El periodismo ciudadano y la radio escolar como estrategia de fortalecimiento de las competencias comunicativas en los estudiantes de grado 9° del colegio Saulo de Tarso en Soacha

Fortalecer las competencias comunicativas y pensamiento crítico en los estudiantes de grado 9 de la I.E Saulo de Tarso en Soacha a través del periodismo ciudadano.El presente proyecto de investigación surge a partir de un proceso que se llevó a cabo en el Colegio Saulo de Tarso del municipio de Soacha, con estudiantes del grado noveno, en donde se implementó una alternativa de aprendizaje significativa con el propósito de fortalecer algunas debilidades de estudio, por medio de prácticas asociadas con el periodismo ciudadano, se planteó y ejecuto una propuesta de intervención denominada “El periodismo ciudadano y la radio escolar como estrategia de fortalecimiento del pensamiento crítico en los estudiantes de grado 9° del colegio Saulo de Tarso en Soacha”, enfocado en la Investigación Cualitativa, siguiendo un diseño metodológico apoyado con los instrumentos de investigación como son la observación, entrevista, encuesta y talleres, los cuales permitieron un resultado evidente frente a los logros obtenidos; de tal modo en dicho proceso se incentivaron habilidades narrativas, comprensión de lectura, búsqueda de información, contraste de fuentes, opiniones y por medio del uso de las Tic´s los estudiantes exploraron herramientas técnicas adquiriendo conocimientos en productos radiofónicos como son los podcast. Es así como de manera estratégica el proceso que dirige el periodismo ciudadano y la radio escolar influye en el fortalecimiento del pensamiento crítico.This research project arises from a process that was carried out at the Colegio Saulo de Tarso in the municipality of Soacha, with ninth grade students, where an alternative of meaningful learning was implemented with the purpose of strengthening some weaknesses of study, through practices associated with citizen journalism, an intervention proposal called "Citizen journalism and school radio as a strategy to strengthen critical thinking in 9th grade students of the Saulo de Tarso school in Soacha" was proposed and executed. ”, focused on qualitative research, following a methodological design supported by research instruments such as observation, interview, survey and workshops, which allowed an evident result compared to the achievements obtained; Thus, in this process, narrative skills, reading comprehension, information search, contrasting sources, opinions were encouraged, and through the use of ICTs, the students explored technical tools, acquiring knowledge in radio products such as podcasts. This is how, in a strategic way, the process that directs citizen journalism and school radio influences the strengthening of critical thinking

Antineoplastic activity of idazoxan hydrochloride

Idazoxan hydrochloride (IDA) is a 241 molecular weight imidazoline and adrenoreceptor ligand. It binds to mitochondrial membranes and promotes apoptosis of pancreatic beta cells. Since IDA has not been tested against tumor cells, the purpose of our study was to determine if IDA has antineoplastic activity. We used the conversion of a soluble tetrazolium salt to an insoluble formazan precipitate and differential staining cytotoxicity assays to determine if IDA was cytotoxic to cell lines of murine lung cancer and human prostate cancer, as well as to a variety of fresh human tumor samples. We used flow cytometry to analyze cell death and calreticulin expression. IDA is cytotoxic to both cell lines and against aliquots of specimens of breast, gastric, lung, ovarian and prostate cancers as well as non-Hodgkin’s lymphoma. It produces apoptotic cell death and promotes calreticulin expression, suggesting that IDA might be immunomodulatory in vivo. We anticipate that IDA will be clinically useful in cancer treatment

0260 Imidazoline I1 receptor ligands activate hepatic adiponectin pathways and thus improve insulin sensitivity

Metabolic syndrome is defined as a cluster of cardiovascular and metabolic disorders. Previous studies in rat models of metabolic syndrome have demonstrated that ligands selective for I1 imidazoline receptor (LNPs) increase insulin sensitivity through central sympathoinhibition and an additional peripheral effect attributable to adiponectin, a major insulin-sensitizer adipokine. The objective of this study was to explore possible direct actions on hepatocytes, one of the target cells of insulin and adiponectin.Experiments were carried out in HepG2 cells, a cell line of hepatocytes. In order to evaluate the effect of LNPs on insulin sensitivity, the activation (i.e. phosphorylation) of a key actor of insulin pathways, AKT, was evaluated by measuring the ratio pAKT/AKT by Western Blot. Similarly, the effect of LNPs on adiponectin signaling was evaluated by measuring the rate of phosphorylation of the central kinase involved in adiponectin pathways, AMPK, by Western Blot. Insulin (10μM) induced the phosphorylation of AKT (pAKT/ AKT=0.49±0.16) compared to control without insulin (pAKT/AKT=0.11±0.03; p≤0.05) whereas LNPs (1μM) alone did not. Interestingly, pretreatment by LNPs (1μM) during 60 min could potentiate the insulin-induced activation of AKT: LNP509: pAKT/AKT=1.13±0.18 (p≤0.05 vs insulin alone); LNP599: pAKT/AKT=1.23±0.16 (p=0.0545 vs insulin alone).Concerning adiponectin signaling pathways, LNPs alone (from 10−9M to 10−4M) increased AMPK phosphorylation in a concentration- and time-dependent manner. The maximal effect was obtained after 10 min exposure of LNPs 10μM (untreated cells: pAMPK/AMPK=0.18±0.04; LNP 509 pAMPK/ AMPK=0.38±0.05 p≤0.05; LNP599 pAMPK/AMPK=0.46±0.17). These data suggest that LNPs on hepatic cells activate adiponectin pathways and potentiate insulin action. These two direct effects on insulin sensitive cells could account for the ameliorated insulin sensitivity observed in vivo