Avaliação de nematoides entomopatogênicos em condições de laboratório e casa-de-vegetação visando ao controle de Spodoptera frugiperda

The increased corn planting grown in the late season crop and the use of a notillage planting system benefits the development of Spodoptera frugiperda, the main pest of corn crop. Thus, this study aimed to select entomopathogenic nematodes based on the mortality of S. frugiperda, and to adjust the nematode concentrations in the laboratory and evaluate the action of these organisms in greenhouse conditions. Seventeen nematode populations were tested at concentrations of 100, 250, and 500 infective juveniles (IJ)/larvae. The obtained data were submitted to the polynomial regression test. In the greenhouse, corn sowing was performed in plastic buckets with soil, corn straw, and water. After 7 days, Steinernema arenarium or Heterorhabditis sp. RSC02, and the larvae were added (40 larvae, one larvae/repetition). The data were submitted to the variance analysis and the Scott-Knott test (P<0.05). In the laboratory, tests with S. arenarium and Heterorhabditis sp. RSC02 caused mortality of 85 and 90%, respectively, being selected for the concentrations test. Concentrations of 150, 200, 250, 300, and 350IJ larvae-1 were tested. At a concentration of 200IJ larvae-1, S. arenarium and Heterorhabditis sp. RSC02 caused 100 and 97.6% mortality in laboratory, respectively, and in the greenhouse caused 77.5 and 87.5% mortality, respectively, when compared to the control (7.5%).O aumento no plantio do milho "safrinha" e o uso do sistema de plantio direto favorecem o desenvolvimento de Spodoptera frugiperda, principal praga da cultura. Dessa forma, os objetivos deste trabalho foram selecionar nematoides entomopatogênicos, de acordo com a mortalidade causada sobre S. frugiperda, adequar as concentrações em laboratório e avaliar a ação desses organismos em condições de casa-de-vegetação. Foram testadas 17 populações de nematoides nas concentrações de 100, 250 e 500 juvenis infectantes (JI) lagarta-1. Os dados obtidos foram submetidos ao teste de regressão polinomial. Em casa-de-vegetação, a semeadura do milho foi realizada em vasos de plástico contendo solo, palhada de milho e água. Após sete dias, foram adicionados Steinernema arenarium ou Heterorhabditis sp. RSC02 e as lagartas (40 lagartas, uma lagarta/repetição). Os dados obtidos foram submetidos à análise de variância e ao teste de médias Scott-Knott (P<0,05). Nos testes de laboratório, S. arenarium e Heterorhabditis sp. RSC02 causaram mortalidade de 85 e 90%, respectivamente, sendo selecionados para o teste de concentrações. Foram testadas as concentrações de 150, 200, 250, 300 e 350JI lagarta-1. Na concentração de 200JI lagarta-1, S. arenarium e Heterorhabditis sp. RSC02 causaram 100 e 97,6% de mortalidade, respectivamente, e em casa-de-vegetação causaram 77,5 e 87,5%, respectivamente, diferindo da testemunha (7,5%)

FLUTUAÇÃO POPULACIONAL DE CRISOPÍDEOS (NEUROPTERA: CHRYSOPIDAE) EM POMARES DE ABACATE (PERSEA AMERICANA)

Crisopídeos são insetos pertencentes a ordem Neuroptera. Os adultos se alimentam de pólen, néctar e honeydew secretado por alguns hemípteros. São predadores em sua fase larval sendo encontrados em várias culturas de interesse económico, possuindo como presas principais lagartas neonatas, pulgões, cochonilha, moscasbrancas, psilídeos, tripes e ácaros, contribuindo dessa forma no controle biológico natural de pragas em ambientes agrícolas. Este trabalho teve como objetivo acompanhar a flutuação populacional de crisopídeos em pomares de abacate (Percea americana) na fazenda Santa Cecilia, do grupo Carlini Avocados, no município de Bernardino de Campos, estado de São Paulo. Os monitoramentos foram realizados semanalmente no período de agosto de 2020 até agosto de 2022, em 62 talhões, de diferentes tamanhos das variedades Margarida, Fortuna, Quintal e Hass, com idade igual ou superior a três anos. Para isso, semanalmente foram avaliados 5 pontos de amostragem em áreas de até 5 hectares e 10 pontos em áreas superiores a 5 hectares. O caminhamento realizado foi em zigue-zague, alternando os pontos monitorados entre semanas pares e ímpares. O ano de 2021 foi um ano atípico para a cultura do abacate, associado com fatores climáticos atípicos, como as fortes geadas, diminuindo a incidências de floradas das gramíneas e outras plantas espontâneas, o que pode ter contribuído para a diminuição na população de crisopídeos. O aumento de indivíduos no ano de 2022, esteve diretamente relacionado ao clima, presença de gramíneas, além da própria florada da cultura.&nbsp;&nbsp

