Bone marrow mononuclear cells autotransplant in experimental corneal ulcer in dogs

As células mononucleares (CM) da medula óssea (MO) despertam grande interesse nas pesquisas sobre regeneração tecidual. O limbo é a fonte de células-tronco (CT) para repor ceratócitos lesados e uma disfunção destas é denominada deficiência límbica. Essa condição é desenvolvida por diversas afecções, sendo que a queimadura por base é a mais comum. A fim de confirmar a presença das CM da MO transplantadas, a ocorrência de quimiotaxia destas e comparar histopatologicamente os grupos tratado e controle, utilizou-se um modelo experimental de úlcera de córnea associado ao autotransplante de CM. Para tanto, 16 cães machos ou fêmeas, sem raça definida, foram submetidos à úlcera experimental de córnea com papel filtro embebido em hidróxido de sódio (NaOH). Após as lesões, os animais foram submetidos a transplante subconjuntival de CM da MO, previamente marcadas com nanocristais. A avaliação pós-operatória foi realizada por imunofluorescência no sexto dia após o transplante e por histopatologia passados 15 dias do procedimento, quando foi possível notar que as CM fixaram-se na região lesionada, não sofreram quimiotaxia e, apesar de diminuírem a inflamação, não auxiliaram o processo de cicatrização corneana a curto prazo. Assim, sugerem-se estudos adicionais no transplante de CM da MO na cicatrização da córnea. _______________________________________________________________________________ ABSTRACTBone marrow (BM) mononuclear cells (MC) are a great subject in tissual regeneration. The main stem cell source to the eye is the limbus. Theses cells replace injured corneal cells, however, if the limbal stem cells are not functional, a limbal deficiency with concomitant conjunctivalization takes place. This pathological condition can be caused for several reasons, in which alkali burns are the most common. To conduct a research about transplanted BM MC presence, the cells homing and to histopathologically compare the treated and sham group, an experimental corneal ulcer model associated with MC autotransplant was used. Sixteen, male or female, stray dogs suffered experimental corneal ulceration with sodium hydroxide soaked filter discs. After the lesions, animals were submitted to subconjunctival autotransplant of previously marked BM MC. The evaluation was made by immunofluorescence on the sixth day after lesions creation and histopathology was conducted 15 days after the same procedure, when it was possible to observe that the MC grafted in the injured area, the cells did not execute the homing process and, despite the inflammatory decrease, they did not help the corneal epithelial healing process in a short term evaluation. Thus, future studies about MC transplantations in corneal ulcers are indicated

Radiographic study of the measures of the patella and the trochlear groove in toys healthy dogs and medial patellar luxation grades II and III carriers

Luxação medial da patela é uma afecção comum em cães de pequeno porte, devendo ser diagnosticada e tratada o mais cedo possível com objetivo de evitar maiores deformações e alterações articulares secundárias. O objetivo deste trabalho foi estudar as medidas e as relações anatômicas do sulco troclear e da patela através de exames radiográficos de cães sadios de raças predispostas à luxação patelar medial, analisando e comparando com o sulco troclear e patela de pacientes portadores desta enfermidade. Foram utilizados 40 joelhos de cães de raças predispostas que foram divididos em dois grupos, sendo o grupo 1 (G1) composto de 18 joelhos que não apresentavam luxação de patela e o grupo 2 (G2) composto de 22 joelhos que apresentem luxação de patela medial em graus II ou III. Foram realizadas mensurações de profundidade e largura do sulco troclear, altura e espessura da patela, percentual da patela dentro do sulco, ângulo do sulco troclear, sendo calculadas as razões entre largura patelar e do sulco troclear e profundidade do sulco troclear e espessura da patela. Todos os parâmetros estudados demonstraram diferença estatística significativa, na comparação entre cães sadios e portadores da afecção, ao serem avaliadas pelo teste t de Student. Através dessas medições realizadas nas radiografias dos cães do G1 foram desenvolvidas fórmulas preditivas do alargamento (Lsd = Lpp/0,89 – Lsp) e aprofundamento (Psd = 0,65Ep –Psp) do sulco troclear, para realização de trocleoplastia em bloco.Medial patellar dislocation is a common condition in small dogs and should be diagnosed and treated as early as possible in order to prevent further deformities and secondary osteoarthritis. The objective of this article was to study the measures and the anatomic relationships of the trochlear groove and patella in radiographic examinations of healthy dogs predisposed to medial patellar dislocation breeds, analyzing and comparing with the trochlear groove and patellar of patients with such disease. 40 knees of predisposed pure breeds dogs were used which were divided into two groups: group 1 (G1) consisting of 18 knees that do not show patellar dislocation and group 2 (G2) consisting of 22 knees showing patella in the medial grade II or III. Depth and width trochlear groove, height and thickness of the patella, a percentage of the patella in the groove, groove angle troclear were measured and calculated the ratios of patellar width and trochlear groove and depth of the trochlear groove and thickness of the patella. All parameters studied showed statistically significant differences, in comparison between healthy and affected dogs, compared to being evaluated by Student's t test. Through evaluation and measurements on radiographs G1 dogs, it were developed predictor formulas of broadening (Lsd = Lpp/0,89 – Lsp) and deepening (Psd = 0,65Ep –Psp) required the trochlear groove to perform trochleoplasty block

