INFLUENCE OF DIETARY PATTERN AND METHYLENTETRAHYDROFOLATE REDUCTASE C677T POLYMORPHISM ON THE PLASMA HOMOCYSTEINE LEVEL AMONG HEALTHY VEGETARIANS AND OMNIVORES

Povišena razina homocisteina u plazmi (Hcy) povezana je s fiziološkim i prehrambenim čimbenicima, kao i genetskim defektom enzima koji su uključeni u metabolizam Hcy. Ciljevi ove studije bili su (1) utvrditi razlike između zdravih vegetarijanca i omnivora u odnosu na biokemijske parametare, prevalenciju MTHFR genotipa i razinu Hcy, i (2) utvrditi učinke polimorfizma metilentetrahidrofolat reduktaze (MTHFR) C677T i načina prehrane na razinu Hcy u plazmi. U 47 vegetarijanaca i 53 omnivora izmjerena je razina Hcy, folata, vitamina B12, glukoze, ukupnog kolesterola, triglicerida, HDL i LDL kolesterola i kreatinina u plazmi. Polimorfizam MTHFR C677T analiziran je pomoću PCR-RFLP metode. Dobiveni rezultati su pokazali da su vegetarijanci imali niži vitamin B12, ukupni kolesterol, LDL-kolesterol i status kreatinina. Razina Hcy u plazmi bila je veća kod vegetarijanaca u usporedbi s omnivorima (14,10 ± 6,69 vs 10,49 ± 2,41 μmol/L) i negativno je korelirala sa statusom vitamina B12 i folatom. Razina plazme Hcy nije bila različita u odnosu na genotipove MTHFR C677T, ni među vegetarijancima ni omnivorima. Za razliku od MTHFR C677T polimorfizma, potvrđen je utjecaj načina prehrane na razinu Hcy u plazmi. Može se zaključiti da vegetarijanci obično imaju niži status vitamina B12 i višu razinu Hcy u plazmi. Polimorfizam MTHFR C677T nema utjecaja na razinu Hcy plazme, za razliku od prehrambenog uzorka koji ukazuje na važnost adekvatnog vitamina B12 i statusa folata u zaobilaženju mutacije.Elevated total plasma homocysteine level (Hcy) is associated with physiological and dietary factors as well as the genetic defect of enzymes involved in Hcy metabolism. The objectives of the study were to examine (1) differences between healthy vegetarian and omnivorous subjects in relation to biochemical parameters, prevalence of the MTHFR (methylentetrahydrofolate reductase) T/T genotype, and the plasma Hcy level, and (2) the effects of the MTHFR C677T polymorphism and dietary pattern on the plasma Hcy level. In 47 vegetarian and 53 omnivorous subjects the plasma level of Hcy, folate, vitamin B12, glucose, total cholesterol, triglycerides, HDL and LDL-cholesterol and creatinine were measured. MTHFR C677T polymorphisms were analyzed using the PCR-RFLP method. Obtained results have shown that vegetarians had lower vitamin B12, total cholesterol, LDLcholesterol and creatinine status. The plasma Hcy level was higher among vegetarians compared with omnivore subjects (14.10±6.69 vs. 10.49±2.41 μmol/L) and negatively correlated with vitamin B12 status and folate. The plasma Hcy level did not differ between the given MTHFR C677T genotypes among either vegetarians or omnivores. Unlike the MTHFR C677T polymorphism, the effect of dietary pattern on plasma Hcy level was confirmed. It could be concluded that vegetarians tend to have lower vitamin B12 status and a higher plasma Hcy level. The MTHFR 677C/T polymorphism has no effect on plasma Hcy level, in contrast to dietary pattern which indicates the importance of adequate vitamin B12 and folate status in bypassing the mutation

INFLUENCE OF DIETARY PATTERN AND METHYLENTETRAHYDROFOLATE REDUCTASE C677T POLYMORPHISM ON THE PLASMA HOMOCYSTEINE LEVEL AMONG HEALTHY VEGETARIANS AND OMNIVORES

