Enumeration of RNA structures by Matrix Models

We enumerate the number of RNA contact structures according to their genus, i.e. the topological character of their pseudoknots. By using a recently proposed matrix model formulation for the RNA folding problem, we obtain exact results for the simple case of an RNA molecule with an infinitely flexible backbone, in which any arbitrary pair of bases is allowed. We analyze the distribution of the genus of pseudoknots as a function of the total number of nucleotides along the phosphate-sugar backbone.Comment: RevTeX, 4 pages, 2 figure

Legacy and shockwaves: A spatial analysis of strengthening resilience of the power grid in Connecticut

Grid resilience and reliability are pivotal in the transition to low and zero carbon energy systems. Tree-trimming operations (TTOs) have become a pivotal tool for increasing the resilience power grids, especially in highly forested regions. Building on recent literature, we aim at assessing the temporal and spatial extents of the benefits that TTOs produce on the grid from three perspectives: the frequency, extent, and duration of outages. We use a unique dataset provided by Eversource Energy, New England's largest utility company, with outage events from 2009 to 2015. We employ spatial econometrics to investigate both the legacy and spatial extent of TTOs. Our results show TTOs benefits occur for all three metrics for at least 4 years, and benefits spillover to up to 2 km throughout the treated areas, with significant spatial spillovers across the state greater than direct effects. Implications lead to supporting TTOs as part of the hardening policies for utility companies, especially as home-based activities increase in importance in a post-COVID19 world

Hyperbolic Balance Laws with a Non Local Source

This paper is devoted to hyperbolic systems of balance laws with non local source terms. The existence, uniqueness and Lipschitz dependence proved here comprise previous results in the literature and can be applied to physical models, such as Euler system for a radiating gas and Rosenau regularization of the Chapman-Enskog expansion.Comment: 26 page

Electrosynthesized poly(o-aminophenol) films as biomimetic coatings for dopamine detection on Pt substrates

Dopamine (DA) is a neurotransmitter, and its levels in the human body are associated with serious diseases. The need for a suitable detection method in medical practice has encouraged the development of electrochemical sensors that take advantage of DA electroactivity. Molecularly imprinted polymers (MIPs) are biomimetic materials able to selectively recognize target analytes. A novel MIP sensor for DA is proposed here based on a thin film of poly(o-aminophenol) electrosynthesized on bare Pt. A fast and easy method for executing the procedure for MIP deposition has been developed based on mild experimental conditions that are able to prevent electrode fouling from DA oxidation products. The MIP exhibited a limit of detection of 0.65 µM, and appreciable reproducibility and stability. The high recognition capability of poly(o-aminophenol) towards DA allowed for the achievement of notable selectivity: ascorbic acid, uric acid, serotonin, and tyramine did not interfere with DA detection, even at higher concentrations. The proposed sensor was successfully applied for DA detection in urine samples, showing good recovery

Fault Tolerance Analysis of a Bis-Ferrocene QCA Wire

Molecular Quantum Dot Cellular Automata (mQCA) are among the most promising emerging technologies for the expected theoretical operating frequencies (THz), the high device densities and the non-cryogenic working temperature. In this paper, we performed an analysis of the possible fabrication defects of a molecular QCA wire built with ad-hoc synthesized bis-ferrocene molecules. We then evaluated the fault tolerance of a real QCA device and assessed its performance in non-ideal conditions, by defining a new methodology for the fault analysis in the mQCA technology

Simulation of a molecular QCA wire

Molecular Quantum Dot Cellular Automata (MQCA) are among the most promising emerging technologies for the expected theoretical operating frequencies (THz), the high device densities and the non-cryogenic working temperature. In this work we simulated a molecular QCA wire, based on a molecule synthesized ad-hoc for this technology. The results discussed are obtained by means of iterative steps of ab-initio calculations

Management of vertebral osteomyelitis over an eight-year period: The UDIPROVE (UDIne PROtocol on VErtebral osteomyelitis)

Objectives: Vertebral osteomyelitis (VO) is a compelling clinical entity for clinicians because of its insidious and indolent course, which makes diagnosis difficult. Methods: All patients with a suspected diagnosis of VO were analyzed over an 8-year period (January 2009 to January 2017). The UDIPROVE protocol (UDIne PROtocol on VErtebral osteomyelitis) was applied in all cases. The primary endpoint was the performance of the UDIPROVE protocol to obtain the causal bacteria of infection. Results: During the study period, 133 episodes of confirmed VO were observed. The etiology of infection was obtained in 73.6% of cases: 70.5% were gram-positive, 16.3% were gram-negative, and 13.2% were mycobacteria. 18F-Fluorodeoxyglucose positron emission tomography/computed tomography (FDG-PET/CT) showed that for tubercular VO, the median standard uptake value (SUV) was higher when compared with VO caused by other bacteria. Clinical cure at the end of therapy was reported in 85.7% of patients. Previous antimicrobial therapy and a delay of more than 5 days in performing biopsy were associated with an undiagnosed etiology of VO. Targeted antibacterial therapy and follow-up with FDG-PET/CT were associated with clinical cure at the end of therapy, while the involvement of more than two vertebrae and inadequate drainage were associated with failure. Conclusions: Rigorous application of the UDIPROVE protocol allowed the causative pathogens of VO to be obtained \u2013 at about twice the rate reported in the literature. The use of FDG-PET/CT for the follow-up of infection was more reliable when compared to magnetic resonance imaging (MRI)

<b><i>Topoisomerase 1</i></b> Promoter Variants and Benefit from Irinotecan in Metastatic Colorectal Cancer Patients

Objective: Topoisomerase 1 (topo-1) is an important target for the treatment of metastatic colorectal cancer (CRC). The aim of the present study was to evaluate the correlation between topo-1 single-nucleotide polymorphisms (SNPs) and clinical outcome in metastatic CRC (mCRC) patients. Methods: With the use of specific software (PROMO 3.0), we performed an in silico analysis of topo-1 promoter SNPs; the rs6072249 and rs34282819 SNPs were included in the study. DNA was extracted from 105 mCRC patients treated with FOLFIRI ± bevacizumab in the first line. SNP genotyping was performed by real-time PCR. Genotypes were correlated with clinical parameters (objective response rate, progression-free survival, and overall survival). Results: No single genotype was significantly associated with clinical variables. The G allelic variant of rs6072249 topo-1 SNP is responsible for GC factor and X-box-binding protein transcription factor binding. The same allelic variant showed a nonsignificant trend toward a shorter progression-free survival (GG, 7.5 months; other genotypes, 9.3 months; HR 1.823, 95% CI 0.8904-3.734; p = 0.1). Conclusion: Further analyses are needed to confirm that the topo-1 SNP rs6072249 and transcription factor interaction could be a part of tools to predict clinical outcome in mCRC patients treated with irinotecan-based regimens