Taxonomic studies of laelapid mites (Acari: Mesostigmata: Laelapidae) and their use in combination with entomopathogenic nematodes (Rhabditida: Steinernematidae, Heterorhabditidae) to control Frankliniella occidentalis (Thysanoptera: Thripidae)

A família Laelapidae Berlese (Mesostigmata: Dermanyssoidea) é cosmopolita e ocupa uma diversidade de habitats, onde seus hábitos podem variar de espécies de vida-livre a espécies associadas a artrópodes, mamíferos e pássaros. Algumas espécies dessa família, bem como os nematoides entomopatogênicos (NEP) têm demonstrado bom potencial como agentes de controle biológico. Frankliniella occidentalis (Pergande), uma das espécies mais importantes de tripes-praga, causa danos a várias plantas cultivadas, atuando como praga ou vetor de patógenos. Os objetivos desse estudo foram a preparação de um catálogo taxonômico para lelapídeos de vida-livre e associados a artrópodes, o estudo taxonômico de espécies de Laelapidae coletados no Brasil, a preparação de uma chave para gêneros de Laelapidae de vida livre e associados a artrópodes até o momento conhecidos da América do Sul, e avaliar os efeitos de diferentes espécies de lelapídeos e NEP como agentes de controle de F. occidentalis. Um total de 828 espécies pertencentes a 41 gêneros são relatados no catálogo. Nove espécies novas foram descritas e uma chave para gêneros de ocorrência na América do Sul de Laelapidae foi elaborada. Em testes de laboratório, Cosmolaelaps jaboticabalensis Moreira, Klompen & Moraes e Stratiolaelaps scimitus (Womersley) predaram aproximadamente 2,5 e 3,9 pré-pupa/pupa de F. occidentalis e ovipositaram aproximadamente 2,2 e 1,4 ovos por dia, respectivamente. Heterorhabditis amazonensis GL Andaló, Nguyen & Moino Jr. foi o nematoide mais virulento, causando a mortalidade de aproximadamente 87,5% das pre-pupas/pupas oferecidas. Nenhum dos predadores avaliados mostrou preferência por este nematoide como presa e nenhum dos NEP avaliados interferiram na sobrevivência dos predadores. Para C. jaboticabalensis, a fase imatura (ovo-adulto) foi completada em 12,3 ± 5; 6,6 ± 0,6 e 7,1 ± 0,6 em F. occidentalis, Protorhabditis sp. ...The cosmopolitan Dermanyssoidea mite family Laelapidae Berlese occupies a diversity of habitats, where their habits vary from free-living to arthropod, mammal and bird associates. Some species of this family as well the entomopathogenic nematodes (EPN) have shown good potential as biological control agents. Frankliniella occidentalis (Pergande), one of the most important thrips pests, causes major damage to several cultivated plants, acting as pest or vector of pathogens. The aims of the study reported in this document were the preparation of a taxonomic catalog of the free-living and arthropod associated laelapid mites, the taxonomic study of the laelapid species collected in Brazil, the preparation of a key for the South American free-living and arthropod associated genera, and evaluations of the effect of different laelapid and EPN species as control agents of F. occidentalis. A total of 828 species of 41 genera are reported in the catalog. Nine new species were described and a key for the separation of genera reported in South America genus was prepared. In laboratory tests, Cosmolaelaps jaboticabalensis Moreira, Klompen and Moraes and Stratiolaelaps scimitus (Womersley) preyed upon about 2.5 and 3.9 pre-pupae/pupae of F. occidentalis and laid about 2.2 and 1.4 eggs per day, respectively. Heterorhabditis amazonensis GL Andaló, Nguyen and Moino Jr. was the most virulent nematode, producing mortality of about 87.5% of exposed pre-pupae/pupae. None of the evaluated predators showed preference for that nematode as prey and none of the evaluated nematodes interfered in the survivorship of the predators. For C. jaboticabalensis, total immature development (egg-adult) was completed in 12.3 ± 5, 6.6 ± 0.6 and 7.1 ± 0.6 on F. occidentalis, Protorhabditis sp. and T. putrescentiae, respectively. Fecundity and intrinsic rate of increase were higher on Protorhabditis sp. (71.6 ± 9.1 eggs/female; 0.28 female/female/day) than ..

