Cytolytic T Lymphocytes Specific for Tumors and Infected Cells from Mice with a Retrovirus-induced Immunodeficiency Syndrome.

LP-BM5 retrovirus complex-infected C57BL/6 mice develop immunodeficiency, somewhat analogous to AIDS, termed murine AIDS (MAIDS). After secondary stimulation with syngeneic B-cell lymphomas from LP-BM5-infected mice, C57BL/6 mice produced vigorous CD8+ cytotoxic T lymphocytes specific for MAIDS-associated tumors. An anti-LP-BM5 specificity was suggested because spleen and lymph node cells from LP-BM5-infected mice served as target cells in competition assays, and cells from LP-BM5, but not ecotropic, virus-infected mice functioned as secondary in vitro stimulators to generate cytotoxic T lymphocytes to MAIDS tumors

A prosodic perspective on the assignment of tonal melodies to Arabic loanwords in Bambara

There is a rich descriptive history on Bambara tonology in the published literature (e.g. Bird 1966; Courtenay 1974; Creissels 1978, 1988, 1992; Diarra 1976; Dumestre 1987; Dwyer 1976). Despite the existence of several seminal works on the subject, certain details of the language’s tonal system remain unclear. Scholars have developed deep knowledge about the lexical and grammatical functions of Bambara tone, yet the dependency of tones and tonal processes on prosodic structure has only more recently been explored in detail (Green 2013, 2015; Leben 2002, 2003; Weidman and Rose 2006; Vydrine 2002, 2010). In this paper, we aim to contribute to this ongoing trend by considering a role played by prosodic structure in one particular set of Arabic borrowings for which the assignment of tonal melodies differs from that found in words of non-Arabic origin. We explore possible explanations for this divergence that relate to contemporary scholarship on the properties of Bambara’s prosodic structure. Our point of view on this subject differs from earlier analyses (e.g., Dumestre 1987) in that we propose that prosodic structure plays an important role in the assignment of Bambara tonal melodies. Finally, we relate our findings to a taxonomic model of loanword prosody in Davis et al. (2012) and consider the bearing that our findings may have on the typology of Bambara prosodic structure alongside other Mande languages.Il existe une riche histoire descriptive sur la tonologie du bambara dans la littérature publiée (par exemple Bird 1966, Courtenay 1974, Creissels 1978, 1988, 1992, Diarra 1976, Dumestre 1987, Dwyer 1976). Malgré l'existence de plusieurs travaux séminaires sur ce sujet, certains détails du système tonal de la langue demeurent obscurs. Les chercheurs ont développé une connaissance approfondie des fonctions lexicales et grammaticales du ton en bambara, mais la dépendance des tonalités et des processus tonals sur la structure prosodique n'a été étudiée que plus récemment (Green 2013, 2015, Weiden et Rose, 2006; Vydrine 2002, 2010). Dans cet article, nous cherchons à contribuer à cette tendance en considérant le rôle joué par la structure prosodique dans un ensemble particulier d'emprunts arabes pour lesquels l'attribution de mélodies tonales diffère de celle trouvée dans des mots d'origine non arabe. Nous explorons les explications possibles de cette divergence qui se rapportent à l'érudition contemporaine sur les propriétés de la structure prosodique du bambara. Notre point de vue sur ce sujet diffère des analyses antérieures (par exemple, Dumestre 1987) en ce que nous proposons que la structure prosodique joue un rôle important dans l'attribution de mélodies tonales en bambara. Enfin, nous relions nos découvertes à un modèle taxonomique de prosodie d’emprunts dans Davis et al. (2012) et considérons la portée que nos résultats peuvent avoir sur la typologie de la structure prosodique du bambara aux côtés d’autres langues mandées.Описанию тонологии бамана посвящена богатая литература (в частности, Bird 1966; Courtenay 1974; Creissels 1978, 1988, 1992; Diarra 1976; Dumestre 1987; Dwyer 1976). Имеющиеся публикации освещают ключевые вопросы в этой области, однако некоторые детали баманской тонологии всё же остаются неясными. Исследователи уделили много внимания лексическим и грамматическим функциям баманского тона, однако зависимость тонов и тональных процессов от просодической структуры была рассмотрена в деталях лишь недавно (Green 2013, 2015; Leben 2002, 2003; Weidman and Rose 2006; Vydrine 2002, 2010). Эта статья имеет целью укрепить это направление, проанализировав роль просодической структуры на конкретной выборке, а именно, на арабских заимствованиях, приписывание которым тоновых контуров подчиняется особым правилам. Рассматриваются различные объяснения таких различий, связанных с современными взглядами на особенности просодической структуры бамана. Наша точка зрения по этому вопросу отличается от позиций предшественников (см., в частности, Dumestre 1987), поскольку мы считаем, что в языке бамана просодическая структура играет важную роль в приписывании тональных контуров. Наконец, мы связываем наши результаты с таксономической моделью просодии заимствований в (Davis et al. 2012) и высказываем предположения о последствиях, которые эти результаты могут иметь для тонологии баманской просодической структуры, а также таковой в других языках манде

