Seeing Eye to I: Perceptual Development and Sense of Self

This paper is a commentary on current views of early development in art and argues for a theory which emphasizes a more active role of the learner in the refinement of perceptual ability, particularly in drawing development. Independent perception is presented as a primary source of children\u27s visual imagery: and inference and problem solving, rather than imitation, are seen to characterize the drawing process as well as to indicate proper methods of instruction. Discussion also considers the shift away from inquiry in this area

Hemolytic activity of adenylate cyclase toxin fromBordetella pertussis

AbstractAdenylate cyclase (AC) toxin fromB. pertussis enters eukaryotic cells where it produces supraphysiologie levels of cAMP. Purification of AC toxin activity [(1989) J. Biol. Chem. 264, 19279] results in increasing potency of hemolytic activity and electroelution of the 216-kDA holotoxin yields a single protein with AC enzymatic, toxin and hemolytic activities. AC toxin andE. coli hemolysin, which have DNA sequence homology [(1988) EMBO J. 7, 3997] are immunologically cross-reactive. The time courses of hemolysis elicited by the two molecules are strikingly different, however, with AC toxin eliciting cAMP accumulation with rapid onset, but hemolysis with a lag of ≥ 45 min. Finally, osmotic protection experiments indicate that the size of the putative pore produced by AC toxin is 3 5-fold smaller than that ofE. coli hemolysin

An interpretive analysis of elementary teachers' conceptions of caring

The purpose of this study was to examine the phenomenon of caring in the teacher-student relationship from the viewpoint of the teacher. This was accomplished in the following manner: a) constructing a conceptual framework of caring derived from the selected writings of major authors in this area; b) conducting a thematic analysis of in-depth interviews of teachers asked to reflect upon their conceptions of the caring teacher-student relationship; c) interpreting the emergent themes theoretically and personally; d) synthesizing the conceptual frameworks of the theoreticians and the practitioners into a comprehensive model of teacher caring

Mechanism of association of adenylate cyclase toxin with the surface of Bordetella pertussis : a role for toxin-filamentous haemagglutinin interaction

Summary Adenylate cyclase (AC) toxin from Bordetella pertussis is unusual in that, unlike most other members of the repeats-in-toxin family that are released into the extracellular milieu, it remains associated with the bacterial surface. In this study, we investigated the nature of the association of this toxin with the surface of B. pertussis . AC toxin was extracted from crude outer membrane preparations of B. pertussis with 8 M urea, but only partially with alkaline sodium carbonate and not at all with octylglucoside, suggesting that denaturation of the toxin is necessary for its removal from the membrane. B. pertussis mutants lacking filamentous haemagglutinin (FHA) released significantly more AC toxin into the medium, and AC toxin association with the bacterial surface was partially restored by expression of FHA from a plasmid, suggesting a role for FHA in surface retention of AC toxin. AC toxin distribution was unaffected by the absence of pertactin, or full-length lipopolysaccharide, or a defect in secretion of pertussis toxin. Using overlay and immunoprecipitation, we found that a direct physical association can occur between AC toxin and FHA. Combined, these findings suggest that FHA may play a role in AC toxin retention on the surface of B. pertussis and raise the possibility of an involvement of adherence mediated by FHA in delivery of AC toxin from the bacterium to the target cell

Transmission of Two Viruses that Cause Barley Yellow Dwarf is Controlled by Different Loci in the Aphid, Schizaphis graminum

Clonal populations of the aphid, Schizaphis graminum, have been separated into biotypes based on host preference and their ability to overcome resistance genes in wheat. Recently, several biotypes were found to differ in their ability to transmit one or more of the viruses that cause barley yellow dwarf disease in grain crops, and vector competence was linked to host preference. The genetics of host preference has been studied in S. graminum, but how this may relate to the transmission of plant viruses is unknown. Sexual morphs of a vector and nonvector S. graminum genotype were induced from parthenogenetic females and reciprocal crosses made. Eighty-nine hybrids were generated and maintained by parthenogenesis. Each hybrid was evaluated for its ability to transmit Barley yellow dwarf virus-PAV and Cereal yellow dwarf virus-RPV, and for its ability to colonize two wheat genotypes each expressing a different gene that confers resistance to S. graminum. The F1 genotypes were genetically variable for their ability to transmit virus and to colonize the aphid resistant wheat, but these traits were not genetically correlated. Individual F1 genotypes ranged in transmission efficiency from 0–100% for both viruses, although the overall mean transmission efficiency was similar to the transmission competent parent, indicating directional dominance. The direction of the cross did not significantly affect the vector competency for either virus, suggesting that maternally inherited cytoplasmic factors, or bacterial endosymbionts, did not contribute significantly to the inheritance of vector competency in S. graminum. Importantly, there was no genetic correlation between the ability to transmit Barley yellow dwarf virus and Cereal yellow dwarf virus-RPV in the F1 genotypes. These results taken together indicate that multiple loci are involved in the circulative transmission, and that the successful transmission of these closely related viruses is regulated by different sets of aphid genes

Early mortality among Aboriginal and Non-Aboriginal women who had a preterm birth in Western Australia: A population-based cohort study