Redefinition of Cosmolaelaps Berlese (Acari: Laelapidae) and description of five new species from Brazil

Moreira, Grazielle Furtado, Klompen, Hans, Moraes, Gilberto José De (2014): Redefinition of Cosmolaelaps Berlese (Acari: Laelapidae) and description of five new species from Brazil. Zootaxa 3764 (3): 317-346, DOI: 10.11646/zootaxa.3764.3.

FIGURE 1 in Redefinition of Cosmolaelaps Berlese (Acari: Laelapidae) and description of five new species from Brazil

FIGURE 1. Cosmolaelaps barbatus. Female. Dorsal idiosoma.Published as part of Moreira, Grazielle Furtado, Klompen, Hans & Moraes, Gilberto José De, 2014, Redefinition of Cosmolaelaps Berlese (Acari: Laelapidae) and description of five new species from Brazil, pp. 317-346 in Zootaxa 3764 (3) on page 324, DOI: 10.11646/zootaxa.3764.3.4, http://zenodo.org/record/23087

Cosmolaelaps busolii Moreira, Klompen & Moraes, 2014, n. sp.

Cosmolaelaps busolii n. sp. Material examined: holotype female and one paratype female collected on 19 April 2000 by A.R. Oliveira from soil under Syagrus romanzoffiana (Cham) (Arecaceae) at Rodovia 226 (Parquera-Açu-Cananéia), km 16, Cananéia, São Paulo state, Brazil; one paratype male collected on 12 October 2000 by A.R. Oliveira from litter under Astrocaryum aculeatissimum (Schott) (Arecaceae) at Estação Experimental do IAC, Pariqueraçu, São Paulo state, Brazil; holotype and paratypes deposited at ESALQ-USP. Diagnosis: female dorsal shield with 39 pairs of setae and two unpaired setae, all scimitar-shaped, except j 1, setiform. Unsclerotised lateroventral cuticle with eight pairs of setae. Anterior lobes of sternal shield not distinguishable, sternal shield lightly sclerotised, with indistinct anterior margin. Genital shield bearing only st 5. Two pairs of metapodal plates. Ten pairs of setiform opisthogastric setae (Jv 1 –Jv 5; Zv 1 –Zv 5). Epistome convex, denticulate. Fixed cheliceral digit with five teeth in addition to apical tooth. Female (Figure 4 A–F) Dorsal idiosoma (Fig. 4 A): dorsal shield 413 (399–427) long and 263 (255–271) wide (at level of r 6), reticulate, with most reticules transversely elongate, with 39 pairs of setae, including two pairs of extra setae, and two unpaired setae [j 1 –j 6, z 1 –z 6, s 1 –s 6, r 2 –r 5 on podonotal region of dorsal shield and J 1 –J 5, Z 1 –Z 5, S 1 –S 5, px 2 (posterolaterad of J 2) and px 3 (posterolad of J 3) and two unpaired Jx setae (in transverse line with J 2 and in transverse line with px 3) on opisthonotal region of dorsal shield]. Setae scimitar-shaped (with basal protuberance facing axis of the idiosoma), except j 1, setiform. Length of setae shown in Table 1. Dorsal shield with 17 and six pairs of distinguishable lyrifissures and pores respectively. Unsclerotised lateroventral cuticle with eight pairs of setae (r 6, R 1, R 2, R 4 –R 6, two UR setae). Peritreme (Fig. 4 F): peritrematic shield only fused to dorsal shield, near z 1, with a pair of lyrifissures and a pore behind stigma; with a pore and a lyrifissure dorsad of peritreme between coxae II and III. Peritreme extending anteriorly to level between s 1 and z 1. Ventral idiosoma (Fig. 4 B): anterior lobes of sternal shield not distinguishable; sternal shield lightly sclerotised, smooth, with indistinct anterior margin; posterior margin only distinguishable near st 3; about as long as wide, with three pairs of setae (st 1 –st 3) and two pairs of lyrifissures (iv 1, iv 2); distance between st 1 and the posterior margin of sternal shield 80 (79–81), st 1 –st 1 58 (58–59), st 2 –st 2 70 (69–71), st 3 –st 3 87 (87 – 87). Length of setae shown in Table 2. Seta st 4 on unsclerotised cuticle, posterolaterad