4 research outputs found

    Hémoparasites Chez Les Ruminants Domestiques Dans Les Départements De Korhogo Et Sinématiali En Côte d’Ivoire

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    L’étude réalisée de janvier à décembre 2019 dans le Nord de la Côte d’Ivoire a pour but de déterminer la prévalence des hémoparasites chez les bovins et ovins d’élevage. Des frottis sanguins ont été réalisés à partir du sang périphérique de l’oreille de 68 bovins et 64 ovins provenant des localités de Korhogo, Nafoun, Nagougaha, Mongaha et Lokoli. Les résultats obtenus de l’étude proviennent du registre de laboratoire collectés sur l’année 2019. L’observation microscopique des frottis sanguins a permis d’identifier trois genres d’hémoparasites : Anaplasma, Babesia et Theileria. Theileria est absent chez les ovins. Concernant l’espèce d’hémoparasite, les ovins (71,88%) et les bovins (70,59%) sont plus infectés par Anaplasma marginale avec une différence significative (p-value = 0,0001). Par ailleurs, les bovins de plus d’un an sont plus infectés par Anaplasma marginale. La prévalence d’infection est de 32,35% pour ceux d’un an à 3ans et 29,41% pour ceux de plus de 3ans d’âge. Les femelles (54,41%) sont les plus infectées. Les ovins de plus de 3 mois d’âge sont plus infectés. En outre, les femelles (45,31%) sont les plus infectées avec une différence significative (p-value = 0,0261) pour Anaplasma marginale. Les bovins de la localité de Korhogo sont plus infectés par Anaplasma marginale (36,76%) suivis des bovins de Nagougaha (20,59%) avec une différence significative (p-value = 0,0077). Par ailleurs, Anaplasma centrale (18,75%) infecte plus les ovins de la localité de Lokoli suivi de Korhogo (4,69%) avec une différence significative (p-value = 0,00037). The study carried out from January to December 2019 in the North of Côte d'Ivoire aims to determine the prevalence of hemoparasites in cattle and sheep breeding. Blood smears were taken from the peripheral ears of 68 cattle and 64 sheep from the localities of Korhogo, Nafoun, Nagougaha, Mongaha and Lokoli. The results obtained from the study come from the laboratory register collected in 2019. Microscopic examination of blood smears has identified three types of hemoparasites: Anaplasma, Babesia and Theileria. Theileria is absent in sheep. At the level of hemoparasite species, sheep (71.88%) and cattle (70.59%) are more infected by Anaplasma marginale with a significant difference (p-value = 0.0001). In addition, cattle over one year are more infected with Anaplasma marginale. The prevalence of infection is 32.35% for one year to 3 years and 29.41% for over 3 years of age. Females (54.41%) are the most infected. Sheep over 3 months of age are more infected. Females (45.31%) are the most infected with a significant difference (p-value = 0.0261) for Anaplasma marginale. Cattle of Korhogo are more infected with Anaplasma marginale (36.76%) followed by Nagougaha (20.59%) with a significant difference (p-value = 0.0077). In addition, Anaplasma centrale (18.75%) more infects sheep in the locality of Lokoli followed by Korhogo (4.69%) with a significant difference (p-value = 0.00037)

    Trypanosomiasis prevalence in the cotton basin in the Sudanese zone of CĂ´te d'Ivoire

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    La prĂ©valence trypanosomienne a Ă©tĂ© Ă©valuĂ©e dans les cheptels du bassin cotonnier en zone soudanaise de CĂ´te d’Ivoire. Les prĂ©lèvements de sang ont Ă©tĂ© effectuĂ©s du 22 avril au 21 mai 2016, sur 582 bovins dont 374 mâles et 195 femelles, appartenant aux phĂ©notypes MĂ©rĂ©, ZĂ©bu et Ndama. Les espèces de trypanosomes ont Ă©tĂ© d’abord identifiĂ©es sur le terrain Ă  partir de frottis sanguins, puis caractĂ©risĂ©es au laboratoire au moyen de la PCR (Polymerase Chain Reaction). Les trois espèces diagnostiquĂ©es sont, dans l’ordre dĂ©croissant de leur taux de prĂ©valence, Trypanosoma congolense groupe savane, Trypanosoma vivax et Trypanosoma brucei brucei. Le plus grand nombre de bovins infectĂ©s provient de Korhogo, suivi de M’BenguĂ©, puis de FerkessĂ©dougou. Le taux d’infection est plus faible et du mĂŞme ordre Ă  Katiola, Koumbala, Diawara et NiellĂ©. Les MĂ©rĂ© sont les plus infectĂ©s par rapport aux ZĂ©bu et aux Ndama. Ces derniers sont les plus faiblement infectĂ©s. Chez les Ndama, la trypanotolĂ©rance apparaĂ®t encore plus efficiente chez les animaux de moins de 8 ans. Les MĂ©rĂ© montrent une forte parasitĂ©mie due Ă  Trypanosoma congolense ou Ă  Trypanosoma vivax, sans toutefois faire la maladie. Mots clĂ©s : TAA, PrĂ©valence, Zone soudanaise, CĂ´te d’IvoireTrypanosomiais prevalence was evaluated in the flocks of the cotton basin in the Sudanese zone of CĂ´te d'Ivoire. The blood samples were taken from April 22 to May 21, 2016, on 582 cattle including 374 males and 195 females, belonging to the phenotypes MĂ©rĂ©, ZĂ©bu and Ndama. Trypanosome species were first identified in the field from blood smears and then characterized in the laboratory using PCR (Polymerase Chain Reaction). The three species diagnosed are, in decreasing order of their prevalence rate, Trypanosoma congolense savannah group, Trypanosoma vivax and Trypanosoma brucei brucei. Most of the infected cattle come from Korhogo, followed by M'BenguĂ© and then FerkessĂ©dougou. The infection rate is lower and of the same order in Katiola, Koumbala, Diawara and NiellĂ©. MĂ©rĂ© are the most infected compared to Zebu and Ndama. The latter are the most weakly infected. For the Ndama, trypanotolerance appears to be even more efficient in animals less than 8 years old. The MĂ©rĂ© show a strong parasitaemia due to Trypanosoma congolense or Trypanosoma vivax, without however being sick. Keywords : AAT, Prevalence, Sudanese zone, CĂ´te d’Ivoir

