Zooplankton entrainment at the Surry Nuclear Power Plant, James River, Virginia : final report

The diverse zooplankton found in marine and estuarine waters serves an important role in food chains, converting phytoplankton and detrital material into protein rich animal tissues necessary in the nutrition of higher life forms, such as the young stages of decapod crustaceans and fishes. Generally free-floating or only weak swimmers, zooplankters are readily entrained in cooling waters pumped into power plants. Mortality of entrained organisms can be caused by mechanical abrasion, the length of time and amplitude of temperature increases during plant passage, and from chlorination of the system for fouling control. Estimates of the percent of mortality due to plant passage are difficult to obtain, however, because of the many variables that need to be taken into account. This project was designed to examine several of these variables as they apply to entrained zooplankton at the VEPCO Surry plant

Comparative strength of the 1966 year class of striped bass, Roccus saxatilis (Walbaum), in three Virginia rivers

The age composition, as determined from scale impressions, of striped bass stocks in the James, York, and Rappahannock Rivers during the period June 1967 - March 1968 indicates a relative deficiency of the 1966 year class in the James River. Similar results are shown in samples from non-selective gear (pound nets, fyke nets), selective gear (gill nets, haul seines, hook-and-line), and routine surveys using a 30-foot semi-balloon trawl.https://scholarworks.wm.edu/vimsbooks/1133/thumbnail.jp

Effects Of Tropical Storm Agnes On Zooplankton In The Lower Chesapeake Bay

Sampling techniques in use since August 1971 were employed to study effects of Tropical Storm Agnes on lower Chesapeake Bay zooplankton following the storm\u27s passage on June 21, 1972. Mean catches of copepods, cladocerans, barnacle larvae, decapod larvae, chaetognaths, and fish eggs and larvae were calculated for the entire study area and six subareas from 8 bongo net collections. A single subarea was selected for specific identifications within major taxa of zooplankton.https://scholarworks.wm.edu/vimsbooks/1072/thumbnail.jp

Preliminary Results from Striped Bass Tagging in Virginia, 1968-1969

A total of 8525 striped bass, Morone saxatilis (Walbaum), were tagged and released in Virginia during 1968 and 1969. Releases were grouped in three periods: (1) 3195 in winter 1968, (2) 2439 during summer-fall 1968; and (3) 2891 in winter 1969. Streamer disc tags, employed in winter 1968, were subsequently replaced by internal anchor tags (Floy Tag No. FD-67). This substitution shortened application time and eliminated a source of bias introduced by the entanglement of disc tags in gill nets. Releases were made in the James, York, and Rappahannock rivers in all three periods. Rewards of one dollar have been paid for return of tags. Percentages of returns within tagged year-classes increased with age, indicating change in fishing mortality rates of striped bass during their initial 3 to 4-year residence in the lower Chesapeake Bay system.https://scholarworks.wm.edu/vimsbooks/1106/thumbnail.jp

Efficiency and Power as a Function of Sequence Coverage, SNP Array Density, and Imputation

High coverage whole genome sequencing provides near complete information about genetic variation. However, other technologies can be more efficient in some settings by (a) reducing redundant coverage within samples and (b) exploiting patterns of genetic variation across samples. To characterize as many samples as possible, many genetic studies therefore employ lower coverage sequencing or SNP array genotyping coupled to statistical imputation. To compare these approaches individually and in conjunction, we developed a statistical framework to estimate genotypes jointly from sequence reads, array intensities, and imputation. In European samples, we find similar sensitivity (89%) and specificity (99.6%) from imputation with either 1× sequencing or 1 M SNP arrays. Sensitivity is increased, particularly for low-frequency polymorphisms (MAF <5%), when low coverage sequence reads are added to dense genome-wide SNP arrays — the converse, however, is not true. At sites where sequence reads and array intensities produce different sample genotypes, joint analysis reduces genotype errors and identifies novel error modes. Our joint framework informs the use of next-generation sequencing in genome wide association studies and supports development of improved methods for genotype calling

Bacterial swarming reduces Proteus mirabilis and Vibrio parahaemolyticus cell stiffness and increases β-lactam susceptibility

Swarmer cells of the gram-negative pathogenic bacteria Proteus mirabilis and Vibrio parahaemolyticus become long (>10-100 microns) and multinucleate during their growth and motility on polymer surfaces. We demonstrate increasing cell length is accompanied by a large increase in flexibility. Using a microfluidic assay to measure single-cell mechanics, we identified large differences in swarmer cell stiffness of (bending rigidity of P. mirabilis, 9.6 x 10^(-22) N m^2; V. parahaemolyticus, 9.7 x 10^(-23) N m^2) compared to vegetative cells (1.4 x 10^(-20) N m^2 and 3.2 x 10^(-22) N m^2, respectively). The reduction in bending rigidity (~3-15 fold) was accompanied by a decrease in the average polysaccharide strand length of the peptidoglycan layer of the cell wall from 28-30 to 19-22 disaccharides. Atomic force microscopy revealed a reduction in P. mirabilis peptidoglycan thickness from 1.5 nm (vegetative) to 1.0 nm (swarmer) and electron cryotomography indicated changes in swarmer cell wall morphology. P. mirabilis and V. parahaemolyticus swarmer cells became increasingly sensitive to osmotic pressure and susceptible to cell wall-modifying antibiotics (compared to vegetative cells)--they were ~30% more likely to die after 3 h of treatment with minimum inhibitory concentrations of the beta-lactams cephalexin and penicillin G. Long, flexible swarmer cells enables these pathogenic bacteria to form multicellular structures and promotes community motility. The adaptive cost of swarming is offset by a fitness cost in which cells are more susceptible to physical and chemical changes in their environment, thereby suggesting the development of new chemotherapies for bacteria that leverage swarming for survival

Application of Targeted Mass Spectrometry for the Quantification of Sirtuins in the Central Nervous System

Sirtuin proteins have a variety of intracellular targets, thereby regulating multiple biological pathways including neurodegeneration. However, relatively little is currently known about the role or expression of the 7 mammalian sirtuins in the central nervous system. Western blotting, PCR and ELISA are the main techniques currently used to measure sirtuin levels. To achieve sufficient sensitivity and selectivity in a multiplex-format, a targeted mass spectrometric assay was developed and validated for the quantification of all seven mammalian sirtuins (SIRT1-7). Quantification of all peptides was by multiple reaction monitoring (MRM) using three mass transitions per protein-specific peptide, two specific peptides for each sirtuin and a stable isotope labelled internal standard. The assay was applied to a variety of samples including cultured brain cells, mammalian brain tissue, CSF and plasma. All sirtuin peptides were detected in the human brain, with SIRT2 being the most abundant. Sirtuins were also detected in human CSF and plasma, and guinea pig and mouse tissues. In conclusion, we have successfully applied MRM mass spectrometry for the detection and quantification of sirtuin proteins in the central nervous system, paving the way for more quantitative and functional studies