Ovine hepatogenous photosensitivity caused by the plant Nidorella foetida (Thunb.) DC. (Asteraceae)

Following a field outbreak of hepatogenous photosensitivity in sheep, an identical condition was reproduced experimentally by dosing 2 sheep with green, homogenized, Nidorella foetida material, collected from the camp where the outbreak occurred. This is a rare, unpalatable, aromatic shrub found in swampy places in the south-western Cape Province. The main lesion was a hepatosis, characterized by peripheral coagulative necrosis and midzonal degeneration in the 2 acute cases and mild bile duct proliferation and hepatic regeneration in the more chronic field case. Botanical, toxicological, clinical and pathological data are given.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.lmchunu2014mn201

Using wastewater-based epidemiology to estimate drug consumption—Statistical analyses and data presentation

Aim Analysis of wastewater samples can be used to assess population drug use, but reporting and statistical issues have limited the utility of the approach for epidemiology due to analytical results that are below the limit of quantification or detection. Unobserved or non-quantifiable—censored—data are common and likely to persist as the methodology is applied to more municipalities and a broader array of substances. We demonstrate the use of censored data techniques and account for measurement errors to explore distributions and annual estimates of the daily mean level of drugs excreted per capita. Measurements Daily 24-hour composite wastewater samples for 56 days in 2009 were obtained using a random sample stratified by day of week and season for 19 municipalities in the Northwest region of the U.S. Methods Methamphetamine, benzoylecgonine (cocaine metabolite), 3,4-ethylenedioxymethamphetamine (MDMA), methadone, oxycodone and hydrocodone were identified and quantified in wastewater samples. Four statistical approaches (reporting censoring, maximum likelihood estimation, Kaplan-Meier estimates, or complete data calculations) were used to estimate an annual average, including confidence bounds where appropriate, dependent upon the amount of censoring in the data. Findings The proportion of days within a year with censored data varied greatly by drug across the 19 municipalities, with MDMA varying the most (4% to 94% of observations censored). The different statistical approaches each needed to be used given the levels of censoring of measured drug concentrations. Figures incorporating confidence bounds allow visualization of the data that facilitates appropriate comparisons across municipalities. Conclusions Results from wastewater sampling that are below detection or quantification limits contain important information and can be incorporated to create a more complete and valid estimate of drug excretion

The Role of School Counselors Delivering a Trauma-Informed Care Approach to Supporting Youth in Foster Care

Through the development and implementation of a trauma-sensitive comprehensive school counseling program, school counselors can be essential in meeting the mental health needs of youth in foster care. The multitiered systems of support (MTSS) framework is one potential strategy in achieving this goal. We offer recommendations on how school counselors might deliver a trauma-informed care approach utilizing the MTSS framework to support the mental health needs of youth in foster care who have experienced trauma

Intrinsic Absorption in the Spectrum of NGC 7469: Simultaneous Chandra, FUSE, and STIS Observations

