16,678 research outputs found
Diffusive Atomistic Dynamics of Edge Dislocations in Two Dimensions
The fundamental dislocation processes of glide, climb, and annihilation are
studied on diffusive time scales within the framework of a continuum field
theory, the Phase Field Crystals (PFC) model. Glide and climb are examined for
single edge dislocations subjected to shear and compressive strain,
respectively, in a two dimensional hexagonal lattice. It is shown that the
natural features of these processes are reproduced without any explicit
consideration of elasticity theory or ad hoc construction of microscopic
Peierls potentials. Particular attention is paid to the Peierls barrier for
dislocation glide/climb and the ensuing dynamic behavior as functions of strain
rate, temperature, and dislocation density. It is shown that the dynamics are
accurately described by simple viscous motion equations for an overdamped point
mass, where the dislocation mobility is the only adjustable parameter. The
critical distance for the annihilation of two edge dislocations as a function
of separation angle is also presented.Comment: 13 pages with 17 figures, submitted to Physical Review
Recommended from our members
Genome Editing Method for the Anaerobic Magnetotactic Bacterium Desulfovibrio magneticus RS-1.
Magnetosomes are complex bacterial organelles that serve as model systems for studying bacterial cell biology, biomineralization, and global iron cycling. Magnetosome biogenesis is primarily studied in two closely related Alphaproteobacteria of the genus Magnetospirillum that form cubooctahedral-shaped magnetite crystals within a lipid membrane. However, chemically and structurally distinct magnetic particles have been found in physiologically and phylogenetically diverse bacteria. Due to a lack of molecular genetic tools, the mechanistic diversity of magnetosome formation remains poorly understood. Desulfovibrio magneticus RS-1 is an anaerobic sulfate-reducing deltaproteobacterium that forms bullet-shaped magnetite crystals. A recent forward genetic screen identified 10 genes in the conserved magnetosome gene island of D. magneticus that are essential for its magnetic phenotype. However, this screen likely missed mutants with defects in crystal size, shape, and arrangement. Reverse genetics to target the remaining putative magnetosome genes using standard genetic methods of suicide vector integration have not been feasible due to the low transconjugation efficiency. Here, we present a reverse genetic method for targeted mutagenesis in D. magneticus using a replicative plasmid. To test this method, we generated a mutant resistant to 5-fluorouracil by making a markerless deletion of the upp gene that encodes uracil phosphoribosyltransferase. We also used this method for targeted marker exchange mutagenesis by replacing kupM, a gene identified in our previous screen as a magnetosome formation factor, with a streptomycin resistance cassette. Overall, our results show that targeted mutagenesis using a replicative plasmid is effective in D. magneticus and may also be applied to other genetically recalcitrant bacteria.IMPORTANCE Magnetotactic bacteria (MTB) are a group of organisms that form intracellular nanometer-scale magnetic crystals though a complex process involving lipid and protein scaffolds. These magnetic crystals and their lipid membranes, termed magnetosomes, are model systems for studying bacterial cell biology and biomineralization and are potential platforms for biotechnological applications. Due to a lack of genetic tools and unculturable representatives, the mechanisms of magnetosome formation in phylogenetically deeply branching MTB remain unknown. These MTB contain elongated bullet-/tooth-shaped magnetite and greigite crystals that likely form in a manner distinct from that of the cubooctahedral-shaped magnetite crystals of the genetically tractable MTB within the Alphaproteobacteria Here, we present a method for genome editing in Desulfovibrio magneticus RS-1, a cultured representative of the deeply branching MTB of the class Deltaproteobacteria This marks a crucial step in developing D. magneticus as a model for studying diverse mechanisms of magnetic particle formation by MTB
Development of a low-maintenance measurement approach to continuously estimate methane emissions: a case study
The chemical breakdown of organic matter in landfills represents a significant source of methane gas (CH4). Current estimates suggest that landfills are responsible for between 3% and 19% of global anthropogenic emissions. The net CH4 emissions resulting from biogeochemical processes and their modulation by microbes in landfills are poorly constrained by imprecise knowledge of environmental constraints. The uncertainty in absolute CH4 emissions from landfills is therefore considerable. This study investigates a new method to estimate the temporal variability of CH4 emissions using meteorological and CH4 concentration measurements downwind of a landfill site in Suffolk, UK from July to September 2014, taking advantage of the statistics that such a measurement approach offers versus shorter-term, but more complex and instantaneously accurate, flux snapshots. Methane emissions were calculated from CH4 concentrations measured 700 m from the perimeter of the landfill with observed concentrations ranging from background to 46.4 ppm. Using an atmospheric dispersion model, we estimate a mean emission