Colopexia em ovinos da raça Dorper com prolapso retal Colopexy in Dorper lambs with rectal prolapse

Prolapso de reto é afecção comum em ovinos de cauda curta. Neste trabalho relata-se a técnica de colopexia para redução de prolapso retal em trinta ovinos da raça Dorper, dos quais, três vieram a óbito no período pós-operatório e três tiveram que ser sacrificados, pois além de apresentarem recidiva, um deles era idoso, e os outros três por se encontrarem bastante debilitados. Aos 15 dias após a cirurgia, cinco animais apresentaram recidiva do prolapso, sendo a colopexia refeita em três deles tendo bom resultado em apenas um, e os outros dois foram sacrificados. Aos 30 dias de pós-operatório um animal apresentou prolapso retal, os outros dezenove (63,3%) estavam em adequado estado físico. A realização de colopexia é uma alternativa para o tratamento de prolapso retal em ovelhas da raça Dorper, porém recidivas e complicações são comuns.<br>Rectal prolapse is a common affection in lambs of short tail. This study aimed at reporting the colopexy to reduce the rectal prolapse in Dorper lambs. Thirty animals were submitted to surgery and three of them died in the postoperative period. Three animals had to be sacrificed, because they have shown prolapse recurrence (one of them was old, and the other three were in a strong debilitated state). At 15 days after the surgery, five animals showed prolapse recurrence and the colopexy was performed again in three having good results in only one, the other two were sacrificed. At the 30 postoperative days, an animal showed rectal prolapse signals, the other nineteen animals (63,3%) were healthy. The colopexy use is an option to rectal prolapse treatment in Dorper lambs, although, recurrences and complications are expected

Case study of canine brucellosis by brucella abortus: epidemiological inquiry

The objective of this research was to identify brucellosis by Brucella abortus and the possible risk factors associated with the transmission of the infection in dogs in the urban area of the municipality of Viçosa-AL, as well as to alert health authorities about the risk of this potential zoonosis, create a booklet to alert the population about the risks of the disease and the possibility of creating a test protocol in clinical care at the Veterinary Hospital of the Federal University of Alagoas. A study was carried out in the city of Viçosa-AL to investigate the presence of B. abortus in 30 adult male dogs. The animals that showed symptoms suggestive of reproductive disease were referred for evaluation of the hematological pattern and clinical and surgical treatment. Parallel to the blood collection, a questionnaire was applied to the owners of these dogs. Buffered acid antigen (AAT) techniques were used as a screening test and 2-Mercaptoethanol (2-ME) as a confirmatory test for AAT positives with symptoms of brucellosis. The study aimed to analyze the number of positive dogs, the clinical and anatomopathological changes and the risk factors for the occurrence of brucellosis in dogs in the municipality of ViçosaAL. As dogs are a means of transmission for humans, health authorities must pay special attention to this disease, including the control of dogs in the Program for Control and Eradication of Brucellosis of the Ministry of Agriculture. In addition to intensifying control of vaccination and diagnosis in cattle