We present simultaneous X-ray, far-ultraviolet, and near-ultraviolet spectra of the Seyfert 1 galaxy NGC 7469 obtained with the Chandra X-Ray Observatory, the Far Ultraviolet Spectroscopic Explorer, and the Space Telescope Imaging Spectrograph on the Hubble Space Telescope. Previous non-simultaneous observations of this galaxy found two distinct UV absorption components, at -560 and -1900 km/s, with the former as the likely counterpart of the X-ray absorber. We confirm these two absorption components in our new UV observations, in which we detect prominent O VI, Ly alpha, N V, and C IV absorption. In our Chandra spectrum we detect O VIII emission, but no significant O VIII or O VII absorption. We also detect a prominent Fe K alpha emission line in the Chandra spectrum, as well as absorption due to hydrogen-like and helium-like neon, magnesium, and silicon at velocities consistent with the -560 km/s UV absorber. The FUSE and STIS data reveal that the H I and C IV column densities in this UV- and X-ray- absorbing component have increased over time, as the UV continuum flux decreased. We use measured H I, N V, C IV, and O VI column densities to model the photoionization state of both absorbers self-consistently. We confirm the general physical picture of the outflow in which the low velocity component is a highly ionized, high density absorber with a total column density of 10^20 cm^-2, located near the broad emission line region, although due to measurable columns of N V and C IV, we assign it a somewhat smaller ionization parameter than found previously, U~1. The high velocity UV component is of lower density, log N=18.6, and likely resides farther from the central engine as we find its ionization parameter to be U=0.08.Comment: Minor correction to abstract; STScI eprint #1683; 50 pages, incl. 19 figures, 4 tables; Accepted to Ap

Computational Studies on the Platinum(II)-Catalyzed Cycloisomerization of 1,6-Dienes into Bicyclopropanes: A Mechanistic Quandary Evaluated by DFT

The mechanism of the (bis(phosphanylethyl)phosphane)Pt2+ catalyzed cyclo-isomerization reaction of 7-methyl-octa-1,6-diene to form 1-isopropylbicyclo[3.1.0]hexane was studied using computational methods. The cyclopropanation step was found to be the turnover-limiting step. The overall reaction proceeds via both a 5-exo and a 6-endo route. W conformations were shown to facilitate cyclopropanation, but do not have any influence on the rate of the 1,2-hydride shifts

Intrinsic Absorption in the Spectrum of Mrk 279: Simultaneous Chandra, FUSE, and STIS Observations

We present a study of the intrinsic X-ray and far-ultraviolet absorption in the Seyfert 1.5 galaxy Markarian 279 using simultaneous observations from the Chandra X-ray Observatory, the Space Telescope Imaging Spectrograph aboard the Hubble Space Telescope, and the Far Ultraviolet Spectroscopic Explorer (FUSE). We also present FUSE observations made at three additional epochs. We detect the Fe K-alpha emission line in the Chandra spectrum, and its flux is consistent with the low X-ray continuum flux level of Mrk 279 at the time of the observation. Due to low signal-to-noise ratios in the Chandra spectrum, no O VII or O VIII absorption features are observable in the Chandra data, but the UV spectra reveal strong and complex absorption from HI and high-ionization species such as O VI, N V, and C IV, as well as from low-ionization species such as C III, N III, C II, and N II in some velocity components. The far-UV spectral coverage of the FUSE data provides information on high-order Lyman series absorption, which we use to calculate the optical depths and line and continuum covering fractions in the intrinsic HI absorbing gas in a self-consistent fashion. The UV continuum flux of Mrk 279 decreases by a factor of ~7.5 over the time spanning these observations and we discuss the implications of the response of the absorption features to this change. From arguments based on the velocities, profile shapes, covering fractions and variability of the UV absorption, we conclude that some of the absorption components, particularly those showing prominent low-ionization lines, are likely associated with the host galaxy of Mrk 279, and possibly with its interaction with a close companion galaxy, while the remainder arises in a nuclear outflow.Comment: To appear in 2004 May ApJS; double-column format; 58 pages, incl. 29 figures, 9 tables; minor changes to tex

Reactivity of a dititanium bis(pentalene) complex toward heteroallenes and main-group element–element bonds