flux of 709 μg m−2 s−1 over this period, with a maximum value of 6.21 mg m−2 s−1, reflecting the wide natural variability in biogeochemical and other environmental controls on net site emission. The emissions calculated suggest that meteorological conditions have an influence on the magnitude of CH4 emissions. We also investigate the factors responsible for the large variability observed in the estimated CH4 emissions, and suggest that the largest component arises from uncertainty in the spatial distribution of CH4 emissions within the landfill area. The results determined using the low-maintenance approach discussed in this paper suggest that a network of cheaper, less precise CH4 sensors could be used to measure a continuous CH4 emission time series from a landfill site, something that is not practical using far-field approaches such as tracer release methods. Even though there are limitations to the approach described here, this easy, low-maintenance, low-cost method could be used by landfill operators to estimate time-averaged CH4 emissions and their impact downwind by simultaneously monitoring plume advection and CH4 concentrations
SUMMARY OF CORROSION INVESTIGATIONS ON HIGH-TEMPERATURE ALUMINUM ALLOYS. Period covered : February 1955-October 1956
Tests were performed on aluminum alloys to evaluate their behavior in high-temperature, high-pressure. watercooled and -moderated nuclear reactor enviromnents. Test equipment, sample preparation. and test procedures are discussed. Aluminum nickel alloys were found resistant to disintegration for periods up to 60 days in dynamic water at 600 ction prod- F. The corrosion rates of the aluminum alloys M-388 and X-2219 at 600 ction prod- F were found to be too high to merit consideration for cladding materials. The influence of pH. gas content, and velocity of the water on the corrosion of the above alloys was evaluated. Hydrogen addition at startup appeared to increase the degree of corrosion attack on the M-388 alloy. Irradiation tests on aluminum-nickel alloys revealed that the corrosion rate increased with distance from core. In-reactor samples of M-388 exhibited less corrosion attack than out-of-reactor samples. Boiling water corrosion tests were performed on M-388 for 1612 hr at 422 ction prod- F with an average heat flux of 25,000 Btu per hrft/sup 2/. The over- all corrosion rate was 2.9 mil per yr. It is concluded that the corrosion rate of M-388 is acceptable for the specified test conditions: (1) absence of radiation: (2) demineralized water at 422 ction prod- F; and (3) heat transfer rates not exceeding 40,000 Btu per ft/sup 2/. No accelerated corrosion attack due to boiling heat transfer and no significant buildup of corrosion products on heat transfer surfaces were noted. Accelerated corrosion of M-388 was noted when coupled with Type 304 stainless steel and exposed to tap water at room temperature, or air-saturated, demineralized water at 680 ction prod- F. A technique for stripping corrosion films from aluminum and aluminum-nickel alloys is given. (C.J.G.
New Directions in Subband Coding
Two very different subband coders are described. The first is a modified dynamic bit-allocation-subband coder (D-SBC) designed for variable rate coding situations and easily adaptable to noisy channel environments. It can operate at rates as low as 12 kb/s and still give good quality speech. The second coder is a 16-kb/s waveform coder, based on a combination of subband coding and vector quantization (VQ-SBC). The key feature of this coder is its short coding delay, which makes it suitable for real-time communication networks. The speech quality of both coders has been enhanced by adaptive postfiltering. The coders have been implemented on a single AT&T DSP32 signal processo
Aerosol major ion record at Mount Washington
This study examined the seasonal cycles and regional-scale meteorological controls on the chemical properties of bulk aerosols collected from 1999 to 2004 at Mount Washington, the highest peak in the northeastern United States. The concentrations of NH4+ and SO42− peaked during summer months. The pattern for aerosol NO3− was more complicated with relatively high median concentrations characterizing spring and summer months, but with major elevated events occurring during fall, winter, and spring. The seasonal relationship between NH4+ and SO42− indicated that during warmer months a mixture of (NH4)2SO4 and NH4HSO4 was present, while it was mainly the latter in winter. More acidity and higher concentrations of the major species were generally associated with winds from the southwest and west sectors. The highest (≥95th percentile) concentrations of SO42− and NH4+ were associated with air mass transport from major upwind source regions in the Midwest and along the eastern seaboard. The ionic composition and seasonal cycle observed at Mount Washington were similar to those measured at other northeastern sites, but the range and average concentrations were much lower. These differences were exaggerated during wintertime. Included in this paper are several Eulerian case studies of SO2 conversion to SO42− during transit from Whiteface Mountain, New York, to Mount Washington. The calculations suggest a gas-phase SO2 oxidation rate of ∼1–2% per hour and demonstrate the possibility of using these two sites to investigate the chemical evolution of air masses as they move from Midwestern source regions to northern New England
- …