    Risk of potential transmission of bacterial infections by synanthropic flies in urban areas of CĂ´te d'Ivoire

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    Du 11 janvier au 25 fĂ©vrier 2022, des mouches ont Ă©tĂ© capturĂ©es dans la ville de Korhogo, en zone soudanaise de CĂ´te d’Ivoire. Le but Ă©tait d’évaluer le rĂ´le potentiel de ces mouches dans la transmission des infections bactĂ©riennes aux hommes et aux animaux dans la zone urbaine. Les captures ont Ă©tĂ© opĂ©rĂ©es Ă  l’aide d’un piège aseptisĂ© Ă  appât alimentaire, dans quatre types d'Ă©cosystèmes (restaurants, cantines scolaires, marchĂ©s, abattoirs, lieux d’élevage). Deux espèces de mouches synanthropes ont Ă©tĂ© capturĂ©es et identifiĂ©es : Musca domestica et Chrysomya albiceps. Elles ont permis de caractĂ©riser, au laboratoire, 7 espèces de bactĂ©ries : Escherichia coli, Salmonella sp., Proteus sp., Klebsiella sp., Staphylococcus sp., Bacillus sp. et Pseudomonas sp. Il en rĂ©sulte que, dans la ville de Korhogo, le risque de contamination bactĂ©rienne est Ă©levĂ©, particulièrement en zones d’élevage et dans les restaurants. Le risque est tout aussi important, mais Ă  un degrĂ© moindre et dans des proportions comparables, dans les marchĂ©s et les abattoirs. Au niveau des cantines scolaires, le risque apparait le plus faible. Cependant, les enfants courent toujours un danger parce que les proportions des mouches synanthropes et des bactĂ©ries pathogènes qui y circulent, restent notables. Mots clĂ©s : Mouches synanthropes, BactĂ©ries, Zone urbaine, CĂ´te d’IvoireFrom 11 January to 25 February 2022, flies were caught in the city of Korhogo, in the Sudanese area of CĂ´te d'Ivoire. The aim was to assess the potential role of these flies in the transmission of bacterial infections to humans and animals in urban areas. Catches were made using a sanitized food bait trap in four types of ecosystems (restaurants, school canteens, markets, slaughterhouses, livestock areas). Two species of synanthropic flies were captured and identified : Musca domestica and Chrysomya albiceps. They were used to characterize 7 species of bacteria in the laboratory : Escherichia coli, Salmonella sp., Proteus sp., Klebsiella sp., Staphylococcus sp., Bacillus sp. and Pseudomonas sp. The result is that the risk of bacterial contamination in the town of Korhogo is high, especially in livestock areas and restaurants. The risk is just as great, but to a lesser extent and in comparable proportions, in markets and slaughterhouses. In school canteens, the risk appears to be the lowest. However, children are still in danger because the proportions of synanthropic flies and pathogenic bacteria that circulate there remain significant. Keywords: Synanthropic flies, Bacteria, Urban area, CĂ´te d’Ivoir

    Genetic Diversity of Trypanosomes Infesting Cattle from Savannah District in North of Côte d’Ivoire Using Conserved Genomic Signatures: rRNA, ITS1 and gGAPDH

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    The potential danger to livestock from African animal trypanosomiasis is well known. However, the trypanosome species circulating in cattle and their genetics are poorly understood. After different alignments according to three regions (ITS1, gGAPDH and rRNA gene) of the trypanosome genome, phylogenetic analyses were used to show the genetic diversity of the different species that were circulating in the cattle in three regions (Bagoue, Poro and Tchologo) of Côte d’Ivoire. These analyses were performed by alignment of ITS1; by alignment of partial 18S, ITS1, 5.8S, ITS2 and partial 28S rRNA genes; and by alignment of gGAPDH gene with sequences of Trypanosomes found in GenBank. Three species were identified (T. vivax, T. theileri and T. congolense) in the cattle in the three northern regions of Côte d’Ivoire. T. vivax and T. theileri were the most abundant species in the present study. Contrary to the other primers used in this study, the ITS1 primers were not able to amplify T. theileri. We observed mixed infections between T. theileri and the other two species identified (T. vivax and T. congolense). As far as primers are concerned, in some cases, rRNA was able to identify the same species of trypanosomes that the ITS1 and gGAPDH primers were able to identify. Two main distinct groups of T. theileri complex were identified. The T. congolense and T. vivax strains were close to African strains, such as those from Kenya, Nigeria and Cameroon, unlike the T. theileri strain. Three trypanosome species (T. vivax, T. theileri and T. congolense) circulate in cattle in the Savannah district of Côte d’Ivoire. The genetic diversity of the trypanosome species encountered in this study cannot be classified as intraspecies according to geographical area and breed of cattle they infect
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