Povišena razina homocisteina u plazmi (Hcy) povezana je s fiziološkim i prehrambenim čimbenicima, kao i genetskim defektom enzima koji su uključeni u metabolizam Hcy. Ciljevi ove studije bili su (1) utvrditi razlike između zdravih vegetarijanca i omnivora u odnosu na biokemijske parametare, prevalenciju MTHFR genotipa i razinu Hcy, i (2) utvrditi učinke polimorfizma metilentetrahidrofolat reduktaze (MTHFR) C677T i načina prehrane na razinu Hcy u plazmi. U 47 vegetarijanaca i 53 omnivora izmjerena je razina Hcy, folata, vitamina B12, glukoze, ukupnog kolesterola, triglicerida, HDL i LDL kolesterola i kreatinina u plazmi. Polimorfizam MTHFR C677T analiziran je pomoću PCR-RFLP metode. Dobiveni rezultati su pokazali da su vegetarijanci imali niži vitamin B12, ukupni kolesterol, LDL-kolesterol i status kreatinina. Razina Hcy u plazmi bila je veća kod vegetarijanaca u usporedbi s omnivorima (14,10 ± 6,69 vs 10,49 ± 2,41 μmol/L) i negativno je korelirala sa statusom vitamina B12 i folatom. Razina plazme Hcy nije bila različita u odnosu na genotipove MTHFR C677T, ni među vegetarijancima ni omnivorima. Za razliku od MTHFR C677T polimorfizma, potvrđen je utjecaj načina prehrane na razinu Hcy u plazmi. Može se zaključiti da vegetarijanci obično imaju niži status vitamina B12 i višu razinu Hcy u plazmi. Polimorfizam MTHFR C677T nema utjecaja na razinu Hcy plazme, za razliku od prehrambenog uzorka koji ukazuje na važnost adekvatnog vitamina B12 i statusa folata u zaobilaženju mutacije.Elevated total plasma homocysteine level (Hcy) is associated with physiological and dietary factors as well as the genetic defect of enzymes involved in Hcy metabolism. The objectives of the study were to examine (1) differences between healthy vegetarian and omnivorous subjects in relation to biochemical parameters, prevalence of the MTHFR (methylentetrahydrofolate reductase) T/T genotype, and the plasma Hcy level, and (2) the effects of the MTHFR C677T polymorphism and dietary pattern on the plasma Hcy level. In 47 vegetarian and 53 omnivorous subjects the plasma level of Hcy, folate, vitamin B12, glucose, total cholesterol, triglycerides, HDL and LDL-cholesterol and creatinine were measured. MTHFR C677T polymorphisms were analyzed using the PCR-RFLP method. Obtained results have shown that vegetarians had lower vitamin B12, total cholesterol, LDLcholesterol and creatinine status. The plasma Hcy level was higher among vegetarians compared with omnivore subjects (14.10±6.69 vs. 10.49±2.41 μmol/L) and negatively correlated with vitamin B12 status and folate. The plasma Hcy level did not differ between the given MTHFR C677T genotypes among either vegetarians or omnivores. Unlike the MTHFR C677T polymorphism, the effect of dietary pattern on plasma Hcy level was confirmed. It could be concluded that vegetarians tend to have lower vitamin B12 status and a higher plasma Hcy level. The MTHFR 677C/T polymorphism has no effect on plasma Hcy level, in contrast to dietary pattern which indicates the importance of adequate vitamin B12 and folate status in bypassing the mutation

INFLUENCE OF DIETARY PATTERN AND METHYLENTETRAHYDROFOLATE REDUCTASE C677T POLYMORPHISM ON THE PLASMA HOMOCYSTEINE LEVEL AMONG HEALTHY VEGETARIANS AND OMNIVORES

