Case Report: Coincidental Inclusion in a 17-Locus Y-STR Mixture, Wrongful Conviction and Exoneration

We report the case of a suspect (Suspect-3) who was convicted (and later exonerated) of participating in the multiple-attacker rape of two women. The forensic evidence against him was his inclusion in a 17-marker Y-STR mixture isolated from semen on one victim’s clothing. The DNA inclusion produced a match statistic with a combined probability of inclusion of 1 in 741, and a Likelihood Ratio of 3296. While the defense team was told that Suspect-3 was included in the semen DNA mixture, they were not told that all of the Y-STR alleles could also be explained by just the other two accused attackers’ haplotypes. Suspect-3 was subsequently freed after the Taiwan Association for Innocence requested re-examination of the incriminating mixed DNA sample. The Criminal Investigation Bureau was then able to exclude him using an extended set of Y-STR markers (23 loci), leading to his exoneration

Biomarkers to identify and isolate senescent cells.

Aging is the main risk factor for many degenerative diseases and declining health. Senescent cells are part of the underlying mechanism for time-dependent tissue dysfunction. These cells can negatively affect neighbouring cells through an altered secretory phenotype: the senescence-associated secretory phenotype (SASP). The SASP induces senescence in healthy cells, promotes tumour formation and progression, and contributes to other age-related diseases such as atherosclerosis, immune-senescence and neurodegeneration. Removal of senescent cells was recently demonstrated to delay age-related degeneration and extend lifespan. To better understand cell aging and to reap the benefits of senescent cell removal, it is necessary to have a reliable biomarker to identify these cells. Following an introduction to cellular senescence, we discuss several classes of biomarkers in the context of their utility in identifying and/or removing senescent cells from tissues. Although senescence can be induced by a variety of stimuli, senescent cells share some characteristics that enable their identification both in vitro and in vivo. Nevertheless, it may prove difficult to identify a single biomarker capable of distinguishing senescence in all cell types. Therefore, this will not be a comprehensive review of all senescence biomarkers but rather an outlook on technologies and markers that are most suitable to identify and isolate senescent cells

Carbon Nanotube-Based Nonvolatile Random Access Memory for Molecular Computing

A concept for molecular electronics exploiting carbon nanotubes as both molecular device elements and molecular wires for reading and writing information was developed. Each device element is based on a suspended, crossed nanotube geometry that leads to bistable, electrostatically switchable ON/OFF states. The device elements are naturally addressable in large arrays by the carbon nanotube molecular wires making up the devices. These reversible, bistable device elements could be used to construct nonvolatile random access memory and logic function tables at an integration level approaching 1012 elements per square centimeter and an element operation frequency in excess of 100 gigahertz. The viability of this concept is demonstrated by detailed calculations and by the experimental realization of a reversible, bistable nanotube-based bit

Microstructural and geochemical constraints on the evolution of deep arc lithosphere

Mantle xenoliths from the Sierra Nevada, California, USA, sampled a vertical column (60–120 km) of lithosphere that formed during Mesozoic continental arc magmatism. This lithosphere experienced an anticlockwise P-T-t path resulting in rapid cooling that effectively “quenched in” features inherited from earlier high-temperature conditions. Here we combine new mineral chemistry data (water, trace element, and major element concentrations) with mineral crystallographic preferred orientations (CPOs) to investigate the relationship between melt infiltration and deformation. The peridotites record a refertilization trend with increasing depth, starting from shallow, coarse-protogranular, less-melt-infiltrated spinel peridotite with strong, orthorhombic olivine CPO to deep, fine-porphyroclastic, highly melt-infiltrated garnet peridotite with weak, axial-[010] olivine CPO. In contrast to the observed axial-[010] CPOs, subgrain boundary orientations and misorientation axes suggest the dominant activation of the (001)[100] slip system, suggesting deformation under moderately hydrous conditions. After accounting for effects of subsolidus cooling, we see coherent trends between mineral trace element abundance and water content, indicating that melt infiltration led to an increase in water content of the peridotites. However, measured olivine and pyroxene water contents in all peridotites (5–10 and 30–500 wt ppm, respectively) are lower than that required to promote dominant (001)[100] slip system observed in both natural and experimental samples. These results suggest that deformation occurred earlier along the P-T path, probably during or shortly after hydrous melt infiltration. Subsequent rapid cooling at 90 Ma led to water loss from olivine (owing to decreased solubility at low temperature), leaving behind a deep arc lithosphere that remained viscously coupled to the Farallon slab until the opening of the slab window in the late Cenozoic