of iv 3 which is also on cuticle. Genital shield not fused with anal shield, flask-shaped, reticulate [length 190 (190 – 190), width 84 (82–87)], with one pair of setae (st 5); distance st 5 –st 5 98 (89–107). Genital lyrifissure (iv 5) on unsclerotised cuticle, posterolatered of st 5. Rod-shaped plate fused with genital shield by anterior end and with acute posterior end free. Two pairs of metapodal plates, the anterior smaller. Anterior section of endopodal shield distinctly more sclerotised than sternal shield, fused with it, with anterior end distinctly prolonged between coxae I and II; section behind sternal shield fragmented into a boomerang-shaped fragment between coxae III and IV and a smaller rod-shaped posterior fragment surrounding part of postero-mesad region of coxa IV. Exopodal shield indistinct. Anal shield subtriangular (wider section anterior), with scanty striae [65 (63–66) long and 63 (61–65) wide], with a pair of marginal pores about in transverse line with anterior margin of anal opening. With 10 pair of setiform opisthogastric setae: Jv 1 –Jv 5, Zv 1 –Zv 5; with a minute plate anterolaterad of Zv 1. Gnathosoma: epistome convex, denticulate (Fig. 4 D). Deutosternum with a smooth, transverse distal line followed by six transverse lines of denticles; number of teeth decreasing from distal (26) to proximal row (8); with a pair of smooth, transverse and curved lines external to deutosternum, between second and third most proximal lines of denticles (Fig. 4 C). Corniculi parallel to each other, 27 (25–28) long and 7 (6–7) wide basally, almost reaching middle of palp femur. Internal malae adjacent to each other, smooth, laterad by a pair of structures subdivided distally into about five coarse tines. Lengths of setae on venter of gnathosoma shown in Table 2. Hypostomal seta h 2 laterad and slightly anteriad of h 3. Palp length 115 (113–118), apotele 2 -tined; inner palp trochanter seta about 1.6 times as long as outer seta. Cheliceral digits of similar lengths (Fig. 4 E); movable cheliceral digit 36 (36–37) with two teeth in addition to distal tooth; fixed digit 41 (40–42) with five teeth in addition to apical tooth and minute setiform pilus dentilis. Stout dorsal cheliceral seta and dorsal lyrifissure distinguishable; antiaxial lyrifissure indistinguishable. Cheliceral arthrodial process as a coronet-like fringe. Legs: lengths, not including ambulacra—I, 412 (411–413); II, 320 (318–321); III, 310 (308–312); IV, 436 (425–448); chaetotaxy: I—coxa 0-0/ 2, 0/0-0, trochanter 1 -0/ 1, 1 / 2 - 1, femur 2 - 2 / 1, 3 / 3 - 2, genu 2-3 / 2, 3 / 1-2, tibia 2- 3 / 2, 3 / 1-2; II—coxa 0-0/ 1, 0/ 1 -0, trochanter 1 -0/ 1, 1 / 1 - 1, femur 2-3 / 1, 2 / 2 - 1, genu 2-3 / 1, 2 / 1-2, tibia 2 - 2 / 1, 2 / 1-2; III—coxa 0-0/ 1, 0/ 1 -0, trochanter 1 - 1 / 1, 0/ 1 - 1, femur 1-2 / 1, 1 / 0-1, genu 2 - 2 / 1, 2 / 1 - 1, tibia 2 - 1 / 1, 2 / 1 - 1; IV- coxa 0- 0/ 1, 0/0-0, trochanter 2 - 1 / 1, 0/ 1 -0, femur 1-2 / 1, 1 / 0-1, genu 2 - 2 / 1, 3 / 0-1, tibia 2 - 1 / 1, 3 / 1-2; tarsi II–IV: 18 setae each. Pre-tarsi with short claws, pulvillus distally rounded. Setae uniform in shape, no macrosetae. Male (Figure 4 G–H) Dorsal idiosoma: dorsal shield [318 long and 196 wide (at level of r 6)], similar in reticulation and setation to female. Dorsal setal length about 65 % of that in female (Table 3). Unsclerotised lateral cuticle with seven pairs of setae (r 6, R 1, R 2, R 4 -R 6, one UR). Peritreme: as in female. Ventral idiosoma (Fig. 4 H): holoventral shield [259 long and 150 of wide (behind coxae IV)], with ten pairs of setae (st 1 –st 5, Jv 1 –Jv 3, Zv 1 and Zv 2) in addition to circumanal setae, a