Povišena razina homocisteina u plazmi (Hcy) povezana je s fiziološkim i prehrambenim čimbenicima, kao i genetskim defektom enzima koji su uključeni u metabolizam Hcy. Ciljevi ove studije bili su (1) utvrditi razlike između zdravih vegetarijanca i omnivora u odnosu na biokemijske parametare, prevalenciju MTHFR genotipa i razinu Hcy, i (2) utvrditi učinke polimorfizma metilentetrahidrofolat reduktaze (MTHFR) C677T i načina prehrane na razinu Hcy u plazmi. U 47 vegetarijanaca i 53 omnivora izmjerena je razina Hcy, folata, vitamina B12, glukoze, ukupnog kolesterola, triglicerida, HDL i LDL kolesterola i kreatinina u plazmi. Polimorfizam MTHFR C677T analiziran je pomoću PCR-RFLP metode. Dobiveni rezultati su pokazali da su vegetarijanci imali niži vitamin B12, ukupni kolesterol, LDL-kolesterol i status kreatinina. Razina Hcy u plazmi bila je veća kod vegetarijanaca u usporedbi s omnivorima (14,10 ± 6,69 vs 10,49 ± 2,41 μmol/L) i negativno je korelirala sa statusom vitamina B12 i folatom. Razina plazme Hcy nije bila različita u odnosu na genotipove MTHFR C677T, ni među vegetarijancima ni omnivorima. Za razliku od MTHFR C677T polimorfizma, potvrđen je utjecaj načina prehrane na razinu Hcy u plazmi. Može se zaključiti da vegetarijanci obično imaju niži status vitamina B12 i višu razinu Hcy u plazmi. Polimorfizam MTHFR C677T nema utjecaja na razinu Hcy plazme, za razliku od prehrambenog uzorka koji ukazuje na važnost adekvatnog vitamina B12 i statusa folata u zaobilaženju mutacije.Elevated total plasma homocysteine level (Hcy) is associated with physiological and dietary factors as well as the genetic defect of enzymes involved in Hcy metabolism. The objectives of the study were to examine (1) differences between healthy vegetarian and omnivorous subjects in relation to biochemical parameters, prevalence of the MTHFR (methylentetrahydrofolate reductase) T/T genotype, and the plasma Hcy level, and (2) the effects of the MTHFR C677T polymorphism and dietary pattern on the plasma Hcy level. In 47 vegetarian and 53 omnivorous subjects the plasma level of Hcy, folate, vitamin B12, glucose, total cholesterol, triglycerides, HDL and LDL-cholesterol and creatinine were measured. MTHFR C677T polymorphisms were analyzed using the PCR-RFLP method. Obtained results have shown that vegetarians had lower vitamin B12, total cholesterol, LDLcholesterol and creatinine status. The plasma Hcy level was higher among vegetarians compared with omnivore subjects (14.10±6.69 vs. 10.49±2.41 μmol/L) and negatively correlated with vitamin B12 status and folate. The plasma Hcy level did not differ between the given MTHFR C677T genotypes among either vegetarians or omnivores. Unlike the MTHFR C677T polymorphism, the effect of dietary pattern on plasma Hcy level was confirmed. It could be concluded that vegetarians tend to have lower vitamin B12 status and a higher plasma Hcy level. The MTHFR 677C/T polymorphism has no effect on plasma Hcy level, in contrast to dietary pattern which indicates the importance of adequate vitamin B12 and folate status in bypassing the mutation

Importance of Selection a Method of Reconstruction of Digestive Continuity After Gastrectomy

Introduction: Gastrectomy is one of the most common surgical methods for the treatment of gastric cancer, which basically destroys the mechanism and digestion chemistry. Reconstruction after gastrectomy attempts to optimize the antireflux and nutritive component of the postgastrectomic syndrome.Objective: To determine which reconstructive method after gastrectomy has the optimal synthesis of antireflux and nutritional components.Patients and Methods: 111 patients were treated for gastric malignancies at the Surgical Clinic of the University Clinical Center in Banja Luka, which were operated with the intention of achieving curability.Results: Based on Fisher’s exact probability test there is no statistically significant difference (p> 0.05) in mortality compared to the restoration of digestive continuity after gastrectomy. Reflux oesophagitis is the dominant modality of morbidity in omega-loop reconstruction (p <0.05). There is no statistically significant difference (p> 0.05) in late dumping syndrome in patients relative to individual gastric substitution options. In the Hunt-Lawrence-Rodino pouch reconstruction option, there is no statistically significant difference (p> 0.05) in the participation of individual modalities of meal quantity in relation to the condition before the disease or the modality of the nutritional status. .Conclusion: The results indicate the antireflux component of reconstruction Roux en Y and the advantage of the nutritive component in the loop modification (the creation of the Hunt-Lawrence-Rodino pouch)