Spatial variability in condition of southern rock lobsters (Jasus edwardsii) at the start of the Tasmanian fishing season

This research was funded by the Australian Seafood Cooperative Research Centre and the Fisheries Research and Development Corporation (project number 2014/726) (http://www.seafoodcrc.com/) (http://frdc.com.au/Pages/home.aspx). The Australian Research Council's Industrial Transformation Research Program (project number IH120100032) is gratefully acknowledged for providing further financial support for two authors to present this work at the Crustacean Society and International Association of Astacology Mid-year Meeting, 19-23 July 2015 (http://www.arc.gov.au/industrial-transformation-research-programme).The southern rock lobster (Jasus edwardsii) industry in Australia favours red lobsters, which are usually caught in shallow waters, over paler (brindle) lobsters. This preference is driven partly by the Chinese market, where red is associated with luck and prosperity, and additionally, by the widely held perception within the industry that brindles have greater mortality rates during out of water transport than reds. Limited scientific evidence supports these industry observations; however, these studies did not evaluate the initial condition of lobsters. This study aimed first, to determine which variables better describe condition in J. edwardsii and second, to compare condition among lobsters in several sites around Tasmania at the typical time of high transport mortality. Male lobsters were collected from the South West, South East, East and North coast of Tasmania in late November/December 2014, which correspond to the start of the Tasmanian fishing season. A comprehensive condition assessment was applied by measuring tissue proximal composition, Brix index, Total Haemocyte Count, pH, haemocyanin and another 16 haemolymph parameters of interest. A useful framework to compare condition in J. edwardsii was established by first, using Brix index as a measure of nutritional condition, second, using pH, magnesium, and bicarbonate to evaluate differences in physiological condition and finally, using THC counts as a proxy for lobster health condition. Lobsters from different sites had different nutritional, physiological and health condition, consistent with industry observations, however our results indicate that some red shallow water lobsters exhibited poorer nutritional and health condition, while some deep water brindle lobsters were in good condition. Differences in condition could not be directly associated to catch depth of lobsters and was related to other spatially discrete factors which sometimes vary over distances <3 km.Publisher PDFPeer reviewe

Manipulation of Cellular Machinery to Produce Anti-cancer Drugs

The nocardioazine natural products are uniquely prenylated and methylated indole alkaloid diketopiperazines (DKPs) that reverse drug resistance of cancer cell lines. We unveiled the nocardioazine biosynthetic pathway from a marine actinomycete, demonstrating that a cyclodipeptide synthase catalyzes cyclo(L-Trp-L-Trp) DKP precursor formation followed by tailoring of this DKP via a novel racemase, prenyltransferase, and methyltransferase to yield nocardioazine B. These results highlight the aptitude of bacteria for chemical synthesis and offer new enzymatic tools for crafting complex organic molecules

The Good, the Bad and the Recovery in an Assisted Migration

, to enhance depleted populations, improve the yield and sustainability of the fishery, and test resilience to a changing climate. more than lobsters at the source site. Lobsters have isometric growth whereby volume increases as a cube of length. Consequently despite the one-year hiatus in reproduction, increased growth increases fecundity of translocated lobsters, as the increase in size provided a larger volume for producing and incubating eggs in future years.Assisted migration improved egg production and growth, despite a temporary stress response, and offers a tool to improve the production, sustainability and resilience of the fishery

Combining Experiment and Theory to Elucidate the Role of Supercritical Water in Sulfide Decomposition

The cleavage of C–S linkages plays a key role in fuel processing and organic geochemistry. Water is known to affect these processes, and several hypotheses have been proposed, but the mechanism has been elusive. Here we use both experiment and theory to demonstrate that supercritical water reacts with intermediates formed during alkyl sulfide decomposition. During hexyl sulfide decomposition in supercritical water, pentane and CO + CO2 were detected in addition to the expected six carbon products. A multi-step reaction sequence for hexyl sulfide reacting with supercritical water is proposed which explains the surprising products, and quantum chemical calculations provide quantitative rates that support the proposed mechanism. The key sequence is cleavage of one C–S bond to form a thioaldehyde via radical reactions, followed by a pericyclic addition of water to the CS bond to form a geminal mercaptoalcohol. The mercaptoalcohol decomposes into an aldehyde and H2S either directly or via a water-catalyzed 6-membered ring transition state. The aldehyde quickly decomposes into CO plus pentane by radical reactions. The time is ripe for quantitative modelling of organosulfur reaction kinetics based on modern quantum chemistry

