A metagenome for lacustrine Cladophora (Cladophorales) reveals remarkable diversity of eukaryotic epibionts and genes relevant to materials cycling

Periphyton dominated by the cellulose-rich filamentous green alga Cladophora forms conspicuous growths along rocky marine and freshwater shorelines worldwide, providing habitat for diverse epibionts. Bacterial epibionts have been inferred to display diverse functions of biogeochemical significance: N-fixation and other redox reactions, phosphorus accumulation, and organic degradation. Here, we report taxonomic diversity of eukaryotic and prokaryotic epibionts and diversity of genes associated with materials cycling in a Cladophora metagenome sampled from Lake Mendota, Dane Co., WI, USA, during the growing season of 2012. A total of 1,060 distinct 16S, 173 18S, and 351 28S rRNA operational taxonomic units, from which >220 genera or species of bacteria (~60), protists (~80), fungi (6), and microscopic metazoa (~80), were distinguished with the use of reference databases. We inferred the presence of several algal taxa generally associated with marine systems and detected Jaoa, a freshwater periphytic ulvophyte previously thought endemic to China. We identified six distinct nifH gene sequences marking nitrogen fixation, >25 bacterial and eukaryotic cellulases relevant to sedimentary C-cycling and technological applications, and genes encoding enzymes in aerobic and anaerobic pathways for vitamin B12 biosynthesis. These results emphasize the importance of Cladophora in providing habitat for microscopic metazoa, fungi, protists, and bacteria that are often inconspicuous, yet play important roles in ecosystem biogeochemistry

Autonomic physiological data associated with simulator discomfort

The development of a physiological monitoring capability for the Army's advanced helicopter simulator facility is reported. Additionally, preliminary physiological data is presented. Our objective was to demonstrate the sensitivity of physiological measures in this simulator to self-reported simulator sickness. The data suggested that heart period, hypergastria, and skin conductance level were more sensitive to simulator sickness than were vagal tone and normal electrogastric activity

Novel audio lures to improve interaction and encounter rates of possums (Trichosurus vulpecula) with control methods in New Zealand

The brushtail possum (Trichosurus vulpecula), a marsupial native to Australia, was widely introduced in both the North and South Islands of New Zealand between 1890 and 1930 to support the fur industry that was booming in Australia at the time. Possums were one of the many mammalian species that humans introduced when they discovered and settled in New Zealand. Before the settlement of humans in New Zealand, there were no terrestrial mammals; therefore, the local species evolved in the absence of mammalian predators. This resulted in native species populations falling at alarming rates and possibly facing extinction due to numerous predators and species that had not been intended to coexist alongside one another. The government's initiative to reduce New Zealand's predators (possums, rats, and stoats) by 2050 has significantly expanded efforts to eliminate these pest mammals in recent years. Managing these pest mammal populations is crucial to protect the native bird, reptile, and invertebrate species. In New Zealand, predator control has been practiced for a long time, but what started with simple trapping has since developed into a thriving industry full of expertise to boost the efficiency of trapping and toxins, including species-specific attractants. The number of traps and bait stations needed to be set up and the associated labour and expense would be significantly reduced if animals could be reliably drawn from a distance to a bait station or trap. Any sound played to encourage or discourage interaction with a control tool/area is known as an audio lure. This research aims to identify if audio lures can significantly increase possum encounter and interaction rates of control devices and establish an audio lure tool that is durable, easy to use, and cost-effective for the wider public to use. Preliminary captive trials found that an aggressive possum sound is significantly more attractive to possums than an alarmed possum sound, a beeping sound, or a control (no sound). Preliminary field trials have found that the audio lure being developed performs similarly to an already established audio lure included as part of the Cacophony Project Thermal Camera. The price point of the audio lure being developed is around $250 (NZD) and should last 30 days in the field. At the conference, I will present the final captive trial results and more field trial research. The field trial research will investigate encounter and interaction rates of possums feeding in live-capture traps with and without audio lures. I will also, report the final audio lure costings and field life before servicing is required

Hot Stars and Cool Clouds: The Photodissociation Region M16

We present high-resolution spectroscopy and images of a photodissociation region (PDR) in M16 obtained during commissioning of NIRSPEC on the Keck II telescope. PDRs play a significant role in regulating star formation, and M16 offers the opportunity to examine the physical processes of a PDR in detail. We simultaneously observe both the molecular and ionized phases of the PDR and resolve the spatial and kinematic differences between them. The most prominent regions of the PDR are viewed edge-on. Fluorescent emission from nearby stars is the primary excitation source, although collisions also preferentially populate the lowest vibrational levels of H2. Variations in density-sensitive emission line ratios demonstrate that the molecular cloud is clumpy, with an average density n = 3x10^5 cm^(-3). We measure the kinetic temperature of the molecular region directly and find T_H2 = 930 K. The observed density, temperature, and UV flux imply a photoelectric heating efficiency of 4%. In the ionized region, n_i=5x10^3 cm^(-3) and T_HII = 9500 K. In the brightest regions of the PDR, the recombination line widths include a non-thermal component, which we attribute to viewing geometry.Comment: 5 pages including 2 Postscript figures. To appear in ApJ Letters, April 200

Discovery of an extended debris disk around the F2V star HD 15745

Using the Advanced Camera for Surveys aboard the Hubble Space Telescope, we have discovered dust-scattered light from the debris disk surrounding the F2V star HD 15745. The circumstellar disk is detected between 2.0" and 7.5" radius, corresponding to 128 - 480 AU radius. The circumstellar disk morphology is asymmetric about the star, resembling a fan, and consistent with forward scattering grains in an optically thin disk with an inclination of ~67 degrees to our line of sight. The spectral energy distribution and scattered light morphology can be approximated with a model disk composed of silicate grains between 60 and 450 AU radius, with a total dust mass of 10E-7 M_sun (0.03 M_earth) representing a narrow grain size distribution (1 - 10 micron). Galactic space motions are similar to the Castor Moving Group with an age of ~10E+8 yr, although future work is required to determine the age of HD 15745 using other indicators.Comment: 7 pages, 4 figures, ApJ Letters, in pres