single pair of barely distinguishable lyrifissures (iv 2) and a pair of marginal pores about in transverse line with posterior margin of anal opening. Holoventral setae about 60 % as long as in female (Table 4). Unsclerotised cuticle posterolaterad of ventrianal region with five pairs of setae (Jv 4, Jv 5, Zv 3 –Zv 5) and no distinguishable lyrifissures. Gnathosoma: epistome, deutosternum and corniculi similar to female. Cheliceral digits of similar lengths (Fig. 4 G); movable digit 29 with two teeth in addition to distal tooth; fixed digit 33, with one tooth distal to minute setiform pilus dentilis; spermatodactyl 37, curved upward, apparently widely fused basally with movable digit. Dorsal cheliceral seta and lyrifissure distinguishable; antiaxial lyrifissure indistinct. Legs: as in female. Etymology. the name busolii is a homage to Antonio Carlos Busoli, for his contribution to Brazilian entomology. Remarks. Cosmolaelaps busolii is closely related to C. panniculus (Karg) and C. paulista (Freire & Moraes), both described from Brazil. They have dorsal shield setae scimitar-shaped, Z 5 shorter than other dorsal shield setae, epistome convex, bearing denticles of similar lengths. Cosmolaelaps panniculus differs from C. busolii by having only 35 pairs of dorsal shield setae, and the sternal shield normally sclerotised, reticulate. Cosmolaelaps paulista differs by having setae of the podonotal region of the dorsal shield usually distinctly longer than the distance to the subsequent setae in the same series, especially j 5, j 6 and z 2; sternal shield normally sclerotised, reticulate; genital shield smooth near its posterior end, and the exopodal shield behind the sternal shield consisting of a single fragment extending from the region between coxae III–IV to the region behind coxa IV.Published as part of Moreira, Grazielle Furtado, Klompen, Hans & Moraes, Gilberto José De, 2014, Redefinition of Cosmolaelaps Berlese (Acari: Laelapidae) and description of five new species from Brazil, pp. 317-346 in Zootaxa 3764 (3) on pages 331-333, DOI: 10.11646/zootaxa.3764.3.4, http://zenodo.org/record/23087

Heterorhabditis amazonensis RSC5 (Rhabditida: Heterorhabditidae) movement and host recognition

Response of Heterorhabditis amazonensis RSC5 to compounds released by different host insects and its virulence level to several insect hosts like Galleria mellonella, Mycotretus apicalis and Tenebrio molitor were evaluated in this study, and compared with other entomopathogenic nematode species like Steinernema carpocapsae All and Steinernema riobrave 355. Tests were performed in Petri dishes with agar-water 2% to determine nematode movement toward the insect with and without opportunity of choosing different insect hosts. Evaluations were made quantifying the proximity of infective juveniles (IJs) to the insect as a source of allurement. In order to determine the displacement of IJs in a closed soil condition, a test was carried out in an arena with sand. The nematode was virulent to the target insects. When nematode and insect were released on agar-water, IJs moved toward the stimulus, with H. amazonensis showing preference for certain insects. In the arena with sand S. carpocapsae caused lower insect mortality (70% +/- 8.9 for G. mellonella) than H. amazonensis and S. riobrave (80% +/- 6.5 and 99% +/- 0.0). Heterorhabditis amazonensis was able to find and choose its hosts (G. mellonella and T. molitor), similarly to S. riobrave behavior, and located them more effectively than S. carpocapsae. The virulence of H. amazonensis was thus similar to S. riobrave, and this characteristic could be promising to introduce this native species in integrated pest management programs.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Cosmolaelaps Berlese

