Use of timelapse photography to determine flower opening time and pattern in banana (Musa spp.) for efficient hand pollination

Open Access Journal; Published online: 30 Sep 2021Sterility and low seed set in bananas is the main challenge to their conventional genetic improvement. The first step to seed set in a banana breeding program depends on pollination at the right time to ensure effective fertilization. This study aimed at determining bract opening time (BOT) to enhance efficient pollination and seed set in bananas. A Nikon D810 digital camera was set-up to take pictures of growing banana inflorescences at five-minute intervals and time-lapse movies were developed at a speed of 30 frames per second to allow real-time monitoring of BOT. Genotypes studied included wild banana (1), Mchare (2), Matooke (4), Matooke hybrid (1), and plantain (1). Events of bract opening initiated by bract lift for female flowers (P < 0.01) started at 16:32 h and at 18:54 h for male flowers. Start of bract rolling was at 18:51 h among female flowers (P < 0.001) and 20:48 h for male flowers. Bracts ended rolling at 02:33 h and 01:16 h for female and flowers respectively (P < 0.05). Total time of bract opening (from lift to end of rolling) for female flowers was significantly longer than that of male flowers (P < 0.001). On average, the number of bracts subtending female flowers opening increased from one on the first day, to between one and four on the fourth day. The number regressed to one bract on day eight before start of opening of bracts subtending male flowers. There was a longer opening interval between bracts subtending female and male flowers constituting spatial and temporal separation. Bract rolling increased from partial to complete rolling from proximal to the distal end of the inflorescence among female flower. On the other hand, bracts subtending male flowers completely rolled. Differences in BOT of genotypes with the same reference time of assessment may be partly responsible for variable fertility. Hand pollination time between 07:00 and 10:00 h is slightly late thus an early feasible time should be tried

Bird species distributions across woodland canopy structure gradients

The tree canopy characteristics of two broadleaved woods in southern England were quantified in terms of two independent measures of structure, canopy height (calculated using heights ≥ 1 m) and percentage canopy cover (derived using heights 0.90, p < 0.001), there was a positive correlation across bird species between the mean values of canopy height and canopy cover associated with the mapped locations of each species. We suggest that canopy height acts as an effective surrogate of woodland structure and can be applied as a predictor of bird community composition and distribution, at least in lowland British conditions. Species associated with young growth had narrower niche breadths, as measured by differences in canopy height and canopy cover between the two woods, than did species associated with taller canopies. Remote sensing of canopy height potentially offers a simple, effective way of assessing habitat availability for many species, at both woodland and landscape scales. This may be especially relevant for species dependent on highly transient vegetation structures associated with the early pre-canopy closure stages of forest growth

The CEBAF large acceptance spectrometer (CLAS)

The CEBAF large acceptance spectrometer (CLAS) is used to study photo- and electro-induced nuclear and hadronic reactions by providing efficient detection of neutral and charged particles over a good fraction of the full solid angle. A collaboration of about 30 institutions has designed, assembled, and commissioned CLAS in Hall B at the Thomas Jefferson National Accelerator Facility. The CLAS detector is based on a novel six-coil toroidal magnet which provides a largely azimuthal field distribution. Trajectory reconstruction using drift chambers results in a momentum resolution of 0.5% at forward angles. Cherenkov counters, time-of-flight scintillators, and electromagnetic calorimeters provide good particle identification. Fast triggering and high data-acquisition rates allow operation at a luminosity of View the MathML source. These capabilities are being used in a broad experimental program to study the structure and interactions of mesons, nucleons, and nuclei using polarized and unpolarized electron and photon beams and targets. This paper is a comprehensive and general description of the design, construction and performance of CLAS