methods Eligible participants in this project will be 24 AgeTech project researchers who are funded by the AGE-WELL network in its Core Research Program 2020. If they agree to participate in this project, we will invite them to use our protocol to co-produce a lay summary of their respective projects with stakeholders. The protocol comprises six steps: Investigate principles of writing a good lay summary, identify the target readership, identify stakeholders to collaborate with, recruit the identified stakeholders to work on a lay summary, prepare for workshop sessions, and execute the sessions. To help participants through the process, we will provide them with a guide to developing an accessible, readable research lay summary, help them make decisions, and host, and facilitate if needed, their lay summary co-creation workshops. Discussion Public-facing research outputs, including lay summaries, are increasingly important knowledge translation strategies to promote the impact of research on real-world issues. To produce lay summaries that include information that will interest a non-specialist readership and that are written in accessible language, stakeholder engagement is key. Furthermore, both researchers and stakeholders benefit by participating in the co-creation process. We hope the protocol helps researchers collaborate with stakeholders effectively to co-produce lay summaries that meet the needs of both the public and project funders

Clinical value of definition of an expression of molecule CD68 + cells inflammatory infiltration in biopsy gums at patients with gingivitis and periodontitis at treatment stages

In article changes of a clinical condition of periodontium tissue, degrees of expressiveness inflammatory infiltration in biopsy gums of 53 patients with gingivitis and periodontitis are analyzed. Immunophentyping cells of inflammatory infiltration in biopsy gums of patients, has revealed system cells mononuclear phagocytes (CD68 +) in a slight stages inflammatory changes that is a morphological substratum of possible relapse of an inflammation in periodontium.В статье анализируются изменения клинического состояния тканей пародонта, степени выраженности воспалительного инфильтрата в биоптатах десны 53 больных гингивитом и пародонтитом. Иммунофенотипирование клеток воспалительного инфильтрата в биоптатах десны пациентов, выявило клетки системы мононуклеарных фагоцитов (CD68+) в стадию слабовыраженных воспалительных изменений, что является морфологическим субстратом возможного рецидива воспаления в пародонте

Predictive value of risk factors for periodontitis and assessment criteria of the flow of inflammation in periodontitis on the basis of molecular genetic tests

The article analyzes the predictive value of risk factors for periodontitis, the data obtained from a prospective observation of 150 patients. Evaluation of the flow of the process of inflammation was carried out according to morphological study. Saving inflammatory infiltrate in the background of the anti-microbial treatment stage, accompanied by marked clinical effect was regarded as 'poor' outcome of therapy. Discriminant analysis of anamnestic, clinical, morphological and molecular genetic characteristics of patients allowed to calculate graduation factors predicting. The greatest predictive value is a predictor of 'carrier 2R allele of the gene IL-1 RN, IL-1 family1. Following graduation in molecular genetics predictor is ‘3R allele of the gene IL-4 and the last one -'allele-308A' gene TNFα». Of the predictors of having a digital expression, the greatest predictive value are: the 'age' and 'Limitation of the disease’. This combination of predictors can provide the level of correct prediction in 79.8%.В статье проведен анализ прогнозной ценности факторов риска пародонтита, данные получены в результате проспективного наблюдения 150 больных. Оценка характера течения процесса воспаления проводилась по данным морфологического исследования. Сохранение воспалительного инфильтрата на фоне проведенного противомикробного этапа лечения, сопровождавшегося выраженным клиническим эффектом, расценивали как «неблагоприятный» исход терапии. Дискриминантный анализ анамнестических, клинических, морфологических и молекулярно-генетических характеристик пациентов позволил рассчитать градацию факторов прогнозирования. Наибольшую прогнозную ценность имеет предиктор «носительство аллеля 2R гена IL-1RN, семейства IL-1». Следующими по градации молекулярно-генетическим предиктором является «аллель 3R гена IL-4» и последним - «аллель -308А* гена TNFα». Из предикторов, имеющих цифровое выражение, наибольшую прогнозную ценность имеют: «возраст» и «давность болезни». Данное сочетание предикторов позволяет обеспечить уровень корректного прогноза в 79,8%

Distribution pattern of TLR-2, TLR-4, CD68, CD45R0, interacting with the antigens parodontopatogens in the cells of mucosal gingival in patients with chronic generalized periodontitis

