Guide for third and fourth year students

Advice complied by Boston University School of Medicine students for incoming first year students and third or fourth year students preparing for clinical rotations

A Data Driven Analysis of Rural Equity and Cost Concerns for Mileage-Based User Fees in Vermont

Examining substitutes to the current state and federal gasoline and diesel fuel excise taxes has become a pressing issue, exacerbated by the rise of high efficiency and alternative fuel vehicles threatening the revenue generating capacity of these taxes. A mileage-based user fee has been frequently proposed in the literature as an alternative which would offer greater benefits to rural and low-income populations than to urban and higher income populations. However, most prior analyses relied on small data sets and aggregated data. This study examined the impact of replacing the Vermont state fuels tax with a revenue-neutral mileage-based user fee using mileage and fuel economy data for over 300,000 registered passenger vehicles. We find that, on average, Vermont households would pay an additional $23 per year, with rural households and low-income households facing smaller tax burdens than their urban and high-income counterparts. The impacts of a$180 flat fee replacing the Vermont state motor fuels tax was also examined due to state interest. Findings indicate a flat fee would result in much larger price fluctuations, with most households paying an additional $47 per year. The disaggregated data approach presented here directly addresses public misconceptions of inequitable cost differences and provides context for public education campaigns to garner mileage-based user fee policy support. Based on our results, there is political ground for further research into the implementation of a mileage-based user fee, including the logistics of an administrative transition to mileage charging and the associated program implementation and technological costs

The Role of Information and Education on Modifying Support for Gas Tax Alternatives using an Informed Choice Questionnaire

In the United States, transportation fuel excise taxes commonly referred to as the “gas tax” provide a large share of funding to state agencies for transportation maintenance and construction projects. The revenue generating capacity of the gas tax is threatened by increasing vehicle fuel efficiency, electric vehicles adoption and inflation. Alternative funding schemes are necessary, but public acceptance of tax changes is low. We surveyed residents of Vermont, Maine, and New Hampshire to gauge understanding of and support for a mileage-based user fee and a flat fee as potential replacements for the current gas tax and the potential to modify support through education. Throughout the survey, respondents were provided information and learning opportunities to “myth bust” common misconceptions about the gas tax and the potential policy alternatives. We find that, before education, respondents knew very little about how the current gas tax works and showed minimal support for the proposed policy alternatives. Post education, support for mileage-based user fees increased by 11%. Using regression modelling, we determined that respondent attitudes and beliefs, travel behavior, community type, and personalized cost estimates were all highly significant predictors of policy support. Overall, we find responding to common public concerns with up-to-date and non-biased information within a relatively simple learning experience can cause substantial changes in policy support. Our findings offer an avenue to understand how support for gas tax alternatives varies amongst different groups of people and the role that education can play in increasing policy support in the face of widespread misconceptions

The Impact on Informal Supporters of Domestic Violence Survivors:A Systematic Literature Review

Domestic violence (DV) is experienced by 1 in 4 women in the United Kingdom during their lifetime, and most survivors will seek informal support from the people around them, even if they choose not to access help from professionals. Support from these relatives, friends, neighbors, and colleagues can provide a buffer against effects on the survivor’s physical health, mental health, and quality of life, and has been shown to be protective against future abuse. There has been an absence of research studying members of survivors’ networks and, in particular, investigating how the impact of DV might diffuse to affect them. A systematic literature review of reported research (either in peer-reviewed journals or in gray literature) was undertaken to explore the impacts of DV on survivor networks. Of the articles found, 24 had data relating to the topic area, though no study addressed the question directly. Framework analysis and meta-ethnography generated the following themes: physical health impacts, negative impacts on psychological well-being, direct impacts from the perpetrator, and beneficial impacts on psychological well-being. The studies in this review indicated that informal supporters may be experiencing substantial impact, including vicarious trauma and the risk of physical harm. Currently, there is little support available which is directly aimed at informal supporters of DV survivors, thus these findings have practical and policy implications, in order to acknowledge and meet their needs. </jats:p