Background: Having a preterm ( \u3c 37 weeks\u27 gestation) birth may increase a woman\u27s risk of early mortality. Aboriginal and Torres Strait Islander (hereafter Aboriginal) women have higher preterm birth and mortality rates compared with other Australian women. Objectives: We investigated whether a history of having a preterm birth was associated with early mortality in women and whether these associations differed by Aboriginal status. Methods: This retrospective cohort study used population-based perinatal records of women who had a singleton birth between 1980 and 2015 in Western Australia linked to Death Registry data until June 2018. The primary and secondary outcomes were all-cause and cause-specific mortality respectively. After stratification by Aboriginal status, rate differences were calculated, and Cox proportional hazard regression was used to estimate adjusted hazard ratios (HR) and 95 % confidence intervals (CI) for all-cause and cause-specific mortality. Results: There were 20,244 Aboriginal mothers (1349 deaths) and 457,357 non-Aboriginal mothers (7646 deaths) with 8.6 million person-years of follow-up. The all-cause mortality rates for Aboriginal mothers who had preterm births and term births were 529.5 and 344.0 (rate difference 185.5, 95 % CI 135.5, 238.5) per 100,000 person-years respectively. Among non-Aboriginal mothers, the corresponding figures were 125.5 and 88.6 (rate difference 37.0, 95 % CI 29.4, 44.9) per 100,000 person-years. The HR for all-cause mortality for Aboriginal and non-Aboriginal mothers associated with preterm birth were 1.48 (95 % CI 1.32, 1.66) and 1.35 (95 % CI 1.26, 1.44), respectively, compared with term birth. Compared with mothers who had term births, mothers of preterm births had higher relative risks of mortality from diabetes, cardiovascular, digestive and external causes. Conclusions: Both Aboriginal and non-Aboriginal women who had a preterm birth had a moderately increased risk of mortality up to 38 years after the birth, reinforcing the importance of primary prevention and ongoing screening

Relating agriculture, energy, and water decisions to farm incomes and climate projections using two freeware programs, FEWCalc and DSSAT

Context: The larger scale perspective of Integrated Assessment (IA) and smaller scale perspective of Impacts, Adaptation, and Vulnerability (IAV) need to be bridged to design long-term solutions to agricultural problems that threaten agricultural production, rural economic viability, and global food supplies. FEWCalc (Food-Energy-Water Calculator) is a new freeware, agent-based model with the novel ability to project farm incomes based on crop selection, irrigation practices, groundwater availability, renewable energy investment, and historical and projected environmental conditions. FEWCalc is used to analyze the interrelated food, energy, water, and climate systems of Finney County, Kansas to evaluate consequences of choices currently available to farmers and resource managers. Objective: This article aims to evaluate local farmer choices of crops and renewable energy investment in the face of water resource limitations and global climate change. Metrics of the analysis include agricultural and renewable-energy production, farm income, and water availability and quality. The intended audience includes farmers, resource managers, and scientists focusing on food, energy, and water systems. Methods: Data derived from publicly available sources are used to support user-specified FEWCalc input values. DSSAT (Decision Support System for Agrotechnology Transfer) with added arid-region dynamics is used to obtain simulated crop production and irrigation water demand for FEWCalc. Here, FEWCalc is used to simulate agricultural and energy production and farm income based on continuation of recent ranges of crop prices, farm expenses, and crop insurance; continuation of recent renewable-energy economics and government incentives; one of four climate scenarios, including General Circulation Model projections for Representative Concentration Pathway 8.5; and groundwater-supported irrigation and its limitations. Results and Conclusions: A 50-year (2018-2067) climate and groundwater availability projection process indicates possible trends of future crop yield, water utility, and farm income. The simulation during more wet years produces high crop production and slower depletion of groundwater, as expected. However, surprisingly, the simulations suggest that only the Drier Future scenario is commercially profitable, and this is because of reduced expenses for dryland farming. Although simulated income losses due to low crop production are ameliorated by the energy sector income and crop insurance, the simulation under climate change still produces the worst annual total income. Significance: FEWCalc addresses scientific, communication, and educational gaps between global- and local-scale FEW research communities and local stakeholders, affected by food, energy, water systems and their interactions by relating near-term choices to near- and long-term consequences. This analysis is needed to craft a more advantageous future

Avian Influenza among Waterfowl Hunters and Wildlife Professionals

We report serologic evidence of avian influenza infection in 1 duck hunter and 2 wildlife professionals with extensive histories of wild waterfowl and game bird exposure. Two laboratory methods showed evidence of past infection with influenza A/H11N9, a less common virus strain in wild ducks, in these 3 persons

Systems analysis of the transcriptional response of human ileocecal epithelial cells to Clostridium difficile toxins and effects on cell cycle control

<p>Abstract</p> <p>Background</p> <p>Toxins A and B (TcdA and TcdB) are <it>Clostridium difficile</it>'s principal virulence factors, yet the pathways by which they lead to inflammation and severe diarrhea remain unclear. Also, the relative role of either toxin during infection and the differences in their effects across cell lines is still poorly understood. To better understand their effects in a susceptible cell line, we analyzed the transciptome-wide gene expression response of human ileocecal epithelial cells (HCT-8) after 2, 6, and 24 hr of toxin exposure.</p> <p>Results</p> <p>We show that toxins elicit very similar changes in the gene expression of HCT-8 cells, with the TcdB response occurring sooner. The high similarity suggests differences between toxins are due to events beyond transcription of a single cell-type and that their relative potencies during infection may depend on differential effects across cell types within the intestine. We next performed an enrichment analysis to determine biological functions associated with changes in transcription. Differentially expressed genes were associated with response to external stimuli and apoptotic mechanisms and, at 24 hr, were predominately associated with cell-cycle control and DNA replication. To validate our systems approach, we subsequently verified a novel G₁/S and known G₂/M cell-cycle block and increased apoptosis as predicted from our enrichment analysis.</p> <p>Conclusions</p> <p>This study shows a successful example of a workflow deriving novel biological insight from transcriptome-wide gene expression. Importantly, we do not find any significant difference between TcdA and TcdB besides potency or kinetics. The role of each toxin in the inhibition of cell growth and proliferation, an important function of cells in the intestinal epithelium, is characterized.</p