Cosmolaelaps Berlese Laelaps (Cosmolaelaps) Berlese, 1903: 13. Cosmolaelaps.— Berlese, 1920: 157; Vitzthum, 1929: 22; Bregetova, 1956: 71; Sheals, 1962: 107; Casanueva, 1993: 40; Hunter, 1993: 23; Botelho et al., 2002: 54. Hypoaspis (Cosmolaelaps).— Vitzthum, 1941: 762; Schweizer, 1961: 149; Evans & Till, 1966: 160; Bregetova, 1977: 511; Karg, 1979: 71 (in part); Karg, 1981: 213 (in part); Karg, 1988: 509 (in part); Hafez et al., 1982: 3; Karg, 1982: 234; Fouly et al., 1997: 33; Huhta & Karg, 2010: 330 (in part). Type species. Laelaps claviger Berlese, 1883: 2. The most detailed diagnosis of Cosmolaelaps was provided by Evans & Till (1979) in a key to genera of Laelapidae. According to that key, species of this genus have the dorsal shield setae spatulate, cuneiform or scimitar-shaped; opisthonotal region of dorsal shield with one or two pairs of px (referred to as Zx) setae and a total of at least 16 pairs of setae; corniculi relatively short; female genital and anal shields not fused with other shields; palp apotele 2 -tined; genu IV typically with nine setae (pl 2 absent); chelicerae chelate-dentate and cheliceral digits of similar lengths; metasternal setae not inserted on sternal shield and spermatodactyl free. The following species are considered in this publication to belong to Cosmolaelaps, and have been considered in the updated diagnosis of the genus to be presented below: C. aciphilus (Karg, 1987) n. comb. [described as Hypoaspis (C.) aciphila]; C. acutiscutus Teng, 1982; C. alexandrini (Fox, 1946) [described as Ischnolaelaps alexandrini, transferred to Cosmolaelaps by Freire & Moraes (2007)]; C. anserinus (Karg, 1981) [described as H. (C.) anserina, transferred to Cosmolaelaps by Farrier & Hennessey (1993)]; C. barbatus n.sp.; C. bengalensis (Bhattacharyya, 1968) n. comb. [described as H. bengalensis]; C. bicuspisetosus Willmann, 1953; C. bipennatus (Karg, 2003) n. comb. [described as H. (C.) bipennata]; C. bispinosus (Karg, 1997) n. comb. [described as H. (C.) bispinosa]; C. brevilingua (Karg, 2006) n. comb. [described as H. (C.) brevilingua]; C. brevipedestrus (Karg, 1985) [described as H. (C.) brevipedestra, transferred to Cosmolaelaps by Farrier & Hennessey (1993)]; C. brevistilis (Karg, 1978) n. comb. [described as H. (C.) brevistilis]; C. burdwanensis (Bhattacharyya, 1972) n. comb. [described as H. burdwanensis]; C. busolii n.sp.; C. calamitus (Faraji & Halliday, 2009) n. comb. [described as H. (C.) calamitus]; C. carvalhoi (Aswegen & Loots, 1970) n. comb. [described as H. (H.) carvalhoi]; C. cassoidea (Karg, 1981) [described as H. (C.) cassoidea, transferred to Cosmolaelaps by Freire & Moraes (2007)]; C. chenchuanhoi (Samši&ncaron;ák, 1964) n. comb. [described as Stratiolaelaps chenchuanhoi]; C. chianensis (Gu, 1990) n. comb. [described as H. chianensis; senior synonym of H. (C.) hefeiensis Xu & Liang, 1996, according to Ma (2006)]; C. chini Bai & Gu, 1993; C. claviger (Berlese, 1883) [described as Laelaps claviger, transferred to Cosmolaelaps by Hull (1918); senior synonym of C. bregetovae Piryanik, 1959, according to Bregetova (1977); of H. (H.) auris Karg, 1965, according to Costa (1968); of H. (C.) macroanalis Bernhard in Hirschmann et al., 1969, according to Karg (1981)]; C. communis Trägårdh, 1931; C. confinisetarum n.sp.; C. crossocauda (Karg, 2006) n. comb. [described as H. (C.) crossocauda]; C. cubaensis (Karg, 1981) [described as H. (C.) cubaensis, transferred to Cosmolaelaps by Farrier & Hennessey (1993)]; C. cuneifer (Michael, 