Experimental investigation into localized instabilities of mixed Rayleigh-Bénard-Poiseuille convection

The stability of the Rayleigh-Bénard-Poiseuille flow in a channel with large transverse aspect ratio (ratio of width to vertical channel height) is studied experimentally. The onset of thermal convection in the form of ‘transverse rolls' (rolls with axes perpendicular to the Poiseuille flow direction) is determined in the Reynolds-Rayleigh number plane for two different working fluids: water and mineral oil with Prandtl numbers of approximately 6.5 and 450, respectively. By analysing experimental realizations of the system impulse response it is demonstrated that the observed onset of transverse rolls corresponds to their transition from convective to absolute instability. Finally, the system response to localized patches of supercriticality (in practice local ‘hot spots') is observed and compared with analytical and numerical results of Martinand, Carrière & Monkewitz (J. Fluid Mech., vol. 502, 2004, p. 175 and vol. 551, 2006, p. 275). The experimentally observed two-dimensional saturated global modes associated with these patches appear to be of the ‘steep' variety, analogous to the one-dimensional steep nonlinear modes of Pier, Huerre & Chomaz (Physica D, vol. 148, 2001, p. 49

Acute Vascular Effects of Aquatic and Land Treadmill Exercise

Download PD

Heart Rate Variability Responses to Exercise in Mid-Spectrum Chronic Kidney Disease

Heart rate variability (HRV) is a measure of autonomic nervous system (ANS) activity, and decreased HRV is associated with many cardiovascular conditions. Chronic kidney disease (CKD) is characterized by a decrease in renal function and may be associated with ANS imbalances in the renal vasculature. Low HRV is associated with CKD incidence. Exercise is able to alter HRV by modulating the ANS. The effect of exercise on HRV in mid-spectrum CKD patients remains understudied. PURPOSE: To determine the effect of steady-state exercise (SSE) and high-intensity interval exercise (HIIE) on post-exercise HRV in patients with stage 3 or 4 CKD. METHODS: Twenty participants with stage 3 or 4 CKD (n = 6 men; n = 14 women; age 62.0 ± 9.9 yr; weight 80.9 ± 16.2 kg; body fat 37.3 ± 8.5% of weight; VO2max 19.4 ± 4.7 ml/kg/min, eGFR 51.5 ± 6.82). On separate days, each participant completed 30 minutes of aerobic exercise on the treadmill with exercise intensities set at 65% VO2reserve for SSE and 90% and 20% of VO2reserve in 3:2 min ratio for HIIE in a randomized crossover design. Both conditions averaged ~ 65% VO2reserve. HRV was measured at baseline, immediately post-exercise (IPE), 1-hr post-exercise, and 24-hr post-exercise. HRV was measured for 5 mins in the supine position using an elastic belt and Bluetooth monitor (Polar H7). CardioMood software was used to process HRV variables high frequency (HF), low frequency (LF), and standard deviation of all NN intervals (SDNN). Data were analyzed using 2 (condition) by 4 (time) repeated-measures ANOVAs. Data violated normality and were natural log (ln) transformed prior to analysis. Significant main effects were followed up using pairwise comparisons using a Bonferroni adjustment for multiple comparisons. All analyses were performed using SPSS (v.26). RESULTS: For ln LF/HF there were no significant main effects for exercise condition, time, or their interaction (p \u3e 0.05). For ln HF (F = 3.507, p \u3c 0.05, ηp2 = 0.156), ln LF (F = 3.093, p \u3c 0.05, ηp2 = 0.140), and ln SDNN (F = 3.761, p \u3c 0.05, ηp2 = 0.165) there was a significant main effect for time. Post-hoc comparisons revealed that HF, LF, and SDNN were lower IPE than for all other time points. CONCLUSION: Thirty minutes of aerobic exercise transiently decreases HRV in mid-spectrum CKD patients. This response was not modified by exercise condition

Experimental investigation into localized instabilities of mixed Rayleigh-Benard-Poiseuille convection

