Simultaneous evaluation of intact glucosinolates and phenolic compounds by UPLC-DAD-MS/MS in<em> Brassica oleracea</em> L. var.<em> botrytis</em>

International audienceA method for the simultaneous determination of intact glucosinolates and main phenolic compounds (flavonoids and sinapic acid derivatives) in Brassica oleracea L. var. botrytis was proposed. A simplified sample extraction procedure and a UPLC separation were carried out to reduce the total time of analysis. B. oleracea samples were added with internal standards (glucotropaeolin and rutin), and extracted with boiling methanol. crude extracts were evaporated under nitrogen, redissolved in mobile phase and analysed by UPLC with double detection (ESI(-)-MRM for glucosinolates and flavonoids, and DAD for main sinapic acid derivatives). The proposed method allowed a satisfactory quantification of main native sinapic acid derivatives, flavonoids and glucosinolates with a reduced time of analysis

Implicaciones de la reducción de los niveles de uso de nitratos y nitritos en la seguridad, conservación, características sensoriales y modificaciones tecnológicas de los productos cárnicos crudos-curados.

Capítulo V,15 páginas,14 figurasLa investigación que recoge este capítulo ha sido realizada en el marco del Proyecto Consolider-Ingenio 2010 CARNISENUSA CSD2007-00016.Peer reviewe