1891) [described as L. cuneifer, transferred to Cosmolaelaps by Hull (1918)]; C. cursorius (Karg, 1988) [described as H. (C.) cursoria, transferred to Cosmolaelaps by Farrier & Hennessey (1993)]; C. dendrophilus Davydova, 1977; C. diversichaetatus Grochovskaya & Huan-Hoe, 1961; C. diversus (Karg, 1994) n. comb. [described as H. (C.) diversa]; C. euarmatus (Karg, 1996) n. comb. [described as H. (C.) euarmata]; C. eucapillatus (Karg, 2003) n. comb. [described as H. (C.) eucapillata]; C. euventricosus (Karg, 1995) n. comb. [described as H. (C.) euventricosa]; C. furcatoides (Karg, 1981) n. comb. [described as H. (C.) furcatoides]; C. gladii (Karg, 1993 b) n. comb. [described as H. (C.) gladii]; C. guoi (Bai & Ma, 2012) n. comb. [described as H. (C.) guoi]; C. guttulatus (Karg, 1978) n. comb. [described as H. (C.) guttulata]; C. hortensis (Ishikawa, 1986) n. comb. [described as H. (C.) hortensis]; C. hrdyi (Samši&ncaron;ák, 1961) {described as H. (C.) hrdýi [sic], transferred to Cosmolaelaps by Ma (2005 a); senior synonym of H. (C.) shenyangensis Bei, Shi & Yin, 2003, according to Ma (2006)}; C. inarmatus (Karg, 1997) n. comb. [described as H. (C.) inarmata]; C. indicus Bhattacharyya, 1966 [= H. calcuttaensis Bhattacharyya, 1971, unjustified replacement name]; C. jaboticabalensis n.sp.; C. kassaii (Aswegen & Loots, 1970) n. comb. [described as H. (H.) kassai]; C. keni (Hafez, ElBadry & Nasr, 1982) [described as H. (C.) keni, transferred to Cosmolaelaps by Zaher, 1986]; C. leeae (Tseng, 1977) n. comb. [described as H. leeae]; C. leptochaetes Ma & Lin, 2009; C. lepoauris (Karg, 1981) n. comb. [described as H. (C.) lepoauris]; C. leptolingua (Karg, 1994) n. comb. [described as H. (C.) leptolingua]; C. liae Bai & Gu, 1993; C. lingua (Karg, 1987) n. comb. [described as H. (C.) lingua]; C. lisimilis Ma, 2007; C. longanalis (Karg, 2003) n. comb. [described as H. (C.) longanalis]; C. longocrinitus (Karg, 2006) n. comb. [described as H. (C.) longocrinita]; C. longodigiti (Karg, 1979) n. comb. [described as H. (C.) longodigiti]; C. longogenitalis (Karg, 1978) n. comb. [described as H. (C.) longogenitalis]; C. longus (Hafez, ElBadry & Nasr, 1882) [described as H. (C.) longus, transferred to Cosmolaelaps by Zaher, 1986]; C. lutegiensis Shcherbak, 1971 a; C. mabilogus Rosario, 1981; C. machadoi (Aswegen & Loots, 1970) n. comb. [described as H. (H.) machadoi]; C. machaeratus (Domrow, 1957) [described as Haemolaelaps machaeratus]; C. macrochaetus (Karg, 1988) [described as H. (C.) macrochaeta, transferred to Cosmolaelaps by Farrier & Hennessey (1993)]; C. mahuncai (Karg, 1988) [described as H. (C.) mahuncai, transferred to Cosmolaelaps by Farrier & Hennessey (1993)]; C. malakutsilyus Rosario, 1981; C. masculinus (Karg, 1988) [described as H. (C.) masculina, transferred to Cosmolaelaps by Farrier & Hennessey (1993)]; C. mediocuspis (Karg, 1981) n. comb. [described as H. (C.) mediocuspis]; C. michaeli (Huhta & Karg, 2010) n. comb. [described as H. (C.) michaeli]; C. mixta (Shcherbak, 1971 b) n. comb. [described as H. mixta]; C. multidentatus (Aswegen & Loots, 1970) n. comb. [described as H. (H.) multidentatus]; C. multisetosus Domrow, 1957; C. nasosetus (Karg, 1981) [described as H. (C.) nasoseta, transferred to Cosmolaelaps by Farrier & Hennessey (1993)]; C. neocuneifer (Evans & Till, 1966) n. comb. [described as H. (C.) neocuneifer]; C. ningxiaensis Bai & Gu, 1993; C. oliveirai n.sp.; C. ornatus (Berlese, 1903) [described as L. (C.) ornatus, transferred to Cosmolaelaps by Hull (1918)]; C. pahabaeus Rosario, 1981; C. panniculus (Karg, 1981) n. comb. [described as H. (C.) pannicula]; C. paracuneifer (Gu & Bai, 