The stability of the Rayleigh-Benard-Poiseuille flow in a channel with large transverse aspect ratio (ratio of width to vertical channel height) is studied experimentally. The onset of thermal convection in the form of 'transverse rolls' (rolls with axes perpendicular to the Poiscuille flow direction) is determined in the Reynolds-Rayleigh number plane for two different working fluids: water and mineral oil with Prandtl numbers of approximately 6.5 and 450, respectively. By analysing experimental realizations of the system Impulse response it is demonstrated that the observed onset of transverse rolls corresponds to their transition from convective to absolute instability. Finally, the system response to localized patches of supercriticality (in practice local 'hot spots') is observed and compared with analytical and numerical results of Martinand, Carriere & Monkewitz (J. Fluid Mech., vol. 502, 2004, p. 175 and vol. 551, 2006, p. 275). The experimentally observed two-dimensional saturated global modes associated with these patches appear to be of the 'steep' variety, analogous to the one-dimensional steep nonlinear modes of Pier, Huerre & Chomaz (Physica D, vol. 148, 2001, p. 49)

Impact of Acute Dietary Manipulations on Dual-Energy X-ray Absorptiometry Estimates of Visceral Adipose Tissue

Dual-energy x-ray absorptiometry (DXA) is viewed as a superior method of body composition assessment, but whole-body DXA scans are impacted by variation in pre-assessment activities, such as eating and drinking. DXA software now allows for estimation of visceral adipose tissue (VAT), which has been implicated in a number of diseases. It is unknown to what extent food and fluid intake affect VAT estimates. PURPOSE: determine the effects of acute high-carbohydrate (HC) and very low-carbohydrate (VLC) diets on DXA estimates of VAT. METHODS: Male and female adults completed two one-day dietary conditions in random order: a VLC diet (1 – 1.5 g CHO/kg) and a HC diet (9 g CHO/kg). The diets were isocaloric to each other, and all food items were provided to participants. DXA scans were conducted in the morning after an overnight fast and in the afternoon soon after the third standardized meal. VAT volume, mass, and area were obtained, and paired samples t-tests were performed to compare the changes in VAT measures between diets. RESULTS: Fifteen males (age 22 ± 3, BF% 21 ± 5%) and eighteen females (age 21 ± 2, BF% 31 ± 5%) were included in the analysis. The change in VAT volume between the fasted and fed visits was different between diets (HC: +1.6%; VLC: -9.2%, p= 0.047). There were also trends for differences in VAT mass (p= 0.089) and area (p= 0.096) changes between diets. CONCLUSIONS: Within a single day, VAT estimates are differentially affected by isocaloric HC and VLC diets, with VLC consumption leading to reductions in VAT estimates. The content of the diet on the day of a DXA scan can affect estimates of VAT, which could spuriously influence the categorization of an individual’s health risk by DXA VAT estimates. Standardization of food intake prior to scans, preferably in the form of an overnight fast, should be employed to eliminate this important source of error

Brachial Artery FMD and Endothelial Responses to High-Intensity Interval and Steady-State Moderate-Intensity Exercise

Brachial artery flow-mediated dilation (FMD) is a nitric oxide-dependent measure of conduit artery endothelial function that is potentiated by moderate- and high-intensity steady state exercise (SSE) for up to an hour after exercise; however, it is unclear whether high-intensity interval exercise (HIIE) provides a longer-lasting stimulus for enhancing FMD or greater oxidative and nitrative stress on the vascular endothelium than a comparable or greater amount of SSE. PURPOSE: Determine the influence of HIIE on post-exercise brachial artery FMD and the relationship between FMD and markers of endothelial function relative to a comparable amount of moderate-intensity SSE and a dose that is half that of SSE. METHODS: Seventeen male participants (age 27.8 + 6.4 yr; weight 80.6 + 9.0 kg; BMI 25.1 + 2.4 kg/m2; VO2max 52.1 + 7.5 ml/kg/min) underwent HIIE by treadmill running (90% and 40% of VO2reserve in 3:2 min ratio) to expend 500 kcals; HIIE to expend 250 kcals, and; SSE at 70% VO2reserve to expend 500 kcals in a randomized crossover design. All exercise conditions averaged 70% VO2reserve. Ultrasound measurements of brachial artery FMD and blood measures of total antioxidant capacity (TAC) in copper reducing equivalents, apolipoprotein A-1 (ApoA1: g/L), PON1 concentration (PON1c: mg/mL), arylesterase activity (PON1a: kU/L), soluble vascular adhesion molecule-1 (sVCAM-1: ng/mL) and nitrotyrosine (NT: nM) were obtained just before and 2 hr after exercise. FMD responses to exercise were analyzed using 3 (condition) by 2 (sample point) repeated measures ANOVAs. Pearson product-moment correlations of change variables (2 hr post-exercise – pre-exercise values) were calculated to determine relationships between FMD responses and blood variable responses to exercise. RESULTS: Brachial artery FMD responses were unaltered 2 hr after exercise in all three conditions (p \u3e 0.05). FMD responses were correlated with changes in PON1c (r = 0.221, p \u3c 0.0001) and inversely with changes TAC (r = -0.170, p \u3c 0.0001). Changes in s-VCAM1 were correlated with change in NT (r = 0.423, p \u3c 0.0001) and inversely with changes in PON1c (r = -0.177, p \u3c 0.0001). SUMMARY: Brachial artery FMD is unaltered 2 hr after HIIE or SSE of moderate duration in young fit men and does not appear to be related to responses in other markers of endothelial function