Reconstruction of a meteotsunami in Lake Erie on 27 May 2012: Roles of atmospheric conditions on hydrodynamic response in enclosed basins

On 27 May 2012, atmospheric conditions gave rise to two convective systems that generated a series of waves in the meteotsunami band on Lake Erie. The resulting waves swept three swimmers a 0.5 mi offshore, inundated a marina, and may have led to a capsized boat along the southern shoreline. Analysis of radial velocities from a nearby radar tower in combination with coastal meteorological observation indicates that the convective systems produced a series of outflow bands that were the likely atmospheric cause of the meteotsunami. In order to explain the processes that led to meteotsunami generation, we model the hydrodynamic response to three meteorological forcing scenarios: (i) the reconstructed atmospheric disturbance from radar analysis, (ii) simulated conditions from a high‐resolution weather model, and (iii) interpolated meteorological conditions from the NOAA Great Lakes Coastal Forecasting System. The results reveal that the convective systems generated a series of waves incident to the southern shore of the lake that reflected toward the northern shoreline and reflected again to the southern shore, resulting in spatial wave focusing and edge wave formation that combined to impact recreational users near Cleveland, OH. This study illustrates the effects of meteotsunami development in an enclosed basin, including wave reflection, focusing, and edge wave formation as well as temporal lags between the causative atmospheric conditions and arrival of dangerous wave conditions. As a result, the ability to detect these extreme storms and predict the hydrodynamic response is crucial to reducing risk and building resilient coastal communities.Key Points:Radar‐based velocity allows for detection of meteotsunami‐inducing frontWave reflection, focusing, and edge waves led to meteotsunami conditionsEnclosed basins can induce temporal lags between storm front and wave responsePeer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/145384/1/jgrc21485_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/145384/2/jgrc21485-sup-0001-2015JC010883-s01.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/145384/3/jgrc21485.pd

Atmospheric Teleconnection over Eurasia Induced by Aerosol Radiative Forcing During Boreal Spring

The direct effects of aerosols on global and regional climate during boreal spring are investigated based on simulations using the NASA Global Modeling and Assimilation Office (GMAO) finite-volume general circulation model (fvGCM) with Microphyics of clouds in Relaxed Arakawa Schubert Scheme (McRAS). The aerosol loading are prescribed from three-dimensional monthly distribution of tropospheric aerosols viz., sulfate, black carbon, organic carbon, soil dust, and sea salt from output of the Goddard Ozone Chemistry Aerosol Radiation and Transport model (GOCART). The aerosol extinction coefficient, single scattering albedo, and asymmetric factor are computed as wavelength-dependent radiative forcing in the radiative transfer scheme of the fvGCM, and as a function of the aerosol loading and ambient relative humidity. We find that anomalous atmospheric heat sources induced by absorbing aerosols (dust and black carbon) excites a planetary scale teleconnection pattern in sea level pressure, temperature and geopotential height spanning North Africa through Eurasia to the North Pacific. Surface cooling due to direct effects of aerosols is found in the vicinity and downstream of the aerosol source regions, i.e., South Asia, East Asia, and northern and western Africa. Additionally, atmospheric heating is found in regions with large loading of dust (over Northern Africa, and Middle East), and black carbon (over South-East Asia). Paradoxically, the most pronounced feature in aerosol-induced surface temperature is an east-west dipole anomaly with strong cooling over the Caspian Sea, and warming over central and northeastern Asia, where aerosol concentration are low. Analyses of circulation anomalies show that the dipole anomaly is a part of an atmospheric teleconnection driven by atmospheric heating anomalies induced by absorbing aerosols in the source regions, but the influence was conveyed globally through barotropic energy dispersion and sustained by feedback processes associated with the regional circulations