1992) n. comb. [described as H. paracuneifer]; C. paravacua Nasr & Nawar, 1989; C. parvidentis (Karg & Schorlemmer, 2009) n. comb. [described as H. (C.) parvidentis]; C. paulista Freire & Moraes, 2007; C. penicillatus (Karg, 1979) n. comb. [described as H. (C.) penicillata]; C. pugiocuspis (Karg, 1981) n. comb. [described as H. (C.) pugiocuspis]; C. pumili (Karg, 1988) [described as H. (C.) pumili, transferred to Cosmolaelaps by Farrier & Hennessey (1993)]; C. punyalus Rosario, 1981; C. qinghaiensis (Li, Yang & Yue, 1997) n. comb. [described as H. qinghaiensis]; C. quasiclaviger Ma & Lin, 2009; C. rectangularis Sheals, 1962; C. reticulatus (Xu & Liang, 1996) [described as H. (C.) reticulatus, transferred to Cosmolaelaps by Ma (2005 b)]; C. retirugi Ma, Yang & Zhang, 2004; C. robustochaetes Ma & Lin, 2009; C. robustoventralis Ma, 2011; C. robustus (Berlese, 1905) [described as L. (C.) robustus, transferred to Cosmolaelaps by Bregetova (1956)]; C. serratus Trägårdh, 1952; C. simplex Berlese, 1920; C. sorecis (Li, Zheng & Yang, 1996) n. comb. [described as H. sorecis]; C. subacutiscutus Bai & Wang, 2005; C. subpictus (Gu & Bai, 1992) n. comb. [described as H. (C.) subpictus]; C. sungaris (Ma, 1996) [described as H. sungaris, transferred to Cosmolaelaps by Ma (2005)]; C. tetraspinae (Karg, 1995) n. comb. [described as H. (C.) tetraspinae]; C. thysanifer Zeman, 1982; C. transvaalensis Ryke, 1963; C. trifidus (Pearse, Patterson, Rankin & Wharton, 1936) [described as Seiodes trifidus, transferred to Cosmolaelaps by Delfinado & Baker (1975); senior synonym of C. passali Hunter & Mollin, 1964, according to Delfinado & Baker (1975)]; C. tuberculatus (Mašán, 1992) n. comb. [described as H. (C.) tuberculata]; C. ungeri (Karg, 1985) [described as H. (C.) ungeri, transferred to Cosmolaelaps by Farrier & Hennessey (1993)]; C. vacuus (Michael, 1891) [described as L. vacua, transferred to Cosmolaelaps by Hull (1918); senior synonym of H. militiformis Oudemans, 1902, according to Bregetova (1977); of Laeleps [sic] (C.) styliferus Halbert, 1915, according to Luxton (1998); of H. (C.) serratosimilis Bernhard in Hirschmann et al., 1969, according to Bregetova (1977)]; C. vanmoli (Loots, 1980) n. comb. [described as H. vanmoli]; C. wangae Bai & Gu, 1993; C. weeversi (Oudemans, 1926) [described as H. weeversi, transferred to Cosmolaelaps by Strandtmann (1963)]. For the characterisation of the following species, the reported references were used for complementary information, in addition to the original descriptions: Wang & Wang (1991) for C. acutiscutus; Evans & Till (1966) for C. claviger and C. cuneifer; Aswegen & Loots (1970) for C. digrediens; Schweizer (1961) for C. ornatus; Fouly et al. (1997) for C. simplex; Evans & Till (1966) for C. vacua and Strandtmann (1963) for C. weeversi. The following taxa may belong to Cosmolaelaps, but were not considered in the updated generic diagnosis because of the insufficient morphological information available: C. cuneifer var. americanus Berlese, 1904 [described as Laelaps (C.) cuneifer var. americanus], C. reconditus Berlese, 1905 [described as L. (C.) reconditus], C. vacuus var. ensiger Berlese, 1904 [described as L. (C.) vacuus var. ensiger], C. vacuus var. hastiger Berlese, 1920 and C. vacuus var. scalpiger (Berlese, 1904) [described as L. (C.) vacuus var. scalpiger].Published as part of Moreira, Grazielle Furtado, Klompen, Hans & Moraes, Gilberto José De, 2014, Redefinition of Cosmolaelaps Berlese (Acari: Laelapidae) and description of five new species from Brazil, pp. 317-346 in Zootaxa 3764 (3) on pages 318-320, DOI: 10.11646/zootaxa.3764.3.4, http://zenodo.org/record/23087