Home to School and School to Home: Syncretised Literacies in Linguistic Minority Communities

This paper argues for the need to move beyond the paradigm of parental involvement in children's early literacy through story-reading practice, which presently informs home/school reading programmes, to consider a wider framework for family and community involvement. The first part of the paper examines the literature informing the current model showing the marked absence of studies on the different literacy practices in which children from new immigrant/minority ethnic families engage and which may be different from those of their teachers. This prevailing mainstream paradigm is illustrated through the experiences of two young children reading with their mother and sister. The second part of the paper draws upon findings from research projects investigating the home, school and community reading practices of new immigrant families in east London and compares them with families that do conform to the prevailing paradigm for successful involvement. Finally, it suggests principles for inclusive education for minority families

Comparing Travel Behavior and Opportunities to Increase Transportation Sustainability in Small Cities, Towns and Rural Communities

The vast majority of travel behavior and sustainable transportation research has focused on urban areas. A rural perspective is lacking. This paper aims to dive deeper into understanding how people travel and their perceptions and opinions about various components of travel in a majority rural state. By speaking directly with Vermonters through in-person interviews, uniquely personal points of view were discovered and analyzed to find commonalities and differences between urban, suburban, and rural Vermonters. Questions on day-to-day challenges of traveling, suggestions for reducing greenhouse gas emissions, responses to fuel prices, and opinions on electric vehicles were asked. Some key findings were illuminated over the interview and analysis processes. Rural areas struggle most with traveling long distances to reach services, while urban areas are more concerned with traffic, opinions on EV ownership were consistent across the state, with people being likely to consider owning an EV if costs of ownership were to decrease. These interviews highlighted additional questions that need to be answered so that states can develop practical and effective policies aimed at reducing greenhouse gas emissions in rural areas. Further in-depth surveys are recommended coming out of this study so that a more complete picture of potential behavioral shifts can be attained. A reduction in GHG emissions is paramount to the world’s survival and this research will add to the body of knowledge in a historically understudied population so that we can work more closely with small and rural communities to help combat climate change through widespread emission reduction

Diversity, differentiation, and linkage disequilibrium: prospects for association mapping in the malaria vector anopheles arabiensis

Association mapping is a widely applied method for elucidating the genetic basis of phenotypic traits. However, factors such as linkage disequilibrium and levels of genetic diversity influence the power and resolution of this approach. Moreover, the presence of population subdivision among samples can result in spurious associations if not accounted for. As such, it is useful to have a detailed understanding of these factors before conducting association mapping experiments. Here we conducted whole-genome sequencing on 24 specimens of the malaria mosquito vector, Anopheles arabiensis, to further understanding of patterns of genetic diversity, population subdivision and linkage disequilibrium in this species. We found high levels of genetic diversity within the An. arabiensis genome, with ~800,000 high-confidence, single- nucleotide polymorphisms detected. However, levels of nucleotide diversity varied significantly both within and between chromosomes. We observed lower diversity on the X chromosome, within some inversions, and near centromeres. Population structure was absent at the local scale (Kilombero Valley, Tanzania) but detected between distant populations (Cameroon vs. Tanzania) where differentiation was largely restricted to certain autosomal chromosomal inversions such as 2Rb. Overall, linkage disequilibrium within An. arabiensis decayed very rapidly (within 200 bp) across all chromosomes. However, elevated linkage disequilibrium was observed within some inversions, suggesting that recombination is reduced in those regions. The overall low levels of linkage disequilibrium suggests that association studies in this taxon will be very challenging for all but variants of large effect, and will require large sample sizes

Changes in the gut microbiota of mice orally exposed to methylimidazolium ionic liquids