Quantification of In-Hospital Patient Mobilization after Cardiac Surgery using Accelerometers: There is More than Meets the Eye

Purpose Patients after cardiac surgery infrequently mobilize during their surgical ward stay. Patients are unaware why mobilization is important, and patients’ progress of mobilization activities is not available. The aim of this study was to use accelerometers with artificial intelligence algorithms for quantification of in-hospital mobilization after cardiac surgery. Methods Six static and dynamic patient activities were defined to measure patient mobilization. An accelerometer (AX3, Axivity) was postoperatively placed on both the upper arm and upper leg. An artificial neural network algorithm classified lying in bed, sitting in a chair, standing, walking, cycling on an exercise bike, and walking the stairs. The primary endpoint was each activity duration performed between 7 a.m. and 11 p.m. Secondary endpoints were length of intensive care unit stay and surgical ward stay. A subgroup analysis was performed for male and female patients. Results In total, 29 patients were classified after cardiac surgery with an intensive care unit stay of 1 (1–2) night and surgical ward stay of 5 (3–6) nights. Patients spent 41 (20–62) min less time in bed for each following hospital day (p<0.001). Standing (p=0.004), walking (p<0.001), and walking the stairs (p=0.001) increased during hospital stay. No differences between men (n=22) and women (n=7) were observed for all endpoints. Conclusion The approach presented in this study is applicable for measuring all six activities and for monitoring postoperative recovery of cardiac surgery patients. A next step is to provide tailored feedback to patients and healthcare professionals, to speed up recovery

Renal Function Responses to Steady-State Moderate-Intensity and High-Intensity Interval Exercise in Mid-Spectrum Chronic Kidney Disease

Efficacy of exercise to improve renal function remains understudied in adults with mid-spectrum chronic kidney disease (CKD). In addition, a comparison of steady-state exercise (SSE) and high-intensity interval exercise (HIIE) may contribute clinically-relevant information for exercise-related augmentation of renal function in mid-spectrum CKD. PURPOSE: To determine the influence of SSE and a comparable amount of HIIE on indicators of post-exercise renal function in patients diagnosed with secondary Stage 3 or 4 CKD. METHODS: Twenty participants (n = 6 men; n = 14 women; age 62.0 + 9.9 yr; weight 80.9 + 16.2 kg; body fat 37.3 + 8.5% of weight; VO2max 19.4 + 4.7 ml/kg/min) completed 30 min of SSE at 65% VO2reserve or HIIE by treadmill walking (90% and 20% of VO2reserve in 3:2 min ratio) in a randomized crossover design. Both exercise conditions averaged ~ 65% VO2reserve. Blood and urine samples were obtained by the same technician under standardized conditions just before, 1hr and 24hrs after exercise. Serum creatinine (sCR), urine epidermal growth factor ratio (uEGFr), cystatin C and estimates of glomerular filtration rate - modification of diet in renal disease (MDRD) and the CKD-EPI - responses were analyzed using 2 (condition) by 3 (sample point) repeated measures ANOVAs. RESULTS: sCR decreased from 1.45 + 0.05 pre-exercise to 1.26 + 0.05 mg/dl (-13%) 1hr after exercise and returned to pre-exercise levels by 24hr (p = 0.009). Both MDRD and CKD-EPI estimates of glomerular filtration rate were 16 to 19% higher at 1hr, returning to pre-exercise values by 24hrs after exercise. The MDRD estimate increased from 43.1 + 1.9 pre-exercise to 50.3 + 2.1 ml/min/1.73m2 1hr after exercise (p = 0.007) and CKD-EPI from 45.2 + 2.1 to 53.8 + 2.4 ml/min/1.73m2 at 1hr post-exercise (p = 0.009). Relative to pre-exercise measures, uEGFr remained stable with SSE but was 5.4% greater 24hr after HIIE (p = 0.052). Cystatin C remained stable in the hours after exercise (p \u3e 0.05). CONCLUSION: By clinical estimates, renal function was not normalized but transiently improved with SSE and HIIE in mid-spectrum CKD