Disentangling genetic from environmental effects on phenotypic variability of Southern rock lobster (Jasus edwardsii) postlarvae

Environmental conditions experienced during larval dispersal of marine organisms can determine size-at-settlement of recruits. It is, therefore, not uncommon that larvae undergoing different dispersal histories would exhibit phenotypic variability at recruitment. Here we investigated morphological differences in recently settled southern rock lobster (Jasus edwardsii) recruits, known as pueruli, along a latitudinal and temporal gradient on the east coast of Tasmania, Australia. We further explored whether natural selection could be driving morphological variation. We used double digest restriction-site associated DNA sequencing (ddRADseq) to assess differences in genetic structure of recently settled recruits on the east coast of Tasmania over three months of peak settlement during 2012 (August, September and October). Phenotypic differences in pueruli between sites and months of settlement were observed, with significantly smaller individuals found at the northernmost site. Also, there was a lack of overall genetic divergence; however, significant differences in pairwise FST values between settlement months were observed at the southernmost study site, located at an area of confluence of ocean currents. Specifically, individuals settling into the southernmost earlier in the season were genetically different from those settling later. The lack of overall genetic divergence in the presence of phenotypic variation indicates that larval environmental history during dispersal of J. edwardsii could be a possible driver of the resulting phenotype of settlers

Temporal genetic patterns of diversity and structure evidence chaotic genetic patchiness in a spiny lobster

Population structure of many marine organisms is spatially patchy and varies within and between years, a phenomenon defined as chaotic genetic patchiness. This results from the combination of planktonic larval dispersal and environmental stochasticity. Additionally, in species with bi-partite life, postsettlement selection can magnify these genetic differences. The high fecundity (up to 500,000 eggs annually) and protracted larval duration (12–24 months) and dispersal of the southern rock lobster, Jasus edwardsii, make it a good test species for chaotic genetic patchiness and selection during early benthic life. Here, we used double digest restriction site-associated DNA sequencing (ddRADseq) to investigate chaotic genetic patchiness and postsettlement selection in this species. We assessed differences in genetic structure and diversity of recently settled pueruli across four settlement years and between two sites in southeast Australia separated by approximately 1,000 km. Postsettlement selection was investigated by identifying loci under putative positive selection between recently settled pueruli and postpueruli and quantifying differences in the magnitude and strength of the selection at each year and site. Genetic differences within and among sites through time in neutral SNP markers indicated chaotic genetic patchiness. Recently settled puerulus at the southernmost site exhibited lower genetic diversity during years of low puerulus catches, further supporting this hypothesis. Finally, analyses of outlier SNPs detected fluctuations in the magnitude and strength of the markers putatively under positive selection over space and time. One locus under putative positive selection was consistent at both locations during the same years, suggesting the existence of weak postsettlement selection

Using wastewater-based epidemiology to estimate drug consumption—Statistical analyses and data presentation

Aim Analysis of wastewater samples can be used to assess population drug use, but reporting and statistical issues have limited the utility of the approach for epidemiology due to analytical results that are below the limit of quantification or detection. Unobserved or non-quantifiable—censored—data are common and likely to persist as the methodology is applied to more municipalities and a broader array of substances. We demonstrate the use of censored data techniques and account for measurement errors to explore distributions and annual estimates of the daily mean level of drugs excreted per capita. Measurements Daily 24-hour composite wastewater samples for 56 days in 2009 were obtained using a random sample stratified by day of week and season for 19 municipalities in the Northwest region of the U.S. Methods Methamphetamine, benzoylecgonine (cocaine metabolite), 3,4-ethylenedioxymethamphetamine (MDMA), methadone, oxycodone and hydrocodone were identified and quantified in wastewater samples. Four statistical approaches (reporting censoring, maximum likelihood estimation, Kaplan-Meier estimates, or complete data calculations) were used to estimate an annual average, including confidence bounds where appropriate, dependent upon the amount of censoring in the data. Findings The proportion of days within a year with censored data varied greatly by drug across the 19 municipalities, with MDMA varying the most (4% to 94% of observations censored). The different statistical approaches each needed to be used given the levels of censoring of measured drug concentrations. Figures incorporating confidence bounds allow visualization of the data that facilitates appropriate comparisons across municipalities. Conclusions Results from wastewater sampling that are below detection or quantification limits contain important information and can be incorporated to create a more complete and valid estimate of drug excretion