Ionic liquids are salts used in a variety of industrial processes, and being relatively non-volatile, are proposed as environmentally-friendly replacements for existing volatile liquids. Methylimidazolium ionic liquids resist complete degradation in the environment, likely because the imidazolium moiety does not exist naturally in biological systems. However, there is limited data available regarding their mammalian effects in vivo. This study aimed to examine the effects of exposing mice separately to 2 different methylimidazolium ionic liquids (BMI and M8OI) through their addition to drinking water. Potential effects on key target organs-the liver and kidney-were examined, as well as the gut microbiome. Adult male mice were exposed to drinking water containing ionic liquids at a concentration of 440 mg/L for 18 weeks prior to examination of tissues, serum, urine and the gut microbiome. Histopathology was performed on tissues and clinical chemistry on serum for biomarkers of hepatic and renal injury. Bacterial DNA was isolated from the gut contents and subjected to targeted 16S rRNA sequencing. Mild hepatic and renal effects were limited to glycogen depletion and mild degenerative changes respectively. No hepatic or renal adverse effects were observed. In contrast, ionic liquid exposure altered gut microbial composition but not overall alpha diversity. Proportional abundance of Lachnospiraceae, Clostridia and Coriobacteriaceae spp. were significantly greater in ionic liquid-exposed mice, as were predicted KEGG functional pathways associated with xenobiotic and amino acid metabolism. Exposure to ionic liquids via drinking water therefore resulted in marked changes in the gut microbiome in mice prior to any overt pathological effects in target organs. Ionic liquids may be an emerging risk to health through their potential effects on the gut microbiome, which is implicated in the causes and/or severity of an array of chronic disease in humans

Design and applicability of DNA arrays and DNA barcodes in biodiversity monitoring

<p>Abstract</p> <p>Background</p> <p>The rapid and accurate identification of species is a critical component of large-scale biodiversity monitoring programs. DNA arrays (micro and macro) and DNA barcodes are two molecular approaches that have recently garnered much attention. Here, we compare these two platforms for identification of an important group, the mammals.</p> <p>Results</p> <p>Our analyses, based on the two commonly used mitochondrial genes cytochrome <it>c </it>oxidase I (the standard DNA barcode for animal species) and cytochrome b (a common species-level marker), suggest that both arrays and barcodes are capable of discriminating mammalian species with high accuracy. We used three different datasets of mammalian species, comprising different sampling strategies. For DNA arrays we designed three probes for each species to address intraspecific variation. As for DNA barcoding, our analyses show that both cytochrome <it>c </it>oxidase I and cytochrome b genes, and even smaller fragments of them (mini-barcodes) can successfully discriminate species in a wide variety of specimens.</p> <p>Conclusion</p> <p>This study showed that DNA arrays and DNA barcodes are valuable molecular methods for biodiversity monitoring programs. Both approaches were capable of discriminating among mammalian species in our test assemblages. However, because designing DNA arrays require advance knowledge of target sequences, the use of this approach could be limited in large scale monitoring programs where unknown haplotypes might be encountered. DNA barcodes, by contrast, are sequencing-based and therefore could provide more flexibility in large-scale studies.</p

Reward, Context, and Human Behaviour

Animal models of reward processing have revealed an extensive network of brain areas that process different aspects of reward, from expectation and prediction to calculation of relative value. These results have been confirmed and extended in human neuroimaging to encompass secondary rewards more unique to humans, such as money. The majority of the extant literature covers the brain areas associated with rewards whilst neglecting analysis of the actual behaviours that these rewards generate. This review strives to redress this imbalance by illustrating the importance of looking at the behavioural outcome of rewards and the context in which they are produced. Following a brief review of the literature of reward-related activity in the brain, we examine the effect of reward context on actions. These studies reveal how the presence of reward vs. reward and punishment, or being conscious vs. unconscious of reward-related actions, differentially influence behaviour. The latter finding is of particular importance given the extent to which animal models are used in understanding the reward systems of the human mind. It is clear that further studies are needed to learn about the human reaction to reward in its entirety, including any distinctions between conscious and unconscious behaviours. We propose that studies of reward entail a measure of the animal's (human or nonhuman) knowledge of the reward and knowledge of its own behavioural outcome to achieve that reward