The article analyzes the expression of TLR-2 and TLR-4 receptor of epithelial cells, the distribution of CD68 positive cells and CD45R0 positive T lymphocyte inflammatory infiltrate in the compartments of the gums of patients with chronic generalized periodontitis of medium severity on the background of the initial course of periodontal treatment. Treatment included pharmacological - machining of teeth, tooth roots, periodontal pockets, teaching oral hygiene using the ruler of 'ASEPTA', eliminating areas for retention of microbial plaque, temporary splinting and prosthetics. An expression is derived clinical benefit. Before treatment was determined by high expression of TLR-2 and TLR-4 on the surface of epithelial cells of the gums, and in the inflammatory infiltrate detected a large number of cells of the immune synapse of participants (CD45R0-T lymphocytes and CD68 + macrophages). After treatment significantly decreased expression of TLR-2 and TLR-4 on the surface of epithelial cells of the gums, while in his own plate of mucous membrane gums preserved population CD68 + macrophages, CD45R0 T lymphocytes, indicating the reduction of the barrier function of the epithelium and capable to run the aggravation of inflammation in the case of excessive growth parodontopatogenov in the area of periodontal pockets.В статье проведен анализ характера экспрессии TLR-2 и TLR-4 рецепторов зпителиоцитов, распределения CD68 позитивных клеток и CD45RO позитивных Т лимфоцитов воспалительного инфильтрата в компартментах десны больных хроническим генерализованным пародонтитом средней степени тяжести на фоне начального курса пародонтологического лечения. Лечение включало фармако - механическую обработку зубов, корней зубов, пародонтальных карманов, обучение гигиене полости рта с использованием линейки средств «АСЕПТА». устранения зон ретенции для микробного налета, временное шинирование и протезирование зубов. Получен выраженный клинический эффект. До лечения определялся высокий уровень экспрессии TLR-2 и TLR-4 на поверхности зпителиоцитов десны, а в воспалительном инфильтрате зафиксировано большое количество клеток участников иммунного синапса (С045Р0-Т-лимф0циты и CD68+ макрофаги). После лечения статистически значимо уменьшилась экспрессия TLR-2 и TLR-4 на поверхности эпителиоцитов десны, при этом в собственной пластинке слизистой оболочки десны сохранились популяции CD68+ макрофагов CD45RO Т лимфоцитов, свидетельствующие о снижение барьерной функции эпителия и способные запустить обострение воспаления в случае избыточного роста пародонтопатогенов в зоне пародонтальных карманов

Status of ART-XC/SRG Instrument

Spectrum Roentgen Gamma (SRG) is an X-ray astrophysical observatory, developed by Russia in collaboration with Germany. The mission will be launched in March 2016 from Baikonur, by a Zenit rocket with a Fregat booster and placed in a 6-month-period halo orbit around L2. The scientific payload consists of two independent telescopes - a soft-x-ray survey instrument, eROSITA, being provided by Germany and a medium-x-ray-energy survey instrument ART-XC being developed by Russia. ART-XC will consist of seven independent, but co-aligned, telescope modules. The NASA Marshall Space Flight Center (MSFC) is fabricating the flight mirror modules for the ART-XC/SRG. Each mirror module will be aligned with a focal plane CdTe double-sided strip detectors which will operate over the energy range of 6-30 keV, with an angular resolution of less than 1, a field of view of approximately 34 and an expected energy resolution of about 10 percent at 14 keV

The ART-XC telescope on board the SRG observatory

ART-XC (Astronomical Roentgen Telescope - X-ray Concentrator) is the hard X-ray instrument with grazing incidence imaging optics on board the Spektr-Roentgen-Gamma (SRG) observatory. The SRG observatory is the flagship astrophysical mission of the Russian Federal Space Program, which was successively launched into orbit around the second Lagrangian point (L2) of the Earth-Sun system with a Proton rocket from the Baikonur cosmodrome on 13 July 2019. The ART-XC telescope will provide the first ever true imaging all-sky survey performed with grazing incidence optics in the 4-30 keV energy band and will obtain the deepest and sharpest map of the sky in the energy range of 4-12 keV. Observations performed during the early calibration and performance verification phase as well as during the on-going all-sky survey that started on 12 Dec. 2019 have demonstrated that the in-flight characteristics of the ART-XC telescope are very close to expectations based on the results of ground calibrations. Upon completion of its 4-year all-sky survey, ART-XC is expected to detect ~5000 sources (~3000 active galactic nuclei, including heavily obscured ones, several hundred clusters of galaxies, ~1000 cataclysmic variables and other Galactic sources), and to provide a high-quality map of the Galactic background emission in the 4-12 keV energy band. ART-XC is also well suited for discovering transient X-ray sources. In this paper, we describe the telescope, results of its ground calibrations, major aspects of the mission, the in-flight performance of ART-XC and first scientific results.Comment: 19 pages, 30 figures, accepted for publication in Astronomy and Astrophysic