The reactivity of the Ti═Ti double bond in (μ,η5:η5-Pn†)2Ti2 (1; Pn† = 1,4-{SiiPr3}2C8H4) toward isocyanide and heteroallene substrates, and molecules featuring homonuclear bonds between main-group elements (E–E) has been explored. Reaction of 1 with methyl isocyanide or 1,3-N,N′-di-p-tolylcarbodiimide resulted in the formation of the 1:1 adducts (μ,η5:η5-Pn†)2Ti2(μ,η2-CNMe) (2) and (μ,η5:η5-Pn†)2Ti2(μ-C{N(4-C6H4CH3)}2) (3), respectively, which are thermally stable up to 100 °C in contrast to the analogous adducts formed with CO and CO2. Reaction of 1 with phenyl isocyanate afforded a paramagnetic complex, [(η8-Pn†)Ti]2(μ,κ2:κ2-O2CNPh) (4), in which the “double-sandwich” architecture of 1 has been broken and an unusual phenyl-carbonimidate ligand bridges two formally Ti(III) centers. Reaction of 1 with diphenyl dichalcogenides, Ph2E2 (E = S, Se, Te), led to the series of Ti–Ti single-bonded complexes (μ,η5:η5-Pn†)2[Ti(EPh)]2 (E = S (5), Se (6), Te (7)), which can be considered the result of a 2e– redox reaction or a 1,2-addition across the Ti═Ti bond. Treatment of 1 with azobenzene or phenyl azide afforded [(η8-Pn†)Ti]2(μ-NPh)2 (8), a bridging imido complex in which the pentalene ligands bind in an η8 fashion to each formally Ti(IV) center, as the result of a 4e– redox reaction driven by the oxidative cleavage of the Ti═Ti double bond. The new complexes 2–8 were extensively characterized by various techniques including multinuclear NMR spectroscopy and single-crystal X-ray diffraction, and the experimental work was complemented by density functional theory (DFT) studies

Individualized decision aid for diverse women with lupus nephritis (IDEA-WON): A randomized controlled trial.

BackgroundTreatment decision-making regarding immunosuppressive therapy is challenging for individuals with lupus. We assessed the effectiveness of a decision aid for immunosuppressive therapy in lupus nephritis.Methods and findingsIn a United States multicenter, open-label, randomized controlled trial (RCT), adult women with lupus nephritis, mostly from racial/ethnic minority backgrounds with low socioeconomic status (SES), seen in in- or outpatient settings, were randomized to an individualized, culturally tailored, computerized decision aid versus American College of Rheumatology (ACR) lupus pamphlet (1:1 ratio), using computer-generated randomization. We hypothesized that the co-primary outcomes of decisional conflict and informed choice regarding immunosuppressive medications would improve more in the decision aid group. Of 301 randomized women, 298 were analyzed; 47% were African-American, 26% Hispanic, and 15% white. Mean age (standard deviation [SD]) was 37 (12) years, 57% had annual income of <$40,000, and 36% had a high school education or less. Compared with the provision of the ACR lupus pamphlet (n = 147), participants randomized to the decision aid (n = 151) had (1) a clinically meaningful and statistically significant reduction in decisional conflict, 21.8 (standard error [SE], 2.5) versus 12.7 (SE, 2.0; p = 0.005) and (2) no difference in informed choice in the main analysis, 41% versus 31% (p = 0.08), but clinically meaningful and statistically significant difference in sensitivity analysis (net values for immunosuppressives positive [in favor] versus negative [against]), 50% versus 35% (p = 0.006). Unresolved decisional conflict was lower in the decision aid versus pamphlet groups, 22% versus 44% (p < 0.001). Significantly more patients in the decision aid versus pamphlet group rated information to be excellent for understanding lupus nephritis (49% versus 33%), risk factors (43% versus 27%), medication options (50% versus 33%; p ≤ 0.003 for all); and the ease of use of materials was higher in the decision aid versus pamphlet groups (51% versus 38%; p = 0.006). Key study limitations were the exclusion of men, short follow-up, and the lack of clinical outcomes, including medication adherence.ConclusionsAn individualized decision aid was more effective than usual care in reducing decisional conflict for choice of immunosuppressive medications in women with lupus nephritis.Trial registrationClinicaltrials.gov, NCT02319525