Synthesis and Characterization of Two Metallic Spin-Glass Phases of FeMo₄Ge₃

Polycrystalline samples of FeMo4Ge3 have been synthesized by the reduction of an oxide mixture at 1248 K and characterized by a combination of diffraction, muon spin relaxation (µ+SR), Mössbauer spectroscopy, magnetometry, transport, and heat-capacity measurements. The compound adopts a tetragonal W5Si3 structure (space group I4/mcm); the iron and molybdenum atoms are disordered over two crystallographic sites, 16k and either 4a or 4b. The synthesis conditions determine which fourfold site is selected; occupation of either leads to the presence of one-dimensional chains of transition metals in the structure. In both cases, the electrical resistivity below 200 K is ~175 µΩ cm. The dc magnetization rapidly rises below 35 K (Fe/Mo on 16k and 4b sites) or 16 K (16k and 4a sites), and a magnetization of 1µB or 0.8µB per Fe atom is observed in 4 T at 2 K. The ac susceptibility and the heat capacity both suggest that these are glasslike magnetic transitions, although the transition shows a more complex temperature dependence (with two maxima in χ ) when the 4b sites are partially occupied by iron. No long-range magnetic order is thought to be present at 5 K in either structural form; this has been proven by neutron diffraction and µ+SR for the case when Fe and Mo occupy the 16k and 4b sites

High-Density Lipoprotein Antioxidant Responses to High-Intensity Interval and Steady-State Moderate-Intensity Exercise

High-intensity interval exercise (HIIE) may impart health benefits beyond what is acquired through moderate-intensity steady state exercise (SSE). Paraoxonase 1 (PON1), an antioxidant associated with high-density lipoprotein (HDL), may be altered with exercise; however, it is unclear whether HIIE provides a greater stimulus for increasing PON1 antioxidant activity than a comparable or greater amount of SSE. PURPOSE: Determine the influence of HIIE on PON1 concentration and activity relative to a comparable amount of moderate-intensity SSE and a dose that is half that of SSE. METHODS: Seventeen male participants (age 27.8 + 6.4 yr; weight 80.6 + 9.0 kg; BMI 25.1 + 2.4 kg/m2; %fat = 19 + 5; VO2max 52.1 + 7.5 ml/kg/min) underwent HIIE by treadmill running (90% and 40% of VO2reserve in 3:2 min ratio) to expend 500 kcals (H500); HIIE to expend 250 kcals (H250), and; SSE at 70% VO2reserve to expend 500 kcals (M500) in a randomized crossover design. Intensities of all exercise conditions averaged 70% VO2reserve. Blood measures of total antioxidant capacity (TAC) in copper reducing equivalents, HDL (g/mL), apolipoprotein A-1 (ApoA1: g/L), PON1 concentration (PON1c: g/mL) and arylesterase activity (PON1a: kU/L) were obtained just before, immediately after, 2 hr and 24 hr after exercise. Significant differences were determined using 3 by 4 repeated measures ANOVAs. Effect sizes were calculated to determine the magnitude of dependent variable responses to exercise. RESULTS: Pre-exercise HDL concentration was lower in H250 and increased most in H250 versus other exercise conditions (p \u3c 0.001, ES = 0.83). Other antioxidant responses were similar across exercise conditions. ApoA1 (+ 8.0%) and PON1a (+ 9.3%) increased immediately after exercise and remained elevated 24 hr after exercise (p \u3c 0.0001 for each; ApoA1 ES = 0.85, PON1a ES = 0.57). PON1c was increased 2.4% above baseline at 2 hr post-exercise (p = 0.0296, ES = 0.18) and TAC was elevated 8.6% above baseline at 24 hr post-exercise (p = 0.0227, ES = 0.48). SUMMARY: HDL and HDL antioxidant properties are transiently potentiated by HIIE with as little as 250 kcals of energy expenditure. HDL antioxidant activity and total antioxidant capacity are elevated with HIIE and SSE of moderate intensity in a similar manner and are observed for up to 24 hr after exercise