A Vision for Inclusion: An LGBT Broadband Future

This report provides an overview of the current scholarship and policy around the particular needs of LGBT people and the Internet

Hemolytic activity of adenylate cyclase toxin fromBordetella pertussis

AbstractAdenylate cyclase (AC) toxin fromB. pertussis enters eukaryotic cells where it produces supraphysiologie levels of cAMP. Purification of AC toxin activity [(1989) J. Biol. Chem. 264, 19279] results in increasing potency of hemolytic activity and electroelution of the 216-kDA holotoxin yields a single protein with AC enzymatic, toxin and hemolytic activities. AC toxin andE. coli hemolysin, which have DNA sequence homology [(1988) EMBO J. 7, 3997] are immunologically cross-reactive. The time courses of hemolysis elicited by the two molecules are strikingly different, however, with AC toxin eliciting cAMP accumulation with rapid onset, but hemolysis with a lag of ≥ 45 min. Finally, osmotic protection experiments indicate that the size of the putative pore produced by AC toxin is 3 5-fold smaller than that ofE. coli hemolysin

Accounting for Market Frictions and Power Asymmetries in Online Labor Markets

Amazon Mechanical Turk (AMT) is an online labor market that defines itself as "a marketplace for work that requires human intelligence." Early advocates and developers of crowdsourcing platforms argued that crowdsourcing tasks are designed so people of any skill level can do this labor online. However, as the popularity of crowdsourcing work has grown, the crowdsourcing literature has identified a peculiar issue: that work quality of workers is not responsive to changes in price. This means that unlike what economic theory would predict, paying crowdworkers higher wages does not lead to higher quality work. This has led some to believe that platforms, like AMT, attract poor quality workers. This article examines different market dynamics that might, unwittingly, contribute to the inefficiencies in the market that generate poor work quality. We argue that the cultural logics and socioeconomic values embedded in AMT's platform design generate a greater amount of market power for requesters (those posting tasks) than for individuals doing tasks for pay (crowdworkers). We attribute the uneven distribution of market power among participants to labor market frictions, primarily characterized by uncompetitive wage posting and incomplete information. Finally, recommendations are made for how to tackle these frictions when contemplating the design of an online labor market

Cattle Raiding, Cultural Survival, and Adaptability of East African Pastoralists

This is the published version. Also found here http://www.jstor.org/stable/359710

Mechanism of association of adenylate cyclase toxin with the surface of Bordetella pertussis : a role for toxin-filamentous haemagglutinin interaction

Summary Adenylate cyclase (AC) toxin from Bordetella pertussis is unusual in that, unlike most other members of the repeats-in-toxin family that are released into the extracellular milieu, it remains associated with the bacterial surface. In this study, we investigated the nature of the association of this toxin with the surface of B. pertussis . AC toxin was extracted from crude outer membrane preparations of B. pertussis with 8 M urea, but only partially with alkaline sodium carbonate and not at all with octylglucoside, suggesting that denaturation of the toxin is necessary for its removal from the membrane. B. pertussis mutants lacking filamentous haemagglutinin (FHA) released significantly more AC toxin into the medium, and AC toxin association with the bacterial surface was partially restored by expression of FHA from a plasmid, suggesting a role for FHA in surface retention of AC toxin. AC toxin distribution was unaffected by the absence of pertactin, or full-length lipopolysaccharide, or a defect in secretion of pertussis toxin. Using overlay and immunoprecipitation, we found that a direct physical association can occur between AC toxin and FHA. Combined, these findings suggest that FHA may play a role in AC toxin retention on the surface of B. pertussis and raise the possibility of an involvement of adherence mediated by FHA in delivery of AC toxin from the bacterium to the target cell

Standard survey methods for estimating colony losses and explanatory risk factors in Apis mellifera

This chapter addresses survey methodology and questionnaire design for the collection of data pertaining to estimation of honey bee colony loss rates and identification of risk factors for colony loss. Sources of error in surveys are described. Advantages and disadvantages of different random and non-random sampling strategies and different modes of data collection are presented to enable the researcher to make an informed choice. We discuss survey and questionnaire methodology in some detail, for the purpose of raising awareness of issues to be considered during the survey design stage in order to minimise error and bias in the results. Aspects of survey design are illustrated using surveys in Scotland. Part of a standardized questionnaire is given as a further example, developed by the COLOSS working group for Monitoring and Diagnosis. Approaches to data analysis are described, focussing on estimation of loss rates. Dutch monitoring data from 2012 were used for an example of a statistical analysis with the public domain R software. We demonstrate the estimation of the overall proportion of losses and corresponding confidence interval using a quasi-binomial model to account for extra-binomial variation. We also illustrate generalized linear model fitting when incorporating a single risk factor, and derivation of relevant confidence intervals

The Otterbein Miscellany - June 1982

https://digitalcommons.otterbein.edu/miscellany/1006/thumbnail.jp

Results of international standardised beekeeper surveys of colony losses for winter 2012-2013 : analysis of winter loss rates and mixed effects modelling of risk factors for winter loss.

This article presents results of an analysis of winter losses of honey bee colonies from 19 mainly European countries, most of which implemented the standardised 2013 COLOSS questionnaire. Generalised linear mixed effects models (GLMMs) were used to investigate the effects of several factors on the risk of colony loss, including different treatments for Varroa destructor, allowing for random effects of beekeeper and region. Both winter and summer treatments were considered, and the most common combinations of treatment and timing were used to define treatment factor levels. Overall and within country colony loss rates are presented. Significant factors in the model were found to be: percentage of young queens in the colonies before winter, extent of queen problems in summer, treatment of the varroa mite, and access by foraging honey bees to oilseed rape and maize. Spatial variation at the beekeeper level is shown across geographical regions using random effects from the fitted models, both before and after allowing for the effect of the significant terms in the model. This spatial variation is considerable

A novel whole-body thermal stress test for monitoring cardiovascular responses in guinea pigs

Cardiovascular disease is a leading cause of morbidity and mortality worldwide. Stress tests are frequently employed to expose early signs of cardiovascular dysfunction or disease and can be employed, for example, in the context of preterm birth. We aimed to establish a safe and effective thermal stress test to examine cardiovascular function. Guinea pigs were anaesthetized using a 0.8% isoflurane, 70% N2O mix. ECG, non-invasive blood pressure, laser Doppler flowmetry, respiratory rate, and an array of skin and rectal thermistors were applied. A physiologically relevant heating and a cooling thermal stress test was developed. Upper and lower thermal limits for core body temperature were set at 41.5 OC and 34 OC, for the safe recovery of animals. This protocol therefore presents a viable thermal stress test for use in guinea pig models of health and disease that facilitates exploration of whole-system cardiovascular function