The first interferometric detections of Fast Radio Bursts

We present the first interferometric detections of Fast Radio Bursts (FRBs), an enigmatic new class of astrophysical transient. In a 180-day survey of the Southern sky we discovered 3 FRBs at 843 MHz with the UTMOST array, as part of commissioning science during a major ongoing upgrade. The wide field of view of UTMOST ($\approx 9$ deg$^{2}$) is well suited to FRB searches. The primary beam is covered by 352 partially overlapping fan-beams, each of which is searched for FRBs in real time with pulse widths in the range 0.655 to 42 ms, and dispersion measures $\leq$2000 pc cm$^{-3}$. Detections of FRBs with the UTMOST array places a lower limit on their distances of $\approx 10^4$ km (limit of the telescope near-field) supporting the case for an astronomical origin. Repeating FRBs at UTMOST or an FRB detected simultaneously with the Parkes radio telescope and UTMOST, would allow a few arcsec localisation, thereby providing an excellent means of identifying FRB host galaxies, if present. Up to 100 hours of follow-up for each FRB has been carried out with the UTMOST, with no repeating bursts seen. From the detected position, we present 3$\sigma$ error ellipses of 15 arcsec x 8.4 deg on the sky for the point of origin for the FRBs. We estimate an all-sky FRB rate at 843 MHz above a fluence $\cal F_\mathrm{lim}$ of 11 Jy ms of $\sim 78$ events sky$^{-1}$ d$^{-1}$ at the 95 percent confidence level. The measured rate of FRBs at 843 MHz is of order two times higher than we had expected, scaling from the FRB rate at the Parkes radio telescope, assuming that FRBs have a flat spectral index and a uniform distribution in Euclidean space. We examine how this can be explained by FRBs having a steeper spectral index and/or a flatter log$N$-log$\mathcal{F}$ distribution than expected for a Euclidean Universe.Comment: 13 pages, 8 figures, 2 table

The UTMOST pulsar timing programme I: overview and first results

We present an overview and the first results from a large-scale pulsar timing programme that is part of the UTMOST project at the refurbished Molonglo Observatory Synthesis Radio Telescope (MOST) near Canberra, Australia. We currently observe more than 400 mainly bright southern radio pulsars with up to daily cadences. For 205 (8 in binaries, 4 millisecond pulsars) we publish updated timing models, together with their flux densities, flux density variability, and pulse widths at 843 MHz, derived from observations spanning between 1.4 and 3 yr. In comparison with the ATNF pulsar catalogue, we improve the precision of the rotational and astrometric parameters for 123 pulsars, for 47 by at least an order of magnitude. The time spans between our measurements and those in the literature are up to 48 yr, which allows us to investigate their long-term spin-down history and to estimate proper motions for 60 pulsars, of which 24 are newly determined and most are major improvements. The results are consistent with interferometric measurements from the literature. A model with two Gaussian components centred at 139 and $463~\text{km} \: \text{s}^{-1}$ fits the transverse velocity distribution best. The pulse duty cycle distributions at 50 and 10 per cent maximum are best described by log-normal distributions with medians of 2.3 and 4.4 per cent, respectively. We discuss two pulsars that exhibit spin-down rate changes and drifting subpulses. Finally, we describe the autonomous observing system and the dynamic scheduler that has increased the observing efficiency by a factor of 2-3 in comparison with static scheduling.Comment: 31 pages, 14 figures, 6 tables, accepted for publication in MNRA